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World Journal of Gastroenterology 2014年第16期20卷 4771-4777页

作者： Yan-Min Zhang Shu-Kun Zhang nai-qiang cui Tianjin Medical University Intensive Care UnitTianjin Nankai Hospital Tianjin Nankai Hospital Department of Surgery Tianjin Nankai Hospital

AIM: To investigate whether mesenteric lymph from rats with severe intraperitoneal infection (SII) induces lung injury in healthy rats.

关键词： Severe intraperitoneal infection, Mesenteric lymph, Acute lung injury, Toll-like receptor 4, Nuclear factor κ B

Phasic study of intestinal homeostasis disruption in experimental intestinal obstruction

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World Journal of Gastroenterology 2014年第25期20卷 8130-8138页

作者： Xiang-Yang Yu Chang-Lin Zou Zhen-Li Zhou Tao Shan Dong-Hua Li nai-qiang cui Department of Gastrointestinal Surgery Nankai Hospital Graduate School of Tianjin Medical University Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine Department of Hepatopancreatobiliary Surgery Nankai Hospital

AIM: To investigate the phasic alteration of intestinal homeostasis in an experimental model of intestinal obstruction.

关键词： Intestinal obstruction Rabbit model Homeostasis disruption Intestinal epithelial cells Intestinal microorganisms Intestinal immune system

Upreguiation of human telomerase reverse transcriptase mRNA expression by in vitro transfection of hepatitis B virus X gene into human hepatocarcinoma and cholangiocarcinoma cells

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World Journal of Gastroenterology 2005年第36期11卷 5627-5632页

作者： Zhen-Liang Qu Sheng-Quan Zou nai-qiang cui Xian-Zhong Wu Ming-Fang Qin Di Kong Zhen-Li Zhou Department of surgery Tianjin Nankai Hospital Tianjin 300100 China Department of Surgery Tongji Hospital Wuhan430030 Hubei Province China

AIM： To study the changes of human telomerase reverse transcriptase （hTERT） mRNA expression in human hepatocarcinoma cell lines （HepG2） and cholangiocarcinoma cell lines （QBC939） after HBx gene transfection and to illustrate the significance of transcriptional regulation of hTERT gene by HBx gene in the carcinogenesis. METHODS： HepG2 and QBC939 cell lines were cultured and co-transfected with eukaryotic expression vector containing the HBx coding region and cloning vector containing enhanced green fluorescent protein （EGFP） coding sequence using lipid-mediated gene transduction technique. Thirty-six hours after transfection, EGFP expression in cells was used as the indicator of successful transfection. Flow cytometry was performed to determine the transfection efficiency. Cells were harvested and total RNA was extracted using TRIzol reagent. The expression of hTERT mRNA in HepG2 and QBC939 cell lines was assayed by reverse transcriptionpolymerase chain reaction. The expression of HBx protein in both cell lines was detected by immunocytochemical staining and Western blotting. RESULTS： Flow cytometry showed that the transfection efficiency was 46.4% in HepG2 cells and 29.6% in QBC939 cells for both HBx gene expression vector and blank vector. The expression of hTERT mRNA was meaningfully increased in HepG2 and QBC939 cell lines when transfected with HBx gene expression vector compared to those transfected with OPTI-MEM medium and blank vector. Immunocytochemical staining and Western blotting revealed HBx protein expression in HepG2 and QBC939 cells only when transfected with HBx gene. CONCLUSION： HBx gene transfection can upregulate the transcriptional expression of hTERT mRNA. The transactivation of hTERT gene by HBx gene is a newfound mechanism for pathogenesis of hepatocarcinomas and cholangiocarcinomas after HBV infection. 2005 The WJG Press and Elsevier Inc. All rights reserved

关键词： Hepatocholangiocarcinoma Human telomerasereverse transcriptase Gene expression Hepatitis B virus X protein

Role of Kupffer cells in acute hemorrhagic necrotizing pancreatitis-associated lung injury of rats

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World Journal of Gastroenterology 2006年第3期12卷 403-407页

作者： Hong-Bin Liu nai-qiang cui Dong-Hua Li Chang Chen Department of Pharmacology Tianjin Institute of Acute Abdominal Diseases Tianjin 300100China Second Department of Surgery Tianjin Institute of Acute Abdominal Diseases Tianjin 300100China

AIM： To investigate the role of Kupffer cells （KCs） in acute hemorrhagic necrotizing pancreatitis-associated lung injury （AHNP-U）. METHODS： Forty-two rats were allocated to four groups [sham operation, AHNP model, gadolinium chloride （GdCl3） pretreatment, GdCl3 control]. In GdCl3 pretreatment group, GdCl3 was administered by caudal vein injection 24 h before the AHNP model induction. Blood from the iliac artery, alveolar macrophages and tissues from the pancreas and lung, were collected in six animals per group 3 and 6 h after acute pancreatitis induction. TNF-α, IL-1 of Lserum, myeloperoxidase （MPO） of lung tissue, NF-κB activation of alveolar macrophages were detected. Serum AST and ALT in sham operation group and GdCl3 control group were tested. In addition, histopathological changes of the pancreas and lung were observed under light microscope. RESULTS： MPO of lung tissue and TNF-α, IL-1 levels of serum were all reduced significantly in GdCl3 pretreatment group compared to those in AHNP group （P〈0.01）. NF-KB activation of alveolar macrophages was also attenuated significantly in GdCl3 pretreatment group compared to that in AHNP group （P〈0.01）. The pathological injury of the lung was ameliorated obviously in GdCl3 pretreatment group compared to that in AHNP group. Nevertheless, the serum amylase level did not reduce and injury of the pancreas was not prevented in GdCl3 pretreatment group. CONCLUSION： Pulmonary injury induced by AHNP is mediated by KC activation and AHNP-LI can be significantly ameliorated by pretreatment with GdCh and KCs play a vital role in AHNP-LI.

关键词： Pancreatitis-associated lung injury Kupffer cell NF-κB Gadolinium chloride

Overexpression of sterol carrier protein-2 mRNA in patients with cholesterol gallstones

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Hepatobiliary & Pancreatic Diseases International 2005年第1期4卷 117-120页

作者： nai-qiang cui, Shu-Kun Zhang, Yun-Feng cui, Dong-Hua Li,Chang Chen and Xian-Zhong Wu Tianjin, China Institute of Acute Abdominal Diseases and Department of Surgery Department of Surgery Tianjin Nankai Hospital Tianjin 300100 China. yunfengcuidoctor@***

BACKGROUND: Hypersecretion of biliary cholesterol is believed to be one of the important causes of lithogenic bile. Sterol carrier protein-2 ( SCP2 ) participates in choles- terol trafficking and metabolism and may play a key role in cholesterol gallstone formation. This study was undertaken to investigate the expression of liver SCP2 mRNA in pa- tients with cholesterol gallstone and those patients with non-cholesterol gallstone. METHODS: The expression of liver SCP2 mRNA was studi- ed in 36 patients with cholesterol gallstone and 30 patients with non-cholesterol gallstone by reverse transcription-poly- merase chain reaction (RT-PCR). RESULT: The expression of SCP2 mRNA was increased more significantly in patients with cholesterol gallstone than in patients with non-cholesterol gallstone. CONCLUSION: The SCP2 gene was overexpressed in pa- tients with cholesterol gallstone, indicating that SCP2 may be one of the important causes of cholesterol gallstone.

关键词： SCP2 mRNA expression lithogenic biles cholesterol gallstone

Liver Cirrhosis and Intestinal Bacterial Translocation

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国际感染病学（电子版） 2014年第3期3卷 133-138页

作者： Tao Lan Bao-chun Chen Li-ping Fu Zhi-juan Li Xiang-jun Wu nai-qiang cui Graduate School of Tianjin University of Traditional Chinese Medicine Cangzhou People's Hospital Affiliated to Southern Medical University

Intestinal barrier dysfunction, facilitating translocation of bacteria and bacterial products, plays an important role in the pathophysiology of liver cirrhosis and its complications. Intestinal defense system including microbial barrier, immunologic barrier, mechanical barrier, chemical barrier, plays an important role in the maintenance of intestinal function. Under normal circumstances, the intestinal barrier can prevent intestinal bacteria through the intestinal wall from spreading to the body. Severe infection, trauma, shock, cirrhosis, malnutrition, immune suppression conditions, intestinal bacteria and endotoxin translocation, can lead to multiple organ dysfunction. The intestinal microflora is not only involved in the digestion of nutrients, but also in local immunity, forming a barrier against pathogenic microorganisms. The derangement of the gut microflora may lead to microbial translocation, defined as the passage of viable microorganisms or bacterial products from the intestinal lumen to the mesenteric lymph nodes and other extraintestinal sites. In patients with cirrhosis, primary and intestinal flora imbalance, intestinal bacterial overgrowth, intestinal mucosal barrier dysfunction, endotoxemia is associated with weakened immunity.

关键词： Cirrhosis Intestinal barrier Bacterial translocation Endotoxemia

家族性胆固醇结石患者肝组织HMGCR和SCP_2mRNA的表达

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世界华人消化杂志 2005年第9期13卷 1115-1118页

作者：崔云峰崔乃强李东华张琚天津市南开医院急腹症研究所天津市300100 南开大学分子生物学研究所天津市300071

目的:研究家族遗传性胆囊胆固醇结石患者、非家族遗传性胆囊胆固醇结石患者和非胆固醇结石患者肝组织HMGCoA还原酶(HMGCR)、固醇携带蛋白2(SCP2)基因mRNA的表达情况,进而明确二者在胆石形成中的作用.方法:选取2003-08/2004-08天津市南... 详细信息

目的:研究家族遗传性胆囊胆固醇结石患者、非家族遗传性胆囊胆固醇结石患者和非胆固醇结石患者肝组织HMGCoA还原酶(HMGCR)、固醇携带蛋白2(SCP2)基因mRNA的表达情况,进而明确二者在胆石形成中的作用.方法:选取2003-08/2004-08天津市南开医院外科住院病例90例,均取得患者及家属知情同意,其中家族遗传性胆囊胆固醇结石患者28例,非家族遗传性胆囊胆固醇结石患者30例,非胆固醇结石患者32例,术中肝活检,行肝组织总RNA提取,逆转录反应合成cDNA,设计引物,进行聚合酶链反应(PCR)扩增HMGCR和SCP2基因的目的片段,检测肝组织mRNA的表达水平的不同.结果:家族遗传性和非家族遗传性胆固醇结石组HMGCR,SCP2mRNA表达水平较非胆固醇结石组升高;其中HMG(:RmRNA表达在家族遗侉}生胆固醇结石组为1.9269±0.2134,非家族遗传性组为1.9791±0.2524,二者均高于非胆固醇结石组0.7730±0.1530,差异有显著性(P<0.05);SCP2mRNA表达在家族遗传性胆固醇结石组为0.8908±0.1649,非家族遗传性组为0.7503±0.1004,二者均高于非胆固醇结石组0.5205±0.1900,差异有显著性(P<0.05);家族遗传性胆固醇结石SCP2mRNA表达水平较非家族遗传性胆固醇结石组升高,差异有显著性(P<0.05),而HMGCRmRNA在家族遗传性和非家族遗传性胆固醇结石组两组间无统计学差别.结论:胆囊胆固醇结石患者肝组织HMGCR,SCP2mRNA表达水平升高是胆囊胆固醇结石形成的重要原因.

关键词：结石患者肝组织聚合酶链反应(pCR) mRNA表达家族遗传性家族性胆囊胆固醇结石形成 SCP2 HMGCoA 基因mRNA 总RNA提取逆转录反应显著性表达情况住院病例医院外科胆石形成家属知情 cDNA 设计引物 P2基因水平还原酶

早期通里攻下法对重症急性胰腺炎结局影响的系统评价

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世界华人消化杂志 2009年第10期17卷 1042-1047页

作者：苗彬崔乃强李忠廉马涛赵光王心天津医科大学南开临床学院南开医院全国胆胰疾病诊疗中心天津市300100

目的:评价通里攻下法治疗重症急性胰腺炎(severeacutepancreatitis,SAP)的疗效.方法:检索2004-01/2008-12国内生物医学期刊发表的有关中医通里攻下法治疗SAP的随机对照临床试验(RCT)文献.根据入选及排除标准,有8项临床试验,628例SAP患者... 详细信息

目的:评价通里攻下法治疗重症急性胰腺炎(severeacutepancreatitis,SAP)的疗效.方法:检索2004-01/2008-12国内生物医学期刊发表的有关中医通里攻下法治疗SAP的随机对照临床试验(RCT)文献.根据入选及排除标准,有8项临床试验,628例SAP患者(中药治疗组335例,对照组293例)纳入本研究.使用RevMan4.2软件合并分析纳入研究的SAP患者的首次排便时间、住院时间、并发症发生率、手术率及病死率等结局指标.结果:中药治疗组的首次排便时间,住院天数的标准化均数差均明显低于对照组,且差异有显著性(SMD=-2.34,-1.91,均P<0.01).中药治疗组的并发症发生率、手术率、病死率的优势比均明显低于对照组,且差异有显著性(OR=0.36,0.41,0.31,均P<0.01).结论:应用通里攻下治疗能显著缩短重症急性胰腺炎患者的首次排便时间、住院时间,明显降低手术率、并发症发生率、病死率.

关键词：重症急性胰腺炎中药疗效评价病死率系统评价

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清胰片在SAP恢复期中的治疗价值

世界华人消化杂志 2009年第13期17卷 1374-1377页

作者：苗彬崔乃强李忠廉马涛赵光王心天津医科大学南开临床学院南开医院全国胆胰疾病诊疗中心天津市300100

目的:探讨清胰片对重症急性胰腺炎(severe acute pancreatitis,SAP)患者恢复期的治疗价值.方法:选择1997-01/2007-12我院符合纳入标准的SAP痊愈患者294例,失访及剔除22例,合格病例为272例,随机分为中药组和对照组,观察两组急性胰腺炎(ac... 详细信息

目的:探讨清胰片对重症急性胰腺炎(severe acute pancreatitis,SAP)患者恢复期的治疗价值.方法:选择1997-01/2007-12我院符合纳入标准的SAP痊愈患者294例,失访及剔除22例,合格病例为272例,随机分为中药组和对照组,观察两组急性胰腺炎(acute pancreatitis,AP)复发及营养状态.结果:SAP患者出院12mo内AP复发率为46.3%(126/272).中药组出院12mo内AP的复发率较对照组显著下降(39.9%vs53.0%,P<0.05).中药组出院后6、12mo营养状态受损评分均较对照组明显减低(t=2.23,2.77,P<0.05或0.01).结论:出院后3mo内继续服用中药清胰片治疗可以减少SAP恢复期的远期AP的复发并可改善其营养状态.

关键词：重症急性胰腺炎复发中药营养

乳头气囊扩张联合乳头括约肌切开治疗胆总管结石的疗效及其对结石复发的影响

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世界华人消化杂志 2018年第4期26卷 263-269页

作者：冯健崔乃强蔡旺李湧郑明伟张辰天津中医药大学天津市南开医院日间手术中心天津市300100 南开医院肝胆胰外科天津市300100

目的评价应用乳头气囊扩张(endoscopic papillary balloon dilatation,EPBD)联合乳头括约肌切开(endoscopic sphincterotomy,EST)治疗胆总管结石的疗效及其对结石复发的影响.方法选择2014-01/2016-01于天津南开医院行内镜逆行胰胆管造影术(endoscopic retrograde cholangiopan creatography,ERCP)治疗的胆总管结石患者100例,采用随机数字表法分为对照组和实验组,分别给予经ERCP下EST取石术和乳头括约肌小切开(limited endoscopic sphincterotomy,LEST)联合EPBD,比较两组患者的一次成功率和总成功率,ERCP术后并发症发生率,术后进行随访,绘制患者术后结石复发的Kaplan-Meier生存曲线,并应用Cox回归模型探究影响患者术后复发的危险因素.结果对照组和实验组的结石最大直径(t=-1.055,P=0.294)、多发结石比例(χ~2=0.644,P=0.422)、胆管最大直径(t=-0.820,P=0.414)以及取石总成功率(94.0%和100.0%,χ~2=1.375,P=0.241)间均无统计学差异,对照组和实验组的1次取石成功率分别为84.0%和96.0%,差异有统计学意义(χ~2=4.332,P=0.037).对照组和实验组ERCP术后并发症的发生率分别为17.02%和6.0%,差异有统计学意义(χ~2=4.337,P=0.036).对取石成功的97例患者进行随访,共发生结石复发11例,其中,对照组和实验组ERCP术后并发症的发生率分别为17.02%和6.0%,差异无统计学意义(χ~2=2.927,P=0.087),Log-rank显示,实验组的胆结石复发风险显著低于对照组(HR=0.285,95%CI:0.095-0.888,P=0.044).多因素Cox回归分析显示,多次内镜操作、胆总管成角≤145o和EST是ERCP术后复发的危险因素.结论 EPBD联合LEST治疗胆总管结石的一次成功率和安全性较高,结石远期复发风险较低;多次内镜操作、胆总管成角≤145o是ERCP术后复发的危险因素.

关键词：胆总管结石经内镜逆行性胰胆管造影术乳头气囊扩张乳头括约肌切开