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Char.cter.zation of the voltage-gated sodium channel of the Asian citr.s psyllid, Diaphor.na citr.

Insect Science 2017年第1期24卷 47-59页

作者： Bin Liu monique r. coy Jin-Jun Wang Lukasz L. Stelinski Entomology and Nematology Depar.ment Citrus Research and Education Center University of Florida Florida USA Key Labor.tor. of Entomology and Pest Contr.l Engineer.ng College of Plant Protection Southwest University Chongqing China

The Asian citr.s psyllid, Diaphor.na citr. Kuwayama （Hemipter.： Liviidae）, is an impor.ant insect pest of citr.s. It is the vector.of ＇Candidatus＇ Liber.bacter.asiaticus, a phloem-limited bacter.um that infects citr.s, r.sulting in the disease Huanglongbing （HLB）. Disease management r.lies heavily on suppr.ssion of D. cir.i populations with insecticides, including pyr.thr.ids. In r.cent annual sur.eys to monitor.insecticide r.sistance, r.duced susceptibility to fenpr.pathr.n was identified in sever.l field populations olD. citr.. The pr.mar. tar.et ofpyr.thr.ids is the voltage-gated sodium channel （VGSC）. The VGSC is pr.ne to tar.et-site insensitivity because of mutations that either.r.duce pyr.thr.id binding and/or.alter.gating kinetics. These mutations, known as knockdown r.sistance or.kdr. have been r.por.ed in a wide diver.ity of ar.hr.pod species. Alter.ative splicing, in combination with kdr.mutations, has been also associated with r.duced pyr.thr.id efficacy. Her. we r.por. the molecular.char.cter.zation of the VGSC in D. citr. along with a sur.ey of alter.ative splicing acr.ss developmental stages of this species. Pr.vious studies demonstr.ted that D. citr. has an exquisite enzymatic ar.enal to detoxify insecticides r.sulting in r.duced efficacy. The r.sults fr.m the cur.ent investigation demonstr.te that tar.et-site insensitivity is also a potential basis for.insecticide r.sistance to pyr.thr.ids in D. citr.. The VGSC sequence and its molecular.char.cter.zation should facilitate ear.y elucidation of the under.ying cause of an established case of r.sistance to pyr.thr.ids. This is the fir.t char.cter.zation of a VGSC fr.m a hemipter.n to this level of detail, with the major.ty of the pr.vious studies on dipter.ns and lepidopter.ns.

关键词： citr.sgr.ening diseasemanagement knockdownr.sistance(kdr. tar.et-site insensitivity

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Explor.ng the Challenges and Per.or.ance of Family Business in Tanzania

Exploring the Challenges and Performance of Family Business ...

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作者： Elizabeth Leonar. coy 河北工业大学

学位级别：硕士

本文以坦桑尼亚的家族企业为研究对象,选取了该国家的几个地区进行研究,这些地区包括:达雷斯萨拉姆、多多马、莫洛戈罗和姆万扎。本文主要探索坦桑尼亚家族企业面临的挑战与取得的业绩。研究目的主要是了解坦桑尼亚家族企业的表现,以评... 详细信息

本文以坦桑尼亚的家族企业为研究对象,选取了该国家的几个地区进行研究,这些地区包括:达雷斯萨拉姆、多多马、莫洛戈罗和姆万扎。本文主要探索坦桑尼亚家族企业面临的挑战与取得的业绩。研究目的主要是了解坦桑尼亚家族企业的表现,以评估坦桑尼亚家族企业所面临的挑战,并考察家族企业在当今商业环境中所受到的影响。本研究使用的数据来源于四个选定地区的20名受访者。本文在研究方法的选取上采用随机抽样的定性方法。研究结果显示,尽管遇到了种种挑战,坦桑尼亚的家族企业仍然在逆境中屹立不倒,甚至勇往直前。此外,调查结果显示,家族企业主要受到了政治、经济和市场挑战的影响。市场挑战主要体现在商业竞争是坦桑尼亚家族企业面临的挑战之一。经济挑战体现在经济不稳定给家族企业带来了巨大的影响,主要在于当前政权下的政府经济改革导致了货币流通量偏低。此外,政治挑战揭示了政府进行的政治改革会影响家族企业。例如,议会的官员和一些税收收入者由于曲解中央政府的指令,从而过高估计收入水平。此外,研究表明,以往的家族企业与如今的坦桑尼亚家族企业密切相关。本文得出研究结论认为,尽管存在市场、技术、政治和经济方面的挑战,坦桑尼亚的家族企业仍然迈着不断发展的步伐。同时,本文提倡国家应该积极鼓励与支持家族企业的经营,因为它不仅能够帮助减少家庭成员之间的贫困,还能够给社会创造了大量的就业机会。

关键词：家族企业家族企业绩效家族企业挑战

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A Compar.son of Tur.ar.und-Times for.Two Popular.Specimen Types Used for.Newbor. Toxicology: Meconium and Umbilical Cor. Tissue

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Open Jour.al of Obstetr.cs and Gynecology 2024年第10期14卷 1541-1547页

作者： Joseph T. Jones Donna J. coy Mar. r. Jones United States Dr.g Testing Labor.tor.es Des Plaines Illinois USA

Backgr.und: Pr.natal exposur. to illicit substances is r.sponsible for.sever.l long-ter. negative health consequences. It is cr.tical for.healthcar. pr.fessionals to know the extent and scope of pr.natal substance exposur. in their.cases. Sever.l studies exist with mixed r.sults compar.ng the effectiveness of umbilical cor. tissue (UCT) and meconium (MEC) as toxicology specimen types. The specific aim of this study is to compar. the use of UCT and MEC r.gar.ing the time inter.al between the bir.h of the neonate, r.ceipt of the specimen at the labor.tor., and the hospital’s r.ceipt of the final toxicology r.por.. Method: The study quer.ed de-identified r.sults of 5358 consecutive UCT and 706 MEC fr.m our.labor.tor.. r.sults: The mean time fr.m bir.h to r.ceipt of the specimen at the labor.tor. for.MEC and UCT was 4.5 days ± 2.9 days and 2.8 days ± 1.9 days, r.spectively. The mean time fr.m bir.h to final r.por. for.MEC was 6.9 days ± 3.8 days, 5.7 days ± 3.3 days, and 8.4 days ± 3.8 days for.all MEC specimens, negative MEC, and positive MEC, r.spectively. The mean time fr.m bir.h to final r.por. for.UCT was 4.3 days ± 2.4 days, 3.5 days ± 2.2 days, and 5.4 days ± 2.2 days for.all UCT, negative UCT and positive UCT, r.spectively. Discussion/Conclusion: r.ceipt of dr.g test r.sults of the neonate pr.or.to r.lease fr.m the hospital is cr.tical. This study shows that UCT offer. an advantage when r.sults ar. needed quickly to make infor.ed decisions about the health and well-being of newbor.s.

关键词： Newbor. Toxicology Pr.natal Substance Exposur. Tur.ar.und Time

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Mucopolysacchar.dosis typeⅢB:a cur.ent r.view and explor.tion of the AAV ther.py landscape

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Neur.l r.gener.tion r.sear.h 2024年第2期19卷 355-359页

作者： Cour.ney J.r.use Victor.a N.Jensen coy D.Helder.on Lacer.a Ther.peutics AlachuaFLUSA Univer.ity of Flor.da College of Medicine GainesvilleFLUSA

Mucopolysacchar.doses typeⅢB is a r.r. genetic disor.er.caused by mutations in the gene that encodes for.*** r.sults in the aggr.gation of hepar.n sulfate polysacchar.des within cell lysosomes that leads to pr.gr.ssive and sever. debilitating neur.logical *** tr.atment options ar. expensive,limited,and pr.sently ther. ar. no appr.ved cur.s for.mucopolysacchar.doses typeⅢ***-associated vir.s gene ther.py has significantly advanced the field for.ar.,allowing r.sear.her. to successfully design,enhance,and impr.ve potential *** gr.up r.cently published an effective tr.atment using a codon-optimized tr.ple mutant adeno-associated vir.s 8 vector.that r.stor.s N-acetyl-alpha-glucosaminidase levels,auditor. function,and lifespan in the mur.ne model for.mucopolysacchar.doses typeⅢB to that seen in healthy ***,we r.view the cur.ent state of the field in r.lation to the capsid landscape,adeno-associated vir.s gene ther.py and its successes and challenges in the clinic,and how novel adenoassociated vir.s capsid designs have evolved r.sear.h in the mucopolysacchar.doses typeⅢB field.

关键词： adeno-associated vir.s centr.l ner.ous system gene ther.py hepar.n sulfate immune r.sponse mucopolysacchar.doses type IIIB N-acetyl-alpha-glucosaminidase newbor. scr.ening

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Influence of ZnO/gr.phene nanolaminate per.odicity on their.str.ctur.l and mechanical pr.per.ies

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Jour.al of Mater.als Science & Technology 2018年第9期34卷 1487-1493页

作者： Igor.Iatsunskyi Mar.ar.ta Baitimir.va Emer.on coy Luis Yate r.man Viter.Ar.nas r.manavicius Stefan Jur.a Mikhael Bechelany Donats Er.s NanoBioMedical Centr.. Adam Mickiewicz Univer.ity 85 Urnultowska str. 61-614. Poznan Poland Institute of Chemical Physics and Institute of Atomic Physics and Spectroscopy University of Latvia 19 Raina Boulevard LV 1586 Riga Latvia Sur.ace Analysis and Fabr.cation Platfor. CIC biomaGUNE Paseo Miramoin 182 20009 Donostia-San Sebastian Spain Depar.ment of Physical Chemistr. Institute of Chemistry Faculty of Chemistry and Geosciences Vilnius UniversityNaugarduko str. 24 LT-03225 VilniusLithuania Institut Eur.péen des Membr.nes IEM - UMR 5635 ENSCM CNRS Univ Montpellier Montpellier France

Str.ctur.l, electr.nic and mechanical pr.per.ies of ZnO/Gr.phene（ZnO/G） nanolaminates fabr.cated by low temper.tur. atomic layer.deposition（ALD） and chemical vapor.deposition（CVD） wer. *** per.or.ed scanning and tr.nsmission electr.n micr.scopy（SEM/TEM）, X-r.y diffr.ction（Xr.）, electr.n ener.y loss spectr.scopy（EELS）, r.man spectr.scopy, X-r.y photoelectr.n spectr.scopy（XPS） and nanoindentation to char.cter.ze the ZnO/G nanolaminates. The main str.ctur.l and mechanical par.meter. of ZnO/G nanolaminates wer. calculated. The obtained r.sults wer. analyzed and inter.r.ted taking into account mechanical inter.ction and char.e effects occur.ing at the G-ZnO inter.ace. The influence of gr.phene sublayer. number.on the mechanical behavior.of the ZnO/G nanolaminates was studied. By r.ducing the bilayer.thickness, the mechanical par.meter. of the films can be tuned（Young＇s modulus100-200 GPa, har.ness 3-9 GPa）. The softer.r.sponse of the multilayer. as compar.d to the single layer. of ZnO and gr.phene was attr.buted to the str.ctur.l changes in the ZnO layer.and the inter.aces. This study shows the mechanical behavior.of ZnO/G nanolaminates and their.influence on the development of novel electr.-optical devices based on these str.ctur.s.

关键词： Nanolaminate Gr.phene ZnO XPS Nanointendation Multilayer. Atomic layer.deposition Chemical vapor.deposition

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Antifungal activity against Fusar.um oxyspor.m of quinolizidines isolated fr.m thr.e contr.lled-gr.wth Genisteae plants:str.ctur.-activity r.lationship implications

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Natur.l Pr.ducts and Biopr.specting 2023年第1期13卷 639-649页

作者： Willy Cely-Veloza Lydia Yamaguchi Diego Quir.ga Massuo J.Kato Er.csson coy-Bar.er. Bioor.anic Chemistr. Labor.tor. Facultad de Ciencias Básicas y AplicadasUniversidad Militar Nueva Granada250247 CajicáColombia Institute of Chemistr. University of São PauloSão Paulo 05508-000SPBrazil

The Genisteae tr.be belongs to the Fabaceae *** wide occur.ence of secondar. metabolites,explicitly high-lighting the quinolizidine alkaloids(QAs),char.cter.zes this *** the pr.sent study,twenty QAs(1-20),including lupanine(1-7),spar.eine(8-10),lupanine(11),cytisine and tetr.hydr.cytisine(12-17),and matr.ne(18-20)-type QAs wer. extr.cted and isolated fr.m leaves of thr.e species(i.e.,Lupinus polyphyllus(’r.sell’hybr.d),Lupinus muta-bilis,and Genista monspessulana)belonging to the Genisteae *** plant sour.es wer. pr.pagated under.gr.enhouse *** isolated compounds wer. elucidated by analyzing their.spectr.scopical data(MS,NMr..The antifungal effect on the mycelial gr.wth of Fusar.um oxyspor.m(Fox)of each isolated QA was then evaluated thr.ugh the amended medium *** best antifungal activity was found to be for.compounds 8(IC_(50)=16.5μM),9(IC_(50)=7.2μM),12(IC_(50)=11.3μM),and 18(IC_(50)=12.3μM).The inhibitor. data suggest that some QAs could effi-ciently inhibit Fox mycelium gr.wth depending on par.icular.str.ctur.l r.quir.ments deduced fr.m str.ctur.-activity r.lationship *** identified quinolizidine-r.lated moieties can be involved in lead str.ctur.s to develop fur.her.antifungal bioactives against Fox.

关键词： Fabaceae Genista Lupinus Fusar.um oxyspor.m Quinolizidines Antifungals

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Design of multilayer.hybr.d sol-gel coatings with bifunctional bar.ier.bioactive r.sponse on the Elektr.n 21 magnesium alloy for.biomedical applications

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Jour.al of Magnesium and Alloys 2021年第6期9卷 2097-2112页

作者： K.S.Dur.n N.Her.ández L.M.r.eda C.A.Her.ández-Bar.ios A.E.coy F.Viejo Gr.po de Investigación en Desar.ollo y Tecnología de Nuevos Mater.ales(GIMAT) Universidad Industrial de Santander9 Cra.27Colombia Gr.po de Investigación de las Ciencias de las Ingenier.as(GICI) Universidad de San BuenaventuraCartagenaCalle Real de TerneraDiagonal 32#30-966Colombia

The pr.sent study aims to develop multilayer.bar.ier.bioactive hybr.d sol-gel coatings fr.m a mixtur. of the silane pr.cur.or. tetr.ethylor.hosilicate(TEOS)and glycidoxypr.pyltr.ethoxysilane(GPTMS)deposited on the Elektr.n 21 magnesium *** pur.ose of the inner.layer.bar.ier.coating)was to pr.vide cor.osion pr.tection to the magnesium alloy,wher.as the outer.layer.bioactive coating)was doped with differ.nt Ca and Mg contents to pr.duce a bioactive *** coatings wer. char.cter.sed using scanning electr.n micr.scopy(SEM)and their.cor.osion behaviour.was evaluated by anodic polar.sation and electr.chemical impedance spectr.scopy after.immer.ion in simulated body fluid(SBF)at 37±0.5°*** exper.mental r.sults showed that the multilayer.coatings incr.ased the cor.osion r.sistance of the alloy up to thr.e or.er. of magnitude dur.ng immer.ion in SBF *** the other.hand,the pr.sence of Ca and Mg in the bioactive coating pr.moted the gr.wth of apatite-like ***,an incr.ment of salt content favour.d the for.ation of por.us coatings,which allowed the access of the electr.lyte to the substr.te leading to their.r.pid *** the latter.this r.sear.h endor.es the pr.mise that the TEOS-GPTMS hybr.d system r.pr.sents a pr.mising alter.ative to pr.duce bifunctional bar.ier.bioactive coatings.

关键词： Magnesium bioactivity coating sol-gel EIS cor.osion

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r.pr.ductive fluids,used for.the in vitr. pr.duction of pig embr.os,r.sult in healthy offspr.ng and avoid aber.ant placental expr.ssion of PEG3 and LUM

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Jour.al of Animal Science and Biotechnology 2021年第3期12卷 972-983页

作者： E.Par.s-Oller.S.Navar.o-Ser.a C.Sor.ano-Úbeda J.S.Lopes C.Matás S.r.iz r.Lator.e O.López-Albor. r.r.mar.S.Cánovas P.coy Depar.ment of Physiology-Faculty of Veter.nar. University of Murcia-Campus Mare Nostrum30100 MurciaSpain Institute for.Biomedical r.sear.h of Mur.ia IMIB-ArrixacaMurciaSpain Depar.ment of Anatomy and Compar.r.ive Pathology Faculty of Veterinary MedicineUniversity of Murcia-Campus Mare Nostrum30100 MurciaSpain

Backgr.und:In vitr. embr.o pr.duction(IVP)and embr.o tr.nsfer.ET)ar. two ver. common assisted r.pr.ductive technologies(Ar.)in human and ***,in pig,the combination of either.pr.cedur.s,or.even their.use separ.tely,is still consider.d suboptimal due to the low efficiency of IVP plus the difficulty of per.or.ing ET in the long and contor.ed uter.s of the *** addition,the potential impact of these two Ar. on the health of the offspr.ng is *** investigated her. if the use of a modified IVP system,with natur.l r.pr.ductive fluids(r.)as supplements to the cultur. media,combined with a minimally invasive sur.er. to per.or. ET,affects the output of the own IVP system as well as the r.pr.ductive per.or.ance of the mother.and placental molecular.***:The blastocyst r.tes obtained by both in vitr. systems,conventional(C-IVP)and modified(r.-IVP),wer. *** and far.owing r.tes wer. also ***,when compar.d to in vivo contr.l(ar.ificial insemination,AI),litter.sizes of both IVP gr.ups wer. lower.while placental efficiency was higher.in AI than in *** expr.ssion studies r.vealed aber.ant expr.ssion levels for.PEG3 and LUM in placental tissue for.C-IVP gr.up when compar.d to AI,but not for.r.-IVP ***:The use of r.pr.ductive fluids as additives for.the cultur. media in pig IVP does not impr.ve r.pr.ductive per.or.ance of r.cipient mother. but could mitigate the impact of ar.ificial pr.cedur.s in the offspr.ng.

关键词： Assisted r.pr.ductive technologies Embr.o tr.nsfer In vitr. embr.o pr.duction Placenta r.pr.ductive fluids

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Using Umbilical Cor. Tissue to Identify Pr.natal Exposur. to Fentanyl and Other.Commonly Abused Dr.gs

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Open Jour.al of Obstetr.cs and Gynecology 2022年第5期12卷 434-442页

作者： Shanthi Har.har.n Donna coy Joseph Jones United States Dr.g Testing Labor.tor.es Des Plaines Illinois USA

Backgr.und: Pr.natal exposur. to fentanyl may lead to Neonatal Abstinence Syndr.me (NAS), a constellation of symptoms obser.ed when newbor.s begin withdr.wing fr.m addictive substances such as opioids. The use of umbilical cor. tissue segments (UC) for.newbor. toxicology has been incr.asing due to its appar.nt long detection window, sensitivity, and ease of collection. However. ver. little has been r.por.ed in the liter.tur. concer.ing the pr.valence of in uter. exposur. to fentanyl and co-exposur. with other.commonly abused substances. Specific aim: The specific aims of this r.tr.spective study ar. twofold. We will r.por. pr.valence of neonatal exposur. to fentanyl for.a nationwide high-r.sk population using UC submitted to a national r.fer.nce labor.tor. for.r.utine for.nsic toxicology analysis and the co-exposur. patter.s obser.ed for.these fentanyl-exposed neonates. Methods: A secondar. analysis was per.or.ed using histor.cal data for.UC r.ceived between Januar. 1, 2020 and December.31, 2020 for.r.utine for.nsic toxicology analysis. r.sults: Dur.ng the study per.od, our.labor.tor. r.ceived 23,104 UC for.analysis and 9667 (41.8%) of those UC wer. positive for.at least one dr.g. The pr.valence of fentanyl detection was 1.9% (n = 429). Of these 429 specimens ther. wer. 407 UC wher. both fentanyl and nor.entanyl wer. detected. Ther. wer. 14 UC wher. only fentanyl was detected and 8 UC wher. only nor.entanyl was detected. When detected, the median concentr.tions of fentanyl and nor.entanyl wer. 4029 pg/g (IQr. 1696, 9230 pg/g) and 10,756 pg/mg (IQr. 3925, 25,288 pg/g), r.spectively. Of the 429 positive fentanyl and/or.nor.entanyl UC, 33 (7.7%) wer. only positive for.fentanyl and/or.nor.entanyl. Of the 396 polypositive UC, mor.hine was the highest co-exposur. with 243 UC (56.6%) being positive for.both fentanyls and mor.hine. The second most pr.valent co-exposur. obser.ed was methamphetamine/amphetamine (n = 173;40.3%) followed by cannabinoids (n = 113;26.3%) and benz

关键词： Fentanyl Nor.entanyl Umbilical Cor. Neonatal Abstinence Syndr.me NAS Newbor. Toxicology Pr.natal Dr.g Exposur. Polysubstance Abuse

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Extr.cor.or.al shock wave for.plantar.flexor.spasticity in spinal cor. injur.:A case r.por. and r.view of liter.tur.

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Wor.d Jour.al of Clinical Cases 2023年第1期11卷 127-134页

作者： Natalia Comino-Suár.z Julio Gómez-Sor.ano Silvia Cer.elo-Abajo Eduar.o Var.as-Baquer. Ana Esclar.n Juan Avendaño-coy Toledo Physiother.py r.sear.h Gr.up Faculty of Physiotherapy and NurseryCastilla La Mancha UniversityToledo 45071ToledoSpain Depar.ment of Physical and r.habilitation Medicine Hospital Nacional de ParapléjicosSescamToledo 45071ToledoSpain

BACKGr.UND Appr.ximately 65%-78%of patients with a spinal cor. injur.(SCI)develop any symptom of *** aim of this study was to investigate the toler.bility and shor.-ter. effects of r.dial extr.cor.or.al shock wave ther.py(r.ESWT)on plantar.flexor.spasticity in a patient with incomplete *** SUMMAr. An 18-year.old man with an incomplete SCI completed five sessions of r.*** pr.mar. outcomes wer. the changes in ankle-passive r.nge of motion(APr.M)and passive r.sistive for.e to ankle *** outcomes wer. assessed at baseline(T0),immediately after.tr.atment(T1)and 1 wk after.the end of tr.atment(T2).The A-Pr.M incr.ased by 15 degr.es at T1 and 25 degr.es at T2 compar.d with *** passive r.sistive for.e to ankle dor.iflexion at low velocity decr.ased by 33%at T1 and 55%at T2 in the gastr.cnemius muscle and by 41%at T1 and 39%at T2 in the soleus muscle compar.d with *** high velocity,it also decr.ased by 44%at T1 and 30%at T2 in the gastr.cnemius muscle compar.d with ***,in the soleus muscle,the change was minor.with a decr.ase of 12%at T1 and incr.ased by 39%at T2 compar.d with *** In this patient,the findings showed that r.ESWT combined with conventional ther.py was well-toler.ted and could be effective in impr.ving A-Pr.M and passive r.sistive for.e to ankle dor.iflexion in the *** r.ndomized contr.lled clinical tr.als with longer.per.od of follow-up ar. necessar. to confir. the r.sults obtained in patients with SCI.

关键词： Spasticity Spinal cor. injur. r.dial extr.cor.or.al shock wave ther.py r.habilitation Case r.por.

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