Immunosensor Based on Surface Plasmon Resonance for Antigen Recognition

作     者：JIN Wei MU Ying ZOU Xiang-yu JIN Qin-han 

作者机构：Research Center for Analytical Instrumentation College of Information Science and Engineering Zhejiang University Hangzhou 310058 P. R. China College of Instrumentation and Electrical Engineering Jilin University Changchun 130021 P. R. China College of Chemistry Jilin University Changchun 130021 P. R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2008年第24卷第1期

页      面：24-28页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 071009[理学-细胞生物学] 070305[理学-高分子化学与物理] 09[农学] 090102[农学-作物遗传育种] 0710[理学-生物学] 0817[工学-化学工程与技术] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0901[农学-作物学] 0703[理学-化学] 

基　　金：National Key Technologies Research & Development Program of China(No.2006BAK03A09) National Basic Research Program of China(No.2007CB714503) Science and Technology Development Program of Jilin Province, China (No.20060706) 

主　　题：Surface plasmon resonance Immunosensor Molecular recognition 

摘      要：A novel immunosensor based on surface plasmon resonance（SPR） has been developed for the recognition of antigen. The sensor was designed on the basis of the fixed angle of incidence and measuring the reflected intensities in a wavelength range of 430--750 nm in real-time. An ultra-bright white light-emitting diode（LED） was used as the light source. Molecular self-assembling in solution was used to form the sensing membrane on gold substrate. It has been seen that the sensitivity of the SPR sensor with 3-mercaptopropionic acid（MPA）/protein A（SPA） sensing membrane is considerably higher than that with MPA or SPA modified sensing membrane. The kinetic processes on the sensing membrane were studied. The human B factor（Bf）, an activator of complement 3（C3）, was recognized among the other antigens. This sensor can also be used for other antigen/antibody or adaptor/receptor recognition. Under optimized experimental conditions, the sensor has good selectivity, repeatability, and reversibility.