Comprehensive transcriptional atlas of human adenomyosis deciphered by the integration of single-cell RNA-sequencing and spatial transcriptomics

作     者：Tao Chen Yiliang Xu Xiaocui Xu Jianzhang Wang Zhiruo Qiu Yayuan Yu Xiaohong Jiang Wanqi Shao Dandan Bai Mingzhu Wang Shuyan Mei Tao Cheng Li Wu Shaorong Gao Xuan Che Tao Chen;Yiliang Xu;Xiaocui Xu;Jianzhang Wang;Zhiruo Qiu;Yayuan Yu;Xiaohong Jiang;Wanqi Shao;Dandan Bai;Mingzhu Wang;Shuyan Mei;Tao Cheng;Li Wu;Shaorong Gao;Xuan Che

作者机构：Department of Obstetrics and GynecologyAffiliated Women and Children Hospital of Jiaxing UniversityJiaxing 314000China Shanghai Key Laboratory of Maternal Fetal MedicineClinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China Key Laboratory of Animal Bioengineering and Disease Prevention of Shandong ProvinceCollege of Animal Science and TechnologyShandong Agricultural UniversityTaian 271018China Women’s HospitalSchool of MedicineZhejiang UniversityHangzhou 310013China Postgraduate training base Alliance of Wenzhou Medical UniversityWenzhou Medical UniversityWenzhou 325035China Jiaxing University Master Degree Cultivation BaseZhejiang Chinese Medical UniversityHangzhou 310053China Shanghai Key Laboratory of Maternal Fetal MedicineShanghai Institute of Maternal-Fetal Medicine and Gynecologic OncologyClinical and Translational Research CenterShanghai First Maternity and Infant HospitalSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2024年第15卷第7期

页      面：530-546页

核心收录：

学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

基　　金：National Natural Science Foundation of China(Nos.32270840,31721003 and 32270908) Shanghai Key Laboratory of Maternal Fetal Medicine(No.mfmkf202201) Natural Science Foundation of Zhejiang Province(No.LTGY24H040002) Jiaxing Municipal Public Welfare Research Project(No.2021AY30004) 

主　　题：adenomyosis single-cell RNA sequencing spatial transcriptomics endometrial-myometrial junction progenitor cells 

摘      要：Adenomyosis is a poorly understood gynecological disorder lacking effective *** persists regarding“invaginationand“metaplasia*** endometrial-myometrial junction(EMJ)connects the endometrium and myometrium and is important for diagnosing and classifying adenomyosis,but its in-depth study is just *** single-cell RNA sequencing and spatial profiling,we mapped transcriptional alterations across eutopic endometrium,lesions,and *** lesions,we identified unique epithelial(LGR5+)and invasive stromal(PKIB+)subpopulations,along with WFDC1+progenitor cells,supporting a complex interplay between“invaginationand“metaplasiatheories of ***,we observed endothelial cell heterogeneity and abnormal angiogenic signaling involving vascular endothelial growth factor and angiopoietin ***-cell communication differed markedly between ectopic and eutopic endometrium,with aberrant signaling in lesions involving pleiotrophin,TWEAK,and WNT *** study reveals unique stem cell-like and invasive cell subpopulations within adenomyosis lesions identified,dysfunctional signaling,and EMJ abnormalities critical to developing precise diagnostic and therapeutic strategies.