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ATP binding cassette proteins ABCG37 and ABCG33 function as potassium-independent cesium uptake carriers in Arabidopsis roots

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Molecular Plant 2021年第4期14卷 664-678页

作者： mohammad arif ashraf Takashi Akihiro Keita Ito Sayaka Kumagai Ryohei Sugita Keitaro Tanoi Abidur Rahman United Graduate School of Agricultural Sciences Iwate UniversityMoriokaIwate 020-8550Japan Faculty of Lite and Environmental Science Shimane UniversityMatsueShimane 690-8504Japan Faculty of Agriculture Department of Plant Bio SciencesIwate UniversityMoriokaIwate 0208550Japan Graduate School of Agricultural and Life Sciences.The University of Tokyo Tokyo 113-8654.Japan PRESTO Japan Science and Technology Agency(JST)4-1-8 HonchoKawaguchiSaitama 332-0012Japan Agr-Innovation Iwate UniversityMoriokaIwate 020-8550Japan These authors contributed equally Present address:Biology Department University of MassachusettsAmherstMA USA

Radiocesium accumulated in the soil by nuclear accidents is a major environmental *** transport process of cesium(Cs^(+))is tightly linked to the indispensable plant nutrient potassium(K^(+))as they both belong to the group I alkali metals with similar chemical *** of the transporters that had been characterized to date as Cs*transporters are directly or indirectly linked to K^(+).Using a combinatorial approach of physiology,genetics,cell biology,and root uptake assay,here we identified two ATP-binding cassette(ABC)proteins,ABCG37 and ABCG33,as facilitators of Cs^(+)influx.A gain-of-function mutant of ABCG37(abcg37-1)showed increased sensitivity to Cs^(+)-induced root growth inhibition,while the double knockout mutant of ABCG33 and ABCG37(abcg33-1abcg37-2)showed resistance,whereas the single loss-of-function mutants of ABCG33 and ABCG37 did not show any alteration in Cs^(+)*** planta short-term radioactive Cs^(+)-uptake assay along with growth and uptake assays in a heterologous system confirmed ABCG33 and ABCG37 as Cs^(+)-uptake *** response and content were unaffected in the double-mutant background and yeast cells lacking potassium-uptake carriers transformed with ABCG33 and ABCG37 failed to grow in the absence of K^(+),confirming that Cs^(+)uptake by ABCG33 and ABCG37 is independent of K^(+).Collectively,this work identified two ABC proteins as new Cs^(+)-influx carriers that act redundantly and independent of the K^(+)-uptake pathway.

关键词： cesium transport ABC proteins uptake Arabidopsis root biology

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PIN FORMED 2 Modulates the Transport of Arsenite in Arabidopsis thaliana

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Plant Communications 2020年第3期1卷 42-56页

作者： mohammad arif ashraf Kana Umetsu Olena Ponomarenko Michiko Saito mohammad Aslam Olga Antipova Natalia Dolgova Cheyenne D.Kiani Susan Nehzati Keitaro Tanoi Katsuyuki Minegishi Kotaro Nagatsu Takehiro Kamiya Toru Fujiwara Christian Luschnig Karen Tanino Ingrid Pickering Graham N.George Abidur Rahman United Graduate School of Agricultural Sciences Iwate UniversityMoriokaIwateJapan Department of Plant Bio Sciences Faculty of AgricultureIwate UniversityMoriokaIwateJapan Molecular and Environmental Science Research Group Department of Geological SciencesUniversity of SaskatchewanSaskatoonSKCanada Argonne National Lab Advanced Photon SourceXSD-MICLemontILUSA Isotope Facility for Agricultural Education and Research Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuTokyoJapan Department of Radiopharmaceuticals Development National Institute of Radiological SciencesNational Institutes for Quantum and Radiological Science and TechnologyInageChibaJapan Department of Applied Biological Chemistry Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuTokyoJapan Department of Applied Genetics and Cell Biology University of Natural Resources and Life SciencesVienna(BOKU)Muthgasse 181180 WienAustria Department of Plant Sciences College of Agriculture and BioresourcesUniversity of SaskatchewanSaskatoonSKCanada Agri-Innovation Center Iwate UniversityMoriokaIwateJapan

Arsenic contamination is a major environmental issue,as it may lead to serious health *** reduced trivalent formof inorganic arsenic,arsenite,is in generalmore toxic to plants comparedwith the fully oxidized pentavalent *** arsenite inplants hasbeenshown tobemediatedthrough a large subfamily of plant aquaglyceroporins,nodulin 26-like intrinsic proteins(NIPs).However,the efflux mechanisms,as well as themechanismof arsenite-induced root growth inhibition,remain poorly *** physiology,synchrotron imaging,and root transport assay approaches,we show that the cellular transport of trivalent arsenicals inArabidopsis thalianais stronglymodulatedbyPINFORMED2(PIN2)auxin efflux *** transport assay using radioactive arsenite,X-ray fluorescence imaging(XFI)coupled with X-ray absorption spectroscopy(XAS),and inductively coupled plasma mass spectrometry analysis revealed that pin2 plants accumulate higher concentrations of arsenite in roots comparedwith the *** the cellular level,arsenite specifically targets intracellular sorting of PIN2 and thereby alters the cellular auxin ***,loss of PIN2 function results in arsenite hypersensitivity in *** coupled with XAS further revealed that loss of PIN2 function results in specific accumulation of arsenical species,but not the other metals such as iron,zinc,or calcium in the root ***,these results suggest that PIN2 likely functions as an arsenite efflux transporter for the distribution of arsenical species in planta.

关键词： auxin arsenite PIN2 trafficking transport

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Enhancing Deepfake Detection:Proactive Forensics Techniques Using Digital Watermarking

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Computers, Materials & Continua 2025年第1期82卷 73-102页

作者： Zhimao Lai Saad arif Cong Feng Guangjun Liao Chuntao Wang School of Automation Guangdong University and TechnologyGuangzhou510006China School of Immigration Administration(Guangzhou) China People’s Police UniversityGuangzhou510663China Department of Mechanical Engineering College of EngineeringKing Faisal UniversityAl Ahsa31982Saudi Arabia Cyber Police Division Guangzhou Public Security BureauGuangzhou510030China School of Forensic Science and Technology Guangdong Police CollegeGuangzhou510320China College of Mathematics and Informatics South China Agricultural UniversityGuangzhou510642China

With the rapid advancement of visual generative models such as Generative Adversarial Networks(GANs)and stable Diffusion,the creation of highly realistic Deepfake through automated forgery has significantly *** paper examines the advancements inDeepfake detection and defense technologies,emphasizing the shift from passive detection methods to proactive digital watermarking *** detection methods,which involve extracting features from images or videos to identify forgeries,encounter challenges such as poor performance against unknown manipulation techniques and susceptibility to counter-forensic *** contrast,proactive digital watermarking techniques embed specificmarkers into images or videos,facilitating real-time detection and traceability,thereby providing a preemptive defense againstDeepfake *** offer a comprehensive analysis of digitalwatermarking-based forensic techniques,discussing their advantages over passivemethods and highlighting four key benefits:real-time detection,embedded defense,resistance to tampering,and provision of legal ***,the paper identifies gaps in the literature concerning proactive forensic techniques and suggests future research directions,including cross-domain watermarking and adaptive watermarking *** systematically classifying and comparing existing techniques,this review aims to contribute valuable insights for the development of more effective proactive defense strategies in Deepfake forensics.

关键词： Deepfake proactive forensics digital watermarking traceability detection techniques

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Review of Techniques for Integrating Security in Software Development Lifecycle

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Computers, Materials & Continua 2025年第1期82卷 139-172页

作者： Hassan Saeed Imran Shafi Jamil Ahmad Adnan Ahmed Khan Tahir Khurshaid Imran ashraf College of Electrical and Mechanical Engineering National University of Sciences and Technology(NUST)Rawalpindi46604Pakistan Department of Computer Science Abasyn University Islamabad CampusIslamabad45510Pakistan Military College of Signals National University of Sciences and Technology(NUST)Rawalpindi46600Pakistan Department of Electrical Engineering Yeungnam UniversityGyeongsan38541Republic of Korea Department of Information and Communication Engineering Yeungnam UniversityGyeongsan38541Republic of Korea

Software-related security aspects are a growing and legitimate concern,especially with 5G data available just at our *** conduct research in this field,periodic comparative analysis is needed with the new techniques coming up *** purpose of this study is to review the recent developments in the field of security integration in the software development lifecycle(SDLC)by analyzing the articles published in the last two decades and to propose a way *** review follows Kitchenham’s review *** review has been divided into three main stages including planning,execution,and *** the selected 100 articles,it becomes evident that need of a collaborative approach is necessary for addressing critical software security risks(CSSRs)through effective risk management/estimation *** risks using a numeric scale enables a comprehensive understanding of their severity,facilitating focused resource allocation and mitigation *** a comprehensive understanding of potential vulnerabilities and proactive mitigation efforts facilitated by protection poker,organizations can prioritize resources effectively to ensure the successful outcome of projects and initiatives in today’s dynamic threat *** review reveals that threat analysis and security testing are needed to develop automated tools for the *** estimation of effort required to prioritize potential security risks is a big challenge in software *** accuracy of effort estimation can be further improved by exploring new techniques,particularly those involving deep *** is also imperative to validate these effort estimation methods to ensure all potential security threats are *** challenge is selecting the right model for each specific security *** achieve a comprehensive evaluation,researchers should use well-known benchmark checklists.

关键词： Software development lifecycle systematic literature review critical software security risks national institute of standards and technology DevSecOps open web application security project McGraw’s touch points

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Evolutionarily conserved mechanosensor PIEZO in land plants

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Molecular Plant 2021年第11期14卷 1783-1784页

作者： M.arif ashraf Department of Biology University of Massachusetts AmherstAmherstMAUSA

Mechanosensing is one of the fundamental cellular processes that helps cells respond to universal stimuli such as light,touch,gravity,and *** are the most well-characterized mechanosensors in mammals and can be activated by applying pressure from either a patch-clamp recording pipette or a blunt glass pipette,as the Greek word piezo means *** activation,the PIEZO channel facilitates the entry of Ca2+inside the cell(Figure 1)。

关键词： Figure patch recording

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An evolutionarily conserved nitrate sensor inplants

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Molecular Plant 2022年第11期15卷 1652-1653页

作者： M.arif ashraf Stavroula Fili Department of Biology University of Massachusetts AmherstAmherstMA 01003USA Plant Biology Program University of Massachusetts AmherstAmherstMA 01003USA

Nitrogen availability in plant tissues triggers transcriptomic responses that affect not only nitrate homeostasis but also metabolic,hormonal,and developmental processes(Wang et al.,2018).The ability to sense extracellular nitrate has been attributed to the characterized nitrate transceptor NRT1.1(CHL1/NPF6.3);however,null chl1-5 mutants are not entirely impaired in the primary nitrogen response,suggesting that there might be other sensors that act independently(Wang et al.,2018).Furthermore,the calcium-sensor protein kinases(CPKs)responsible for transducing nitrate signaling downstream,even when constitutively activated,still require the presence of nitrate(Liu et al.,2017),indicating an additional nitrate-sensing mechanism.

关键词： mechanism nitrate entirely

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Pineapple SWEET10 is a glucose transporter

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Horticulture Research 2023年第10期10卷 74-87页

作者： Beenish Fakher M.arif ashraf Lulu Wang Xiaomei Wang Ping Zheng mohammad Aslam Yuan Qin State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi Key Lab of Sugarcane BiologyCollege of AgricultureGuangxi UniversityNanning 530004GuangxiChina College of Life Sciences Key Laboratory of GeneticsBreeding and Multiple Utilization of CropsMinistry of EducationFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhouFujian 350002China Department of Biology Howard UniversityWashington DC 20059USA Horticulture Research Institute Guangxi Academy of Agricultural SciencesNanning Investigation Station of South Subtropical Fruit TreesMinistry of AgricultureNanning 530004China Donald Danforth Plant Science Center Saint LouisMO 63132USA

SWEET transporters are a unique class of sugar transporters that play vital roles in various developmental and physiological processes in *** the functions of SWEETs have been well established in model plants such as Arabidopsis,their functions in economically important fruit crops like pineapple have not been well *** we aimed to investigate the substrate specificity of pineapple SWEETs by comparing the protein sequences of known glucose and sucrose transporters in Arabidopsis with those in *** genome-wide approach and 3D structure comparison showed that the Arabidopsis SWEET8 homolog in pineapple,AcSWEET10,shares similar sequences and protein properties responsible for glucose *** determine the functional conservation of AcSWEET10,we tested its ability to complement glucose transport mutants in yeast and analyzed its expression in stamens and impact on the microspore phenotype and seed set in transgenic *** results showed that AcSWEET10 is functionally equivalent to AtSWEET8 and plays a critical role in regulating microspore formation through the regulation of the Callose synthase5(CalS5),which highlights the importance of SWEET transporters in *** information could have important implications for improving fruit crop yield and quality by manipulating SWEET transporter activity.

关键词： SWEET1 Arabidopsis sequences

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Secure Channel Estimation Using Norm Estimation Model for 5G Next Generation Wireless Networks

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Computers, Materials & Continua 2025年第1期82卷 1151-1169页

作者： Khalil Ullah Song Jian Muhammad Naeem Ul Hassan Suliman Khan mohammad Babar Arshad Ahmad Shafiq Ahmad Faculty of Information Engineering and Automation Kunming University of Science and TechnologyKunming650500China Department of Computer Science Abbottabad University of Science and TechnologyHavelianAbbottabad22500Pakistan Department of Computing and Electronics Engineering Middle East CollegeMuscat124Oman Department of CS&IT Pak-Austria Fachhochschule Institute of Applied Sciences and Technology MangHaripur22621Pakistan Industrial Engineering Department College of EngineeringKing Saud UniversityRiyadh11421Saudi Arabia

The emergence of next generation networks(NextG),including 5G and beyond,is reshaping the technological landscape of cellular and mobile *** networks are sufficiently scaled to interconnect billions of users and *** in academia and industry are focusing on technological advancements to achieve highspeed transmission,cell planning,and latency reduction to facilitate emerging applications such as virtual reality,the metaverse,smart cities,smart health,and autonomous *** continuously improves its network functionality to support these *** input multiple output(MIMO)technology offers spectral efficiency,dependability,and overall performance in *** article proposes a secure channel estimation technique in MIMO topology using a norm-estimation model to provide comprehensive insights into protecting NextG network components against adversarial *** technique aims to create long-lasting and secure NextG networks using this extended *** viability of MIMO applications and modern AI-driven methodologies to combat cybersecurity threats are explored in this ***,the proposed model demonstrates high performance in terms of reliability and accuracy,with a 20%reduction in the MalOut-RealOut-Diff metric compared to existing state-of-the-art techniques.

关键词： Next generation networks massive mimo communication network artificial intelligence 5G adversarial attacks channel estimation information security

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Engendered nanoparticles for treatment of brain tumors

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Oncology Research 2025年第1期33卷 15-26页

作者： SOROUSH SOLEYMANI mohammad DOROUDIAN MAHDIEH SOEZI ALI BELADI KIARASH ASGARI ASO MOBARAKSHAHI ARYANA AGHAEIPOUR RONAN MACLOUGHLIN Department of Cell and Molecular Biology School of Biological SciencesUniversity of LeicesterLeicesterLE17RHUK Department of Cell and Molecular Biology Faculty of Biological SciencesKharazmi UniversityTehran15719-14911Iran Cancer Epidemiology Research Center Aja University of Medical SciencesTehran14117-18541Iran Medical Biotechnology Research Center Aja University of Medical SciencesTehran14117-18541Iran Department of Life Sciences Faculty of Natural SciencesImperial College LondonLondonSW72AZUK School of Pharmacy and Biomolecular Sciences Royal College of Surgeons in Ireland(RCSI)DublinD02 YN77Ireland Research and Development Science and Emerging TechnologiesAerogen Ltd.Galway Business ParkGalwayH91 HE94Ireland School of Pharmacy and Pharmaceutical Sciences Trinity CollegeDublinD02 PN40Ireland

Brain metastasis and primary glioblastoma multiforme represent the most common and lethal malignant brain *** median survival time is typically less than a year after *** of the major challenges in treating these cancers is the efficiency of the transport of drugs to the central nervous *** blood-brain barrier is cooperating with advanced stages of *** blood-brain barrier poses a significant challenge to delivering systemic medications to brain *** delivery systems have emerged as promising tools for effectively crossing this ***,the development of smart nanoparticles brings new hope for cancer diagnosis and *** nanoparticles improve drug delivery efficiency,allowing for the creation of targeted and stimuli-responsive delivery *** review highlights recent advancements in nanoparticle and smart nanoparticle technologies for brain cancer treatment,exploring the range of nanoparticles under development,their applications,targeting strategies,and the latest progress in enhancing transport across the blood-brain *** also addresses the ongoing challenges and potential benefits of these innovative approaches.

关键词： Nanoparticles Smart nanoparticles Stimuli responsive nanoparticles Targeted nanoparticles Blood-brain barrier(BBB) Brain cancer

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Response of masonry systems against blast loading

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Defence Technology（防务技术） 2021年第4期17卷 1326-1337页

作者： Eid Badshah Amjad Naseer mohammad ashraf Tauseef Ahmad Department of Civil Engineering UET PeshawarPakistan Electrical Engineering Department CECOS University of IT&Emerging Sciences PeshawarPakistan

In this paper eight successive experimental blast tests with an increasing TNT equivalent charge weights ranging from 0.56 kg to 17.78 kg were conducted on unreinforced,ferrocemented overlay masonry and confined masonry *** pressure-time history caused by the blast was recorded by pressure sensors installed on the test *** resulting damage pattern was observed during each *** zones in the three systems of masonry were *** distances for different damage levels in the three masonry systems were experimentally *** results provide a basis for determining the response of each masonry system against blast ***,efficiency of ferrocemented overlay masonry and confined masonry was found established in mitigation against blast loads.

关键词： Peak over pressure Pressure sensor Composition-B TNT Scaled distance Stand-off distance Charge shape Confined Unreinforced Ferrocemented

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