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Bacteroides fragilis Supernatant Extracts Enriched in Phenylacetic Acid Induce a Cytotoxic Effect in Mammalian Cells

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Advances in Microbiology 2015年第10期5卷 730-736页

作者： Laís S.Falcao Eduardo n.F.Antunes Eliane O.Ferreira Heidi Pauer Maria Teresa V.Romanos Rossiane C.Vommaro Sérgio H.Seabra Daniela S.Alviano Celuta S.Alviano Antonio Jorge R.da Silva Leandro A.Lobo Regina Maria C.P.Domingues departamento de Ciências Básicas Pólo Universitário de Nova FriburgoUniversidade Federal FluminenseNova FriburgoBrasil Instituto de Microbiologia Prof.Paulo de Góes Universidade Federal do Rio de JaneiroRio de JaneiroBrasil Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de JaneiroRio de JaneiroBrasil Centro Universitário estadual da Zona Oeste—UEZO Rio de JaneiroBrasil Instituto de Pesquisa Produtos naturais Universidade Federal do Rio de JaneiroRio de JaneiroBrasil

Bacteroides species are nearly half of the fecal flora community and some are host symbionts crucial to host nutrition and systemic immunity. Among Bacteroides species B. fragilis strains are considered to be the opportunistic ones, being the most isolated anaerobic bacteria in clinical samples. Cell-free supernatants of 65 B. fragilis strains were assayed and they were capable of inducing vacuolating phenotype on Vero cells lineage. The supernatant of the Bacteroides fragilis ATCC 23745 strain was elicited to have the strongest vacuolating effect on Vero cells monolayers and peritoneal macrophages. Some drastic cell alterations were observed, such as a general disorganization of cytoplasm and chromatin condensation, evidencing cell death. By transmission electron microscopy it was confirmed that the vacuoles observed were, in fact, swollen mitochondria. An immunocytochemical assay, TUnEL, was used to confirm this hypothesis and showed that Vero cells and peritoneal macrophages were dying by apoptotic process after exposition of B. fragilis cell-free supernatant. Physical analysis of the apoptotic factor has revealed properties similar to short-chain fatty acids. After gas chromatography and mass spectrometry analysis, phenylacetic acid (PA) was characterized as the major compound present in the most purified active fraction. We believe that the PA is responsible for the pro-apoptotic effect elicited by the supernatant of B. fragilis cultures.

关键词： Bacteroides fragilis Vacuolization Apoptosis Vero cells Lineage Peritoneal Macrophages Phenylacetic Acid

内镜和组织学检查在胃萎缩程度评定中的一致性

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世界核心医学期刊文摘（胃肠病学分册） 2005年第8期1卷 55-55页

作者： Uemura n 杨雪娟

Background. This study was conducted to investigate the strength of agreement between the endoscopic atrophic border(EAB) and the histological score for atrop hy. Methods.A series of 298 dyspeptic Japanese patients underwent upper endoscop y. The grade of gastric atrophy was estimated according to the EAB. Antral and c orpus biopsy specimens were taken and were evaluated semiquantitatively accordin g to the updated Sydney classification system, including the score for atrophy. The StatExact software package was used to calculate the weighted kappa statisti cs. Results. The strength of agreement between the endoscopic atrophy score (EAB ) and the histological atrophy score was good, with a weighted kappa value of 0. 51 (95%confidence interval, 0.44-0.59). Conclusions.T- he strength of agreement between the end oscopic and histological atrophy scor es is not worse than the interobserver histological agreement between two pathol ogists. It is worthwhile to carry out further research on the use of the EAB to identify and score gastric atrophy.

关键词：组织学检查程度评定 Sydney 胃体部半定量测定统计值分类系统

北方旱寒区冬油菜不同后茬作物根际与非根际土壤肥力研究

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干旱地区农业研究 2015年第3期33卷 55-61页

作者：杨刚孙万仓王丽萍慕平刘自刚李学才杨宁宁孔德晶鲁美宏甘肃省油菜工程技术研究中心甘肃兰州730070 甘肃省干旱生境作物重点实验室/甘肃省作物遗传改良与种质创新重点实验室甘肃兰州730070 甘肃农业大学农学院甘肃兰州730070

以脲酶、过氧化氢酶、蔗糖酶和磷酸酶4种土壤酶活性及土壤理化性状为指标,研究了冬油菜后茬作物油菜、胡麻、玉米和小麦的根际与非根际土壤肥力状况。结果表明,4种作物的根际与非根际土壤肥力差异较大。根际土壤的酶活性与理化性状均显... 详细信息

以脲酶、过氧化氢酶、蔗糖酶和磷酸酶4种土壤酶活性及土壤理化性状为指标,研究了冬油菜后茬作物油菜、胡麻、玉米和小麦的根际与非根际土壤肥力状况。结果表明,4种作物的根际与非根际土壤肥力差异较大。根际土壤的酶活性与理化性状均显著高于休闲对照,而非根际土壤只有脲酶、有机质含量和速效氮等优于休闲对照。相关性分析表明土壤酶与理化性状之间相关性较大,它们都是评价土壤肥力的重要指标。利用隶属函数法将各土壤肥力分为3级,各处理肥力状况依次为:小麦根际(珔F=0.72)>胡麻根际(珔F=0.61)>油菜根际(珔F=0.56)>玉米非根际(珔F=0.44)>胡麻非根际(珔F=0.39)>小麦非根际(珔F=0.38)>玉米根际(珔F=0.37)>油菜非根际(珔F=0.29)>休闲CK(珔F=0.15)。分析结果表明各项指标采用隶属函数法综合评价冬油菜不同后茬作物根际与非根际土壤肥力是可行的。

关键词：冬油菜后茬作物土壤肥力根际与非根际土壤

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颗粒活性炭过滤器的室内空气净化效果

过滤与分离 1999年第3期9卷 22-24页

作者：戴飞汤广发李念平涂卫中莫仕虎严志宇湖南大学环境工程系湖南长沙410082 顺风空调有限公司湖南湘潭411000

论述了在空调房间内空气品质日益恶化的问题，通过对一台装有活性炭过滤器的吊顶风柜进行多项性能测试，结果表明。

关键词：室内空气品质活性炭吸附过滤器

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海绵窦型硬脑膜动静脉瘘

中华神经医学杂志 2005年第5期4卷 462-464页

作者：杨铭马廉亭薛德麟雷霆余泽姚国杰广州军区武汉总医院神经外科武汉430070 华中科技大学同济医学院附属同济医院神经外科武汉430030

目的探讨海绵窦型硬脑膜动静脉瘘的最佳检查与治疗方法。方法对比分析34例该病患者各种检查及血管内栓塞治疗的结果。结果CT检查阳性9例;MRI、MRA检查阳性表现23例;3D-TCD检查有24例表现患侧眼静脉流速升高;Dsa检查可以清楚地反应海绵... 详细信息

目的探讨海绵窦型硬脑膜动静脉瘘的最佳检查与治疗方法。方法对比分析34例该病患者各种检查及血管内栓塞治疗的结果。结果CT检查阳性9例;MRI、MRA检查阳性表现23例;3D-TCD检查有24例表现患侧眼静脉流速升高;Dsa检查可以清楚地反应海绵窦型硬脑膜动静脉瘘的全貌。34例患者有17例获解剖学治愈,4例临床治愈,10例好转,1例出现偏瘫,2例未治。结论Dsa是确诊海绵窦型硬脑膜动静脉瘘的金标准,能显示出瘘口的类型、血供及静脉引流方式;CT、MRI、MRA及3D-TCD只能反应部分颅内继发性改变。血管内栓塞是目前治疗海绵窦型硬脑膜动静脉瘘唯一安全有效的方法。

关键词：硬脑膜动静脉瘘海绵窦诊断显像栓塞,治疗性

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脉络膜恶性黑色素瘤的CT诊断

医学影像学杂志 2003年第8期13卷 562-563页

作者：李祖瑞巩若箴邢德海晁宝婷山东省临清市人民医院CT室山东临清252600 山东省医学影像学研究所山东济南250021 山东省临清市中医院放射科山东临清252600

目的 :探讨脉络膜恶性黑色素瘤的CT诊断价值。方法 :回顾性分析 2 7例经手术病理证实的脉络膜恶性黑色素瘤的CT表现。结果 :脉络膜恶性黑色素瘤表现为宽基底扁平状 7例 ,半球状及蘑菇状突向玻璃体腔内 2 0例 ,3例有囊性变 ,全部病例均... 详细信息

目的 :探讨脉络膜恶性黑色素瘤的CT诊断价值。方法 :回顾性分析 2 7例经手术病理证实的脉络膜恶性黑色素瘤的CT表现。结果 :脉络膜恶性黑色素瘤表现为宽基底扁平状 7例 ,半球状及蘑菇状突向玻璃体腔内 2 0例 ,3例有囊性变 ,全部病例均未见钙化 ,合并视网膜脱离 1 2例。结论 :CT可准确的确定肿瘤的位置、大小、形态、密度、有无球外侵犯、有无合并视网膜脱离 ,对指导制定治疗方案。

关键词：恶性黑色素瘤脉络膜体层摄影术 X线计算机

大玉竹低聚糖硫酸酯抗HSV-2病毒活性的研究

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应用与环境生物学报 2000年第5期6卷 483-486页

作者：杨敏蒙义文中国科学院成都生物研究所成都610041

从传统中药大玉竹中提取低聚糖Poos ,用吡啶氯磺酸法制备了它的硫酸酯衍生物S Poos.产物经红外鉴定 ,证实Poos硫酸酯化后羟基连上了硫酸基 ,硫含量为 8.47% ,取代度 (Ds)为 0 .6 .体外实验研究了Poos、S Poos对非洲绿猴肾细胞 (Vero)... 详细信息

从传统中药大玉竹中提取低聚糖Poos ,用吡啶氯磺酸法制备了它的硫酸酯衍生物S Poos.产物经红外鉴定 ,证实Poos硫酸酯化后羟基连上了硫酸基 ,硫含量为 8.47% ,取代度 (Ds)为 0 .6 .体外实验研究了Poos、S Poos对非洲绿猴肾细胞 (Vero)的细胞毒性和抗单纯疱疹病毒 2型 (HSV 2 )的活性 .结果表明 ,Poos和S Poos都无细胞毒性 .Poos本身无抗病毒活性 ,对HSV 2引起的细胞病变和空斑形成都无抑制作用 ;经硫酸酯衍生后的S Poos获得抗病毒活性 ,12 5～ 2 0 0 0 μg/mL的S Poos能很好地抑制HSV 2引起的细胞病变 ,2 0 0 μg/mL的S Poos能完全抑制病毒空斑形成 .图 4表 2参

关键词：大玉竹低聚糖硫酸酯单纯疱疹病毒抗病毒活性

Fungal diversity notes 1512–1610: taxonomic and phylogenetic contributions on genera and species of fungal taxa

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Fungal Diversity 2022年第6期117卷 1-272页

作者： Ruvishika S.Jayawardena Kevin D.Hyde Song Wang Ya‑Ru Sun nakarin Suwannarach Phongeun Sysouphanthong Mohamed A.Abdel‑Wahab Faten A.Abdel‑Aziz Pranami D.Abeywickrama Vanessa P.Abreu Alireza Armand AndréAptroot Dan‑Feng Bao Dominik Begerow Jean‑Michel Bellanger Jadson D.P.Bezerra Digvijayini Bundhun Mark S.Calabon Ting Cao Taimy Cantillo João LVRCarvalho napalai Chaiwan Che‑Chih Chen Régis Courtecuisse Bao‑Kai Cui Ulrike Damm Cvetomir M.denchev Teodor T.denchev Chun Y.deng Bandarupalli devadatha nimali Ide Silva Lidiane Ados santos nawal K.Dubey Sylvain Dumez Himashi SFerdinandez André L.Firmino Yusufon Gaforov Achala J.Gajanayake deecksha Gomdola Sugantha Gunaseelan Shucheng‑He Zin H.Htet Malarvizhi Kaliyaperumal Martin Kemler Kezhocuyi Kezo nuwan DKularathnage Marco Leonardi Ji‑Peng Li Chunfang Liao Shun Liu Michael Loizides Thatsanee Luangharn Jian Ma Hugo Madrid S.Mahadevakumar sajeewa S.n.Maharachchikumbura Dimuthu S.Manamgoda María P.Martín niranjan Mekala Pierre‑Arthur Moreau Yan‑Hong Mu Pasouvang Pahoua Dhandevi Pem Olinto L.Pereira Wiphawanee Phonrob Chayanard Phukhamsakda Mubashar Raza Guang‑Cong Ren Andrea C.Rinaldi Walter Rossi Binu C.samarakoon Milan Csamarakoon Vemuri V.sarma Indunil C.Senanayake Archana Singh Maria F.Souza Cristina M.Souza‑Motta Adriano A.Spielmann Wenxin Su Xia Tang XingGuo Tian Kasun M.Thambugala naritsada Thongklang Danushka S.Tennakoon nopparat Wannathes DingPeng Wei Stéphane Welti Subodini n.Wijesinghe Hongde Yang Yunhui Yang Hai‑Sheng Yuan Huang Zhang Jingyi Zhang Abhaya Balasuriya Chitrabhanu SBhunjun Timur S.Bulgakov Lei Cai Erio Camporesi Putarak Chomnunti Y.S.deepika Mingkwan Doilom Wei‑Jun Duan Shi‑Ling Han naruemon Huanraluek EBGareth Jones nLakshmidevi Yu Li saisamorn Lumyong Zong‑Long Luo Surapong Khuna Jaturong Kumla Ishara S.Manawasinghe Ausana Mapook Wilawan Punyaboon saowaluck Tibpromma Yong‑Zhong Lu JiYe Yan Yong Wang Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Innovative Institute for Plant Health Zhongkai University of Agriculture and EngineeringGuangzhou 510225P.R.China department of Plant Pathology College of AgricultureGuizhou UniversityGuiyang 550025GuizhouChina Research Center of Microbial Diversity and Sustainable Utilization Chiang Mai UniversityChiang Mai 50200Thailand department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Biotechnology and Ecology Institute Ministry of Agriculture and ForestryP.O.Box:811Vientiane CapitalLao PDR department of Botany and Microbiology Faculty of ScienceSohag UniversitySohag 82524Egypt Beijing Key Laboratory of Environment-friendly Management on fruit Diseases and Pests in north China Institute of Plant ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China departamento de Fitopatologia Universidade Federal de ViçosaViçosaMinas GeraisBrazil Laboratório de Botânica/Liquenologia Instituto de BiociênciasUniversidade Federal de Mato Grosso do SulAvenida Costa e Silva S/NBairro UniversitárioCampo GrandeMato Grosso do Sul CEP 79070-900Brazil College of Agriculture and Biological Sciences Dali UniversityDali 671003YunnanChina department of Entomology and Plant Pathology Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand Institute of Plant Science and Microbiology Universität HamburgOrganismic Botany and MycologyOhnhorststr.1822609 HamburgGermany CEFE CNRSUniv.MontpellierEPHEIRDINSERM1919Route de Mende34293 Montpellier Cedex 5France Setor de Micologia Departamento de Biociências e TecnologiaInstituto de Patologia Tropical e Saúde PúblicaUniversidade Federal de GoiásRua 235S/NSetor UniversitárioGoiâniaGO CEP:74605-050Brazil Division of Biological Sciences College of Arts and SciencesUniversity of the Philippines Visayas5023 MiagaoIloiloPhilippines CA

This article is the 14th in the Fungal Diversity notes series,wherein we report 98 taxa distributed in two phyla,seven classes,26 orders and 50 families which are described and *** in this study were collected from Australia,Brazil,Burkina Faso,Chile,China,Cyprus,Egypt,france,french Guiana,India,Indonesia,Italy,Laos,Mexico,Russia,Sri Lanka,Thailand,and *** are 59 new taxa,39 new hosts and new geographical distributions with one new *** 59 new species comprise Angustimassarina kunmingense,Asterina lopi,Asterina brigadeirensis,Bartalinia bidenticola,Bartalinia caryotae,Buellia pruinocalcarea,Coltricia insularis,Colletotrichum fexuosum,Colletotrichum thasutense,Coniochaeta caraganae,Coniothyrium yuccicola,dematipyriforma aquatic,dematipyriforma globispora,dematipyriforma nilotica,Distoseptispora bambusicola,Fulvifomes jawadhuvensis,Fulvifomes malaiyanurensis,Fulvifomes thiruvannamalaiensis,Fusarium purpurea,Gerronema atrovirens,Gerronema favum,Gerronema keralense,Gerronema kuruvense,Grammothele taiwanensis,Hongkongmyces changchunensis,Hypoxylon inaequale,Kirschsteiniothelia acutisporum,Kirschsteiniothelia crustaceum,Kirschsteiniothelia extensum,Kirschsteiniothelia septemseptatum,Kirschsteiniothelia spatiosum,Lecanora immersocalcarea,Lepiota subthailandica,Lindgomyces guizhouensis,Marthe asmius pallidoaurantiacus,Marasmius tangerinus,neovaginatispora mangiferae,Pararamichloridium aquisubtropicum,Pestalotiopsis piraubensis,Phacidium chinaum,Phaeoisaria goiasensis,Phaeoseptum thailandicum,Pleurothecium aquisubtropicum,Pseudocercospora vernoniae,Pyrenophora verruculosa,Rhachomyces cruralis,Rhachomyces hyperommae,Rhachomyces magrinii,Rhachomyces platyprosophi,Rhizomarasmius cunninghamietorum,Skeletocutis cangshanensis,Skeletocutis subchrysella,Sporisorium anadelphiae-leptocomae,Tetraploa dashaoensis,Tomentella exiguelata,Tomentella fuscoaraneosa,Tricholomopsis lechatii,Vaginatispora favispora and Wetmoreana *** new combin

关键词： 59 new taxa 39 new records Ascomycota Basidiomycota Dothideomycetes Lecanoromycetes Leotiomycetes Sordariomycetes Ustilaginomycotina

Relationship between perioperative anaemia and outcomes in older people with hip fractures: A systematic review and meta-analysis protocol

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World Journal of Meta-Analysis 2019年第6期7卷 290-296页

作者： Kareeann SF Khow Michelle WK Kee Pazhvoor n Shibu Solomon CY Yu Mellick J Chehade Renuka Visvanathan Aged and Extended Care Services the Queen Elizabeth HospitalWoodville SouthSA 5011Australia Centre of Research Excellence Frailty Trans-disciplinary Research to Achieve Healthy AgeingUniversity of AdelaideAdelaideSA 5005Australia Discipline of Orthopaedics and Trauma Faculty of Medical and Health SciencesUniversity of AdelaideAdelaideSA 5005Australia

BACKGROUnD Hip fractures are common with increasing age and is associated with decline in mobility. Both the fracture and the surgery can lead to blood loss, resulting in anaemia. However, it is uncertain at which time point haemoglobin is most strongly associated with different clinical outcomes after hip fracture. Our hypothesis is perioperative anaemia (admission, postoperative and discharge) during hip fracture surgery is associated with poor clinical outcomes. AIM To determine the effects of perioperative anaemia during hip fracture surgery on mortality, functional status and other clinical outcomes. METHODS Electronic databases will be searched to identify studies evaluating perioperative anaemia and outcomes of hip fracture surgery. Reference lists of included studies will also be searched to identify additional published studies. Eligibility criteria are as follows: Population: People who underwent hip fracture surgery;Exposure: Perioperative anaemia;Comparison: no anaemia before or after hip fracture surgery;Outcome: Mortality, hospital length of stay, postoperative complications, hospital readmission, change of discharge destination, quality of life and functional status. Risk of bias assessment will be assessed using the Cochrane Collaboration’s tool for randomized controlled trials and the modified version of the Epidemiological Appraisal Instrument for observational studies. Data will be pooled for meta-analysis if deemed appropriate. COnCLUSIOn This review seeks to clarify outcomes associated with perioperative anaemia at various time-points around hip fracture surgery. These findings will potentially inform evidence-based clinical practice on interventions in those with anaemia.

关键词： Anaemia Haemoglobin Hip fracture Length of stay Mortality Outcomes Perioperative Readmission