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基于IMB模型的出院准备计划对炎症性肠病患者自我管理能力的影响

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护理实践与研究 2022年第3期19卷 398-402页

作者：梁蒙蒙王颖樊慧丽孟莹莹梁梦欣冯芹芹蒋明珠施雨徐州医科大学附属医院消化科江苏省徐州市221000

目的探讨基于信息-动机-行为技巧模型(IMB)的出院准备计划对炎症性肠病患者自我管理能力的影响。方法选取2019年8月—2020年8月炎症性肠病患者104例,按组间基本特征匹配原则分为观察组和对照组,每组52例。对照组行常规护理随访指导,观... 详细信息

目的探讨基于信息-动机-行为技巧模型(IMB)的出院准备计划对炎症性肠病患者自我管理能力的影响。方法选取2019年8月—2020年8月炎症性肠病患者104例,按组间基本特征匹配原则分为观察组和对照组,每组52例。对照组行常规护理随访指导,观察组实施基于IMB模型的出院准备计划,比较两组干预前后自我管理能力、用药依从性、预后情况及生活质量。结果干预后,观察组健康自我管理能力测定量表(AHSMSRS)评分、Morisky用药依从性量表评分及生活质量总评分较对照组明显提高,差异有统计学意义(P<0.05),而观察组干预后炎症性肠病患者症状群总评分明显低于对照组,差异有统计学意义(P<0.05)。结论基于IMB模型的出院准备计划能有效提高炎症性肠病患者出院后自我管理能力及用药依从性,从而减轻患者肠道症状,改善患者生活质量。

关键词： IMB模型出院准备计划炎症性肠病自我管理能力用药依从性肠道症状

BMP4 preserves the developmental potential of mESCs through Ube2s-and Chmp4b-mediated chromosomal stability safeguarding

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Protein & Cell 2022年第8期13卷 580-601页

作者： mingzhu Wang Kun Zhao Meng Liu Mengting Wang Zhibin Qiao Shanru Yi Yonghua jiang Xiaochen Kou Yanhong Zhao Jiqing Yin Tianming Li Hong Wang Cizhong jiang Shaorong Gao Jiayu Chen Clinical and Translation Research Center of Shanghai First Maternity&Infant Hospital Shanghai Key Laboratory of Signaling and Disease ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China Frontier Science Center for Stem Cell Research Tongji UniversityShanghai 200092China Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education Orthopaedic Department of Tongji HospitalTongi UniversityShanghai 200065China Institute for Regenerative Medicine Shanghai East HospitalSchool of Life Sciences and TechnologyTongji UniversityShanghai 200123China Guangxi Key Laboratory of Genomic and Personalized Medicine Nanning 530021China

Chemically defined medium is widely used for culturing mouse embryonic stem cells(mESCs),in which N2B27 works as a substitution for serum,and GSK3βand MEK inhibitors(2i)help to promote ground-state ***,recent studies suggested that MEKi might cause irreversible defects that compromise the developmental potential of ***,we demon-strated the deficient bone morphogenetic protein(BMP)signal in the chemically defined condition is one of the main causes for the impaired ***-cally,activating the BMP signal pathway by BMP4 could safeguard the chromosomal integrity and proliferation capacity of mESCs through regulating downstream tar-gets Ube2s and *** importantly,BMP4 pro-motes a distinct in vivo developmental potential and a long-term pluripotency ***,the pluripotent improvements driven by BMP4 are superior to those by attenuating MEK *** together,our study shows appropriate activation of BMP signal is essential for regulating functional pluripotency and reveals that BMP4 should be applied in the serum-free culture system.

关键词： BMP4 pluripotency chromosomal integrity developmental potential serum-lree

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我国中医药健康服务现状及提升路径研究

中国医院 2020年第3期24卷 25-27页

作者：贾二萍熊巨洋黎相麟彭莹莹蒋明珠沈晓任艳余海洋张亮华中科技大学同济医学院医药卫生管理学院武汉市430030 国家中医药管理局法监司北京市100026

目的:分析《中医药法》实施背景下中医药健康服务基本情况。方法:利用2017-2018年《中国卫生统计年鉴》《全国中医药统计摘编》及2018年5月底《中医药法》调研评估数据,对中医药医疗卫生资源与服务、中医预防和养生保健、政策和经费支... 详细信息

目的:分析《中医药法》实施背景下中医药健康服务基本情况。方法:利用2017-2018年《中国卫生统计年鉴》《全国中医药统计摘编》及2018年5月底《中医药法》调研评估数据,对中医药医疗卫生资源与服务、中医预防和养生保健、政策和经费支持等方面进行分析与研究。结果:2017年末,全国中医类医院、每千人口中医执业(助理)医师、每千人口公立中医类医院床位分别达4566所、0.38人、0.59张;截至2018年5月底,全国开办备案制中医诊所2894所;2017年全国中医类医疗卫生机构诊疗人次、出院人数分别达10.2亿人次、0.33亿人次;全国中医类医院提供中医治未病服务1983万人次;各省均已将中医药发展经费纳入本级财政预算。结论:《中医药法》实施后,中医药健康服务能力提升显著,但与居民多层次多样化健康需求仍有较大差距。建议优先补充和提高中医药健康服务人才队伍,围绕中医药健康服务特色,筑牢基层中医服务阵地,强化中医药法配套政策落实监管,建立中医药事业经费投入长效机制,完善中医药健康服务体系。

关键词：中医药法健康服务提升路径

改良非石蜡切片法荧光标记三维凝胶培养系统中形成的输卵管内膜干细胞团

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解剖学杂志 2021年第1期44卷 45-48,F0003页

作者：林芸秀江明珠闫培谢詹雄林淑萍叶浩峰汤璇魏玉珍杨信志福建医科大学基础医学院福州350122 福建医科大学临床医学院福州350122

目的 :研究改良非切片技术对细胞团中的细胞进行定性荧光标记的方法可行性及效果。方法 :将6~8周龄ICR雌鼠输卵管撕碎,用胶原蛋白酶消化20 min,将分散的输卵管混合细胞播种于三维凝胶培养系统中,培养6d后,用5-乙炔基-2'-脱氧尿苷(EdU)... 详细信息

目的 :研究改良非切片技术对细胞团中的细胞进行定性荧光标记的方法可行性及效果。方法 :将6~8周龄ICR雌鼠输卵管撕碎,用胶原蛋白酶消化20 min,将分散的输卵管混合细胞播种于三维凝胶培养系统中,培养6d后,用5-乙炔基-2'-脱氧尿苷(EdU)及膜蛋白上皮特异性黏附分子(EpCAM)标记凝胶培养系统中形成的细胞团。结果 :在非免疫荧光标记实验中,EdU插入到S期细胞的DNA中,并且被灵敏地检测出。免疫荧光标记实验中,用小鼠抗EpCAM标记细胞团。无论是免疫还是非免疫荧光标记,都可以得到很清晰的阳性结果。结论:改良后的非切片荧光标记法相较于传统的切片法更加方便、更加快捷。

关键词：三维凝胶培养系统细胞团荧光染色干细胞非石蜡切片

共溅射钨硅化合物真空快速热退火行为(英文)

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固体电子学研究与进展 1989年第4期 448-448页

作者：陈存礼曹明珠蒋宏伟徐伟文华文裕南京大学物理系华东工学院

The behavior of co-sputtering tungsten silicide after vacuum rapid annealing at 1000-1100℃ for 15s is investigated by Raman scattering, scanning electron microscope, X-ray diffraction, Auger electron spectrum and resistivity measurements. There are two Raman peaks at 331 and 450cm-1, and their intensities increase with the temperature rise of rapid thermal annealing. Scanning electron micrograph exhibits crystallization at 1000℃ and its enhancement with increase of annealing temperature. In addition to the (002), (101), (110), (103), (112) and (200) diffraction peaks of WSi2, there are some W5Si3 peaks in X-ray diffraction pattern. However, the composition of WSi2 is found in Auger electron spectrum at 1000℃, and the measurement results of sheet resistance also conclude WSi2 characteristics.

关键词：共溅射硅化合物快速热退火

CD4+T cells memorize obesity and promote weight regain

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Cellular & Molecular Immunology 2018年第6期15卷 630-639页

作者： jianghuan Zou Beibei Lai mingzhu Zheng Qin Chen Shujun jiang Anying Song Zan Huang Peiliang Shi Xin Tu Di Wang Linrong Lu Zhaoyu Lin Xiang Gao State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animal for Disease Study Model Animal Research CenterNanjing Biomedical Research InstituteNanjing University12 Xuefu RoadPukou DistrictNanjingJiangsu 210061China Institute of Immunology Program in Molecular and Cellular BiologyZhejiang University School of MedicineHangzhouZhejiang 310058China

Body weight regain often causes failure of obesity therapies while the underlying mechanism remains largely *** this study,we report that immune cells,especially CD4+T cells,mediate the‘memory’of previous obese *** a weight gain-loss-regain model,we found that C57BL/6J mice with an obesity history showed a much faster rate of body weight *** obesity memory could last for at least 2 months after previously obese mice were kept at the same body weight as non-obese ***,such obesity memory was abrogated by dexamethasone treatment,whereas immunodeficient Rag1−/−and H2A−/−mice failed to establish such ***1−/−mice repossessed the obesity memory when immune cells or CD4+T cells isolated from previously obese mice were ***,depletion of CD4+T cells led to obesity memory *** together,we conclude that CD4+T cells mediate obesity memory and promote weight regain.

关键词： CD4+T cell immunodeficiency metabolism obesity memory weight regain

Comprehensive transcriptional atlas of human adenomyosis deciphered by the integration of single-cell RNA-sequencing and spatial transcriptomics

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Protein & Cell 2024年第7期15卷 530-546页

作者： Tao Chen Yiliang Xu Xiaocui Xu Jianzhang Wang Zhiruo Qiu Yayuan Yu Xiaohong jiang Wanqi Shao Dandan Bai mingzhu Wang Shuyan Mei Tao Cheng Li Wu Shaorong Gao Xuan Che Department of Obstetrics and Gynecology Affiliated Women and Children Hospital of Jiaxing UniversityJiaxing 314000China Shanghai Key Laboratory of Maternal Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China Key Laboratory of Animal Bioengineering and Disease Prevention of Shandong Province College of Animal Science and TechnologyShandong Agricultural UniversityTaian 271018China Women’s Hospital School of MedicineZhejiang UniversityHangzhou 310013China Postgraduate training base Alliance of Wenzhou Medical University Wenzhou Medical UniversityWenzhou 325035China Jiaxing University Master Degree Cultivation Base Zhejiang Chinese Medical UniversityHangzhou 310053China Shanghai Key Laboratory of Maternal Fetal Medicine Shanghai Institute of Maternal-Fetal Medicine and Gynecologic OncologyClinical and Translational Research CenterShanghai First Maternity and Infant HospitalSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China

Adenomyosis is a poorly understood gynecological disorder lacking effective *** persists regarding“invagination”and“metaplasia”*** endometrial-myometrial junction(EMJ)connects the endometrium and myometrium and is important for diagnosing and classifying adenomyosis,but its in-depth study is just *** single-cell RNA sequencing and spatial profiling,we mapped transcriptional alterations across eutopic endometrium,lesions,and *** lesions,we identified unique epithelial(LGR5+)and invasive stromal(PKIB+)subpopulations,along with WFDC1+progenitor cells,supporting a complex interplay between“invagination”and“metaplasia”theories of ***,we observed endothelial cell heterogeneity and abnormal angiogenic signaling involving vascular endothelial growth factor and angiopoietin ***-cell communication differed markedly between ectopic and eutopic endometrium,with aberrant signaling in lesions involving pleiotrophin,TWEAK,and WNT *** study reveals unique stem cell-like and invasive cell subpopulations within adenomyosis lesions identified,dysfunctional signaling,and EMJ abnormalities critical to developing precise diagnostic and therapeutic strategies.

关键词： adenomyosis single-cell RNA sequencing spatial transcriptomics endometrial-myometrial junction progenitor cells

Response of the Ginseng C_(2)H_(2)-Type Zinc Finger Protein Family PgZFPs Gene to Methyl Jasmonate Regulation

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Phyton-International Journal of Experimental Botany 2024年第11期93卷 3055-3071页

作者： Yue jiang Lingyu Liu Kangyu Wang mingzhu Zhao Ping Chen Jun Lei Yanfang Wang Meiping Zhang Yi Wang Guang Chen College of Life Science Jilin Agricultural UniversityChangchun130118China Research Center for Ginseng Genetic Resources Development and Utilization Jilin ProvinceJilin Agricultural UniversityChangchun130118China College of Chinese Medicinal Materials Jilin Agricultural UniversityChangchun130118China

The main active components of ginseng are ginsenosides,which play signiﬁcant roles in treating cardiovascular diseases,cancer,and providing antioxidant *** are primarily synthesized through the mevalo-nate pathway and the methylerythritol phosphate *** key enzyme genes involved in this biosynthetic process have been cloned and validated,yet the regulatory functions of transcription factors remain *** C_(2)H_(2)-type zincﬁnger protein family,one of the largest families of transcription factors,is crucial in plant growth and development,response to biotic and abiotic stresses,and regulation of secondary *** study,based on the ginseng transcriptome database from Jilin,conducted a correlation analysis between the expression levels of PgZFPs genes in the Jilin ginseng C_(2)H_(2)-type zincﬁnger protein family and ginsenoside content,a gen-ome-wide association study of PgZFPs,and co-expression analysis of PgZFPs with validated key enzyme ***,ﬁve candidate genes involved in ginsenoside biosynthesis were identiﬁ*** involvement of PgZFP27 and PgZFP-59-02 genes from the PgZFPs family in the biosynthesis of ginsenosides was validated through in vitro methyl jasmonate(MeJA)induction *** result provides new genetic resources for the biosynthesis of ginsenosides.

关键词： Ginsenosides C_(2)H_(2)-type zincﬁnger protein family MeJA induction functional study SIT SITIENS FLC FLACCA MVA Mevalonate MEP 2-C-methyl-D-erythritol 4-phosphate GWAS Genome-wide association studies HPLC High-performance liquid chromatography

Automatic microinjection of zebrafish larvae based on injection effect detection

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Automatic microinjection of zebrafish larvae based on inject...