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血管紧张素II受体阻断剂的治疗新效应:刺激内皮祖细胞

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中国动脉硬化杂志 2005年第5期13卷 638-638页

作者： Bahl mann FH de Groot K mueller o Hertel B Haller H Fliser D 颜丽

A step-by-step protocol for formaldehyde-assisted isolation of regulatory elements from Arabidopsis thaliana

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Journal of Integrative Plant Biology 2014年第6期56卷 527-538页

作者： Mohammad Amin omidbakhshfard Flavia Vischi Winck Samuel Arvidsson Diego M.Ria?o-Pachón Bernd mueller-Roeber University of Potsdam Institute of Biochemistry and Biology Potsdam-Golm 14476 Germany Max-Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476 Germany Brazilian Bioethanol Science and Technology Laboratory (CTBE) Brazilian Center of Research in Energy and Materials (CNPEM) Campinas Brazil

The control of gene expression by transcriptional regulators and other types of functional y relevant DNA transactions such as chromatin remodeling and replication underlie a vast spectrum of biological processes in al organisms. DNA transactions require the control ed interaction of proteins with DNA sequence motifs which are often located in nucleosome-depleted regions （NDRs） of the chromatin. Formaldehyde-assisted isolation of regulatory elements （FAIRE） has been established as an easy-to-implement method for the isolation of NDRs from a number of eukaryotic organisms, and it has been successful y employed for the discovery of new regulatory segments in genomic DNA from, for example, yeast, Drosophila, and humans. Until today, however, FAIRE has only rarely been employed in plant research and currently no detailed FAIRE protocol for plants has been published. Here, we provide a step-by-step FAIRE protocol for NDR discovery in Arabidopsis thaliana. We demonstrate that NDRs isolated from plant chromatin are readily amenable to quantitative polymerase chain reaction and next-generation sequencing. only minor modification of the FAIRE protocol wil be needed to adapt it to other plants, thus facilitating the global inventory of regulatory regions across species.

关键词： Arabidopsis thaliana chromatin cis-regulatory elements epigenomics FAIRE-qPCR FAIRE-seq gene expression gene regulatory network transcription factor

Criminology[5E] / 5th ed.

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Solvation Structure and Dynamics of Mg(TFSI)_(2) Aqueous Electrolyte

2004年

作者： Freda Adler Gerhard o.W. mueller William S. Lauf

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Energy & Environmental Materials 2022年第1期5卷 295-304页

作者： Zhou Yu Taylor R.Juran Xinyi Liu Kee Sung Han Hui Wang Karl T.mueller Lin Ma Kang Xu Tao Li Larry A.Curtiss Lei Cheng Materials Science Division Argonne National LaboratoryLemont IL 60439USA Joint Center for Energy Storage Research(JCESR) Lemont IL 60439USA Department of Physics Binghamton UniversityState University of New York4400 Vestal Parkway EastBinghamton NY 13902USA Department of Chemistry and Biochemistry Northern Illinois UniversityDeKalb IL 60115USA Physical and Computational Sciences Directorate Pacific Northwest National LaboratoryRichland WA 99352USA Energy&Environment Directorate Pacific Northwest National LaboratoryRichland WA 99352USA Energy Storage Branch Energy and Biotechnology DivisionSensor and Electronics DirectorateU.S.Army Research LaboratoryAdelphi MD 20783USA Department of Chemistry and Biochemistry Northern Illinois University DeKalb IL 60115 USA X-ray Science Division Argonne National Laboratory Lemont IL 60439 USA

Using ab initio molecular dynamics(AIMD)simulations,classical molecular dynamics(CMD)simulations,small-angle X-ray scattering(SAXS),and pulsed-field gradient nuclear magnetic resonance(PFG-NMR),the solvation structure and ion dynamics of magnesium bis(trifluoromethanesulfonyl)imide(Mg(TFSI)_(2))aqueous electrolyte at 1,2,and 3 m concentrations are *** AIMD and CMD simulations,the first solvation shell of an Mg;ion is found to be composed of six water molecules in an octahedral configuration and the solvation shell is rather *** TFSI^(-)ions prefer to stay in the second solvation shell and ***,the comparable diffusion coefficients of positive and negative ions in Mg(TFSI)_(2)aqueous electrolytes have been observed,which is mainly due to the formation of the stable[Mg(H_(2)o_(6))_(2)]^(+)complex,and,as a result,the increased effective Mg ion ***,the calculated correlated transference numbers are lower than the uncorrelated ones even at the low concentration of 2 and 3 m,suggesting the enhanced correlations between ions in the multivalent *** work provides a molecular-level understanding of how the solvation structure and multivalency of the ion affect the dynamics and transport properties of the multivalent electrolyte,providing insight for rational designs of electrolytes for improved ion transport properties.

关键词： Mg(TFSI)_(2)aqueous electrolyte molecular dynamics simulation pulsed-field gradient nuclear magnetic resonance small-angle X-ray scattering ion dynamics

Could wastewater analysis be a useful tool for China?——A review

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Journal of Environmental Sciences 2015年第1期27卷 70-79页

作者： Jianfa Gao Jake o'Brien Foon Yin Lai Alexander L.N.van Nuijs Jun He Jochen F.mueller Jingsha Xu Phong K.Thai Faculty of Science and Engineering University of Nottingham Ningbo China National Research Centre for Environmental Toxicology The University of Queensland Toxicological Center University of Antwerp

Analysing wastewater samples is an innovative approach that overcomes many limitations of traditional surveys to identify and measure a range of chemicals that were consumed by or exposed to people living in a sewer catchment area. First conceptualised in 2001, much progress has been made to make wastewater analysis（WWA） a reliable and robust tool for measuring chemical consumption and/or exposure. At the moment, the most popular application of WWA, sometimes referred as sewage epidemiology, is to monitor the consumption of illicit drugs in communities around the globe, including China. The approach has been largely adopted by law enforcement agencies as a device to monitor the temporal and geographical patterns of drug consumption. In the future, the methodology can be extended to other chemicals including biomarkers of population health（e.g. environmental or oxidative stress biomarkers, lifestyle indicators or medications that are taken by different demographic groups） and pollutants that people are exposed to（e.g. polycyclic aromatic hydrocarbons,perfluorinated chemicals, and toxic pesticides）. The extension of WWA to a huge range of chemicals may give rise to a field called sewage chemical-information mining（SCIM） with unexplored potentials. China has many densely populated cities with thousands of sewage treatment plants which are favourable for applying WWA/SCIM in order to help relevant authorities gather information about illicit drug consumption and population health status. However, there are some prerequisites and uncertainties of the methodology that should be addressed for SCIM to reach its full potential in China.

关键词： Drug consumption Wastewater analysis Biomarkers Population health

Detection of Schistosoma mansoni DNA using polymerase chain reaction from serum and dried blood spot card samples of an adult population in North-western Tanzania

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Infectious Diseases of Poverty 2021年第1期10卷 66-73页

作者： Antje Fuss Humphrey DMazigo Andreas mueller Medical Mission Institute Hermann-Schell-Str.797074 WuerzburgGermany Department of Medical Parasitology and Entomology School of MedicineCatholic University of Health and Allied SciencesP.O.Box 1464MwanzaTanzania Department ofTropical Medicine Klinikum Wuerzburg Mitte gGmbHMedical Mission HospitalWuerzburgGermany.

Background:Real-time polymerase chain reaction(PCR)is a sensitive and specific method for diagnosing ***,this method should be performed in a laboratory,usually located distant from the sample collection ***,it is important to have fast sampling preservation methods,which allow simple transport prior to DNA extraction and *** aim of this study was to verify if blood samples applied to filter paper are suitable for analysis of Schistosoma mansoni DNA by real-time ***:A cross-sectional study was conducted among 100 study participants aged 17 to 70 years in a fishing village on the southern shore of Lake Victoria,*** samples and ethylenediaminetetraacetic acid(EDTA)-anticoagulated whole blood for preparation of dried blood spots(DBS)were collected to test for Schistosoma mansoni infection by real-time PCR.A combined diagnostic reference of positive results of serum-based real-time PCR and the Kato-Katz(KK)method was used for *** and negative predictive value(NPV)were *** Wilcoxon signed-rank test was chosen to compare the mean cycle threshold(Ct)values from serum and ***:According to the reference,92.5%*** positive samples were *** serum-based real-time PCR performed excellently with 95.4%sensitivity,whereas the DBS-based real-time PCR showed a low sensitivity(45.4%).The Ct-values were significantly higher in DBS(median:37.3)than in serum samples(median:27.5,Power parasite-specific DNA load on the filter *** increasing egg counts,an increase in sensitivity was observed for all *** PoC-CCA test and the serum-based real-time PCR showed a sensitivity of 100%for medium and severe *** DBS real-time PCR showed a sensitivity of only 85.7%even for severe ***:DBS-based real-time PCR did not provide good results in our study and therefore should not be recommended or must be tested concerning tempe

关键词： Schistosoma mansoni Real-time PCR Circulating DNA Dried blood spots Tanzania

Evaluation of serum-based real-time PCR to detect Schistosoma mansoni infection before and after treatment

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Infectious Diseases of Poverty 2020年第3期9卷 114-120页

作者： Antje Fuss Humphrey Deogratias Mazigo Andreas mueller Medical Mission Institute Hermann-Schell-Str.797074 WuerzburgGermany School of Medicine Department of Medical Parasitology&EntomologyCatholic University of Health and Allied SciencesP.O.Box 1464MwanzaTanzania Klinikum Wuerzburg Mitte gGmbH Medical Mission HospitalDept.of Tropical MedicineWuerzburgGermany

Background:To detect acute schistosomiasis,low-intensity infections,or to verify the success of treatment with praziquantel,highly sensitive test methods are *** aim of this study was therefore to demonstrate the performance of Schistosoma mansoni specific DNA detection in serum and urine using real-time polymerase chain reaction(PCR)in an endemic area before and after ***:The study pursued a 1-week and 20-weeks longitudinal design with a treatment intervention among 36 study participants aged 18 to 70 years in the community of Kayenze,a fishing village in Ilemela district on the southern shore of Lake Victoria in north-western Tanzania between February and June ***,urine and stool samples were collected from each participant to diagnose Schistosoma mansoni infection before and two times after treatment with praziquantel using serum-and urine based real-time PCR,point-of-care circulating cathodic antigen(PoC-CCA)rapid diagnostic test and the microscopic Kato-Katz(KK)*** coefficient(κ)was used to estimate the agreement between these diagnostic tests compared to a combined“gold standard”of positive results by serum-based real-time PCR and/or positive egg counts determined by ***’s Tau rank correlation was used to examine the relationship between cycle threshold(Ct)-values and egg counts and the Wilcoxon signed-rank test was used to compare the median Ct-values of the different examination time ***:By using the combined“gold standard”of the parasitological Kato-Katz test and/or serum-based real-time PCR,a *** prevalence of 77.1%could be determined at *** terms of sensitivity,serum-based realtime PCR(96.3%)and PoC-CCA assay(77.8%)showed the highest *** detection of DNA from urine samples showed the lowest sensitivity(33.3%).Treatment with praziquantel resulted in a significantly reduced prevalence of *** infection could be detected by Kato-Katz,with the PoC-CCA test only 33.3%.The an

关键词： Schistosoma mansoni Real-time PCR Circulating DNA Tanzania

oRS1, an H2o2-Responsive NAC Transcription Factor, Controls Senescence in Arabidopsis thaliana

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Molecular Plant 2011年第2期4卷 346-360页

作者： Salma Balazadeh Miroslaw Kwasniewski Camila Caldana Mohammad Mehrnia Maria Ines Zanor Gang-Ping Xue Bernd mueller-Roeber University of Potsdam Institute of Biochemistry and Biology KarI-Liebknecht-Straβe 24-25 Haus 20 14476 Potsdam-Golm Germany Max-Planck Institute of Molecular Plant Physiology Am Muhlenberg 1 14476 Potsdam-Golm Germany University of Silesia Department of Genetics Jagiellonska 28 40-032 Katowice Poland CSIRo Plant Industry 306 Carmody Road St Lucia QLD 4067 Australia Present address: Instituto de Biologia Molecular y Celular de Rosario (IBR-CoNICET). Facultad de Ciencias Bioquimicas y Farmaceuticas (UNR) Suipacha 530 Rosario S2002LRK Argentina

We report here that oRS1, a previously uncharacterized member of the NAC transcription factor family, controls leaf senescence in Arabidopsis thaliana. overexpression of oRS1 accelerates senescence in transgenic plants, whereas its inhibition delays it. Genes acting downstream of oRS1 were identified by global expression analysis using transgenic plants producing dexamethasone-inducible oRSl-GR fusion protein. of the 42 up-regulated genes, 30 （-70%） were pre- viously shown to be up-regulated during age-dependent senescence, We also observed that 32 （-76%） of the oRSl-dependent genes were induced by long-term （4 d）, but not short-term （6 h） salinity stress （150 mM NaCI）. Furthermore, expression of 16 and 24 genes, respectively, was induced after 1 and 5 h of treatment with hydrogen peroxide （H2o2）, a reactive oxygen species known to accumulate during salinity stress. oRS1 itself was found to be rapidly and strongly induced by H2o2 treatment in both leaves and roots. Using in vitro binding site selection, we determined the preferred binding motif of oRS1 and found it to be present in half of the oRSl-dependent genes. oRS1 is a paralog of oRE1/ ANACo92/AtNAC2, a previously reported regulator of leaf senescence. Phylogenetic footprinting revealed evolutionary conservation of the oRS1 and oRE1 promoter sequences in different Brassicaceae species, indicating strong positive selection acting on both genes. We conclude that oRS1, similarly to oRE1, triggers expression of senescence-associated genes through a regulatory network that may involve cross-talk with saltand H2o2-dependent signaling pathways.

关键词： NAC transcription factor leaf senescence gene expression gene regulatory network hydrogen peroxide

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Making The Grade