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Pectate lyase is a factor in the adaptability for Heterodera glycines infecting tobacco

Journal of Integrative Agriculture 2019年第3期18卷 618-626页

作者： TIAN Zhong-ling SHI Hong-li munawar maria ZHENG Jing-wu Laboratory of Plant Nematology Institute of BiotechnologyCollege of Agriculture&BiotechnologyZhejiang University School of Resource and Environmental Sciences Henan Institute of Science and Technology Key Lab of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture

The soybean cyst nematode, Heterodeara glycines, is a serious pathogen of soybean, and reported to be the host of a wide range of Fabaceae. In the present study, the host specificity and reproductivity of two populations of H. glycines collected from soybean and tobacco were identified and characterized. The comparative identity between β-1,4-endoglucanase, pectate lyase and chorismate mutase of H. glycines parasitizing on soybean and tobacco were 99, 97 and 98%, respectively. The qR T-PCR analysis indicated that the expression of pectate lyase 2 gene was significantly higher in second-stage juveniles of H. glycines Henan population parasitizing on tobacco than that of H. glycines Shanxi population parasitizing on soybean. In addition, the pectic acid content of cell wall was significantly higher(45%) in the roots of tobacco than the roots of soybean. Our results indicate that the changes in transcript parasitism genes may be a result of long-term evolution illustrating how a plant-parasitic nematode adapts to the host environment for optimal infestation and survival.

关键词： s: Heterodera glycines tobacco parasitism genes β-1,4-endoglucanase pectate lyase chorismate mutase pectic acid

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Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China

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Journal of Integrative Agriculture 2018年第12期17卷 2724-2733页

作者： TIAN Zhong-ling munawar maria Eda Marie Barsalote Pablo Castillo ZHENG Jing-wu Laboratory of Plant Nematology Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University Institute for Sustainable Agriculture(IAS) Spanish National Research Council (CSIC) Campus de Excelencia Internacional Agroalimentario Key Lab of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture

The rice root-knot nematode Meloidogyne graminicola is a severe pest of rice. In China, it was first reported from Hainan Province, and later from several other provinces. In the present study, a rice root-knot nematode population found from the rice cultivation areas of Zhejiang Province, China is characterized via molecular analysis using internal transcribed spacer(ITS) and cytochrome c oxidase subunit Ⅱ(coxⅡ)-16 S rRNA genes and scanning electron microscopy(SEM) observations of males and the second-stage juveniles. Morphometric data and molecular sequence comparisons for all M. graminicola populations occurring in China are also provided. The overall morphology of M. graminicola found in Zhejiang match well with the original description, though males have a slightly longer body and stylet, and a shorter tail, while the second-stage juvenile is also slightly longer than in the original description. This is the first report of M. graminicola from Zhejiang. Phylogenetic studies based on coxⅡ suggest that all the Chinese populations belong to Type B. This study expands knowledge of the increasing distribution and phylogenetic relationships of M. graminicola that occur in China.

关键词： Meloidogyne graminicola morphology morphometric molecular rice rDNA cytochrome c oxidase subunit Ⅱ (coxⅡ) China

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Diagnosis and characterization of the ribosomal DNA-ITS of potato rot nematode(Ditylenchus destructor) populations from Chinese medicinal herbs

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Journal of Integrative Agriculture 2023年第6期22卷 1763-1781页

作者： NI Chun-hui HAN Bian LIU Yong-gang maria munawar LIU Shi-ming LI Wen-hao SHI Ming-ming LI Hui-xia PENG De-liang College of Plant Protection Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu ProvinceLanzhou 730070P.R.China Institute of Plant Protection Gansu Academy of Agricultural SciencesLanzhou 730070P.R.China Department of Biological Sciences University of LethbridgeLethbridge 4401Canada State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

The potato rot nematode(Ditylenchus destructor) is a very economically important nematode in agronomic and horticultural plants worldwide. In this study, 43 populations of D. destructor were collected from different hosts across China, including 37 populations from Chinese herbal medicine plants. Obtained sequences of ITS-rDNA and D2–D3of 28S-rDNA genes of D. destructor were compared and analyzed. Nine types of significant length variations in ITS sequences were observed among all populations. The differences in ITS1 length were mainly caused by the presence of repetitive elements with substantial base substitutions. Reconstructions of ITS1 secondary structures showed that the minisatellites formed a stem structure. Ten haplotypes were observed in all populations based on mutations and variations of helix H9. Among them, 3 known haplotypes(A–C) were found in 7 populations isolated from potato,sweet potato, and Codonopsis pilosula, and 7 unique haplotypes were found in other 36 populations collected from C. pilosula and Angelica sinensis compared with 7 haplotypes(A–G) according to Subbotin' system. These unique haplotypes were different from haplotypes A–G, and we named them as haplotypes H–N. The present results showed that a total of 14 haplotypes(A–N) of ITS-rDNA have been found in D. destructor. Phylogenetic analyses of ITSrDNA and D2–D3 showed that all populations of D. destructor were clustered into two major clades: one clade only containing haplotype A from sweet potato and the other containing haplotypes B–N from other plants. For further verification, PCR-ITS-RFLP profiles were conducted on 7 new haplotypes. Collectively, our study suggests that D. destructor populations on Chinese medicinal materials are very different from those on other hosts and this work provides a paradigm for relevant researches.

关键词： Ditylenchus destructor minisatellites ITS-RFLP phylogeny RNA secondary structure

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Characterization and diagnostics of stubby root nematode Trichodorus cedarus Yokoo,1964(Dorylaimida:Trichodoridae)from Zhejiang Province,China

Characterization and diagnostics of stubby root nematode Tri...

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中国植物病理学会2017年学术年会

作者： Barsalote Eda Marie munawar maria Tian Zhongling Cai Ruihang Li Xiaolin Qu Nan 郑经武 Laboratory of Plant Nematology Institute of BiotechnologyCollege of Agriculture &BiotechnologyZhejiang University

The study was conducted to characterize morphological and molecular profiles of seven isolates of Trichodorus cedarus recovered from deciduous trees and ornamentals in Zhejiang Province,Eastern *** of species was derived from polytomous key using prime character codes:E,G,H and subgroup codes:J,K,L,M for male populations while prime character codes:D,C,L,K and subgroup codes:G,H,J for female *** is characterized by a short body(0.63.90 mm),onchiostyle 559μm and secretory-excretory pore(S-E) situated along the anterior 2/3 of pharyngeal *** females,*** is distinguished by possessing didelphic genital system,transverse slit vulva located 52%60% along the body,vaginal refractive pieces approximately 1 1.9μm size,vagina pear-shaped withtriangular to rounded triangular vaginal *** males,*** is characterized by having 3 ventromedian cervical papillae(CP) located between onchiostyle base and S-E pore,presence of 3 ventromedian precloacal supplements with posterior supplement(SP 1)at level of retracted spicule,large sperms with sausage-shaped nucleus,spicule short(38.1 45.4 pμm)appearing slightly curved or nearly straight,manubrium offset without capitular extension and shaft more or less cylindrical with continuous fine ornamentations except at *** codes for identification of species are:D1-C1-L1-K2-A12-B23-E3-F1-G2-Hl-I2-J1-M1-N1-O1-P1-Q2-R1 in females and F34-D3-P2-A21-B23-C21-E0-G1-H3-I1-J21-K32-L2-M2-N1-O5 in *** based on partial 18 S,ITS1 and D2-D3 segments of 28 S obtained a fragment length of 1 726 743 bp,1 221 225 bp and 79699 bp,*** results revealed 99% sequence identity to *** ZJ8(HM106502).Similarly,phylogenetic reconstruction showed cluster of present isolates to *** putative species from Zhejiang with 99% clade *** paper takes into account the morphological and molecular comparisons among intraspecies of ***,the most prevalent tric

关键词： Stubby root nematode Morphology rDNA China

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Ultrastructure,Morphological and Molecular Characterization of New and New Recoreded Species of Hemicriconemoides from China

Ultrastructure,Morphological and Molecular Characterization ...

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中国植物病理学会2017年学术年会

作者： maria munawar Tian Zhongling Eda Marie Barsalote Cai Ruihang Li Xiaolin Qu Nan 郑经武 Laboratory of Plant Nematology Institute of BiotechnologyCollege of Agriculture &BiotechnologyZhejiang University

Sheathoid nematodes of the genus Hemicriconemoides are migratory root-ectoparasites of many plants including various agricultural crops and fruit *** are generally found inhabiting tropical and subtropical areas of the world and have been reported from Africa,America,Asia,Australia and southern Europe,presently genus consists of 52 valid *** this study we provide morphological and molecular characterization of one new and one new recorded species *** *** *** isolated from Metasequoia glyptostroboides and Camelliae uruku in Hangzhou,Zhejiang,*** new species is most similar to *** and considered as a cryptic *** the new species can be characterized by cuticular sheath well separated at anterior region,cuticle smooth with retrorse annuli,lip region slightly set off and roughly square in ***733μm long,excretory pore 4 annuli posterior to pharyngeal bulb,vulva posteriorly located88%91%of the total body,tail conical with bluntly pointed *** can be characterized by its exclusive lip morphology(set off,marked with constriction),cuticular sheath attached at anterior and vulval region,stylet 668μm long,excretory pore located 4 5 annuli posterior to pharyngeal bulb,vulva posteriorly located 88%0%of the body,tail elongated widely conoid,ended up in a rounded *** electron microscopic observations and molecular characterization based on COI of mtDNA,18 S,D2-D3 28 S and ITS of rDNA sequences for both species also *** date sixteen Hemicriconemoides species have been reported from China,where *** is the 17 recorded *** results suggested that the concomitant use of morphological and molecular characterization help to remove the taxonomic opaqueness and paucity of knowledge regarding species *** will help to improve its relevance for posterity and will further elucidate the deeper levels of phylogenetic relationship

关键词： Hemicriconemoides Morphology Ultrastructure rDNA China

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Effectiveness of aerobic pretreatment of municipal solid waste for accelerating biogas generation during simulated landfilling

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Frontiers of Environmental Science & Engineering 2018年第3期12卷 79-87页

作者： munawar Ali Junli Zhang Roberto Raga maria Cristina Lavagnolo Alberto Pivato Xu Wang Yuanyuan Zhang Raffaello Cossu Dongbei Yue School of Environment Tsinghua University Beijing 100084 China Key Laboratory for Solid Waste Management and Environment Safety (Tsinghua University) Ministry of Education of China Beijing 100084 China Solid waste and Chemical Management Centre Ministry of Environmental Protection Beijing 100029 China DII Department of Industrial Engineering University of Padova Via Marzolo 9 35131 Padova Italy

This study evaluates the effectiveness of aerobic pretreatment of municipal solid waste （MSW） on reducing lag phase and accelerating biogas generation. Aerobic pretreatment degree （APD） was determined on the basis of reduction in volatile solids （VS） on a wet weight basis. In this study, intermittent aeration （IA） was applied to three reactors as a main aeration mode; since a single reactor was operated under continuous aeration mode. However, the purpose of the experiment was to reduce VS content of waste, irrespective of the comparison between aeration modes. Fresh MSW was first pretreated aerobically with different aeration rates （10, 40, 60 and 85 L/min/m3） for the period of 30- 50 days, resulting in VS-loss equivalent to 20%, 27%, 38% and 53q4 on w/w basis for the wastes AI, A2, A3 and A4, respectively. The cumulative biogas production, calculated based on the modified Gompertz model were 384, 195, 353,215, and 114 L/kg VS for the wastes A0, A1, A2, A3 and A4, respectively. Untreated waste （A0） showed a long lag phase; whereas the lag phases of pretreated MSW were reduced by more than 90e/L Aerobically pretreated wastes reached stable methanogenic phase within 41 days compared to 418 days for untreated waste. The waste mass decreased by about 8% to 27% compared to untreated MSW, indicative that even more MSW could be placed in the same landfill. The study confirmed the effectiveness of aerobic pretreatment of MSW prior to landfilling on reducing lag phase and accelerating biogas generation.

关键词： Municipal solid waste (MSW)Aerobic pretreatment degree (APD)Volatile solids (VS)Intermittent aeration (IA)Landfill gasLandfilling

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CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed Xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves Weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai Yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-Jun Bi Yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia Hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai Yuchen Cai Haiying Cai Huacheng Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang Wei Chao Auttakit Chatrabhuti Yimin Che Yuzhi Che Bin Chen Danping Chen Fuqing Chen Fusan Chen Gang Chen Guoming Chen Hua-Xing Chen Huirun Chen Jinhui Chen Ji-Yuan Chen Kai Chen Mali Chen Mingjun Chen Mingshui Chen Ning Chen Shanhong Chen Shanzhen Chen Shao-Long Chen Shaomin Chen Shiqiang Chen Tianlu Chen Wei Chen Xiang Chen Xiaoyu Chen Xin Chen Xun Chen Xurong Chen Ye Chen Ying Chen Yukai Chen Zelin Chen Zilin Chen Gang Chen Boping Chen Chunhui Chen Hok Chuen Cheng Huajie Cheng Shan Cheng Tongguang Cheng Yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi Ling-Yun Dai Xinchen Dai Xuwen Dai Antonio De maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De Sio Elisa Del Core Shuangxue Deng Wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding Siqin Dingl Yadong Ding Haiyi Dong Jianin AGH University of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui University HefeiAnhui Applied Physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu University Institute of ScienceVaranasi Bandirma Onyedi Eylul University Bandirma Beihang University Beijing Beijing Computational Science Research Center Beijing Beijing Conveyi LTD Beijing Beijing Glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal University Beijing Brown University ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad University Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo University Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central China Normal University WuhanHubei Central South University ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa Chengdu KaitengSifang Digital Radio&TV Equipment Co. LtdChengduSichuan China academy of engineering physics MianyangSichuan China building materials academy Beijing China Center of Advanced Science and Technology Beijing China Institute of Atomic Energy Beijing China Jiliang University HangzhouZhejiang China Nuclear Instrument CO. LTDBeijing China University of Geosciences Beijing China University of Geosciences WuhanHubei China University of Mining and Technology XuzhouJiangsu China West Normal University NanchongSichuan Chongqing University Department of PhysicsChongqing Chulalongkorn University Bangkok City University of Hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell University IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu University DalianLiaoning Dalian University of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East China University Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise

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