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Gold nanoparticle-assisted delivery of brain-derived neurotrophic factor to cerebral organoids

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Nano Research 2022年第4期15卷 3099-3105页

作者： Sung Bum Park Hye-Jin Cho Sang Rin moon Kyoung Jin Choi Won Hoon jung ki Young kim Byumseok Koh Therapeutics and Biotechnology Division Korea Research Institute of Chemical Technology141 Gajeong-roYuseong-guDaejeon 34114Republic of Korea Chemical Platform Technology Division Korea Research Institute of Chemical Technology141 Gajeong-roYuseong-guDaejeon 34114Republic of Korea

Gold nanoparticle(AuNP)-assisted delivery of proteins/drugs to cells or in vivo systems has long been ***,their interaction with in vivo mimicking organoids is still largely ***,we conjugated brain-derived neurotropic factor(BDNF)to the AuNP surface and monitored how AuNP-BDNF interacts with cerebral *** suggest that AuNP-BDNF can successfully enter cerebral organoids and can be found up to 15.21μm inside cerebral ***-BDNF does not induce significant cytotoxicity to cerebral organoids at concentrations up to 2 nM and increases the expression of 75 genes compared to control cerebral *** data suggest the successful delivery of BDNF through AuNP-BDNF conjugation in cerebral organoids,and the conjugation can affect its differentiation.

关键词： gold nanoparticles cerebral organoids delivery penetration differentiation viability

The optimal timing of post-prostate biopsy magnetic resonance imaging to guide nerve-sparing surgery

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Asian Journal of Andrology 2014年第2期16卷 280-284,I0010页

作者： Young Hwii Ko Phil Hyun Song ki Hak moon Hee Chang jung Jun Cheon Deuk Jae Sung Department of Urology Yeuflgnam University School of Medicine Daegu Korea Department of Urology Korea University School of Medicine Seoul Korea Department of Radiology Korea University School of Medicine Seoul Korea

The goal of our study was to evaluate the impact of the interval between prostate biopsy and magnetic resonance imaging （MRI） on the accuracy of simple tumor localization, which is essential information that enables nerve-sparing surgery. We also sought to determine the optimal timing of a post-biopsy MRI, A total of 184 patients who had undergone MRI before radical prostatectomy at an institution without a predetermined schedule for MRI after a prostate biopsy were enrolled. The mean interval from the biopsy to the MRI was 30.8 ± 18.6days. The accuracy of the MRI for simplified tumor location （right, left, bilateral and none） was 44.6%. In the group with discordant pathologic and MRI findings, the most common reason recorded was ＇MRI predicted a unilateral lesion, but pathology revealed bilateral lesions＇（58.3%）, followed by ＇MRI predicted no lesion, but pathology revealed the presence of a lesion＇（32.0%）. Multivariable analysis showed that the discordant group had a shorter interval （25.0 ± 14.3 vs 38.1 ± 20.6days, P 〈 0.01） preceding the MRI and a higher rate of hemorrhage as observed by MRI （80.4% vs 54.8%, P 〈 0.01） in comparison with the accordant group. In receiver operating characteristics analysis, the area under the curve of the MRI interval in accurate prediction of the tumor location was 0.707 （P 〈 0.001）. At the MRI interval＇s cutoff of 28.5days, the sensitivity was 73.2% and the specificity was 63.7%. When the MRI was performed within 28days, the accumulated accuracy was only 26.1% （23/88）; however, when it was performed after 28days, the reversely accumulated accuracy was 61.5% （59/96）. These data support a waiting period of at least 4weeks after a biopsy before performing an MRI for the purposes of surgical refinement.

关键词： magnetic resonance imaging nerve preservation prostate biopsy radical prostatectomy

Rice tissue-specific promoters and conditiondependent promoters for effective translational application

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Journal of Integrative Plant Biology 2015年第11期57卷 913-924页

作者： Hee-Jeong Jeong ki-Hong jung Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute Kyung Hee University Graduate School of Biotechnology Kyung Hee University

Rice （Oryza sativa） is one of the most important staple food crops for more than half of the world＇s population. The demand is increasing for food security because of population growth and environmental challenges triggered by climate changes. This scenario has led to more interest in developing crops with greater productivity and sustainability. The process of genetic transformation, a major tool for crop improvement, utilizes promoters as one of its key elements. Those promoters are generally divided into three types： constitutive, spatiotemporal, and condition-dependent. Tran- scriptional control of a constitutive promoter often leads to reduced plant growth, due to a negative effect of accumu- lated molecules during cellular functions or energy consump- tion. To maximize the effect of a transgene on transgenic plants, it is better to use condition-dependent or tissue- specific promoters. However, until now, those types have not been as widely applied in crop biotechnology. In this review, we introduce and discuss four groups of tissue-specific promoters （5o promoters in total） and six groups of condition-dependent promoters （27 promoters）. These pro- moters can be utilized to fine-tune desirable agronomic traits and develop crops with tolerance to various stresses, enhanced nutritional value, and advanced productivity.

关键词： Condition-dependent promoters crop biotechnologyapplication genetic transformation rice tissue-specific promoters

Process-Based Crop Modeling for High Applicability with Attention Mechanism and Multitask Decoders

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Plant Phenomics 2023年第2期5卷 378-390页

作者： Taewon moon Dongpil kim Sungmin Kwon jung Eek Son Department of Agriculture Forestry and BioresourcesSeoul National UniversitySeoul 08826Republic of Korea Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoul 08826Republic of Korea Protected Horticulture Research Institute National Institute of Horticultural&Herbal ScienceRural Development AdministrationHaman 52054Republic of Korea

Crop models have been developed for wide research purposes and scales,but they have low compatibility due to the diversity of current modeling *** model adaptability can lead to model *** deep neural networks have no conventional modeling parameters,diverse input and output combinations are possible depending on model *** these advantages,no process-based crop model has been tested in full deep neural network *** objective of this study was to develop a process-based deep learning model for hydroponic sweet *** mechanism and multitask learning were selected to process distinct growth factors from the environment *** algorithms were modified to be suitable for the regression task of growth *** were conducted twice a year for 2 years in *** developed crop model,DeepCrop,recorded the highest modeling efficiency(=0.76)and the lowest normalized mean squared error(=0.18)compared to accessible crop models in the evaluation with unseen *** t-distributed stochastic neighbor embedding distribution and the attention weights supported that DeepCrop could be analyzed in terms of cognitive *** the high adaptability of DeepCrop,the developed model can replace the existing crop models as a versatile tool that would reveal entangled agricultural systems with analysis of complicated information.

关键词： Deep replace versatile

Consistency of intensity ratio between spectral lines with similar self-absorption characteristics during ungated laser induced breakdown spectroscopy measurements

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Plasma Science and Technology 2019年第3期21卷 77-83页

作者： jung-Hwan IN Youngmin moon Jang-Hee CHOI Sungho JEONG Optical Lens Research Center Korea Photonics Technology Institute School of Mechanical Engineering Gwangju Institute of Science and Technology

This work reports that the intensity ratio of spectral lines having similar self-absorption characteristics during laser induced breakdown spectroscopy(LIBS) analysis can become nearly constant over a wide range of irradiation conditions if the intensities are integrated over a sufficiently long time. It is shown that the plasma temperature and intensity ratio of these spectral lines have temporal similarity. The spectral lines with similar self-absorption properties may be selected to improve the accuracy and consistency of LIBS analysis results under an environment with fluctuating measurement conditions.

关键词： laser induced breakdown spectroscopy intensity ratio self-absorption characteristics CIGS solar cell

Machine Learning-Based Two-Stage Data Selection Scheme for Long-Term Influenza Forecasting

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Computers, Materials & Continua 2021年第9期68卷 2945-2959页

作者： Jaeuk moon Seungwon jung Sungwoo Park Eenjun Hwang School of Electrical Engineering Korea UniversitySeoul02841Korea

One popular strategy to reduce the enormous number of illnesses and deaths from a seasonal influenza pandemic is to obtain the influenza vaccine on ***,vaccine production preparation must be done at least six months in advance,and accurate long-term influenza forecasting is essential for *** diverse machine learning models have been proposed for influenza forecasting,they focus on short-term forecasting,and their performance is too dependent on input *** a country’s longterm influenza forecasting,typical surveillance data are known to be more effective than diverse external data on the *** propose a two-stage data selection scheme for worldwide surveillance data to construct a longterm forecasting model for influenza in the target *** the first stage,using a simple forecasting model based on the country’s surveillance data,we measured the change in performance by adding surveillance data from other countries,shifted by up to 52 *** the second stage,for each set of surveillance data sorted by accuracy,we incrementally added data as input if the data have a positive effect on the performance of the forecasting model in the first *** the selected surveillance data,we trained a new longterm forecasting model for influenza and perform influenza forecasting for the target *** conducted extensive experiments using six machine learning models for the three target countries to verify the effectiveness of the proposed *** report some of the results.

关键词： Influenza data selection machine learning forecasting

Effect of the herbal medicine Danggui Sini plus Wuzhuyu Shengjiang Tang on erythrocyte deformability in normal subjects: a cross-over trial

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Journal of Traditional Chinese Medicine 2018年第5期38卷 769-772页

作者： JiEun Lee Seung-Yeon Cho Seong-Uk Park Woo-Sang jung Sang-Kwan moon jung-Mi Park Chang-Nam Ko ki-Ho Cho Seungwon Kwon Department of Cardiology and Neurology College of Korean Medicine Kyung Hee University

OBJECTIVE: To show whether Danggui Sini plus Wuzhuyu Shengjiang Tang(DSWST) has any transient effect on erythrocyte deformability in normal ***: A total of 25 subjects [mean age(27.8± 1.8) years] was enrolled in this study. The study was designed as a cross-over trial in which the subjects took part for 2 d. On the first day, blood samples were collected at baseline and 1-2 h after administration of water, whereas, on the second day, instead of water, the subjects were administered DSWST after the baseline blood sampling. The blood samples collected at baseline and after the administration water or DSWST, were examined for erythrocyte ***: The elongation index increased significantly after 2 h(P = 0.009) compared to the baseline after DSWST intake. However, after water intake, there was no significant differenceobserved. When comparing the percent change of erythrocyte deformability between DSWST and water, we found that after 2 h of administration,DSWST improved erythrocyte deformability significantly compared to water(P < 0.001).CONCLUSION: DSWST has a transient effect on erythrocyte deformability in normal subjects.

关键词： Erythrocyte deformability Peptide elongation factors Raynaud disease Coldness of hands and feet Cross-over studies Danggui Sini plus Wuzhuyu Shengjiang Tang

Uhwang Chungsim Won Decreases Blood Oxygen Level-Dependent fMRI Signal Response to A Motor Stimulation Task

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Chinese Journal of Integrative Medicine 2015年第7期21卷 493-499页

作者： Woo-Sang jung Jong-Man Ryu Young-Jee kim Seong-Uk Park Geon-Ho Jahng jung-Mi Park Sang-Kwan moon Chang-Nam Ko ki-Ho Cho Department of Cardiovascular&Neurologic Diseases(Stroke Center) College of Oriental MedicineKyung Hee University Department of Radiology College of MedicineKyung Hee University

Objectives： TO evaluate the effect of Uhwang Chungsim Won [UC, Niuhuang Qingxin Pill （牛黄清心丸] on the blood oxygen level-dependent （BOLD） functional magnetic resonance imaging （fMRI） signal response to a motor stimulation task using a hand grasping paradigm. Methods： Ten healthy right-handed male volunteers were recruited. Their BOLD fMRI signal characteristics during hand grasping were determined before and 1 h after consuming UC. Results： With the administration of UC, BOLD responses to the right hand motor task showed a decrease in both hemispheres including the right cerebellar culmen, right and left medial frontal gyrus, right parahippocampal gyrus, right and left sublobar insula, right and left posterior cingulate, left precuneus, and right precentral gyrus. Decreased BOLD responses to the left hand motor task were shown in the right lingual gyrus, right cerebellar culmen, left superior temporal gyrus, right and left middle frontal gyrus, and right precentral gyrus after UC administration. Increased BOLD responses to the left hand motor task were shown in the left and right posterior cingulate, while there was no increased response to the right hand motor task after UC administration. Conclusions： UC administration significantly decreased fMRI BOLD signal responses to hand grasping in most of the brain areas including the primary motor cortex, but it is not clear whether these decreased responses were caused by a decrease in neuronal activities or by an increase in cerebral blood flow due to the cerebral vasodilatory effects of UC. Further studies using positron emission tomography or single photon emission computed tomography are needed to fully elucidate these findings.

关键词： Uhwang Chungsim Won Niuhuang Qingxin Pfll pharmacological fMRI cerebral blood flow

Neuroprotective Effect of Chunghyuldan(Qing Xue Dan)on Hypoxia-Reoxygenation Induced Damage of Neuroblastoma 2a Cell Lines

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Chinese Journal of Integrative Medicine 2013年第12期19卷 940-944页

作者： Chang-Nam Ko In-Seo Park Seong-Uk Park Woo-Sang jung Sang-Kwan moon jung-Mi Park Chulhun Kang ki-Ho Cho Department of Cardiology and Neurology Diseases College of Korean MedicineKyung Hee University Department of Medical Science Graduate School of East-West Medical ScienceKyung Hee University

Objectives： Chunghyuldan （CHD）, a combinatorial drug that has anti-hyperlipidemic and anti inflammatory activities, has been shown to reduce infarct volume in a focal ischemia-reperfusion rat model. To explore the molecular basis of CHD＇s neuroprotective effect, we examined whether CHD shows a cell-protective activity and has a regulatory effect on Bax and/or B-cell leukemia/lymphoma 2 （Bcl-2） expression in mouse neuroblastoma 2a （N2a） cells subjected to hypoxia-reoxygenation （H/R）. Methods： In order to evaluate the effects of CHD on the cytotoxicity induced from hypoxia or H/R condition, lactate dehydrogenase （LDH） assay was performed. To explore whether the suppression of neural damage when pre-treated with CHD is associated with its anti-apoptotic effect, the CHD effect on the expression of Bcl-2 and Bax was analyzed by Western blotting analysis. Results： Cytotoxicity of N2a cell line was slightly increased in 42 h hypoxia condition and dramatically increased under the H/R condition. CHD treatment markedly decreased the cytotoxicity in both conditions （P〈0.01, P〈0.05）. H/R markedly increased the expression of the pro-apoptotic protein, Bax, but slightly increased the expression of the anti-apoptotic protein, Bcl-2, compared with the normoxia or hypoxia group. CHD significantly decreased Bax expression （P〈0.01） and slightly decreased Bcl-2 expression （P〉0.05）, resulted in a reduction of Bax/Bcl-2 ratio in N2a cells subjected to H/R. Conclusion： CHD has neuroprotective effect in N2a cells subjected to H/R, which might be derived at least in part from its ability to decrease the expression of the pro-apoptotic protein, Bax.

关键词： apoptosis stroke Chunghyuldan herbal