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A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

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Bio-Design and Manufacturing 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.Dos Santos P.J.Dijkstra C.Mota J.Bauer H.Ferreira Rui L.Reis n.neves S.Camarero-Espinosa M.B.Baker L.Moroni Department of Complex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal POLYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(PCL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight PCL75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight PCL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a bone morphogenetic protein 2(BMP-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMSCs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of BMP receptor II(BMPRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive manufacturing Blending Surface functionalization Surface density Click chemistry Human

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葡萄牙森林植物检疫现状

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国外林业 1989年第1期 27-28页

作者： n.neves 藏秀强宋玉双

在过去的4年中,由于真菌病害的危害,造成森林在经济上的损失有所增加。在苗圃和针叶林做了调查和详细记录,亚速群岛和葡萄牙大陆两处都发现并鉴定了一大批病害,结论如下:森林火灾看来会诱导某些常与小蠹虫有关的病害发生,并对今后的发... 详细信息

在过去的4年中,由于真菌病害的危害,造成森林在经济上的损失有所增加。在苗圃和针叶林做了调查和详细记录,亚速群岛和葡萄牙大陆两处都发现并鉴定了一大批病害,结论如下:森林火灾看来会诱导某些常与小蠹虫有关的病害发生,并对今后的发展做了论述。

关键词：森林群岛针叶森林火灾火烧迹地葡萄牙南欧蠢虫林地农业用地病原菌林分海岸松苗圃寄生菌

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Replication of natural surface topographies to generate advanced cell culture substrates

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Bioactive Materials 2023年第10期28卷 337-347页

作者： n.O.Monteiro J.F.Fangueiro R.L.Reis n.M.neves 3B’s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkParque de Ciencia e Tecnologi1Zona Industrial da Gandr14805-017BarcoGuimaraesPortugal ICVS/3B’s-PT Government Associate Laboratory Brag1Guimar˜aesPortugal

Surface topographies of cell culture substrates can be used to generate in vitro cell culture environments similar to the in vivo cell niches. In vivo, the physical properties of the extracellular matrix (ECM), such as its topography, provide physical cues that play an important role in modulating cell function. Mimicking these properties remains a challenge to provide in vitro realistic environments for cells. Artificially generated substrates’ topographies were used extensively to explore this important surface cue. More recently, the replication of natural surface topographies has been enabling to exploration of characteristics such as hierarchy and size scales relevant for cells as advanced biomimetic substrates. These substrates offer more realistic and mimetic environments regarding the topographies found in vivo. This review will highlight the use of natural surface topographies as a template to generate substrates for in-vitro cell culture. This review starts with an analysis of the main cell functions that can be regulated by the substrate’s surface topography through cell-substrate interactions. Then, we will discuss research works wherein substrates for cell biology decorated with natural surface topographies were used and investigated regarding their influence on cellular performance. At the end of this review, we will highlight the advantages and challenges of the use of natural surface topographies as a template for the generation of advanced substrates for cell culture.

关键词： Biomimetic topography Cell culture substrates natural surface topographies

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Detection of flock movement and behaviour of broiler chickens at different feeders using image analysis

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Information Processing in Agriculture 2015年第3期2卷 177-182页

作者： Diego Pereira neves Saman Abdanan Mehdizadeh Matthew Tscharke Irenilza de Alencar nääs Thomas Michael Banhazi Structure&Motion Laboratory Royal Veterinary CollegeUniversity of LondonLondonUnited Kingdom Department of Mechanics of Biosystems Engineering College of Agricultural Engineering and Rural DevelopmentRamin University of Agriculture and Natural Resources of KhuzestanMollasaniAhvazKhuzestanIran nCEA University of Southern QueenslandToowoomba CampusQLDAustralia Agricultural Engineering College State University of CampinasCampinasSPBrazil nCEA and Faculty of Health Engineering and ScienceUniversity of Southern QueenslandToowoomba CampusQLDAustralia

The behaviour of broiler chickens has been extensively studied as a function of stocking density and environmental conditions,but limited information was reported in the scientific literature about the effect of feeder type on birds’feeding *** main objective of this study was to assess the effects of three different feeder types in relation to the birds’behaviour in its *** analysed feeders were:tube-type with partition grid(F1);tube-type without partition grid(F2);and automatic type with partition grid(F3).The considered variables were:occupied area(OA);activity index(AI)(flock movement);total birds presented in the area(TB);and birds effectively eating(EE).OA and AI were calculated by computational image analysis while TB and EE were manually *** results indicated that the feeder type could have influenced the birds’behaviour regarding to OA(R2=0.56),TB(R2=0.48),and EE(R2=0.40),but AI(R2=0.01)was not found to be directly influenced by the feeder type.A higher percentage of birds effectively eating were found in F2(86.4%),which was the one with the largest free area to access the *** average number of total birds was found in F1,but with a lower percentage of individuals effectively eating(63.3%),which means that birds were nearby this feeder performing other *** the assessed feeders were in the same house under the same conditions,it can be suggested that not only the free area to access the feed but potentially the design of feeders could have influenced the birds’feeding *** real beneficial effect of the adoption of partition grid on feed trays is still uncertain,and it is also unclear whether the financial value of reduced wastage would compensate the possible reduction in feed intake.

关键词： Activity index Animal behaviour Feeding Image processing Motion Occupied area

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Hyperbolic Conservation Laws on *** Error Estimate for Finite Volume Schemes

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Acta Mathematica Sinica,English Series 2009年第7期25卷 1041-1066页

作者： Philippe G.LeFLOCH Baver OKUTMUSTUR Wladimir neves Laboratoire Jacques-Louis Lions Centre National de la Recherche ScientifiqueUniversité de Paris 6 Instituto de Matemática Universidade Federal do Rio de Janeiro C.P. 68530Cidade Universitária 21945-970

Following Ben-Artzi and LeFloch, we consider nonlinear hyperbolic conservation laws posed on a Riemannian manifold, and we establish an L1-error estimate for a class of finite volume schemes allowing for the approximation of entropy solutions to the initial value problem. The error in the L1 norm is of order h1/4 at most, where h represents the maximal diameter of elements in the family of geodesic triangulations. The proof relies on a suitable generalization of Cockburn, Coquel, and LeFloch＇s theory which was originally developed in the Euclidian setting. We extend the arguments to curved manifolds, by taking into account the effects to the geometry and overcoming several new technical difficulties.

关键词： Hyperbolic conservation law entropy solution finite volume scheme error estimate discrete entropy inequality convergence rate

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Mercury in aquatic fauna contamination:A systematic review on its dynamics and potential health risks

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Journal of Environmental Sciences 2019年第10期31卷 205-218页

作者： Paloma de Almeida Rodrigues Rafaela Gomes Ferrari Luciano neves dos Santos Carlos Adam Conte Junior Molecular and Analytical Laboratory Center Department of Food TechnologyFaculty of VeterinaryUniversidade Federal FluminenseNiteróiBrazil Chemistry Institute Food Science ProgramUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil national Institute of Health Quality Control Fundacaeo Oswaldo CruzRio de JaneiroBrazil Laboratory of Theoretical and Applied Ichthyology Department of Ecology and Marine ResourcesUniversidade Federal do Estado do Rio de JaneiroRio de JaneiroBrazil

Mercury is an important pollutant,released into aquatic ecosystems both naturally and by anthropogenic *** element is transferred to aquatic organisms in different ways,causing potential health *** addition,mercury can be accumulated by humans,especially through the consumption of contaminated *** systematic review aims to present mercury pathways,the major routes through which this element reaches the aquatic environment and its transformations until becoming available to living animals,leading to bioaccumulation and biomagnification *** key biotic and abiotic factors affecting such processes,the impact of mercury on animal and human health and the issue of seafood consumption as a source of chronic mercury contamination are also addressed.A total of 101 articles were retrieved from a standardized search on three databases(PubMed,Emabse,and Web of Science),in addition to 28 other studies not found on these databases but considered fundamental to this review(totaling 129 articles).Both biotic and abiotic factors display fundamental importance in mediating mercurial dynamics,i.e.,muscle tropism,and salinity,***,mercurial contamination in aquatic environments affects animal health,especially the risk of extinction species and also on human health,with methylmercury the main mercury species responsible for acute and chronic symptomatology.

关键词： Mercury levels Seafood Methylmercury Public health Aquatic environment

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The phylogeny of selected Phylloporus species,inferred from nUC-LSU and ITS sequences,and descriptions of new species from the Old World

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Fungal Diversity 2012年第4期55卷 109-123页

作者： Maria Alice neves Manfred Binder Roy Halling David Hibbett Kasem Soytong Departamento de Botânica Universidade Federal de Santa CatarinaFlorianópolisSC 88040-900Brazil Biology Department Lasry Center for BiosciencesClark University15 Maywood StreetWorcesterMA 01601USA Institute of Systematic Botany The New York Botanical GardenBronxNY 10458-5126USA Faculty of Agricultural Technology King Mongkut’s Institute of TechnologyLadkrabangBangkokThailand

The phylogeny of Phylloporus(Boletaceae)has not been well studied,and the taxonomic relationships of this genus have varied considerably among *** following study presents phylogenetic relationships of Phylloporus based on two nuclear ribosomal DnA regions,ITS and *** ITS dataset includes 39 collections and the LSU dataset contains 50 collections of Phylloporus.A combined analysis of both genes did not resolve the deeper nodes in the phylogeny,but the results suggest that Phylloporus is monophyletic and a sister group of the Xerocomus subtomentosus *** lamellate hymenophore configuration is a synapomorphy that distinguishes Phylloporus from the other genera in the *** placement of a lamellate genus within Boletaceae suggests that hymenophore evolution is not well understood in the *** is the first phylogeny of Phylloporus and includes 20 species from different geographic *** taxa of Phylloporus from the Old World are here *** cyanescens is a new combination for an Australasian taxon formerly named as a variety of ***(Massee)*** pumilus is described from Indonesia,and 4 species are described from Thailand:***,***,***,and ***.

关键词： Boletaceae Agaricomycotina Taxonomy Distribution

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network of GRAS transcription factors in plant development,fruit ripening and stress responses

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Horticulture Research 2023年第12期10卷 286-303页

作者： Catarina neves Beatriz Ribeiro Rute Amaro Jesús Expósito Jérôme Grimplet Ana Margarida Fortes BioISI-Biosystems and Integrative Sciences Institute Faculty of SciencesUniversity of LisbonCampo Grande1749-016 LisboaPortugal Centro de Investigación y Tecnología Agroalimentaria de Aragón(CITA) Departamento de Ciencia VegetalGobierno de AragónAvda.Montañana 93050059 ZaragozaSpain Instituto Agroalimentario de Aragón-IA2(CITA-Universidad de Zaragoza) Calle Miguel Servet 17750013 ZaragozaSpain

The plant-specific family of GRAS transcription factors has been wide implicated in the regulation of transcriptional reprogramming associated with a diversity of biological functions ranging from plant development processes to stress *** analyses of GRAS transcription factors supported by in silico structural and comparative analyses are emerging and clarifying the regulatory networks associated with their biological *** this review,a detailed analysis of GRAS proteins’structure and biochemical features as revealed by recent discoveries indicated how these characteristics may impact subcellular location,molecular mechanisms,and *** issues associated with GRAS classification into different subfamilies in diverse plant species even in the presence of robust genomic resources are discussed,in particular how it affects assumptions of biological *** into the mechanisms driving evolution of this gene family and how genetic and epigenetic regulation of GRAS contributes to subfunctionalization are ***,this review debates challenges and future perspectives on the application of this complex but promising gene family for crop improvement to cope with challenges of environmental transition.

关键词： function GRAS plant

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How does the brown mussel Perna perna respond to environmental pollution?A review on pollution biomarkers

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Journal of Environmental Sciences 2022年第1期34卷 412-428页

作者： Fernanda Silva dos Santos Raquel A.F.neves Mirian Araújo Carlos Crapez Valéria Laneuville Teixeira natascha Krepsky Fluminense Federal University(UFF) Institute of BiologyGraduate Program in Science and BiotechnologyMario Santos Braga Streets/n.CentroNiteróiRJ CEP 24.020-141Brazil Federal University of the State of Rio de Janeiro(UnIRIO) Institute of Biosciences(IBIO)Graduate Program in Neotropical Biodiversity(PPGBIO)Pasteur Avenue458.UrcaRio de JaneiroRJ CEP 22.290-255Brazil Fluminense Federal University(UFF) Institute of BiologyGraduate Program in Marine Biology and Coastal EnvironmentsMario Santos Braga Streets/n.CentroNiteróiRJ CEP 24.020-141Brazil Federal University of the State of Rio de Janeiro(UnIRIO) Institute of Biosciences(IBIO)Graduate Program in Ecotourism and ConservationPasteur Avenue458.UrcaRio de JaneiroRJ CEP 22.290-255Brazil

The brown mussel Perna perna(Linnaeus,1758)is a valuable resource for aquaculture in tropical and subtropical coastal *** presents desirable characteristics for biomonitoring,including being sessile,widely distributed and abundant,and is a filter-feeder able to accumulate several classes of pollutants(e.g.,metals,hydrocarbons,among others).Mussels’biological responses to pollution exposure can be measured as biomarkers,which include alterations ranging from molecular to physiological levels,to estimate the degree of environmental contamination and its effects on *** full review compiles two decades(2000–2020)of literature concerning biological effects on *** mussel caused by environmental pollutants(i.e.,metals,hydrocarbons,and emerging pollutants),considering environmental and farm-based *** markers related to mussels’oxidative status were efficient for the biomonitoring of metals(i.e.,antioxidant enzymes associated with oxidative damage in biomolecules).Genotoxicity and cytotoxicity indicators(i.e.,comet,micronucleus,and neutral red assays)provided a depiction of hydrocarbon *** neutral red assay gave a time-concentration cytotoxic response to a wide range of pollutants,including emerging pollutants(e.g.,pharmaceuticals and biocides)and *** perna hemocyte parameters provided a useful approach for biocide *** paper summarizes useful biomarkers from molecular to physiological levels in this mussel species used to identify and quantify the degree of coastal *** integrated biomarker analysis may provide a way to overcome possible biomarker variations and assess multi-polluted ***,it is necessary to investigate biomarker variations according to natural factors(e.g.,season and gonad maturation stage)to standardize them for trustworthy biomonitoring.

关键词： Biological indexes Biomonitoring Emerging pollutants Hydrocarbons Metals Sentinel species

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Childhood trauma and factors associated with depression among inpatients with cardiovascular disease

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World Journal of Psychiatry 2017年第2期7卷 106-113页

作者： Felipe José nascimento Barreto Frederico Duarte Garcia Paulo Henrique Teixeira Prado Paulo Marcos Brasil Rocha nádia Souza Las Casas Felipe Barbosa Vallt Humberto Correa Maila Castro Lourenco neves Department of Mental Health Federal University of Minas Gerais Postgraduation Program in Molecular Medicine School of Medicine Federal University of Minas Gerais Molecular Medicine national Institute of Science and Technology Avenida Alfredo Balena Federal University of Minas Gerais Hospital das Clínicas Federal University of Minas Gerais

AIM To identify factors associated with depressive symptoms among inpatients with cardiovascular disease(CVD). METHODS This is a cross-sectional study performed in a subsample of a large cross-sectional research that investigated affective disorders and suicide behaviour among inpatients hospitalized in non-surgical wards of the University Hospital of the Federal University of Minas Gerais from november 2013 to October 2015. Sociodemographic and clinical data were obtained through a structured interview and medical record review. Depression was assessed by the depression subscale of the Hospital Anxiety and Depression Scale, with scores ≥ 8 considered as positive screening for depression. We used the Fagestr?m Test for nicotine Dependence to characterize nicotine dependence. For assessing resilience and early-life trauma, we used the raw scores of the Wagnild and Young Resilience Scale and Childhood Trauma Questionnaire, *** At endpoint, we included 137 subjects. Thirty-eight(27.7%) subjects presented depressive symptoms and nine(23.7%) of those were receiving antidepressant treatment during hospitalization. The female sex; a lower mean educational level; a greater prevalence of previous suicide attempts; a higher level of pain; a higher prevalence of family antecedents of mental disorders; a lower resilience score; and higher childhood trauma score were the factors significantly associated with screening positive for major depression(P nalysis demonstrated that the factors independently associated with the depressive symptoms were a higher childhood trauma severity(OR = 1.06; P = 0.004); moderate to severe nicotine dependence(OR = 8.58; P = 0.008); and the number of previous hospital admissions(OR = 1.11; P = 0.034). The obtained logistic model was considered valid, indicating that the three factors together distinguished between having or not depressive symptoms, and correctly classified 74.6% of individuals in the ***

关键词： Inpatients Depression Cardiovascular disease Depressive symptoms General hospital

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