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Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation:A preliminary study

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Asian Pacific Journal of Tropical Biomedicine 2016年第7期6卷 568-573页

作者： md.Moklesur Rahman sarker Long Chiau Ming md.zaidul islam sarker M Shahabuddin Kabir Choudhuri Department of Immunochemistry Division of Pharmaceutical SciencesOkayama University Graduate School of MedicineDentistry and Pharmaceutical Sciences Department of Pharmacology Faculty of PharmacyLincoln University College Faculty of Pharmacy Brain Degeneration and Therapeutics GroupPharmaceutical and Life Sciences Co Re Universiti Teknologi MARA Department of Pharmaceutical Technology Faculty of PharmacyInternational Islamic University Malaysia Department of Pharmacy Faculty of Biological ScienceJahangirnagar University

Objective:To assess the immunopotentiality of Ayurvedic polyherbal preparations,"Saribadi" and "Anantamul Salsa".Methods: Freshly prepared BALB/c mice splenocytes were cultured with "Saribadi" or"Anantamul Salsa" treatment [doses of 0.25%, 0.50%, 0.75%, 1.00%, 1.50%, 2.00%,3.00% and 4.00%(v/v)] at 37C for 5 days. The immunoglobulin M(IgM) production and lymphocytes proliferation were determined by ELISA and MTT methods, *** contamination was assessed by treating the preparations with polymyxin ***: The doses of "Saribadi" [0.25%, 0.50%, 0.75% and 1.00%(v/v)] significantly increased IgM productions(0.966, 0.728, 0.695 and 0.615 mg/m L vs. control 0.265 mg/m L)and lymphocytes proliferation [absorbance 0.311, 0.394, 0.372 and 0.334 optical density(OD) vs. control 0.162 OD]. Similarly, the doses of "Anantamul Salsa" [0.50%, 0.75%,1.00% and 1.50%(v/v)] promoted IgM productions(0.933, 0.919, 0.917 and 0.892 mg/m L vs. control 0.502 mg/m L) and the doses of "Anantamul Salsa" [0.50%, 0.75%, 1.00%,1.50%, 2.00%, and 3.00%(v/v)] stimulated lymphocytes proliferation(absorbance 0.395,0.326, 0.440, 0.398, 0.452 and 0.355 OD vs. control 0.199 OD). The activity of "Saribadi"and "Anantamul Salsa" was not retarded by the treatment of preparations with polymyxin ***: Immunomodulatory activity of "Saribadi" and "Anantamul Salsa" was unveiled for the first time. "Saribadi" and "Anantamul Salsa" possess immunostimulating potential acting through the induction of lymphocyte proliferation and IgM *** preparations may be useful in strengthening immune responses. However, further cellular and in vivo studies are required.

关键词： "Saribadi" "Anantamul Salsa" Ayurvedic medicine Immunostimulant Lymphocytes Splenocytes Immunoglobulin M antibody Proliferation Differentiation

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Clinacanthus nutans:a review of the medicinal uses,pharmacology and phytochemistry

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 393-399页

作者： md.Ariful Alam Sahena Ferdosh Kashif Ghafoor md.Abdul Hakim Abdul Shukor Juraimi Alfi Khatib md.zaidul I.sarker Faculty of Pharmacy.International islamic University Malaysia(IIUM) Kuantan Campus Faculty of Science International Islamic University Malaysia(IIUM).Kuantan Campus Department of Food Science and Nutrition King Saud University Institute of Tropical Agriculture University Putra Malaysia Department of Crop Science University Putra Malaysia

Clinacanthus nutans Lindau is known as snake grass belonging to the Acanlhaceae *** plant has diverse and potential medicinal uses in traditional herbal medicine for treating skin rashes,insects and snake bites,lesions caused by herpes simplex virus,diabetes,and gout in *** and *** investigations documented the varied contents of bioaclive compounds from litis plant namely flavonoids,glycosides,*** and *** pharmacological experiment proved that various types of extracts and pure compounds from this species exhibited a broad range of biological properties such as anti-inflammatory,antiviral,antioxidant,and anti-diabetic *** lindings of toxicity study showed that extracts from this plant did not show any toxicity thus it can be used as strong therapeutic agents for specific diseased ***,further experiments on chemical components and their mode of action showing biological activities are required to elucidate the complete phytochemical profile and assess to confirm their suitability tor future *** review summarizes the medicinal uses,phytochemistry and pharmacology of this plant in order to explore its therapeutic potential and gaps necessitating for prospected research work.

关键词： Clinacanthus nutans Medicinal uses Phytochemicals Pharmacology Therapeutic potential

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