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Systematic mutational analysis of epitope-grafted ED3 immunogenicity reveals a DENV3-DENV4 bi-serospecific ED3 mutant

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Asian Pacific Journal of Tropical medicine 2022年第2期15卷 63-70,I0001-I0004页

作者： mamtaz Sultana Nazmul Hasan mamunur R.mahib mohammad m.islam Department of Biochemistry and molecular Biology University of ChittagongChittagong-4331Bangladesh

Objective:To identify the residue determinants of the serospecificity and sero-cross-reactivity of dengue virus(DENV)envelope protein domain 3(ED3),which contains two major putative epitopes of ***:We constructed ED3 from DENV3(3ED3)and DENV4(4ED3),and six epitope-grafted variants,where we transferred epitope 1(L304I,K305D,V309m,and S310A)and/or epitope 2(D383N,K384S,K387T,and N389H)of 4ED3 onto 3ED3 and *** albino mice aged 3-4 weeks were immunized against wildtype and epitope-grafted ED3 variants and anti-ED3 IgG antibody responses were determined using ***:mouse immunization using 3ED3 and 4ED3 generated serotype-specific antisera,as ***,most epitope-grafted ED3s produced antisera serospecific to the template ED3 with little or no cross-recognition of ED3 of the serotype from which the epitopes were *** indicated that a mere grafting of the epitope was not sufficient to transfer serospecificity,contrary to our ***,one epitope-grafted ED3 mutant,where epitope 1 of 3ED3 was grafted onto 4ED3(4ED3^(epi1)),generated antisera that was serospecific to both 4ED3 and ***:The 4ED3^(epi1)is a chimeric ED3 that produces antisera possessing serospecificity to both 3ED3 and 4ED3 onto a common 4ED3 *** 4ED3^(epi1),therefore,provides a unique tool for analyzing serospecificity and sero-cross-reactivity in *** believe that chimeric ED3 may provide a template for future recombinant ED3 possessing serospecificity of multiple DENVs onto a single scaffold and may pave a way developing tri-and/or tetravalent anti-DENV antisera.

关键词： Dengue virus serospecificity Sero-cross-recognition Anti-ED3 antibodies Epitope-grafting Chimeric ED3

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multi-level supervisory emergency control for operation of remote area microgrid clusters

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Journal of modern Power Systems and Clean Energy 2019年第5期7卷 1210-1228页

作者： munira BATOOL Farhad SHAHNIA Syed m.islam School of Electrical Engineering Computing and Mathematical SciencesCurtin UniversityPerthAustralia School of Engineering and Information Technology Murdoch UniversityPerthAustralia School of Science Engineering and Information TechnologyFederation UniversityBallaratAustralia

Remote and regional areas are usually supplied by isolated and self-sufficient electricity systems,which are called as microgrids(mGs).To reduce the overall cost of electricity production,mGs rely on non-dispatchable renewable *** such as overloading or excessive generation by renewable sources can result in a substantial voltage or frequency deviation in *** paper presents a supervisory controller for such *** key idea is to remedy the emergencies by optimal internal or external support.A multi-level controller with soft,intermedial and hard actions is *** soft actions include the adjustment of the droop parameters of the sources and the controlling of the charge/discharge of energy *** intermedial action is exchanging power with neighboring mGs,which is highly probable in large remote *** the last remedying resort,curtailing loads or renewable sources are assumed as hard *** proposed controller employs an optimization technique consisting of certain objectives such as reducing power loss in the tie-lines amongst mGs and the dependency of an mG to other mGs,as well as enhancing the contribution of renewable sources in electricity *** of the fuel consumption and emissions of conventional generators,along with frequency and voltage deviation,is the other desired *** performance of the proposal is evaluated by several numerical analyses in mATLAB~?.

关键词： microgrids Emergency controller Optimization Remote area

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Dynamic response in two-dimensional transversely isotropic thick plate with spatially varying heat sources and body forces

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Applied mathematics and mechanics(English Edition) 2011年第10期32卷 1315-1332页

作者： m.islam S.H.mALLIK m.KANORIA Department of mathematics St. Xavier's College (Evening) Kolkata 700016 India Department of mathematics Aliah University Kolkata 700091 India Department of Applied mathematics University of Calcutta Kolkata 700009 India

This paper deals with a two-dimensional （2D） problem for a transverselyisotropic thick plate having heat sources and body forces. The upper surface of the plate is stress free with the prescribed surface temperature, while the lower surface of the plate rests on a rigid foundation and is thermally insulated. The study is carried out in the context of the generalized thermoelasticity proposed by Green and Naghdi. The governing equations for displacement and temperature fields are obtained in the Laplace-Fourier transform domain by applying the Laplace and Fourier transforms. The inversion of the double transform is done numerically. Numerical inversion of the Laplace transform is done based on the Fourier series expansion. Numerical computations are carried out for magnesium （mg）, and the results are presented graphically. The results for an isotropic material （Cu） are obtained numerically and presented graphically to be compared with those of a transversely isotropic material （mg）. The effect of the body forces is also studied.

关键词： generalized thermoelasticity Green-Naghdi model transversely isotropic material spatially varying heat source body force

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Genetic diversity and association mapping for salinity tolerance in Bangladeshi rice landraces

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The Crop Journal 2015年第5期3卷 440-444页

作者： Reza m.Emon mirza m.islam Jyotirmoy Halder Yeyang Fan Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology China National Rice Research Institute Bangladesh Institute of Nuclear Agriculture

Breeding for salinity tolerance using Bangladeshi rice landraces and understand genetic diversity has been limited by the complex and polygenic nature of salt tolerance in rice genotypes. A genetic diversity and association mapping analysis was conducted using 96 germplasm accessions with variable response to salt stress at the seedling stage. These included86 landraces and 10 indica varieties and lines including Nona Bokra, from southern Bangladesh. A total of 220 alleles were detected at 58 Simple Sequence Repeat(SSR) marker loci randomly distributed on all 12 rice chromosomes and 8 Sequence Tagged Site(STS) markers developed for genes SKC1, DST, and SalT. The average gene diversity was 0.5075 and polymorphism information content value was 0.4426, respectively. Cluster analysis revealed that 68 and 21 accessions were clustered into 2 distinct groups, possibly corresponding to indica and japonica groups, respectively and the remaining 7 landraces were classified as an admixed group. In addition to Wn11463, the STS marker for SKC1, Rm22418 on Chr. 8 was significantly associated with salinity tolerance, at the location of a QTL detected in previous studies. Our findings of favorable alleles associated with salinity tolerance in Bangladeshi rice landraces, as well as the development of STS markers for salt tolerance genes, will be helpful in future efforts to breed salinity tolerance in rice.

关键词： Oryza sativa L Salinity tolerance Seedling stage S

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Reduced tillage with residue retention improves soil labile carbon pools and carbon lability and management indices in a seven-year trial with wheat-mung bean-rice rotation

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Pedosphere 2022年第6期32卷 916-927页

作者： Rafeza BEGUm mohammad m.R.JAHANGIR mohammad JAHIRUDDIN mohammad Rafiqul islam Shaikh m.BOKHTIAR Khandakar R.islam Department of Soil Science Bangladesh Agricultural UniversityMymensingh 2202(Bangladesh) Soil Resource Development Institute Dhaka 1215(Bangladesh) South Asian Association for Regional Cooperation(SAARC)Agriculture Center Dhaka 1215(Bangladesh) The Ohio State University Columbus OH 43235(USA)

Soil total organic carbon(TOC)is a composite indicator of soil quality with implications for crop production and the regulation of soil ecosystem *** reports on the dynamics of TOC as a consequence of soil management practices in subtropical climatic conditions,where microbial carbon(C)loss is high,are very *** objective of our study was to evaluate the impact of seven years of continuous tillage and residue management on soil TOC dynamics(quantitative and qualitative)with respect to lability and stratification under an annual wheat-mung bean-rice cropping *** soil samples were collected at 0-15 and 15-30 cm depths from a three-replicate split-plot experiment with tillage treatment as the main plots and crop residue levels as the *** tillage treatments included conventional tillage(CT)and strip tillage(ST).Residue levels were high residue level(HR),30%of the plant height,and low residue level(LR),15%.In addition to TOC,soil samples were analyzed for particulate organic C(POC),permanganate oxidizable C(POXC),basal respiration(BR),specific maintenance respiration rate(qCO_(2)),microbial biomass C(mBC),potentially mineralizable C(PmC),and TOC lability and management *** ST treatment significantly increased the TOC and labile C pools at both depths compared with the CT treatment,with the effect being more pronounced in the surface *** HR treatment increased TOC and labile C pools compared with the LR *** ST+HR treatment showed significant increases in mBC,metabolic quotient(qR),C pool index(CPI),C lability index(CL_(I)),and C management index(CmI),indicating improved and efficient soil biological activities in such systems compared with the CT ***,the stratification values,a measure of soil quality improvement,for POC and mBC were>2,indicating improved soil quality in the ST+HR treatment compared with the CT *** ST+HR treatment not only significantly increased the contents o

关键词： basal respiration carbon management index carbon stratification particulate organic carbon permanganate oxidizable carbon total organic carbon

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The Two-Dimensional Supersonic Flow and mixing with a Perpendicular Injection in a Scramjet Combustor

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Journal of Thermal Science 2003年第4期12卷 371-380页

作者： mohammad Ali S. Ahmed A.K.m. Sadrul islam Department of mechanical Engineering Bangladesh University of Engineering and Technology (BUET) Dhaka-1000 Bangladesh.Department of Mechanical Engineering Bangladesh University of Engineering and Technology (BUET) Dhaka-1000 Bangladesh.Department of Mechanical Engineering Bangladesh University of Engineering and Technology (BUET) Dhaka-1000 Bangladesh.

A numerical investigation has been performed on supersonic mixing of hydrogen with air in a Scramjet (Supersonic Combustion Ramjet) combustor and its flame holding capability by solving Two-Dimensional full Navier-Stokes equations. The main flow is air entering through a finite width of inlet and gaseous hydrogen is injected perpendicularly from the side wall. An explicit Harten-Yee Non-mUSCL modified-flux-type TVD scheme has been used to solve the system of equations, and a zero-equation algebraic turbulence model to calculate the eddy viscosity coefficient. In this study the enhancement of mixing and good flame holding capability of a supersonic combustor have been investigated by varying the distance of injector position from left boundary keeping constant the backward-facing step height and other calculation parameters. The results show that the configuration for small distance of injector position has high mixing efficiency but the upstream recirculation can not evolved properly which is an important factor for flame holding capability. On the other hand, the configuration for very long distance has lower mixing efficiency due to lower gradient of hydrogen mass concentration on the top of injector caused by the expansion of side jet in both upstream and downstream of injector. For moderate distance of injector position, large and elongated upstream recirculation can evolve which might be activated as a good flame holder.

关键词： scramjet engine TVD scheme finite width of inlet supersonic mixing parallel air stream flame holding.

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The Effect of Injection Angle on mixing and Flame Holding in Supersonic Combustor

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Journal of Thermal Science 2002年第1期11卷 80-91页

作者： Shakil Ahmed mohammad Ali A. K. m. Sadrul islam Department of mechanical Engineering Bangladesh University of Engineering and Technology (BUET) Dhaka Bangladesh

A numerical study on mixing of hydrogen injected into a supersonic air stream has been performed by solving two-dimensional full Navier-Stokes equations. An explicit Harten-Yee Non-mUSCL modified-flux-type TVD scheme has been used to solve the system of equations, and a zero-equation algebraic turbulence model to calculate the eddy viscosity coefficient. The main objectives of this study are to increase the mixing efficiency and flame holding capability of a supersonic combustor. The performance of combustor has been investigated by varying the hydrogen injection angle made with the direction of air stream considering anti-clockwise direction as positive. The injector position from left boundary, backward-facing step height and the inlet width of air stream are kept constant. The results show that upstream of injector the mixing is dominated by recirculation and in downstream the mixing is dominated by mass concentration of hydrogen. Upstream recirculation is dominant for injecting angle 600 and 900 . Incorporating the various effects, perpendicular injection shows the maximum mixing efficiency and its large upstream recirculation region has a good flame holding capability.

关键词： scramjet engine TVD scheme backward-facing step supersonic mixing supersonic stream flame holding.

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Foliar Application of Cytokinin modulates Gas Exchange Features,Water Relation and Biochemical Responses to Improve Growth Performance of maize under Drought Stress

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Phyton-International Journal of Experimental Botany 2022年第3期91卷 633-649页

作者： m.Rafiqul islam m.Shahinur islam Nurunnaher Akter mohammed mohi-Ud-Din mohammad Golam mostofa Department of Agronomy Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Department of Crop Botany Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Department of Biochemistry and molecular Biology Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Institute of Genomics for Crop Abiotic Stress Tolerance Department of Plant and Soil ScienceTexas Tech UniversityLubbockTX 79409USA

Improvement of plant performance under drought stress is crucial to sustaining agricultural *** current study investigated the ameliorative effects of foliar-applied kinetin,an adenine-type cytokinin(CK),on growth and gas exchange parameters,water relations and biochemical attributes of maize plants under drought ***-day-old maize plants were subjected to drought by maintaining soil moisture content at 25%field capacity for 8 days followed by foliar application of kinetin at 0,75,150 and 225 mg L^(−1)(CK0,CK75,CK150 and CK225,respectively)to the plants for two-times at the 9-day *** revealed that drought stress markedly reduced stem diameter,dry weight,chlorophyll content,gas exchange parameters and water balance but increased proline,malondialdehyde and soluble sugar contents,electrolyte leakage and senescence in maize *** of exogenous CK remarkably improved maize performance by modulating growth,gas exchange-and water relation-related parameters in a dose-dependent manner under drought ***225 increased chlorophyll content(by 61.54%),relative water content(by 49.14%),net photosynthesis rate(by 39.94%)and transpiration rate(by 121.36%)and also delayed leaf senescence but decreased internal CO_(2)concentration(by 7.38%),water saturation deficit(by 40.40%)and water uptake capacity(by 42.49%)in both well-watered and droughtstressed ***,CK application considerably decreased electrolyte leakage,proline,malondialdehyde and soluble sugar levels in drought-stressed maize plants,as also supported by heatmap and cluster *** together,exogenous CK at proper concentration(225 mg L^(−1))successfully improved maize performance under drought conditions,thereby suggesting CK application as a useful approach to alleviate drought-induced adverse effects in maize plants,and perhaps in other important crop plants.

关键词： Cytokinin drought maize osmoprotection photosynthesis water-use-efficiency

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Predicting short-term adverse outcomes in the geriatric population presenting with syncope:a comparison of existing syncope rules and beyond

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Journal of Geriatric Cardiology 2023年第1期20卷 11-22页

作者： Suud A.Kiradoh Timothy E.Craven maria O.Rangel Lillian m.Nosow Erfan Zarrinkhoo Suma menon Parag A.Chevli Tareq m.islam Luqman A.Thazhatuveetil-Kunhahamed Department of Internal medicine Atrium Health Wake Forest Baptist Medical CenterWinston-SalemUSA Biostatistics EpidemiologyResearchand Design(BERD)Atrium Health Wake Forest Baptist Medical CenterWinston-SalemUSA Department of Cardiology Atrium Health Wake Forest Baptist Medical CenterUSA

OBJECTIVES Syncope at age 65+is associated with increased mortality,irrespective of *** rules were designed to aid in risk-stratification but were only validated in the general adult *** objective was to determine if they can be applied to a geriatric population in predicting short-term adverse *** In this single-center retrospective study,we evaluated 350 patients aged 65+presenting with *** criteria included confirmed non-syncope,active medical condition,drug or alcohol-related *** were stratified into high or low risk based on Canadian Syncope Risk Score(CSRS),Evaluation of Guidelines in Syncope Study(EGSYS),San Francisco Syncope Rule(SFSR),and Risk Stratification of Syncope in the Emergency Department(ROSE).Composite adverse outcomes at 48-hour and 30-day included all-cause mortality,major adverse cardiac and cerebrovascular events(mACCE),return emergency department visit,hospitalization,or medical *** assessed each score's ability to predict the outcomes using logistic-regression and compared performances using receiver-operator *** analyses were performed to study the associations between recorded parameters and *** CSRS outperformed with AUC of 0.732(95%CI:0.653-0.812)and 0.749(95%CI:0.688-0.809)for 48-h and 30-day outcomes,*** for CSRS,EGSYS,SFSR,and ROSE for 48-hour outcomes were 48%,65%,42% and 19%;and for 30-day outcomes were 72%,65%,30% and 55%,*** fibrillation/flutter on EKG,congestive heart failure,antiarrhythmics,systolic blood-pressuremality,heart disease history,severe pulmonary hypertension,BNP>300,vasovagal predisposition,and antidepressants highly correlated with 30-day *** Performance and accuracy of four prominent syncope rules were suboptimal in identifying high-risk geriatric patients with short-term

关键词： syncope mortality return

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Development of chemometric model for characterization of non-wood by FT-NIR data

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Journal of Bioresources and Bioproducts 2020年第3期5卷 196-203页

作者： mohammad Nashir Uddin Taslima Ferdous Zahidul islam m.Sarwar Jahan m.A.Quaiyyum BCSIR Laboratories DhakaBangladesh Council of Scientific and Industrial Research(BCSIR)Dhaka 1205Bangladesh Department of Applied Chemistry and Chemical Engineering University of DhakaDhaka 1000Bangladesh

In this study,a model for prediction of lignocellulose components of agricultural residues has been developed with Fourier Transformed Near Infrared(FT-NIR)spectroscopy *** calibration techniques(Principal Component Regression(PCR)and Partial Least Square Regression(PLSR))were assessed for prediction of lignin,holocellulose,α-cellulose,pentosan and ash,and found the PLSR better for lignin,holocellulose andα-*** PCR also produced better results for quantification of pentosan and *** range(7000-5000 cm^(-1))showed more informative than other parts of the spectral *** PLSR showed maximum value of R^(2)(R^(2)=0.91%)for prediction of *** the prediction of pentosan,the PCR was better(R^(2)=0.68%).The PCR also showed better results(R^(2)=86%)for quantification of *** determine amount of lignin,the PLSR was the best(R^(2)=0.83%)when the spectral data were de-trained and smoothed with Savitzky-Golay(S-G)filtering *** prediction ofα-cellulose,the PLSR was the best model(R^(2)=0.94%)when the data were pretreated with mean *** the best alternatives inNear Infrared(NIR)data preprocessing and calibration techniques,methods for quantification of lignocellulose components of agricultural residues have been developed which is rapid,cost effective,and less chemical intensive and easily usable in pulp and paper industries and pulp testing laboratories.

关键词： Agricultural residue multivariate modeling Lignocellulose component

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