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Update on the 2nd Bioactive Materials Editorial Board Meeting

Bioactive Materials 2017年第4期2卷 287-288页

作者： mark p.staiger University of Canterbury E512Civil/MechanicalPrivate Bag 4800Christchurch 8140New Zealand

Following on from the 1st Editorial Board Meeting of Bioactive Materials(held April 2017,Minneapolis USA),the 2nd Editorial Board Meeting was held on the 28th August 2017 during the 9th Symposium on Biodegradable Metals for Biomedical Applications(http://***).(see Figs.1 and 2)The Symposiumwas held in Bertinoro Italy(Emilie-Romagne region),80 km southeast of *** year the Symposium attracts^150 participants working in the field of biodegradable metallic biomaterials,mainly based on Mg,Fe and Zn,with^50 oral presentations and 90 posters delivered at the 9th Symposium by participants from 22 *** were organised into a range of topics including metallurgy(alloying and processing,coatings,material properties,and corrosion),research concerned with testing in vitro and in vivo,and other important aspects of biological characterisation.

关键词： alloying corrosion southeast

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In silico modelling of the corrosion of biodegradable magnesium-based biomaterials:modelling approaches,validation and future perspectives

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Biomaterials Translational 2021年第3期2卷 257-271页

作者： Aditya Joshi George Dias mark p.staiger Department of Mechanical Engineering University of CanterburyChristchurchNew Zealand Department of Anatomy University of OtagoDunedinNew Zealand

Metallic biomedical implants based on magnesium,zinc and iron alloys have emerged as bioresorbable alternatives to permanent orthopaedic implants over the last two *** corrosion rate of biodegradable metals plays a critical role in controlling the compatibility and functionality of the device in *** broader adoption of biodegradable metals in orthopaedic applications depends on developing in vitro methods that accurately predict the biodegradation behaviour in ***,the physiological environment is a highly complex corrosion environment to replicate in the laboratory,making the in vitro-to-in vivo translation of results very ***,the results from in vitro corrosion tests fail to provide a complete schema of the biodegradation behaviour of the metal in *** silico approach based on computer simulations aim to bridge the observed differences between experiments performed in vitro and vivo.A critical review of the state-of-the-art of computational modelling techniques for predicting the corrosion behaviour of magnesium alloy as a biodegradable metal is presented.

关键词： biomaterial biodegradation corrosion finite element method magnesium modelling

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A New Set of Reversibly photoswitchable Fluorescent proteins for Use in Transgenic plants

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Molecular plant 2013年第5期6卷 1518-1530页

作者： Martina Lummer Fabian Humpert Matthias Wiedenubbert markus Sauer mark Schuttpelz Dorothee staiger Molecular Cell physiology Bielefeld University Universitatsstr. 25 33615 Bielefeld Germany Institute for Genome Research and Systems Biology CeBiTec 33615 Bielefeld Germany Biomolecular photonics Bielefeld University Universitatsstr. 25 33615 Bielefeld Germany Biotechnology and Biophysics Julius-Maximilians-University Wurzburg Am Hubland 97074 Wurzburg Germany

Fluorescent reporter proteins that allow repeated switching between a fluorescent and a non-fluorescent state in response to specific wavelengths of light are novel tools for monitoring of protein trafficking and super-resolu- tion fluorescence microscopy in living organisms. Here, we describe variants of the reversibly photoswitchable fluores- cent proteins rsFastLime, bsDronpa, and padron that have been codon-optimized for the use in transgenic Arabidopsis plants. The synthetic proteins, designated rsFastLIME-s, bsDRONpA-s, and pADRON C-s, showed photophysical properties and switching behavior comparable to those reported for the original proteins. By combining the ＇positively switchable＇ pADRON C-s with the ＇negatively switchable＇ rsFastLIME-s or bsDRONpA-s, two different fluorescent reporter proteins could be imaged at the same wavelength upon transient expression in Nicotiana benthamiana cells. Thus, co-localiza- tion analysis can be performed using only a single detection channel. Furthermore, the proteins were used to tag the RNA-binding protein AtGRp7 （Arabidopsis thaliana glycine-rich RNA-binding protein 7） in transgenic Arabidopsis plants. Because the new reversibly photoswitchable fluorescent proteins show an increase in signal strength during each pho- toactivation cycle, we were able to generate a large number of scans of the same region and reconstruct 3-D images of AtGRp7 expression in the root tip. Upon photoactivation of the AtGRp7：rsFastLIME-s fusion protein in a defined region of a transgenic Arabidopsis root, spreading of the fluorescence signal into adjacent regions was observed, indicating that movement from cell to cell can be monitored. Our results demonstrate that rsFastLIME-s, bsDRONpA-s, and pADRON C-s are versatile fluorescent markers in plants, Furthermore, the proteins also show strong fluorescence in mammalian cells including COS-7 and HeLa cells.

关键词： reversibly photoswitchable fluorescent protein codon usage Arabidopsis rsFastLIME-s bsDRONpA-s pADRON-s.

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marking RNA:m^6A writers,readers,and functions in Arabidopsis

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Journal of Molecular Cell Biology 2019年第10期11卷 899-910页

作者： Marlene Reichel Tino Koster Dorothee staiger RNA Biology and Molecular physiology Faculty of BiologyBielefeld University33615 BielefeldGermany

N^6-methyladenosine(m^6A)emerges as an important modification in eukaryotic mRNAs.m^6A has first been reported in 1974,and its functional significance in mammalian gene regulation and importance for proper development have been well *** arsenal of writer,eraser,and reader proteins accomplish deposition,removal,and interpretation of the m^6A mark,resulting in dynamic *** led to the concept of an epitranscriptome,the compendium of RNA species with chemical modification ofthe nucleobases in the cell,in analogy to the *** m^6A has long been known to also exist in plant mRNAs,proteins involved in m^6A metabolism have only recently been detected by mutant analysis,homology search,and mRNA interactome capture in the reference plant Arabidopsis *** ofthe m^6A modification causes severe developmental abnormalities of leaves and roots and altered timing of reproductive ***,m^6A modification affects viral ***,we discuss recent progress in identifying m^6A sites transcriptome-wide,in identifying the molecular players involved in writing,removing,and reading the mark,and in assigning functions to this RNA modification in *** highlight similarities and differences to m^6A modification in mammals and provide an outlook on important questions that remain to be addressed.

关键词： Arabidopsis m^6A mRNA interactome posttranscriptional RNA-binding protein

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