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Ion-ion reactions for charge reduction of biopolymer at atmospheric pressure ambient

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Chinese Chemical Letters 2007年第1期18卷 115-117页

作者： yue ming Zhou Jian Hua Ding Xie Zhang Huan Wen Chen Chemistry Department Jilin University Changchun 130023 China Applied Chemistry Department East China Institute of Technology Fuzhou 344000 China

Extractive electrospray ionization source （EESI） was adapted for ion-ion reaction, which was demonstrated by using a linear quadrupole ion trap mass spectrometer for the first ion-ion reaction of biopolymers in the ... 详细信息

关键词： EESI Ion-ion reaction Biopolymers Atmospheric pressure

Integrating morphological spatial pattern analysis and the minimal cumulative resistance model to optimize urban ecological networks: a case study in Shenzhen City, China

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Ecological Processes 2021年第1期10卷 862-876页

作者： Yang‑Yang Li Yu‑Zhe Zhang Zhi‑Yun Jiang Cheng‑Xuan Guo ming‑yue Zhao Zhi‑Guang Yang ming‑Yan Guo Bing‑yue Wu Quan‑Ling Chen School of Geography South China Normal UniversityNo.55West of Zhong‑shan AvenueTianhe DistrictGuangzhou 510631China. Institute of Environ‑ment and Sustainable Development in Agriculture Chinese Academy of Agri‑cultural SciencesBeijing 100081China. School of Natural Resources Faculty of Geographical ScienceBeijing Normal UniversityBeijing 100875China

Background:With the increasing fragmentation of landscape induced by rapid urbanization,the construction of ecological networks is of great signifcance to alleviate the degradation of urban habitats and protect natural envi‑***,there is considerable uncertainty when constructing ecological networks,especially the difer‑ent approaches to selecting ecological *** used the southern Chinese city of Shenzhen as a study area to construct and optimize ecological networks using a coupling *** source areas were extracted using morphological spatial pattern analysis(MSPA)and the landscape index *** networks were con‑structed using the minimal cumulative resistance(MCR)model and the gravity *** stones and ecological fault points were added in corridors to optimize the ecological ***:Ten core areas with maximum importance patch values were extracted by the landscape index method as ecological source areas according to MSPA,after which corridors between ecological sources were constructed based on the MCR *** constructed ecological networks were optimized using 35 stepping stones and 17 ecologi‑cal fault *** optimized ecological networks included 11 important corridors,34 general corridors,and seven potential *** results of corridor landscape-type analysis showed that a suitable ecological corridor is 60 to 200 m ***:Overall,our results imply that ecological source areas can be identifed virtually,and that ecological networks can be signifcantly optimized by combining MSPA and MCR *** results provide a methodologi‑cal reference for constructing ecological networks,and they will be useful for urban planning and biodiversity protec‑tion in Shenzhen and other similar regions around the world.

关键词： MSPA analysis MCR model Ecological corridor Ecological resistance surface Shenzhen City

Massive GGAAs in genomic repetitive sequences serve as a nuclear reservoir of NF-κB

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Journal of Genetics and Genomics 2018年第4期45卷 193-203页

作者： Jian Wu Qiao Wang Wei Dai Wei Wang ming yue Jinke Wang State Key Laboratory of Bioelectronics Southeast University Department of Infectious Diseases The First Affiliated Hospital of Nanjing Medical University

Nuclear factor kB（NF-kB） is a DNA-binding transcription factor. Characterizing its genomic binding sites is crucial for understanding its gene regulatory function and mechanism in cells. This study characterized the binding sites of NF-kB ReIA/p65 in the tumor neurosis factor-a（TNFa） stimulated HeLa cells by a precise chromatin immunoprecipitation-sequencing（ChIP-seq）. The results revealed that NF-kB binds nontraditional motifs（nt-motifs） containing conserved GGAA quadruplet. Moreover, nt-motifs mainly distribute in the peaks nearby centromeres that contain a larger number of repetitive elements such as satellite, simple repeats and short interspersed nuclear elements（SINEs）. This intracellular binding pattern was then confirmed by the in vitro detection, indicating that NF-kB dimers can bind the nontraditional kB（nt-KB） sites with low affinity. However, this binding hardly activates *** study thus deduced that NF-kB binding nt-motifs may realize functions other than gene regulation as NF-kB binding traditional motifs（t-motifs）. To testify the deduction, many ChIP-seq data of other cell lines were then analyzed. The results indicate that NF-kB binding nt-motifs is also widely present in other cells. The ChIP-seq data analysis also revealed that nt-motifs more widely distribute in the peaks with low-fold enrichment. Importantly, it was also found that NF-kB binding nt-motifs is mainly present in the resting cells, whereas NF-kB binding t-motifs is mainly present in the stimulated cells. Aston？ishingly, no known function was enriched by the gene annotation of nt-motif peaks. Based on these results, this study proposed that the nt-KB sites that extensively distribute in larger numbers of repeat elements function as a nuclear reservoir of NF-kB. The nuclear NF-kB proteins stored at nt-KB sites in the resting cells may be recruited to the t-KB sites for regulating its target genes upon stimulation.

关键词： NF-κB Binding region GGAA Storage

Micromagnetism simulation on effects of soft phase size on Nd2Fe14B/α-Fe nanocomposite magnet with soft phase imbedded in hard phase

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Chinese Physics B 2018年第8期27卷 529-534页

作者： Yu-Qing Li ming yue Yi Pen Hong-Guo Zhang College of Materials Science and Engineering Beijing University of TechnologyBeijing 100124China

In this study, micromagnetism simulation by using timte dltterence method is cameo out on the Ncl21-el415/a-Fe nanocomposite magnet with soft phase imbedded in hard phase. The effects of soft magnetic phase size （S） on the magnetic properties and magnetic reversal modes are systematically analyzed. As S increases from 1 nm to 48 nm, the remanence （Jr） increases, while the coercivity （Hci） decreases, leading to the result that the magnetic energy prod- uct [（BH）max] first increases slowly, and then decreases rapidly, peaking at S = 24 nm with the （BH）max of 72.9 MGOe （1 MGOe = 7.95775 kJ.m-3）. Besides, with the increase of S, the coercivity mechanism of the nanocomposite magnet changes from nucleation to pinning. Furthermore, by observing the magnetic moment evolution in demagnetization pro- cess, the magnetic reversal of the soft phase in the nanocomposite magnet can be divided into three modes with the increase of S： coherent rotation （S 〈 3 nm）, quasi-coherent rotation （3 nm≤S 〈 36 nm）, and the vortex-like rotation （S ≥36 nm）.

关键词： micromagnetism simulation magnetization dynamics, Nd2Fe14B/a-Fe nanocomposite magnets

Efficient aero-structural design optimization: Coupling based on reverse iteration of structural model

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Science China(Technological Sciences) 2015年第2期58卷 307-315页

作者： ZUO Ying Tao GAO Zheng Hong CHEN Gang WANG Xiao Peng LI yue ming National Key Laboratory of Aerodynamic Design and Research Northwestern Polytechnical University State Key Laboratory for Strength and Vibration of Mechanical Structures Xi'an Jiaotong University Shanghai Electro-Mechanical Engineering Institute

Traditional coupled multi-disciplinary design optimization based on computational fluid dynamics/computational structure dynamics(CFD/CSD)aims to optimize the jig shape of aircraft,and general multi-disciplinary design optimization methodology is *** special consideration is given to the aircraft itself during the *** main drawback of these methodologies is the huge expanse and the low *** solve this problem,we put forward to optimize the cruise shape directly based on the fact that the cruise shape can be transformed into jig shape,and a methodology named reverse iteration of structural model(RISM)is proposed to get the aero-structural performance of cruise *** main advantage of RISM is that the efficiency can be improved by at least four times compared with loosely-coupled aeroelastic analysis and it maintains almost the same fidelity of loosely-coupled aeroelastic *** optimization framework based on RISM is *** aerodynamic and structural performances can be optimized simultaneously in this framework,so it may lead to the true optimal *** aerodynamic performance was predicted by N-S solver in this *** shows that RISM predicts the aerodynamic and structural performances very well.A wing-body configuration was optimized by the proposed optimization *** drag and weight of the aircraft are decreased after optimization,which shows the effectiveness of the proposed framework.

关键词： aero-structural optimization reverse iteration cruise shape CFD/CSD true optimum semi-coupled

Heterologous expression and characterization of man gene from Bacillus Subtilis in Pichia Pastoris

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Frontiers in Biology 2008年第1期3卷 26-31页

作者： Yu QIAO Xiaobing CHEN Hongbiao DING ming yue Feed Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China

β-Mannanase catalyzes endo-wise hydrolysis of the backbone of mannan and heteromannan,which are abun-dant in the cell wall structure of ungerminated leguminous *** mature β-mannanase originated from Bacillus subtilis was expressed in Pichia pastoris,a methylotrophic yeast,using the leader peptide sequence of Saccharomyces cerevisiae α-*** cultivation of β-mannanase express-ing Pichia pastoris yields up to 1.8 g/L *** the super-natant the activity of the 40 kDa—total mannanase attained a level of 1102.0 IU/*** properties of the β-mannanase were *** pH and temperature for the recombinant enzyme were 5.5 and 50℃ *** enzyme was stable at pH 5.0-10.0 and maintained over 30%original activity after incubating at 70℃ for 30 min.

关键词： β-mannanase Pichiapastoris expression

Effect of hydroxyapatite nanoparticles on the growth and p53/c-Myc protein expression of implanted hepatic VX_2 tumor in rabbits by intravenous injection

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World Journal of Gastroenterology 2007年第20期13卷 2798-2802页

作者： Jun Hu Zhi-Su Liu Sheng-Li Tang yue-ming He Department of General Surgery Zhongnan Hospital Wuhan University Wuhan 430071

AIM： To evaluate the effect of hydroxyapatite nano- particles （Nano HAP） by intravenous injection on the inhibition of implanted hepatic VX2 tumor growth in rabbits and cell p53/c-Myc protein expression. METHODS： 60 hepatic VX2 tumor-bearing rabbits was randomly divided into five groups. Nano HAP collosol 20 mglkg, 40 mg/kg, 5-FU solutions 20 mg/mL, mixed liquor of 5-FU solution 20 mg/mL and Nano HAP collosol 20 mg/kg were infused by vein, normal saline conducted as the control. The general state, weight, liver function and gross tumor volume were detected dynamically. The expression of p53 and c-Myc gene protein in tumor tissue was detected by immunohistochemistry methods. RESULTS： The growth of implanted hepatic VX2 tumors was significantly inhibited in all therapy groups, 3 wk after the injection, the tumor control rates in Nano HAP collosol groups were 25.5% and 32.5% respectively, and the gross tumor volumes were obviously less than that of control group. （24.81 ± 5.17 and 22.73 ± 4.23 vs 33.32 ± 5.26, P 〈 0.05）. The tumor control rate of 5-FU group was 43.7% （18.74 4± 4.40 vs 33.32 ± 5.26, P 〈 0.05）, but the general state of the animals after injection aggravated; and the adverse reaction in the drug combination group obviously decreased. Due to the effect of Nano HAP, the positive expression of tumor associated the mutated p53 and c-Myc in tumor tissue was decreased obviously compared with the control group. CONCLUSION： Nano HAP has evident inhibitory action on rabbit implanted hepatic VX2 tumor in vivo, which may be the result of decreasing the expression of the mutated p53 and c-myc, and drug combination can obviously decrease the adverse reaction of 5-FU.

关键词： Hydroxyapatite nanoparticles Rabbit VX2 Liver carcinoma p53 c-myc

A new selective fluorescent probe for lead ions

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Chinese Chemical Letters 2009年第3期20卷 326-329页

作者： Mao Zhong Tian Feng Feng Shuang ming Meng yue Hua Yuan School of Chemistry and Chemical Engineering Shanxi Datong University Datong 037009 China

A new fluorescent probe （BPbl） for Pb^2＋ has been synthesized, where diethanolamine （receptor） is linked with 4,4-difluoro-4- bora-3a, 4a-diaza-s-indacene （BODIPY）（fluorophore） via a methylene group （spacer）. The absorption （496 nm） and emission （505 nm） wavelengths are in visible range. The fluorescence quantum yields of the lead-free and lead-bound states of BPb1 in acetonitrile are 0.013 and 0.693, respectively. The large chelation enhanced fluorescence effect （CHEF） with Pb^2＋ can be explained by the blocking of the photoinduced electron transfer （PET） process.

关键词： Pb^2+ Selectivity Photo-induced electron transfer Fluorescence

Dual-metal zeolite imidazolate framework for efficient lithium storage boosted by synergistic effects and self-assembly 2D nanosheets

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Chinese Chemical Letters 2022年第6期33卷 3291-3295页

作者： ming yue Yajing Fu Canping Zhang Junxiao Fu Shiquan Wang Jianwen Liu College of Chemistry and Chemical Engineering&Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry&Ministry of Educational Key Laboratory for the Synthesis and Application of Organic Functional Molecules Hubei UniversityWuhan 430062China Jiangsu Pylon Battery Co. Ltd.Yangzhou 211400China

Metal-organic framework materials(MOFs),such as zeolitic imidazolate framework(ZIF),have been widely used in energy storage due to their advantages such as high structural stability,large specific surface,more active sites and skeleton ***,a novel two-dimensional(2D)Co Cu-ZIF was synthesized by a facile solvothermal *** as-prepared Co Cu-ZIF nanosheets exhibit an ultrahigh reversible capacity of 2287.4 m Ah/g and remains at 1172.1 m Ah/g after 300 cycles at a current density of 100 m A/g,far better than that of the single Co-ZIF and ***,the specific discharge capacity of Co Cu-ZIF nanosheets can maintain at about 590 m Ah/g after 1000 cycles at the current density of 2 A/*** to the synergistic effect of two metals,function of nitrogen in the molecular and selfassembly 2D nanosheets,our research can provide strong support for the practical application of Co Cu-ZIF materials in lithium ion batteries.

关键词： Lithium ion batteries Metal-organic frameworks Co Cu-ZIF nanosheets Synergistic effect Self-assembly