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Potassium bisperoxo(1,10-phenanthroline) oxovanadate suppresses proliferation of hippocampal neuronal cell lines by increasing DNA methyltransferases

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Neural Regeneration Research 2019年第5期14卷 826-833页

作者： Xiao-Li Tian Shu-Yuan Jiang Xiao-Lu Zhang jie Yang Jun-He Cui Xiao-lei Liu Ke-Rui Gong Shao-Chun Yan Chun-Yang Zhang Guo Shao Biomedicine Research Center Basic Medical College and Baotou Medical College of Neuroscience Institute Baotou Medical College Inner Mongolia Key Laboratory of Hypoxic Translational Medicine Baotou Medical College Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine Xuanwu Hospital Capital Medical University Department of Oral and Maxillofacial Surgery University of California San Francsico Department of Neurosurgery the First Affiliated Hospital of Baotou Medical College

Bisperoxo(1,10-phenanthroline) oxovanadate(BpV) can reportedly block the cell cycle. The present study examined whether BpV alters gene expression by affecting DNA methyltransferases(DNMTs), which would impact the cell cycle. Immortalized mouse hippocampal neuronal precursor cells(HT_(22)) were treated with 0.3 or 3 μM BpV. Proliferation, morphology, and viability of HT_(22) cells were detected with an IncuCyte real-time video imaging system or inverted microscope and 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium, respectively. mRNA and protein expression of DNMTs and p21 in HT_(22) cells was detected by real-time polymerase chain reaction and immunoblotting, respectively. In addition, DNMT activity was measured with an enzyme-linked immunosorbent assay. Effects of BpV on the cell cycle were analyzed using flow cytometry. Results demonstrated that treatment with 0.3 μM BpV did not affect cell proliferation, morphology, or viability; however, treatment with 3 μM BpV decreased cell viability, increased expression of both DNMT3B mRNA and protein, and inhibited the proliferation of HT_(22) cells; and 3 μM BpV also blocked the cell cycle and increased expression of the regulatory factor p21 by increasing DNMT expression in mouse hippocampal neurons.

关键词： nerve regeneration hippocampal neurons potassium bisperoxo(1,10-phenanthroline)oxovanadate DNA methyltransferase p21 HT22 cell cell cycle immunoblotting DNA methylation neural regeneration

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Biomaterials and regulatory science

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Journal of Materials Science & Technology 2022年第33期128卷 221-227页

作者： Xu Song Zhonglan Tang Wenbo Liu Kuan Chen jie Liang Bo Yuan Hai Lin Xiangdong Zhu Yujiang Fan Xinli Shi Peng Zhao lei Yang Kai Zhang Antonios G.Mikos Xingdong Zhang Institute of Regulatory Science for Medical Devices Sichuan UniversityChengdu 610064China National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China School of Biomedical Engineering Sichuan UniversityChengdu 610064China Center for Medical Device Evaluation National Medical Products AdministrationBeijing 100081China Sichuan Testing Center for Biomaterials and Medical Devices Chengdu 610064China Center for Health Sciences and Engineering School of Health Sciences and Biomedical EngineeringHebei University of TechnologyTianjin 300130China Departments of Bioengineering Chemical and Biomolecular EngineeringRice UniversityHoustonTX 77251USA

The fast development of both biomaterials and regulatory science calls for a convergence,which is addressed in this article via their link through medical products of biomaterials and related safety and efficacy *** updated definition of biomaterials,and concepts of biomaterials-related medical products and so-called medical-grade and implantable materials are firstly *** a brief overview of the concept and history of regulatory science and its assessment of safety and efficacy of medical products,as well as the currently ongoing biomaterials-related regulatory science programs are ***,the opportunities provided by regulatory science for biomaterials as well as challenges on how to develop a biomaterials-based regulatory science system are *** the first article in the field to elucidate the relationship between biomaterials and regulatory science,key take-home messages include(1)biomaterials alone are not medical products;(2)regulatory authorities approve/clear final medical products,not biomaterials;(3)there is no definition/regulation on the so-called medical-grade or implantable materials;and(4)safety and efficacy refer to final medical products,not biomaterials alone.

关键词： Biomaterials Medical products Regulatory science Safety Efficacy

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Minimally invasive maxillary sinus augmentation with simultaneous implantation on an elderly patient:A case report

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World Journal of Clinical Cases 2020年第24期8卷 6408-6417页

作者： Shi-Hui Yang Wan-Qi Yu Jing-jie Zhang Zhe Zhou Jun-Yan Wang Rui-Ning Shi Yan-Min Zhou Jing-Hui Zhao Fan-Rong meng Department of Dental Implantology School and Hospital of StomatologyJilin UniversityChangchun 130021Jilin ProvinceChina Department of Stomatology Aviation General HospitalBeijing 100000China

BACKGROUND In this case study,a minimally invasive transalveolar approach using platelet-rich fibrin and bone substitute with simultaneous implantation was carried out in an elderly *** analyzed the cone-beam computed tomography(CBCT)findings to evaluate bone *** SUMMARY A 65-year-old female with no contraindications for dental implants and loss of maxillary bilateral molars is *** by CBCT showed the available vertical bone height in the bilateral posterior maxilla was 0.5-6.8 mm in the left and 2.8-6.5 mm in the *** patient underwent a transalveolar approach using platelet-rich fibrin and bone substitute with simulataneous placement of an implant 10 mm in *** months post-surgery,the implant showed excellent osseointegration with the bone ***,full-ceramic crowns were ***-up at 2 years demonstrated satisfactory *** Platelet-rich fibrin and bone substitute can be used to augment the maxillary sinus with a vertical bone height less than 4 mm.

关键词： Maxillary sinus augmentation Bone substitute Bone regeneration Plateletrich fibrin Internal sinus floor elevation Case report

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HPLC-RID法测定不同厂家益母草药材中盐酸水苏碱含量

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湖南农业科学 2024年第2期 81-84页

作者：王秋萍杨邦祝宋善敏甘梦兰李满香何珺雷阳贵州农业职业学院贵州清镇551400 贵州大学贵州省生化工程中心贵州贵阳550025

建立了测定益母草中盐酸水苏碱含量的HPLC-RID方法,并测定分析了14家药厂的益母草中盐酸水苏碱的含量。结果表明:以Mercury益母草专用柱(250 mm×4.6 mm,5μm)为色谱柱,以70%乙腈溶液为流动相、流速1.0 mL/min、柱温45℃的条件下采用示... 详细信息

建立了测定益母草中盐酸水苏碱含量的HPLC-RID方法,并测定分析了14家药厂的益母草中盐酸水苏碱的含量。结果表明:以Mercury益母草专用柱(250 mm×4.6 mm,5μm)为色谱柱,以70%乙腈溶液为流动相、流速1.0 mL/min、柱温45℃的条件下采用示差检测器进行检测,盐酸水苏碱在0.100~0.523 mg/mL浓度范围内呈良好的线性关系;回归方程为y=91519 x+1342.2(R^(2)=0.9916),平均回收率100.07%,RSD为0.71%,且该方法具有重复性好、操作简便、快速、稳定、易于普及等优点;各厂家的益母草药材中盐酸水苏碱含量差异较大,14家厂家中仅有1367和8656这2家的益母草药材符合药典标准;盐酸水苏碱含量与益母草药材性状具有关联性,市民、药企和中药商等在购买益母草药材时,建议选择性状为叶多、青绿、茎细的益母草药材。

关键词：益母草盐酸水苏碱 HPLC-RID 不同厂家药材性状

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基于网络药理学和实验验证四君子汤治疗阿尔茨海默病的作用机制

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滨州医学院学报 2024年第2期47卷 142-146页

作者：王彦君张清马辉许勇孟渝涵靳若艺李晓莉叶蕾滨州医学院山东烟台264003

目的利用网络药理学和实验验证四君子汤治疗阿尔茨海默病(AD)的潜在作用机制。方法通过中药系统药理学数据库与分析平台(TCMSP)检索四君子汤的有效成分及相关靶点,采用DisGeNET和GeneCards数据库搜集AD靶点。采用String数据库建立其共... 详细信息

目的利用网络药理学和实验验证四君子汤治疗阿尔茨海默病(AD)的潜在作用机制。方法通过中药系统药理学数据库与分析平台(TCMSP)检索四君子汤的有效成分及相关靶点,采用DisGeNET和GeneCards数据库搜集AD靶点。采用String数据库建立其共有靶点蛋白互作(PPI)网络,并运用Cytoscape软件绘制“药物-活性成分-作用靶点-疾病”网络。采用DAVID对关键靶点进行基因本体(GO)和基因组百科全书(KEGG)富集分析。取24只3月龄SPF级APP/PS1双转基因雄性小鼠随机分为模型组和低、高剂量四君子汤实验组,另取8只C57BL/6小鼠作为阴性对照组。低、高剂量实验组连续灌胃四个月。采用Morris水迷宫实验检测小鼠记忆能力和空间探索能力,采用相关试剂盒检测超氧化物歧化酶(SOD)、乙酰胆碱酯酶(AChE)、丙二醛(MDA)等氧化应激指标水平,电镜检测脑组织病理学变化。结果最终筛选出四君子汤128个有效成分,并得到与AD共有靶点49个,其中以AKT1、Caspase-3(Casp3)、TNF等为核心靶点,与PI3K-Akt信号通路密切相关。动物实验证实,动物实验可改善小鼠认知功能,病理结果显示四君子汤能减轻其中线粒体损伤,生化指标显示四君子汤可提高小鼠脑内AChE水平,降低SOD、MDA水平。结论四君子汤可以减轻APP/PS1引起的小鼠认知功能改变、脑内病理变化及生化指标的变化,为进一步阐释其物质基础和机制提供依据。

关键词：四君子汤阿尔兹海默症网络药理学氧化应激

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双能C T联合临床病理特征预测结直肠癌Ki-67表达的价值

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中国CT和MRI杂志 2024年第2期22卷 142-145页

作者：朱文玲李雨蒙岳思宇陈新蕾邱慎满李绍东徐州医科大学附属医院影像科江苏徐州221002 徐州医科大学医学影像学院江苏徐州221002

目的探讨双能量CT(DECT)定量参数联合临床病理特征预测结直肠癌(CRC)增殖抗原Ki-67阳性表达的价值。方法回顾性收集行腹盆腔DECT双期增强检查且经手术病理证实的123例结直肠腺癌患者术前影像学资料及临床病理资料。根据术后病理结果将Ki... 详细信息

目的探讨双能量CT(DECT)定量参数联合临床病理特征预测结直肠癌(CRC)增殖抗原Ki-67阳性表达的价值。方法回顾性收集行腹盆腔DECT双期增强检查且经手术病理证实的123例结直肠腺癌患者术前影像学资料及临床病理资料。根据术后病理结果将Ki-67分为高表达和低表达两组。利用Siemens工作站进行图像后处理,获得入组患者动脉期和静脉期的碘浓度(IC)、标准化碘浓度(NIC)及能谱曲线斜率(λHU)。采用χ^(2)检验比较两组间临床病理资料的差异。采用独立样本t检验/Mann-Whitney U检验比较两组间能谱参数的差异;采用受试者工作特征曲线(ROC)评估有统计学意义的参数的预测效能。结果结直肠癌Ki-67高表达组和低表达组之间血清CA199水平差异有统计学意义(χ^(2)=5.559,P<0.05)。Ki-67高表达组双期IC值、NIC值及能谱曲线斜率均高于Ki-67低表达组,差异具有统计学意义(P<0.05)。结论DECT定量参数IC、NIC、λHU及血清CA199可在一定程度上反映结直肠癌细胞增殖程度,预测结直肠癌Ki-67阳性表达的水平。

关键词：结直肠癌双能量CT 肿瘤标记物 Ki-67表达

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RHBDF1 promotes AP-1-activated endothelial-mesenchymal transition in tumor fibrotic stroma formation

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2307-2310页

作者： Shan Gao Li-Song Zhang lei Wang Nan-Nan Xiao Hui Long Yi-Lun Yin Yu-meng Yang Zhen Xi Lu-Yuan Li Zhi-Song Zhang State Key Laboratory of Medicinal Chemical Biology College of PharmacyTianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjinChina State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology National Pesticide Engineering Research Center(Tianjin)Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)College of ChemistryNankai UniversityTianjinChina

Dear Editor,There is a growing body of evidence that the human rhomboid family-1 gene(RHBDF1)plays an important role in the modulation of tumor inflammatory(Supplementary ***)and hypoxic microenvironment.

关键词： tumor transition hypoxic

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基于小波包分析的航空发动机轴承故障诊断

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推进技术 2009年第3期30卷 328-331,341页

作者：韩磊洪杰王冬北京航空航天大学能源与动力工程学院北京100083 空军驻京丰地区代表室北京100074

振动分析是进行滚动轴承状态监测与故障诊断的重要手段。当轴承某一元件表面出现局部损伤时,产生周期性的冲击脉冲力。因此,原来的平稳振动信号变成了非平稳振动信号。傅里叶变换在频域上是完全局部化的,但它不能提供任何时域的局部化特... 详细信息

振动分析是进行滚动轴承状态监测与故障诊断的重要手段。当轴承某一元件表面出现局部损伤时,产生周期性的冲击脉冲力。因此,原来的平稳振动信号变成了非平稳振动信号。傅里叶变换在频域上是完全局部化的,但它不能提供任何时域的局部化特征,而窗口傅立叶变换尽管在时域和频域均具有一定的局部化特征,但其局部化却是固定不变的。针对常规方法难以准确分析非平稳信号的局限性,提出了基于小波分析的滚动轴承故障诊断方法,通过滚动轴承外表面损伤的实验信号进行小波包频谱分析,验证了基于小波分析的滚动轴承故障诊断方法是可靠、准确的,可进一步应用于航空发动机主轴轴承的状态监测和故障诊断。

关键词：滚动轴承航空发动机主轴轴承+ 故障诊断小波包分解+ 特征提取+

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Vascular endothelial growth factor concentration in vitreous humor of patients with severe proliferative diabetic retinopathy after intravitreal injection of conbercept as an adjunctive therapy for vitrectomy

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Chinese Medical Journal 2020年第6期133卷 664-669页

作者： Bing Li meng-Da Li Jun-jie Ye Zhe Chen Zi-Jian Guo Yu Di Department of Ophthalmology Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China Ophthalmology Department Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102218China Laboratory Medicine Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China

Background:Proliferative diabetic retinopathy(PDR)is a progressive stage of diabetic retinopathy featured by the formation of neovascular and proliferative *** endothelial growth factor(VEGF)acts as a pivot factor in the development of *** study was to investigate the changes of intravitreal VEGF concentrations of severe PDR after intravitreal injection of conbercept(IVC)and its potential advantages to the following ***:This was a prospective,interventional,randomized controlled *** eyes(60 patients)with severe PDR and 20 eyes from 20 patients with rhegmatogenous retinal detachment complicated with proliferative vitreoretinopathy were enrolled in this *** eyes were randomly assigned to three groups by sortation randomization method with 20 eyes in each based on the interval of preoperative IVC(group A:7 days,group B:14 days,group C:non-IVC).Another 20 eyes without diabetes were enrolled as the non-diabetic control group(group D),receiving PPV *** specimens of all 80 patients were collected and evaluated *** intravitreal VEGF concentration of the four groups,and the total surgical time and the intraoperative bleeding rate of the PDR groups were ***:The mean intravitreal VEGF concentrations of groups A-D were 66.6±43.3,93.1±52.3,161.4±106.1 and 1.8±1.2 pg/mL,*** increased significantly in PDR patients(groups A,B and C)(P=0.002,<0.001,and<0.001,respectively).PDR patients with preoperative IVC(groups A and B)presented significantly lower VEGF concentrations(P<0.001 and 0.001),intraoperative bleeding rates(P=0.004)and total surgical time(P<0.001,P=0.003)compared with group *** statistical differences were presented between groups A and B on the three ***:Seven days and 14 days of preoperative IVC are equally efficient and safe for the vitrectomy of severe PDR patients through decreasing vitreous VEGF concentrations,intraoperative bleeding ra

关键词： Anti-vascular endothelial growth factor Diabetic retinopathy/proliferative diabetic retinopathy Vitreous humor Vascular endothelial growth factor

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流域初始水权分配研究进展

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长江流域资源与环境 2008年第5期17卷 734-739页

作者：孟祺尹云松孟令杰南京农业大学经济管理学院江苏南京210095

当前我国水资源存在短缺和使用效率低下等问题,其最核心的解决办法就是建立水权市场,而其中的关键在于界定初始水权。通过界定初始水权,在各流域行政区域之间合理地分配。简要介绍了流域初始水权分配的最新研究进展和动态,主要包括分配... 详细信息

当前我国水资源存在短缺和使用效率低下等问题,其最核心的解决办法就是建立水权市场,而其中的关键在于界定初始水权。通过界定初始水权,在各流域行政区域之间合理地分配。简要介绍了流域初始水权分配的最新研究进展和动态,主要包括分配原则、分配指标体系、分配方法和分配机制等方面。对这些研究进行了评述并且指出了存在的问题。由于水资源分配的特殊性,涉及到水量、水质和其它一些因素,单纯的分配水权难以获得成功。在此基础上,探讨了流域初始水权分配的未来研究方向,即在考虑到了水权、排污权和林权等集成的基础上,综合考虑初始水权分配的原则、指标体系和机制等,并且在分配方法上由单一目标模型向多目标模型发展,不确定性和模糊优化模型是未来流域水资源分配模型的发展方向。

关键词：初始水权集成分配方法

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