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Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence

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Light(Science & Applications) 2023年第3期12卷 415-424页

作者： Leanne maurice Alberto Bilenca Biomedical Engineering Department Ben-Gurion University of the Negev1 Ben Gurion BlvdBe’er-Sheva84105Israel Ilse Katz Institute for Nanoscale Science and Technology Ben-Gurion University of the Negev1 Ben Gurion BlvdBe’er-Sheva84105Israel

Single particle tracking in three dimensions is an indispensable tool for studying dynamic processes in various disciplines,including material sciences,physics,and biology,but often shows anisotropic three-dimensional spatial localization precision,which restricts the tracking precision,and/or a limited number of particles that can be tracked simultaneously over extended *** we developed an interferometric,three-dimensional fluorescence single particle tracking method based on conventional widefield excitation and temporal phase-shift interference of the emitted,high-aperture-angle,fluorescence wavefronts in a greatly simplified,free-running,triangle interferometer that enables tracking of multiple particles at the same time with<10-nm spatial localization precision in all three dimensions over extended volumes(~35×35×2μm^(3))at video rate(25 Hz).We applied our method to characterize the microenvironment of living cells and up to~40μm deep in soft materials.

关键词： materials particle dimensions

Molar Tooth Structure:a Contribution from the Mesoproterozoic Gaoyuzhuang Formation,Tianjin City,North China

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Acta Geologica Sinica(English Edition) 2011年第5期85卷 1084-1099页

作者： maurice E.TUCKER Department of Earth Sciences Durham University

Molar-tooth(MT) structure is an enigmatic sedimentary structure consisting of variously-shaped cracks and voids filled with a characteristically uniform,equant calcite *** is globally distributed but temporally restricted to rocks from Neoarchean to Neoproterozoic *** origin of MT structures has been debated for more than a century and the topic continues to be highly *** features of MT structure occurring in micritic limestones of the Mesoproterozoic Gaoyuzhuang Formation(ca.1500 Ma to ca.1400 Ma),Jixian section,Tianjin City,North China show that:1) there is a definite interface or lining,rich in organic material and pyrite,between the MT crack-filling calcite microspar and the micritic host rock,which is also rich in organic matter;2) the micritic host rocks are notable for the absence of stromatolites and microbial laminites;3) distinctive conglomeratic lag deposits made up of intraclasts of MT microspar result from storm reworking of the MT structures;4) the MT structure is associated with possible algal megafossils such as Chuaria;5) the MT microspar is made up of the larger calcite crystal and the MT crack is marked by the diversity of configurations;6) both the TOC content and the carbon-isotopic value(δ^(13)C_(PDB)) among the host rock, the MT microspar and the possible algae fossil are obviously *** the forming mechanism of the Gaoyuzhuang MT structure,these features can still indicate that:A) the MT microspar was formed by rapid precipitation and lithification;B) the MT microspar precipitated directly within the cracks; C) the decomposition of organic matter within the host micrite might be the chief mechanism producing gas bubbles;D) microscale gas-sediment interaction led to the generation of the MT cracks and the precipitation of microspar therein;E) the MT cracks might represent the track of migration and expansion of gas bubbles,and that the recrystallization of host micrites cannot be eliminated during forming proc

关键词： Molar-tooth structure Mesoproterozoic limestone Gaoyuzhuang Formation Tianjin North China

CONTINUITY PROPERTIES FOR BORN-JORDAN OPERATORS WITH SYMBOLS IN HÖRMANDER CLASSES AND MODULATION SPACES

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Acta Mathematica Scientia 2020年第6期40卷 1603-1626页

作者： maurice de GOSSON Joachim TOFT Faculty of Mathematics NuHAGUniversity of ViennaViennaAustria Department of Mathematics Linnæus UniversityVäxjöSweden

We show that the Weyl symbol of a Born-Jordan operator is in the same class as the Born-Jordan symbol,when Hörmander symbols and certain types of modulation spaces are used as symbol *** use these properties to carry ... 详细信息

关键词： quantization Schatten-von Neumann Feffermann-Phong's inequality

NH_3 Plasma Surface Treatments of Engineering Fluoropolymers: A Way to Enhance Adhesion of Ni or Cu Thin Films Deposited by Electroless Plating

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材料热处理学报 2004年第05B期25卷 1092-1097页

作者： maurice Romand Marlene Charbonnier Yves Goepfert Laboratoire de Sciences et Ingenierie des Surfaces and Laboratoire de Sciences et Ingenierie des Surfaces andUMR 5180 des Sciences Analytiques Universite Claude Bernard - Lyon 1 69622 Villeurbanne Cedex France

This paper describes the electroless Ni or Cu plating of some fiuoropolymer substrates through a tin-free activation process. Materials subjected to surface metallization are commercial Teflon() FEP, Nafion(), ACLAR() and LaRCTM-CP1 thin films which have recently gained a large scientific and technological interest due to their excellent thermal, chemical, mechanical and dielectric properties. The original approach implemented in the present work involves: (i)the grafting of nitrogen-containing functionalities on the polymer surfaces through plasma treatments in ammonia, (ii) the direct catalysis of the so-modified surfaces via their immersion in a simple acidic PdCl2 solution (i.e. without using a prior surface sensitization in an acidic SnCl2 solution), and finally (iii) the electroless metallization itself. However, prior to the immersion in the industrial plating baths, the chemical reduction of the Pd+2 species (species covalently tethered on the nitrogen-containing groups) to metallic palladium (PdO) is shown to be a key factor in catalyzing the electroless deposition initiation. This is made by immersion in an hypophosphite (H2PO2-) solution. Wettability measurements and X-ray photoelectron spectroscopy (XPS) experiments are used to characterize every surface modification step of the developed process. A cross-hatch tape test was used to asses the adhesion strength of the electroless films that is shown qualitatively good. In addition, a fragmentation test was developed in combination with electrical measurements. Its use allows to distinguish different adhesion levels at the metal/polymer interface and to evidence the influence of some processing parameters.

关键词：镍化学镀层铜化学镀层等离子表面处理表面催化 XPS 剥离试验

Direct Ni Electroless Metallization of Poly(etherimide) without Using Palladium as a Catalyst

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材料热处理学报 2004年第05B期25卷 1106-1111页

作者： Marlène Charbonnier maurice Romand Yves Goepfert Laboratoire de Sciences et Ingenierie des Surfaces Universite Claude Bernard - Lyon 1 69622 Villeurbanne Cedex FranceLaboratoire de Sciences et Ingenierie des Surfaces Universite Claude Bernard - Lyon 1 69622 Villeurbanne Cedex FranceUMR 5180 des Sciences Analytiques Universite Claude Bernard - Lyon 1 69622 Villeurbanne Cedex France

Nickel or copper electroless metallization of polymers needs the grafting of a catalyst (palladium in the Pd(0) oxidation state) on the substrate surface to be coated. Our previous works on this topic [1-5] have allowed to develop a simple, tin-free method to attach Pd(+2) species from a palladium chloride (PdCl2) solution on any insulating surface and subsequently to reduce them, in the wet way, into the Pd(0) oxidation state. This Pd(0) state of the catalyst allows an instantaneous initiation of the Ni or Cu deposit by immersion in a plating bath. As palladium is an expensive chemical, it appears interesting to replace it by Ni(0) or Cu(0) species which are catalysts of their own ion reduction. Concerning the direct Ni electroless plating, the polymer surface (PI) was made catalytic for the electroless process by deposition (spin- coating or dipping) of an ultra-thin film of an organic nickel salt in an alcoholic solution. The chemical reduction of this salt, checked by XPS, was performed by chemical or photochemical ways and by plasma. Under these conditions, the initiation, by autocatalysis, of the Ni film deposition in an industrial plating bath was immediate i.e. without any initiation time. The film obtained was homogeneous, dense, bright, well-adhering up to thicknesses reaching

关键词：镍非电金属喷镀等离子处理 XPS 聚酰亚胺

Predicting a single HIV drug resistance measure from three international interpretation gold standards

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Asian Pacific Journal of Tropical Medicine 2012年第7期5卷 566-572页

作者： Singh Yashik Mars maurice Department of Telehealth Nelson R Mandela School of MedicineUniversity of KwaZulu-Natal719 Umbilo RoadUmbiloDurbanSouth Africa

Objective:To investigate the possibility of combining the interpretation of three gold standard interpretation algorithms using weighted heuristics in order to produce a single resistancemeasure. Methods:The outputs of HIVdb,Rega,ANRS were combined to obtain a single resistance profile using the equally weighted voting algorithm,accuracy based weighing voting algorithm and the Bayesian based weighted voting algorithm ***:The Bayesian based voting combination increased the accuracy of the resistance profile prediction compared to phenotype,from 58%to 69%.The equal weighted voting algorithm and the accuracy based algorithm both increased the prediction accuracy to 60%.Conclusions:From the result obtained it is evident that combining the gold standard interpretation algorithms may increase the predictive ability of the individual interpretation algorithms.

关键词： Drug resistance Antiretroviral therapy Highly active HIV Artificial intelligence Expert systems

Two-parameter families of uniquely extendable Diophantine triples

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Science China Mathematics 2018年第3期61卷 421-438页

作者： Mihai Cipu Yasutsugu Fujita maurice Mignotte Simion Stoilow Institute of Mathematics of the Romanian Academy Research unit nr. 5 P.O. Box 1-764 RO-014700 Bucharest Romania Department of Mathematics College of Industrial Technology Nihon University 2-11-1 Shin-ei Narashino Chiba 276-0028 Japan Département de Mathematique Universite de Strasbourg Strasbourg 67084 France

Let A and K be positive integers and ε∈ {-2,-1,1,2}. The main contribution of the paper is a proof that each of the D(ε~2)-triples {K, A^2 K+2εA,(A +1)~2 K + 2ε(A+1)} has uniqui extension to a D(ε~2)-quadruple. This is used to slightly strengthen the conditions required for the existencc of a D(1)-quintuple whose smallest three elements form a regular triple.

关键词： Diophantine m-tuples Pell equations hypergeometric method linear forms in logarithms

Sigma-1 receptor: culprit and rescuer in motor neuron diseases

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Neural Regeneration Research 2021年第1期16卷 106-107页

作者： Jean-Charles Liévens Tangui maurice MMDN Univ MontpellierINSERMEPHEMontpellierFrance

Initially,the Sigma-1 receptor(S1R)was incorrectly categorized as one of the opioid *** it has since become clear that S1R is a non-opioid non-phencyclidine receptor with molecular chaperone activity.S1R is a 223 amino-acid long protein that shares no clear homology with any other known mammalian proteins,its closest homolog being the fungal ERG2 sterol *** its pharmacological counterpart,the Sigma-2 receptor that was only recently cloned,is genetically distinct.S1R is more likely considered as an atypical ligand-modulated chaperone protein.S1R is widely expressed in many organs including brain where its function has mainly been explored.

关键词： organs opioid Sigma

Drought stress triggers alterations of adaxial and abaxial stomatal development in basil leaves increasing water-use efficiency

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Horticulture Research 2023年第6期10卷 96-109页

作者： Elisa Driesen maurice De Proft Wouter Saeys Department of Biosystems KU LeuvenWillem De Croylaan 423001 LeuvenBelgium

The physiological control of stomatal opening by which plants adjust for water availability has been extensively ***,the impact of water availability on stomatal development has not received as much attention,especially for amphistomatic ***,the acclimation of stomatal development in basil(Ocimum basilicum L.)leaves was *** results show that leaves developed under water-deficit conditions possess higher stomatal densities and decreased stomatal length for both the adaxial and abaxial leaf *** the stomatal developmental reaction to water deficit was similar for the two leaf surfaces,it was proven that adaxial stomata are more sensitive to water stress than abaxial stomata,with more closed adaxial stomata under water-deficit ***,plants with leaves containing smaller stomata at higher densities possessed a higher water use *** findings highlight the importance of stomatal development as a tool for long-term acclimation to limit water loss,with minimal reduction in biomass *** highlights the central role that stomata play in both the short(opening)and long-term(development)reaction of plants to water availability,making them key tools for efficient resource use and anticipation of future environmental changes.

关键词： stress stomatal limit