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The temporal-spatial expression and functional analysis of three gustatory receptor genes in Solenopsis invicta using sweet and bitter compounds

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Insect Science 2024年第2期31卷 448-468页

作者： muhammad irfan waris Yanyuan Lei Guojun Qi Ziying Guan Abdul Rashied Jie Chen Lihua Lyu Institute of Plant Protection Guangdong Academy of Agricultural SciencesGuangdong Provincial Key Laboratory of High Technology for Plant ProtectionGuangzhouChina Department of Entomology College of Plant ProtectionSouth China Agricultural UniversityGuangzhouChina

The insect gustatory system participates in identifying potential food sources and avoiding toxic *** this process,gustatory receptors(GRs)recognize feeding stimulant and deterrent ***,the GRs involved in recognizing stimulant and deterrent compounds in the red imported fire ant,Solenopsis invicta,remain ***,we conducted a study on the genes SinvGR1,SinvGR32b,and SinvGR28a to investigate the roles of GRs in detecting feeding stimulant and deterrent *** this current study,we found that sucrose and fructose are feeding stimulants and the bitter compound quinine is a feeding *** fire ant workers showed significant behavior changes to avoid the bitter taste in feeding stimulant *** transcription quantitative real-time polymerase chain reaction results from developmental stages showed that the SinvGR1,SinvGR32b,and SinvGR28a genes were highly expressed in fire ant ***-specific expression profiles indicated that SinvGR1,SinvGR32b,and SinvGR28a were specifically expressed in the antennae and foreleg tarsi of workers,whereas SinvGR32b gene transcripts were also highly accumulated in the male ***,the silencing of SinvGRI or SinvGR32b alone and the co-silencing of both genes disrupted worker stimulation and feeding on sucrose and *** results also showed that SinvGR28a is required for avoiding quinine,as workers with knockdown of the SinvGR28a gene failed to avoid and fed on *** study first identified stimulant and deterrent compounds of fire ant workers and then the GRs involved in the taste recognition of these *** study could provide potential target gustatory genes for the control of the fire ant.

关键词： behavioral responses feeding assays gustatory receptors RNA interference Solenopsis invicta temporal-spatial expression

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Appraisal of MsepCSP14 for chemosensory functions in Mythimna separata

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Insect Science 2022年第1期29卷 162-176页

作者： Aneela Younas muhammad irfan waris muhammad Shaaban muhammad Tahir ul Qamar Man-Qun Wang College of Plant Science and Technology Huazhong Agricultural UniversityWuhanChina Department of Soil Science Faculty of Agricultural Sciences and TechnologyBahauddin Zakariya UniversityMultanPakistan State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources College of Life Science and TechnologyGuangxi UniversityNanning530005China

Chemosensory proteins (CSPs) have great contributions in performing diverse functions in insects. However, physiological appraisal of chemosensory protein genes still remains elusive in insects. We studied expression patterns and binding affinities of MsepCSP14, a chemosensory protein, in Mythimna separata. The distinct functions of MsepCSP14 were validated by employing different molecular techniques. The MsepCSP14 had high resemblance of sequence with chemosensory proteins of other insect family members. The MsepCSP14 expression was higher in antennal tissues of females than other tissues. Fluorescence binding assay validated that binding of nine out of 21 ligands to MsepCSP14 was higher at pH 7.4 than at pH 5.0. Three dimensional modeling (3D) and docking analysis predicted that amino acid residues of MsepCSP14 were involved in binding of compounds, and behavior assay displayed that adults of M. separata considerably responded to four volatiles from compounds demonstrating strong binding ability to MsepCSP14. Results of the present study suggest that MsepCSP14 is likely to mediate chemosensory functions in M. separata.

关键词： chemoreception expression pattern fluorescent binding olfaction oriental army worm Y-tube

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Molecular and in vitro biochemical assessment of chemosensory protein 10 from brown planthopper Nilaparvata lugens at acidic pH

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Journal of Integrative Agriculture 2022年第3期21卷 781-796页

作者： muhammad irfan waris Aneela YOUNAS Rana muhammad Kaleem ULLAH Fatima RASOOL muhammad Muzammal ADEEL WANG Man-qun College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070P.R.China National Center for Bioinformatics Quaid-i-Azam UniversityIslamaba 45320Pakistan College of Informatics Huazhong Agricultural UniversityWuhan 430070P.R.China

Chemosensory proteins(CSPs)are important molecular components of the insect olfactory system,which are involved in capturing,binding,and transporting hydrophobic odour molecules across the sensillum in sensillar lymph in regulating insect *** protein family(CSPs)is also involved in many other systems that are not linked to olfactory receptors in olfactory *** brown planthopper(BPH)is a monophagous pest of rice that causes damage by sucking phloem sap and transmitting a number of diseases caused by *** this study,fluorescence competitive binding assay and fluorescence quenching assay at acidic p H were performed as well as homology modelling to describe the binding affinity of Nlug *** competitive binding assay(FCBA)demonstrated that Nlug CSP10 bound strongly to nonadecane,farnesene,and 2-tridecanone at acidic p *** results of FCBA indicated that Nlug CSP10 bound different ligands at the physiological p H(5.0)of the bulk sensillum *** quenching assay demonstrated that Nlug CSP10 generated a stable complex with 2-tridecanone,while two ligands nonadecane and farnesene collided due to molecular *** interaction of selected ligands with the modelled structure of Nlug CSP10 was also analyzed,which found the key amino acids(Gln23,Gln24,Gln25,Asn27,Met33,Ser34,Ile35,Tyr36,Asn42,Met43,Val45,Asn46,Asn93,Arg96,Ala97,Lys99,and Ala100)in Nlug CSP10 that were involved in binding of volatile *** present study contributes to the binding profile of Nlug CSP10 that promotes the development of behaviorally active ligands based on BPH olfactory system.

关键词： insect olfaction chemosensory protein Nilaparvata lugens fluorescence competitive binding assay fluorescence quenching assay molecular docking

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The role of chemosensory protein 10 in the detection of behaviorally active compounds in brown planthopper, Nilaparvata lugens

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Insect Science 2020年第3期27卷 531-544页

作者： muhammad irfan waris Aneela Younas muhammad Muzammal Adeel Shuang-Gang Duan Sundas Rana Quershi Rana muhammad Kaleem Ullah Man-Qun Wang Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina College of informatics Huazhong Agricultural UniversityWuhanChina

Chemosensory proteins(CSPs)play important roles in insects’chemoreception,although their specific functional roles have not been fully *** this study,we conducted the developmental expression patterns and competitive binding assay as well as knock‐down assay by RNA interference both in vitro and in vivo to reveal the function of NlugCSP10 from the brown planthopper(BPH),Nilaparvata lugens(Stål),a major pest in rice *** results showed that NlugCSP10 messenger RNA was significantly higher in males than in females and correlated to gender,development and wing *** fluorescence binding assays revealed that NlugCSP10 exhibited the highest binding affinity with cis‐3‐hexenyl acetate,eicosane,and(+)‐β‐*** assay revealed that eicosane displayed attractant activity,while cis‐3‐hexenyl acetate,similar to(+)‐β‐pinene significantly repelled *** *** of NlugCSP10,which is responsible for cis‐3‐hexenyl acetate binding,significantly disrupted cis‐3‐hexenyl acetate ***,findings of the present study showed that NlugCSP10 could selectively interrelate with numerous volatiles emitted from host plants and these ligands could be designated to develop slow‐release mediators that attract/repel *** and subsequently improve the exploration of plans to control this insect pest.

关键词： behavioral responses chemosensory proteins expression patterns fluorescence competitive binding assays RNA interference

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0.2%高效氯氰菊酯粉剂对田间红火蚁种群数量及蚁巢迁移的影响

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植物保护学报 2023年第5期50卷 1184-1192页

作者：齐国君王刚石庆型陆嘉进关子盈陈婷陈洁 muhammad irfan waris 吕利华广东省农业科学院植物保护研究所广东省植物保护新技术重点实验室广州510640 华南农业大学植物保护学院广州510642

为科学评价触杀性粉剂对红火蚁Solenopsis invicta Buren的防治效果,利用0.2%高效氯氰菊酯粉剂对田间红火蚁蚁巢进行单蚁巢处理,分析粉剂施用剂量和次数对田间红火蚁种群数量及蚁巢迁移的影响。结果表明,触杀性粉剂处理可在短期内显著... 详细信息

为科学评价触杀性粉剂对红火蚁Solenopsis invicta Buren的防治效果,利用0.2%高效氯氰菊酯粉剂对田间红火蚁蚁巢进行单蚁巢处理,分析粉剂施用剂量和次数对田间红火蚁种群数量及蚁巢迁移的影响。结果表明,触杀性粉剂处理可在短期内显著降低红火蚁的蚁巢数量和工蚁诱集数量,50 g/巢和100 g/巢处理1 d后,蚁巢数量降幅分别达55.17%和66.04%,工蚁诱集数量降幅分别达31.79%和51.95%,但5~9 d后基本恢复至处理前水平,第21天蚁巢增加率达86.21%和37.74%,持效性较差。增多粉剂施用次数同样也只能在短期内压低红火蚁的蚁巢数量和工蚁诱集数量,施用1、2和3次粉剂1 d后蚁巢数量降幅分别达61.44%、76.82%和80.79%,工蚁诱集数量降幅分别达55.40%、47.21%和28.36%,随着时间延长,蚁巢数量和工蚁数量很快恢复。虽然随着施药剂量增加,蚁巢增加率和分巢率明显降低,新蚁巢出现时间略有延迟,新蚁巢的体积明显减小,但整体来看蚁巢数量仍呈上升趋势,新蚁巢出现的距离和方位也没有明显差异。表明粉剂防治仅可在短期内表面上压低红火蚁种群数量,即使增加施药剂量和次数也不能有效降低红火蚁蚁巢数量,甚至会引起并加剧蚁巢的分巢扩散,建议在红火蚁防控中应慎重使用,或仅用于局部应急防控工作。

关键词：红火蚁粉剂种群数量分巢迁移

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The Influence of Depression on Different Age and Gender Groups in Bahawalpur

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Journal of Clinical and Nursing Research 2024年第7期8卷 196-207页

作者： Mehran Khan muhammad Shahbaz Tooba waris Shoaib Yaseen Iqra Afzal Emaan Khadim Sawera Usman muhammad Ismail Shah Sulaiman Aslam Zafar Iqbal Bahawalpur College of Pharmacy BMDC Complex63100PunjabPakistan

In Bahawalpur,a cross-sectional study assessed depression prevalence across age and gender groups,involving 442 participants from diverse socioeconomic backgrounds and *** the PHQ9 questionnaire,84%were found to meet depression *** findings revealed a higher incidence in females(88%)than males(79%),with the greatest disparity among young adults,particularly young women,due to factors like academic pressure and financial *** children had the lowest depression rates(68%),possibly due to better *** individuals exhibited more severe depression,likely related to aging and domestic *** study’s findings highlight a significant variation in depression severity across different demographic groups,with an overall higher incidence in *** research underscores the necessity for targeted mental health resources and interventions tailored to the specific needs of each demographic *** also points to the importance of addressing academic and socioeconomic stressors to mitigate depression,particularly among young *** the study provides valuable insights,it relies on self-reported data,which may introduce ***,future research should include clinical assessments to validate these findings and ensure a more accurate representation of depression within the community.

关键词： Influence Depression Different age Gender groups Bahawalpur

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MWCNTs/SWCNTs Nanofluid Thin Film Flow over a Nonlinear Extending Disc: OHAM Solution

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Journal of Thermal Science 2019年第1期28卷 115-122页

作者： GOHAR TAZA Gul waris Khan muhammad Shuaib muhammad Altaf Khan EBENEZER Bonyah Department of Mathematics University of Peshawar Department of Mathematics City University of Science and Information Technology Higher Education Department Khyber Pukhtunkhwa Department of Mathematics Islamia College Department of Mathematics Kumasi Techniical University

The aim of this research is the improvement towards the consumption of energy in the field of engineering and industry. The efforts have been paid to the enhancement of heat transmission and cooling process through a nanofluid coating of a nonlinear stretching disc. The combination of Water(H2 O) and multiple walled carbon nanotubes(MWCNT)/single walled carbon nanotubes(SWCNT) have been used as a nanofluid. The spreading of a thin nano-layer with variable thickness over a nonlinear and radially stretching surface has been considered. The estimated results of the problem have been accomplished using the Optimal Homotopy Analysis Method(OHAM). The residual errors of the OHAM method have been shown physically and numerically. The important physical parameters of skin friction and Nusselt number have been calculated and discussed. The other embedding parameters like generalized magnetic parameter, Prantl number, nanofluid volume fraction and Eckert number have been intended and discussed. The obtained results have been compared with the Numerical(ND-Solve) method for both sorts of CNTs. The closed agreement of both methods has been achieved.

关键词： CNTs-H2O based nanofluid variable thin layer nonlinear radially stretching disc magnetic field skin friction and Nusselt number OHAM & numerical method

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Hepatitis C virus NS5A promotes insulin resistance through IRS-1 serine phosphorylation and increased gluconeogenesis

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World Journal of Gastroenterology 2015年第43期21卷 12361-12369页

作者： Fahed Parvaiz Sobia Manzoor Jawed Iqbal Mehuli Sarkar-Dutta muhammad Imran Gulam waris Atta-urRahman School of Applied Biosciences National University of Sciences and Technology Department of Microbiology and Immunology Rosalind Franklin University of Medicine and Science

AIM: To investigate the mechanisms of insulin resistance in human hepatoma cells expressing hepatitis C virus(HCV) nonstructural protein 5A(NS5A).METHODS: The human hepatoma cell lines,Huh7 and Huh7.5,were infected with HCV or transientlytransfected with a vector expressing HCV NS5 A. The effect of HCV NS5 A on the status of the critical players involved in insulin signaling was analyzed using realtime quantitative polymerase chain reaction and Western blot assays. Data were analyzed using Graph Pad Prism version ***: To investigate the effect of insulin treatment on the players involved in insulin signaling pathway,we analyzed the status of insulin receptor substrate-1(IRS-1) phosphorylation in HCV infected cells or Huh7.5 cells transfected with an HCV NS5 A expression vector. Our results indicated that there was an increased phosphorylation of IRS-1(Ser307) in HCV infected or NS5 A transfected Huh7.5 cells compared to their respective controls. Furthermore,an increased phosphorylation of Akt(Ser473) was observed in HCV infected and NS5 A transfected cells compared to their mock infected cells. In contrast,we observed decreased phosphorylation of Akt Thr308 phosphorylation in HCV NS5 A transfected cells. These results suggest that Huh7.5 cells either infected with HCV or ectopically expressing HCV NS5 A alone have the potential to induce insulin resistance by the phosphorylation of IRS-1 at serine residue(Ser307) followed by decreased phosphorylation of Akt Thr308,Fox01 Ser256 and GSK3β Ser9,the downstream players of the insulin signalingpathway. Furthermore,increased expression of PECK and glucose-6-phosphatase,the molecules involved in gluconeogenesis,in HCV NS5 A transfected cells was ***: Taken together,our results suggest the role of HCV NS5 A in the induction of insulin resistance by modulating various cellular targets involved in the insulin signaling pathway.

关键词： Hepatitis C virus nonstructural protein 5A Insulin

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Optimized health care for subjects with type 1 diabetes in a resource constraint society: A three-year follow-up study from Pakistan

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World Journal of Diabetes 2019年第3期10卷 224-233页

作者： muhammad Yakoob Ahmedani Asher Fawwad Fariha Shaheen Bilal Tahir Nazish waris Abdul Basit Department of Medicine Baqai Institute of Diabetology and Endocrinology Baqai Medical University Karachi 74600 Pakistan Department of Biochemistry Baqai Medical University Research Department Baqai Institute of Diabetology and Endocrinology Baqai Medical University Karachi 74600 Pakistan Research Department Baqai Institute of Diabetology and Endocrinology Baqai Medical University Karachi 74600 Pakistan

BACKGROUND Inadequate health infrastructure and poverty especially in rural areas are the main hindrance in the optimal management of subjects with type 1 diabetes (T1D) in Pakistan. AIM To observe effectiveness of diabetes care through development of model clinics for subjects with T1D in the province of Sindh Pakistan. METHODS A welfare project with name of “Insulin My Life”, was started in province of Sindh, Pakistan. This was collaborative work of Baqai Institute of Diabetology and Endocrinology, World Diabetes Foundation and Baqai Medical University between February 2010 to February 2013. Under this project thirty-four T1D clinics were established. Electronic database was designed for demographic, biochemical, anthropometric and medical examination. Monthly consultation was part of the standardized diabetes care. All the recruited subjects with T1D were provided free insulins and related materials. RESULTS Out of 1428 subjects, 795 (55.7%) were males and 633 (44.3%) were females. Subjects were categorized into ≤ 5 years of age 103 (7.2%), between 6-12 years 323 (22.6%), between 13–18 years 428 (29.7%) and ≥ 19 years of age 574 (40.2%) groups. Glycemic control as assessed by HbA1c was significantly improved (P 0.05). CONCLUSION This study gives us long-term longitudinal data of people with T1D in a resource constraint society. With provision of standardized and comprehensive care significant improvement in glycemic control without any change in the frequency of microvascular complications was observed over 3 years.

关键词： Insulin My Life Type 1 diabetes Insulin Care Pakistan

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A Transfer Learning Based Approach for COVID-19 Detection Using Inception-v4 Model

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Intelligent Automation & Soft Computing 2023年第2期35卷 1721-1736页

作者： Ali Alqahtani Shumaila Akram muhammad Ramzan Fouzia Nawaz Hikmat Ullah Khan Essa Alhashlan Samar MAlqhtani Areeba waris Zain Ali College of Computer Science and Information Systems Najran UniversityNajran61441Saudi Arabia Department of Computer Science and Information Technology University of SargodhaSargodha40100Pakistan School of Systems and Technology University of Management and TechnologyLahore54782Pakistan Department of Computer Science COMSATS University IslamabadWah CampusIslamabad47040Pakistan Department of Medical Imaging King Khaled HospitalNajran61441Saudi Arabia Electrical&Electronics Engineering Department Universiti TeknologiPetronas31750Malaysia Department of Electrical Engineering HITEC UniversityTaxila47080Pakistan

Coronavirus(COVID-19 or SARS-CoV-2)is a novel viral infection that started in December 2019 and has erupted rapidly in more than 150 *** rapid spread of COVID-19 has caused a global health emergency and resulted in governments imposing lock-downs to stop its *** is a signiﬁ-cant increase in the number of patients infected,resulting in a lack of test resources and kits in most *** overcome this panicked state of affairs,researchers are looking forward to some effective solutions to overcome this situa-tion:one of the most common and effective methods is to examine the X-radiation(X-rays)and computed tomography(CT)images for detection of ***-ever,this method burdens the radiologist to examine each ***,to reduce the burden on the radiologist,an effective,robust and reliable detection system has been developed,which may assist the radiologist and medical specia-list in effective detecting of *** proposed a deep learning approach that uses readily available chest radio-graphs(chest X-rays)to diagnose COVID-19 *** proposed approach applied transfer learning to the Deep Convolutional Neural Network(DCNN)model,Inception-v4,for the automatic detection of COVID-19 infection from chest X-rays *** dataset used in this study contains 1504 chest X-ray images,504 images of COVID-19 infection,and 1000 normal images obtained from publicly available medical *** results showed that the proposed approach detected COVID-19 infection with an overall accuracy of 99.63%.

关键词： COVID-19 transfer learning deep learning artiﬁcial intelligence chest X-rays machine learning

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