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Research Progress of Deep Eutectic Solvents in Extraction of Plant Flavonoids

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Agricultural Biotechnology 2024年第3期13卷 34-40,44页

作者： Zhen LIN shengmei xu Shaomei CHEN Cui wang Jian CHEN Fujian Vocational College of Bio-engineering Fuzhou 350002China Customs"Belt and Road"Information Sharing Application Innovation Laboratory Fujian Provincial Key Laboratory of Inspection and Quarantine Technology Research CenterFuzhou Customs Technology CenterFuzhou 350003China

As a new type of green solvents,deep eutectic solvents(DESs)have the advantages of strong extraction ability,designability,simple preparation,low price,recoverability and biodegradation,and show great application potential in the field of plant flavonoid *** this paper,the definition,classification and preparation methods of DESs were *** effects of DES composition,molar ratio of DES components,water content of DES systems,liquid-material ratio,extraction temperature,extraction time and extraction auxiliary techniques on the extraction yield of plant flavonoids were *** recycling methods of DESs were *** problems of DESs in the field of plant flavonoids extraction were pointed out,and further research direction and trend were analyzed and prospected.

关键词： Deep eutectic solvent Plant flavonoid Extraction Assistive technique Regeneration

外源钙离子对高温胁迫下草莓幼苗影响的转录组分析

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分子植物育种 2024年第11期22卷 3525-3532页

作者：张圣美裘波音徐谦黄宗安罗天宽温州科技职业学院农业与生物技术学院浙南作物育种重点实验室温州市特色作物生物技术创新重点实验室温州325006 温州市农业科学研究院温州325006 福建省农业科学院作物研究所福建省蔬菜遗传育种重点实验室福州350013

为探究添加外源钙离子后高温胁迫下草莓幼苗叶片的基因表达变化情况,本试验以‘红颜’草莓幼苗为材料,设置4个试验处理,分别为对照(昼/夜25℃/17℃16 h/8 h)每株喷施20 mL蒸馏水、对照每株喷施20mL的8mmol/L CaCl2溶液、高温(昼/夜42℃... 详细信息

为探究添加外源钙离子后高温胁迫下草莓幼苗叶片的基因表达变化情况,本试验以‘红颜’草莓幼苗为材料,设置4个试验处理,分别为对照(昼/夜25℃/17℃16 h/8 h)每株喷施20 mL蒸馏水、对照每株喷施20mL的8mmol/L CaCl2溶液、高温(昼/夜42℃/34℃16h/8h)每株喷施20mL蒸馏水、高温每株喷施20 mL的8 mmol/L CaCl2溶液,在高温胁迫第3天时取叶片进行转录组测序和生物信息学分析。结果表明:与高温喷施蒸馏水处理组相比,高温喷施外源钙离子处理组发生差异表达的基因共有2452个,其中1504个上调表达,948个下调表达;GO富集分析显示在细胞组成中,差异基因显著富集在细胞、细胞部分和细胞器;在分子功能中显著富集的条目是结合、催化活性、结构分子活性;显著富集的生物过程为代谢过程、细胞过程、刺激应答等;KEGG富集分析表明显著富集的代谢通路有植物与病原体的相互作用和MAPK植物信号传导途径-植物和植物激素信号转导等,进一步分析发现添加外源钙离子会使一些编码钙离子感受蛋白、WRKY转录因子、MYB转录因子、热激转录因子和热激转录蛋白的基因响应高温胁迫而发生差异表达,这为探究Ca2+信号在草莓幼苗应对高温胁迫机制中发挥的作用提供有价值的数据信息。

关键词：草莓幼苗 CaCl2 高温胁迫转录组

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牦牛全基因组单纯型微卫星分布特征分析

中国农业大学学报 2024年第3期29卷 134-142页

作者：徐宇辉马志杰郭卫兴莫延新陈生梅青海大学畜牧兽医科学院/农业农村部青藏高原畜禽遗传育种重点实验室/青海省高原家畜遗传资源保护与创新利用重点实验室西宁810016 青海省畜禽遗传资源保护利用中心西宁810016

为探究牦牛全基因组中重复序列的组成特征,本研究以染色体水平的牦牛全基因组(BosGru3.1)为研究对象,利用生物信息学方法对牦牛基因组中的单纯型SSRs序列进行了检测,并对其分布特征进行综合分析。结果表明:1)在牦牛全基因组(2.83 Gb)中... 详细信息

为探究牦牛全基因组中重复序列的组成特征,本研究以染色体水平的牦牛全基因组(BosGru3.1)为研究对象,利用生物信息学方法对牦牛基因组中的单纯型SSRs序列进行了检测,并对其分布特征进行综合分析。结果表明:1)在牦牛全基因组(2.83 Gb)中,共筛选出778 413个单纯型SSRs,总长度为14.42 Mb,占全基因组序列总长的0.51%,相对频率和相对密度分别为274.92 loci/Mb和5 094.43 bp/Mb。2)牦牛31条染色体中,1号染色体所含的SSRs数量最多(48 592个,6.24%),29号染色体所含的SSRs数量最少(11 758个,1.51%),染色体DNA序列长度与其所含SSRs数量呈显著正相关(P<0.001)。3)6种重复类型的单纯型SSRs在牦牛基因组中分布不均匀,其中单碱基重复类型的SSRs数量最多(320 592个,41.19%),其次是两碱基(197 824个,25.41%)、三碱基(130441个,16.76%)、五碱基(75 056个,9.64%)、四碱基(52 523个,6.75%)和六碱基(1 977个,0.25%)。4)各重复类型分别以A、AC、AT、AGC、AAC、AAAT、AAAC、AACTG、AGATC和AACCCT类别的SSRs分布较多,表现出明显的A(T)碱基组成优势。5)各重复类型的SSRs其重复拷贝数分别集中在12~25次(单碱基)、7~26次(两碱基)、5~13次(三碱基)、4~8次(四碱基)、4~7次(五碱基)和4~6次(六碱基)。综上,本研究揭示了牦牛全基因组中单纯型SSRs的分布特征,表明各重复类型的SSRs其频率、密度、优势碱基类别及重复拷贝数均存在差异,呈现出一定的规律性。本研究为深入了解牦牛基因组中重复序列特别是单纯型SSRs的组成和分布特征提供参考,也为后续发掘牦牛特异性SSRs标记、构建遗传图谱及开展标记与性状间的关联分析等研究奠定基础。

关键词：牦牛基因组单纯型微卫星丰度

Obacunone activates the Nrf2-dependent antioxidant responses

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Protein & Cell 2016年第9期7卷 684-688页

作者： xu, shengmei Chen, Weimin Xie, Qingfeng xu, Yang Guangzhou Univ Chinese Med Affiliated Hosp 2 Guangdong Prov Acad Chinese Med Sci Ctr Regenerat & Translat Med Guangzhou 510632 Guangdong Peoples R China Univ Calif San Diego Div Biol Sci 9500 Gilman Dr La Jolla CA 92093 USA

Dear Editor,The transcription factor nuclear factor erythroid 2-related factor 2 （Nrf2） plays a crucial role in human antioxidant defense response against environmental insults. Small molecular chemical activators of Nrf2 can confer protection against oxidative insults and inhibit the progression of diseases related to oxidative stress. Here, we identified oba- cunone as a novel activator of Nrf2 by decreasing Nrf2 ubiquitination and increasing its stability. In support of these findings, the systemic administration of obacunone strongly inhibits bleomycin-induced lung fibrosis in mice. Therefore,

关键词： Sulforaphane Tanshinone Nrf2 Activator Environmental Insult Nrf2 Pathway

Specialized Microglia Resolve Neuropathic Pain in the Spinal Cord

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Neuroscience Bulletin 2023年第1期39卷 173-175页

作者： Jing Yang Shulan Xie shengmei Zhu Zhen-Zhong xu Department of Anesthesiology and Department of Neurobiology of the First Affiliated Hospital Zhejiang University School of MedicineHangzhou310058China NHC and CAMS Key Laboratory of Medical Neurobiology MOE Frontier Science Center for Brain Research and Brain–Machine IntegrationSchool of Brain Science and Brain MedicineZhejiang UniversityHangzhou310058China

Neuropathic pain is a major health problem affecting up to 10%of the general population,resulting from a lesion or dysfunction of the somatosensory nervous system,including peripheral nerve injury(PNI)^([1]).However,neuropathic pain is difficult to treat because of its obscure *** types of neuropathic pain have been modelled in rodents to study its underlying mechanisms and identify novel therapeutic targets.

关键词： pain mechanisms Spinal

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牦牛基因组结构变异研究进展

中国草食动物科学 2024年第5期44卷 64-70页

作者：刘瑞林徐东辉李瑞哲陈生梅马志杰青海大学畜牧兽医科学院西宁810016 农业农村部青藏高原畜禽遗传育种重点实验室西宁810016 青海省高原家畜遗传资源保护与创新利用重点实验室西宁810016

基因组结构变异(Structural variation,SV)通常是指基因组内影响DNA序列长度或方向导致其发生变化的变异形式,包括缺失、插入、重复、易位和倒位。SV通过基因剂量效应和位置效应直接或间接影响基因的表达,进而导致物种发生表型变异。近1... 详细信息

基因组结构变异(Structural variation,SV)通常是指基因组内影响DNA序列长度或方向导致其发生变化的变异形式,包括缺失、插入、重复、易位和倒位。SV通过基因剂量效应和位置效应直接或间接影响基因的表达,进而导致物种发生表型变异。近10年来,随着全基因组重测序技术和高密度芯片技术的快速发展,牦牛基因组SV研究也取得了重要进展。本文就基因组SV类型、形成机制和检测方法进行阐释,并将近10年来牦牛基因组SV的研究现状进行了综述,以期为牦牛组学的深入研究、加快牦牛分子育种进程提供参考。

关键词：牦牛基因组结构变异

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Studies on the Interaction Between Vanillin and β-Amyloid Protein via Fluorescence Spectroscopy and Atomic Force Microscopy

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Chemical Research in Chinese Universities 2016年第2期32卷 172-177页

作者： SONG shengmei MA xuewen ZHOU Yehong xu Maotian SHUANG Shaomin DONG Chuan Institute of Environmental Science Shanxi University Taiyuan 030006 P. R. China Key Laboratory of Biomolecular Recognition and Sensing of Shangqiu Normal University Shangqiu 476000 P. R. China

β-Amyloid（Afl） plaques and intracellular neurofibrillary lesions in the brain are markers of Alzheimer＇s disease（AD）. The ability to safely decrease Aft concentrations is potentially important as a preventive strategy for AD. The interactions between vanillin and Aft polypeptide were investigated via fluorescence spectroscopy and atomic force microscopy（AFM）. The results of fluorescence and synchronous spectroscopies illustrate that the intrinsic fluorescence of tyrosine（Tyr） residues in Aβ1-42 aggregates can be quenched strongly upon the formation of vanil- lin-Aβ-42 complex. Thioflavine T（ThT）-induced fluorescence changes indicated that Aβ1--42 aggregates could be disaggregated by vanillin, and the AFM images of Aβ1-42 enunciated the depolymerization of Aβ1-42 aggregates by vanillin in a dose-dependent manner. Vanillin may be a potential pharmacological agent for the treatment of AD.

关键词： β-Amyloid fibril Vanillin Interaction Fluorescence spectroscopy Atomic force microscopy

上肢锻炼联合FMEA护理对PICC置管患者医院获得性静脉血栓栓塞症发生率及内皮功能的影响

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中华全科医学 2024年第4期22卷 700-703页

作者：林晓非田圆林圆圆王建群许圣美王红霞温州医科大学附属第一医院血液内科浙江温州325000

目的探讨上肢锻炼联合失效模式与效应分析(FMEA)护理应用于经外周静脉置入中心静脉导管(PICC)置管患者的效果,以及对患者内皮功能、医院获得性静脉血栓栓塞症发生率的影响。方法选取2022年6—12月温州医科大学附属第一医院PICC置管患者... 详细信息

目的探讨上肢锻炼联合失效模式与效应分析(FMEA)护理应用于经外周静脉置入中心静脉导管(PICC)置管患者的效果,以及对患者内皮功能、医院获得性静脉血栓栓塞症发生率的影响。方法选取2022年6—12月温州医科大学附属第一医院PICC置管患者102例,按随机数表法分为对照组(51例,常规护理)与观察组(51例,上肢锻炼联合FMEA护理)。比较2组护理前后内皮功能(EMCs、FMD)、失效模式RPN值、锻炼依从性、静脉血栓栓塞症情况以及并发症发生情况。结果相较于对照组,观察组患者及其评估、导管因素、置管、冲管和封管、健康教育、日常照护等失效模式RPN值均明显更低(P<0.05)。相较于护理前,2组护理后EMCs值升高,FMD值降低。但是,观察组护理后EMCs低于对照组[(1094.59±101.10)%vs.(1205.73±109.55)%,P<0.05],观察组FMD值高于对照组[(4.66±0.48)%vs.(3.73±0.48)%,P<0.05]。护理后,相较于对照组,观察组锻炼依从性整体更高(P<0.05),以及获得性静脉血栓栓塞症整体更轻(P<0.05)。并且,观察组并发症发生率更低(P<0.05)。结论上肢锻炼联合FMEA护理应用于PICC置管患者,可提高其锻炼依从性,改善患者内皮功能,降低医院获得性静脉血栓栓塞症发生率,有助于预防血栓的形成。

关键词： PICC置管失效模式与效应分析护理上肢锻炼医院获得性静脉血栓栓塞症内皮功能血液流变学

Chromatographic fingerprint analysis and quantitative evaluate the rhizomes of Alpinia officinarum Hance(lesser galangal)

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Journal of Chinese Pharmaceutical Sciences 2019年第10期28卷 728-738页

作者： Haoyan Jiao shengmei xu Chunlin Fan Qingwen Zhang Ying wang School of Pharmacy Guangdong Food and Drug Vocational CollegeGuangzhou 510520China Institute of Traditional Chinese Medicine&Natural Products College of PharmacyJinan UniversityGuangzhou 510000China JNU-HKUST Joint Laboratory for Neuroscience&Innovative Drug Research Jinan UniversityGuangzhou 510000China Institute of Chinese Medical Sciences University of MacaoMacao 999078China

The rhizome of Alpinia officinarum Hance is a well-known traditional Chinese medicine(TCM)and has been widely used for the remedy of gastrointestinal *** the present study,a simple and rapid HPLC-DAD was developed for the quality control of the rhizomes of *** chemical fingerprint was established using raw material of 15 different origins in *** analysis(SA)and hierarchical clusting analysis(HCA)were applied to select the qualitative *** components analysis(PCA)was conducted to select the quantitative markers of the rhizomes of *** samples from different *** constituents were confirmed by(+)electrospray ionization LC-MS.12 constituents were selected as common peaks and 10 of them were confirmed by(+)electrospray ionization *** bioactive constituents including DPHA,galangin flavanone,galangin,galangin 3-methylether,DPHB and DPHC were simultaneous determination by using the HPLC-DAD *** developed method was able to determine the bioactive components with excellent resolution,precision and *** results indicated that chromatographic fingerprint combination with multi-components determination method is suitable for quality assessment of the rhizomes of ***.

关键词： Alpinia officinarum Hance Simultaneous determination Chromatographic fingerprint Quality evaluation Traditional Chinese medicine