Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence

作     者：Leanne Maurice Alberto Bilenca Leanne Maurice;Alberto Bilenca

作者机构：Biomedical Engineering DepartmentBen-Gurion University of the Negev1 Ben Gurion BlvdBe’er-Sheva84105Israel Ilse Katz Institute for Nanoscale Science and TechnologyBen-Gurion University of the Negev1 Ben Gurion BlvdBe’er-Sheva84105Israel 

出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)

年 卷 期：2023年第12卷第3期

页      面：415-424页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：The authors thank Prof.Angel Porgador and Dr.Orly Gershoni-Yahalom of Ben-Gurion University of the Negev for the valuable assistance in the preparation of the biological samples.A.B.acknowledges the support of the Israel Science Foundation(grant no.1599/12) 

主　　题：materials particle dimensions 

摘      要：Single particle tracking in three dimensions is an indispensable tool for studying dynamic processes in various disciplines,including material sciences,physics,and biology,but often shows anisotropic three-dimensional spatial localization precision,which restricts the tracking precision,and/or a limited number of particles that can be tracked simultaneously over extended *** we developed an interferometric,three-dimensional fluorescence single particle tracking method based on conventional widefield excitation and temporal phase-shift interference of the emitted,high-aperture-angle,fluorescence wavefronts in a greatly simplified,free-running,triangle interferometer that enables tracking of multiple particles at the same time with10-nm spatial localization precision in all three dimensions over extended volumes(~35×35×2μm^(3))at video rate(25 Hz).We applied our method to characterize the microenvironment of living cells and up to~40μm deep in soft materials.