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Chemical Research in Chinese Universities 2007年第1期23卷 31-34页

作者： LUE Chun-sheng ZHANG Yue-tao mu ying Key Laboratory for Supramolecular Structure and Materials of Ministry of Education School of Chemistry Jilin University 130012 P. R. China Department of Chemical Engineering Daqing Petroleum Institute Daqing 161310 P. R. China

The copolymerization of ethylene with dieyclopentadiene （DCP） in the presence of a constrained geometry tetramethylcyclopentadi-enyl-phenoxytitanium catalyst [ 2,4-＇ Bu2-6-（ 2,3,4,5-Me4 -Cp ） -PhO ] TICl2, combined with AI（ iBu）3/Ph3C^＋ B（ CsF5 ）4^- cocatalyst system was studied. The copolymers that were formed were characterized by ＇ H and ,3 C NMR, differential scanning calorimetry （ DSC）, SEM, and X-ray diffraction （XRD） analyses. The re- suits of the analysis indicate that the copolymers of ethylene with dicyclopentadiene are amorphous and display two or more melting temperatures in their DSC diagrams. Moreover, the morphologies of the copolymers are quite different from that of polyethylenes.

关键词： Ethylene polymerization Dicyclopentadiene Copolymers Metalloeene Polyethylene

Rapid Absolute Determination Platform of Nucleic Acid for Point-of-care Testing

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Chemical Research in Chinese Universities 2015年第4期31卷 519-525页

作者： SONG Bofan JIN Wei SONG Qi JIN Qinhan mu ying Research Center for Analytical Instrumentation State Key Laboratory of lndustrial Control Technology Zhejiang University Hangzhou 310058 P. R. China

Nowadays nucleic acid tests are promising to be considered as point-of-care testing（POCT）. However, no such devices are currently available that can perform all the functions, including absolute nucleic acid determination, worldwide on-site detection, rapid analysis and real-time results reporting via ubiquitous mobile networks simultaneously with full package and automated means of measurement. In this study, we presented a compact low-cost portable POC automated testing platform with all attributes mentioned above. A disposable self-priming compartmentalization（SPC） microfluidic chip is used to conduct isothermal amplification. The platform also includes a micro-computer controlled heating unit, an inexpensive optical imaging setup, and a mobile device with customized software. It may become a useful tool for the rapid on-site detection of infectious diseases as well as other pathogens.

关键词： Point-of-care testing Microfluidic chip Loop-mediated isothermal amplification Mobile medicine

Easily Accessible and Highly Selective ＂Turn-on＂ Fluorescent Sensor for Imaging Cadmium in Living Cells

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Chemical Research in Chinese Universities 2013年第4期29卷 632-637页

作者： WANG Wei ZHANG ying-mu LI Yao-xian ZHAO Qing Department of Neurology China-Japan Union Hospital of Jilin University Changehun 130031 P. R. China College of Chemistry Jilin University Changehun 130012 P. R. China

A new schiff base of phenothiazine derivative was designed for ratiometric sensing of Cd2＋ selectively. Upon the addition of Cd2＋ to the solution of phenothiazine derivative, the fluorescence intensity of it was enhanced in a linear fashion and the maximum fluorescence peak exhibited a blue shift from 575 nm to 525 nm. This ratiometric fluorescent sensor displays a very high sensitivity（detection limits were 0.34 and 1.0μmol/L of Cd2＋ using the visual fluorescence changes and UV-Vis changes, respectively）, a rapid response time（〈10 s） and high selectivity for Cd2＋ over other transition metal ions. Moreover, the living cells image experiments also demonstrate the value of the sen- sor in fluorescent visualization of Cd2＋ in the environmental and biological systems.

关键词： Fluorescent sensor Blue-shift Cadmium Phenothiazine Cell-imaging

PDE10A inhibitor in addiction

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中国药理学与毒理学杂志 2017年第5期31卷 455-456页

作者： mu ying ZHEN Xue-chu Jiangsu Key Laboratory of translational research for Neuro-psychiatric diseases College of Pharmaceutical SciencesSoochow University

Chronic exposure to drugs of abuse will give rise to persistent structural and functional changes in the central nervous system. These phenomena are usually referred as ′drug-induced neuroplasticity′ and depend on changes in gene expression. The cAMP response element binding protein(CREB),as a downstream molecule in mediating the actions of cAMP is an important transcriptional factor in establishing and maintaining addiction to drugs of abuse. Application of a PDE4 inhibitor attenuates the rewarding properties of cocaine and morphine. Given the fact that PDE10A is specifically located in striatum,an important structure involved in the reward circuit,we thus investigated the PDE10A inhibitormodulated the behavioral reinforcement exerted by morphine. The results show that MP-10 2.5 mg·kg^(-1),administered subcutaneously,significantly inhibited the acquisition of morphine-induced CPP. Moreover,MP-10 did not alter the expression of morphine-induced CPP,but did accelerate the extinction of morphine-induced CPP. Additionally,chronic treatment with MP-10 2.5 mg·kg^(-1)decreased expression of phosphorylated CREB(pC REB) in dorsomedial striatum,in shell of NAc,and in anterior cingulate cortex(ACC) as well as decreased expression of ΔFos B in the shell of NAc and ACC. These data indicate that PDE10A inhibition may have a potential therapeutic effect on addiction. Since the MP-10 has relative short metabolic Stability,we also developed a few novel potent new PDE10A selective inhibitor with improved stability and brain exposure. The new compound exhibited promising potential in schizophrenia and addiction treatment.

关键词： PDE10A inhibitor in addiction

Increased sensitivity to drought stress in atlon4 Arabidopsis mutant

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Chinese Science Bulletin 2010年第32期55卷 3668-3672页

作者： LI XiaoYi mu ying SUN XuWu ZHANG LiXin School of Life Sciences Lanzhou University Lanzhou 730000 China Photosynthesis Research Center Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China

The ATP-dependent serine protease Lon belongs to the AAA+ superfamily,which is widely distributed in bacteria,archaea,and eukaryotic cells. Lon participates in the regulation of numerous physiological processes and gene expression via degrading various unstable and non-native regulators. In this study,we showed that the Arabidopsis atlon4 mutant is more sensitive to drought stress than wild-type plants. Compared with wild-type plants,atlon4 mutant plants showed increased water loss,decreased water use efficiency,and impaired stomatal closure in drought stress conditions.

关键词：干旱胁迫突变体敏感性拟南芥丝氨酸蛋白酶监管机构水分流失真核细胞

Immunosensor Based on Surface Plasmon Resonance for Antigen Recognition

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Chemical Research in Chinese Universities 2008年第1期24卷 24-28页

作者： JIN Wei mu ying ZOU Xiang-yu JIN Qin-han Research Center for Analytical Instrumentation College of Information Science and Engineering Zhejiang University Hangzhou 310058 P. R. China College of Instrumentation and Electrical Engineering Jilin University Changchun 130021 P. R. China College of Chemistry Jilin University Changchun 130021 P. R. China

A novel immunosensor based on surface plasmon resonance（SPR） has been developed for the recognition of antigen. The sensor was designed on the basis of the fixed angle of incidence and measuring the reflected intensities in a wavelength range of 430--750 nm in real-time. An ultra-bright white light-emitting diode（LED） was used as the light source. Molecular self-assembling in solution was used to form the sensing membrane on gold substrate. It has been seen that the sensitivity of the SPR sensor with 3-mercaptopropionic acid（MPA）/protein A（SPA） sensing membrane is considerably higher than that with MPA or SPA modified sensing membrane. The kinetic processes on the sensing membrane were studied. The human B factor（Bf）, an activator of complement 3（C3）, was recognized among the other antigens. This sensor can also be used for other antigen/antibody or adaptor/receptor recognition. Under optimized experimental conditions, the sensor has good selectivity, repeatability, and reversibility.

关键词： Surface plasmon resonance Immunosensor Molecular recognition

WLAN indoor location method based on artificial neural networkt

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High Technology Letters 2010年第3期16卷 227-234页

作者： Zhou mu Sun ying Xu Yubin Deng Zhian Meng Weixiao Communication and Research Center School of Electronics and Information Technology Harbin Institute of Technology Harbin 150001 P.R. China

WLAN mdoor location method based on artificial neural network （ANN） is analyzed. A three layer feed-forward ANN model offers the benefits of reducing time cost of the layout of an indoor location system, saving storage cost of the radio map establishment and enhancing real-time capacity in the on-line phase. According to the analysis of SNR distributions of recorded beacon signal samples and discussion about the multi-mode phenomenon, the one map method is proposed for the purpose of simplifying ANN input values and increasing location performances. Based on the simulations and comparison analysis with other two typical indoor location methods, K-nearest neighbor （KNN） and probability, the feasibility and effectiveness of ANN-based indoor location method are verified with average location error of 2.37m and location accuracy of 78.6% in 3m.

关键词： indoor location WLAN artificial neural network (ANN) multi-mode fingerprint

Syntheses, Structures, and Blue Luminescent Properties of 2,6-Bis-(benzimidazolyl) Pyridine and Its Mercury Complex

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Chemical Research in Chinese Universities 2007年第2期23卷 159-162页

作者： LIU Xiao-ming mu Xiao-yue XIA Hong SU Qing YE Ling CHEN Chao GAO Wei mu ying Key Laboratory for Supramolecular Structure and Materials of Ministry of Education College of Chemistry Jilin University Changchun 130012 P. R. China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Changchun 130012 P. R. China

A novel blue luminescent mercury （ Ⅱ ） complex of 2,6-bis （benzimidazolyl） pyridine （L） was synthesized. In solid state, this complex has the formula [Hg（L）Cl2] · 2DMF（1）. The molecular structures of both the ligand and the complex were determined by using tingle-crystal X-ray diffraction. Crystallographic studies of the mercury （ Ⅱ） complex reveal that the complex possesses a five-coordinated geometry that can be best described as a distorted trigonal-bipyramidal. The luminescent properties of ligand L and complex 1 both in solution and in solid state were also studied.

关键词： 2,6-Bis (benzimidazolyl) pyridine Mercury Crystal structure Blue luminescence

Rapid Selection of Phage Se-scFv with GPX Activity via Combination of Phage Display Antibody Library with Chemical Modification

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Chemical Research in Chinese Universities 2007年第1期23卷 58-63页

作者： LIN Feng LI ying YANG Wen-kui LIANG Bing mu ying SUN Ye LI Wei LUO Gui-min Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education College of Life Science Jilin University Changchun 130021 P. R. China

Glutathione peroxidase（GPX） plays an important role in scavenging reactive oxygen species. A series of catalytic antibodies with GPX activity have been generated by the authors of＇ this study. To obtain humanized catalytic antibodies, the phage-displayed human antibody library was used to select novel antibodies by repetitive screening, Phage antibodies, scFv-B8 and scFv-H6 with the GSH-binding site, were obtained from the library by enzyme-linked immu- nosorbent assay（ELISA） analysis with 4 rounds of scelection against their respective haptens, S-2,4-dinitriphenyl t-butyl ester（GStI-s-DNP-Bu） and S-2,4-dinit,-iphenyl t-hexyl ester（GSH-s-I）NP-He）. Nevertheless, several studies need to be condueted to determine whether scFv-B8 and seFv-tI6 possess GPX activity. 1＇o enhance the speed of the selection, selenocysteine（Sec, the catalytic group of GPX） was incorporated directly into the phages, scFv-B8 and seFv-H6, by chemical mutation to form the phages Se-scFv-B8 and Se-scFv-H6. The GPX activities were found to be 3012 units/μmol and 2102 units/μmol, respectively. To improve the GPX activity of the phage Se-scFv-B8, DNA shuffling was used to construct a secondary library and another positive phage antibody scFv-B9 was screened out by another panning against GSH-s-DNP-Bu. When Sec was incorporated via chemical mutation into the phage antibody scFv-B9, its GPX activity reached 3560 units/μmol, which is 1.17-fold higher than the phage antibody Se-scFv-B8 and almost approached the order of magnitude of native GPX. The rapid selection is the prerequisite for generating humanized Se-seFv with GPX activity.

关键词： Single chain Fv Chemical modification DNA shuffling Glutathione peroxidase Phage display antibody library Selection Selenium Antibody humanization

The impact of migration on the food consumption and nutrition of left-behind family members: Evidence from a minority mountainous region of southwestern China

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Journal of Integrative Agriculture 2019年第8期18卷 1780-1792页

作者： MIN Shi HOU Ling-ling Hermann Waibel HUANG Ji-kun mu Yue-ying China Center for Agricultural Policy School of Advanced Agricultural Sciences Peking University Beijing 100871 P.R.China Institute of Development and Agricultural Economics Leibniz University Hannover Hannover 30167 Germany College of Economics and Management China Agricultural University Beijing 100083 P.R.China

While migration is widely recognized as a valid option for improving farmers’ income, the welfare effects of migration on left-behind family members are ambiguous. This study examines the impacts of migration on left-behind family members’ food consumption and nutrient intake, particularly in remote rural areas in China. Based on household survey data collected from 611 smallholder rubber farmers in Xishuangbanna Dai Autonomous Prefecture of southwestern China, the empirical results suggest that the migration of family members contributes to improving household net income, whereas it negatively affects left-behind family members’ consumption of grain and pork. Migration also leads to a decrease in left-behind family members’ nutrient intake. Surprisingly, the economic returns of farmers’ migration not only do not foster the transformation of household food consumption from a staple food-dominated dietary structure to one including more meat and dairy products but also reduce left-behind family members’ nutrient intake. This study adds to the literature on the impact of farmers’ migration. The findings have important implications for better understanding the impacts of migration on farmers’ livelihood and human capital development in rural China.

关键词： migration food consumption nutrition left-behind