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Mineralized collagen-modified PMMA cement enhances bone integration and reduces fibrous encapsulation in the treatment of lumbar degenerative disc disease

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Regenerative Biomaterials 2020年第2期7卷 181-193页

作者： Long Yang Jianjun Kong Zhiye Qiu Tieliang Shang Siyu Chen Rui Zhao maria grazia raucci Xiao Yang Zhanyong Wu National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China Department of Orthopaedics Orthopaedic Hospital of XingtaiXingtai 054000China Department of Orthopedic Laboratory Xingtai Institute of OrthopaedicsXingtai 054000China Beijing Allgens Medical Science and Technology Co. LtdBeijing 102609China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China Institute of Polymers Composites and BiomaterialsNational Research Council of ItalyNaples 80125Italy

As a minimally invasive surgery,percutaneous cement discoplasty(PCD)is now contemplated to treat lumbar disc degeneration disease in elder ***,we investigated whether the osteogenic mineralized collagen(MC)modified polymethylmethacrylate(PMMA)cement could be a suitable material in PCD ***,hydrophilicity and mechanical properties of the MC-modified PMMA(PMMA-MC)was *** introduction of MC did not change the application and setting time of PMMA and was easy to be handled in minimally invasive *** of PMMA-MC was greatly improved and its elastic modulus was tailored to complement mechanical performance of bone under dynamic ***,PCD surgery in a goat model with induced disc degeneration was performed with implantation of PMMA-MC or *** months after implantation,micro-computed tomography analysis revealed a 36.4%higher circumferential contact index between PMMA-MC and bone,as compared to PMMA *** staining confirmed that the surface of PMMA-MC was in direct contact with new bone,while the PMMA was covered by fibrous *** observed gathering of macrophages around the implant was suspected to be the cause of fibrous ***,the interactions of PMMA and PMMA-MC with macrophages were investigated in *** discovered that the addition of MC could hinder the proliferation and fusion of the ***,expressions of fibroblaststimulating growth factors,insulin-like growth factor,basic fibroblast growth factor and tumor necrosis factor-b were significantly down-regulated in the macrophages cocultured with ***,the promoted osteointegration and reduced fibrous tissue formation observed with PMMA-MC material makes it a promising candidate for PCD surgery.

关键词： mineralized collagen lumbar degenerative disc disease percutaneous cement discoplasty fibrous encapsulation

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Nanofibrous polypeptide hydrogels with collagen-like structure as biomimetic extracellular matrix

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Collagen and Leather 2023年第2期5卷 81-93页

作者： Chengkun Zhao Xing Li Shaoquan Bian Weinan Zeng Alfredo Ronca Ugo D'Amora maria grazia raucci Jie Liang Yong Sun Qing Jiang Yujiang Fan Luigi Ambrosio Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan University29#Wangjiang RoadChengdu 610064People's Republic of China College of Biomedical Engineering Sichuan University29#Wangjiang RoadChengdu 610064People's Republic of China Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhen 518055People's Republic of China Orthopedic Research Institution Department of OrthopedicsWest China HospitalSichuan UniversityChengdu 610041People's Republic of China Institute of Polymers Composites and BiomaterialsNational Research Council80125 NaplesItaly

Supramolecular peptides exhibit obvious similarities with collagen fibers in terms of self-assembly characteristics,nanofibrous structure,and responsiveness to external ***,a series of supramolecular peptides were developed by altering the amino acid sequence,enabling the self-assembly of three types of 4-biphenylacetic acid(BPAA)-tripeptides into fibrous hydrogel through hydrogen bonding and n-n stacking under the influence of ion *** electron and scanning electron microscopies revealed that the diameter of the fiber within nanofibrous hydrogels was~10 and~40 nm,respectively,which was similar with the self-assembled collagen *** this reason,these hydrogels could be considered as a biomimetic extracellular ***,the gelation concentration induced by ions was even lower than 0.66 wt%,with an elastic modulus of~0.27 kPa,corresponding to a water content of 99.34 wt%.In addition,the three supramolecular hydrogels were found to be good substrates for L929 cell adhesion and MC-3T3 cell *** overall results implied that BPAA-based hydrogels have a lucrative application potential as cell carriers.

关键词： Collagen-like Supramolecular peptides Nanofibrous hydrogel Ion-induction Biomimetic extracellular matrix

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The transcription factor HSFA7b controls thermomemory at the shoot apical meristem by regulating ethylene biosynthesis and signaling in Arabidopsis

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Plant Communications 2024年第3期5卷 47-61页

作者： Sheeba John Federico Apelt Amit Kumar Ivan FAcosta Dominik Bents maria grazia Annunziata Franziska Fichtner Caroline Gutjahr Bernd Mueller-Roeber Justyna J.Olas University of Potsdam Institute of Biochemistry and BiologyKarl-Liebknecht-Straße 24-25Haus 2014476 PotsdamGermany Max Planck Institute of Molecular Plant Physiology Am Muehlenberg 114476 PotsdamGermany Laboratory of Molecular Biology Wageningen University6700 AP Wageningenthe Netherlands Center of Plant Systems Biology and Biotechnology(CPSBB) 14 St.Knyaz Boris 1 Pokrastitel Str.4023 PlovdivBulgaria Present address:Institute of Plant Biochemistry Heinrich Heine University DusseldorfUniversit€atsstr.140225 DusseldorfGermany Leibniz Institute of Vegetable and Ornamental Crops Theodor-Echtermeyer-Weg 114979 GroßbeerenGermany

The shoot apical meristem(SAM)is responsible for overall shoot growth by generating all aboveground *** research has revealed that the SAM displays an autonomous heat stress(HS)memory of a previous non-lethal HS *** the importance of the SAM for plant growth,it is essential to determine how its thermomemory is mechanistically ***,we report that HEAT SHOCK TRAN-SCRIPTION FACTOR A7b(HSFA7b)plays a crucial role in this process in Arabidopsis,as the absence of functional HSFA7b results in the temporal suppression of SAM activity after *** found that HSFA7b directly regulates ethylene response at the SAM by binding to the promoter of the key ethylene signaling gene ETHYLENE-INSENSITIVE 3 to establish ***,we demonstrated that HSFA7b regulates the expression of ETHYLENE OVERPRODUCER 1(ETO1)and ETO1-LIKE 1,both of which encode ethylene biosynthesis repressors,thereby ensuring ethylene homeostasis at the *** together,these results reveal a crucial and tissue-specic role for HSFA7b in thermomemory at the Arabidopsis SAM.

关键词： ethylene response heat stress adaptation shoot apical meristem SAM thermopriming thermomemory

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Primary carbohydrate metabolism genes participate in heat-stress memory at the shoot apical meristem of Arabidopsis thaliana

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Molecular Plant 2024年第4期17卷 676-676页

作者： Justyna Jadwiga Olas Federico Apelt maria grazia Annunziata Sheeba John Sarah Isabel Richard Saurabh Gupta Friedrich Kragler Salma Balazadeh Bernd Mueller-Roeber 不详

(Molecular Plant 14,1508-1524,September 62021)After the original publication of the above article,attentive readers identified errors in Figure 4H that needed correction as *** 4H contained the erroneous labels“10 DAP(7 DAT),”which should have each read,“7 DAP(4 DAT).”To avoid any confusion and ensure consistency across our figures,we modified some of the Col-0 images in a corrected version of Figure 4H below to resemble the exact same Col-0 images as in Figure *** details of the changes are as follows:rotation(Col-0 Primed,all timepoints;Col-0 Triggered,3 DAP and 5 DAP;Col-0 Primed/triggered,3 DAP and 5 DAP),replacement(Col-0 Triggered,7 DAP),and different cropping(Col-0 Primed/triggered,7 DAP).

关键词： Figure metabolism Prime

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Ex vivo models of retinal neurovascular diseases

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Annals of Eye Science 2022年第2期7卷 1-7页

作者： maria grazia Rossino Department of Biology University of PisaPisaItaly

Background:The ex vivo model represented by mouse retinal explants in culture is a useful experimental model to investigate the molecular mechanism involved in neurovascular diseases such as diabetic retinopathy(DR).It ensures an experimental overview with more complete respect to isolate cells and reduce problems in terms of accessibility and management with respect to in vivo *** particular,it allows the evaluation of the relationship between retinal cells in response to the typical stressors involved in DR ***:Ex vivo retinal fragments derived from 3-to 5-week-old C57BL/6J *** particular,after dissection,the retina is cut into 4 separate fragments and transferred onto inserts placed with ganglion cells *** in culture,the explants could be treated in stress conditions typical of *** particular,this study protocol describes the procedure for the preparation and the culture of retinal explants with specific metabolic stressors such as high glucose(HG),advanced glycation end product(AGE),and oxidative stress(OS).In the end,this paper provides the protocols to perform molecular analyses in order to evaluate the response of retinal explants to stress and/or neuroprotective ***:The cultured retinal explants represent an ex vivo experimental model to investigate the molecular mechanisms involved in neurovascular diseases such as ***,they could be useful to test the effect of neuroprotective compounds in response to metabolic stressors in a fewer time respect to an in vivo *** conclusion,retinal explants in culture represent a valuable experimental model to conduct further studies to better understand the pathophysiology of DR.

关键词： Retinal explants diabetic retinopathy(DR) neurovascular diseases

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Specific contribution of mannose-binding lectin murine isoforms to brain ischemia/reperfusion injury

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Cellular & Molecular Immunology 2020年第3期17卷 218-226页

作者： Laura Neglia Marco Oggioni Domenico Mercurio maria-grazia De Simoni Stefano Fumagalli1 Istituto di Ricerche Farmacologiche Mario Negri IRCCS Department of NeuroscienceMilanItaly

Mannose-binding lectin(MBL),an initiator of the lectin pathway(LP)of complement activation,is detrimental in ischemic stroke,as shown in clinical studies and rodent *** humans have one functional MBL protein,rodents have two isoforms,MBL-A and MBL-C,whose functions relative to that of human MBL are *** permit the clinical translation of preclinical data,we aimed to define the specific contributions of MBL-A and MBL-C to brain *** subjected mice with double(MBL^(−/−))or single(MBL-A^(−/−)or MBL-C^(−/−))MBL isoform depletion to transient middle cerebral artery occlusion(tMCAo).MBL^(−/−) mice had fewer neurological deficits and smaller ischemic lesions than WT ***-A^(−/−) mice had smaller lesions than WT mice and exhibited no significant behavioral defects,whereas MBL-C^(−/−) mice did not differ from WT *** induction of Mbl1 and Mbl2(the MBL-A and MBL-C genes)expression 48 h after tMCAo was similar across *** time course of Mbl1 and Mbl2 expression in WT ischemic mice showed that Mbl1 activation occurred earlier(24 h)than Mbl2 activation(48 h).The plasma levels of MBL-A and MBL-C in MBL-C^(−/−) and MBL-A^(−/−) mice,respectively,were similar to those in WT mice both at baseline and at 48 h after *** 48 h,MBL-A^(−/−)ischemic mice showed higher MBL-C levels in the brain than WT *** and MBL-C^(−/−) ischemic mice had higher LP activity in plasma and,accordingly,higher levels of C3 deposition in the brain than MBL-A^(−/−) and MBL^(−/−) *** conclusion,mice with depletion of both MBL isoforms exhibited strong protection from ischemia/reperfusion ***-A was the main contributor to injury,likely owing to its earlier activation after ischemia and more efficient activation of the complement system than MBL-C.

关键词： injury depletion likely

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Emerging actionable targets to treat therapyresistant colorectal cancers

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Cancer Drug Resistance 2022年第1期5卷 36-63页

作者： Emanuela Grassilli maria grazia Cerrito Department of Medicine and Surgery University of Milano-BicoccaMonza 20900Italy

In the last two decades major improvements have been reached in the early diagnosis of colorectal cancer(CRC)and,besides chemotherapy,an ampler choice of therapeutic approaches is now available,including targeted and *** that,CRC remains a“big killer”mainly due to the development of resistance to therapies,especially when the disease is diagnosed after it is already *** the same time,our knowledge of the mechanisms underlying resistance has been rapidly expanding which allows the development of novel therapeutic options in order to overcome *** far as resistance to chemotherapy is concerned,several contributors have been identified such as:intake/efflux systems upregulation;alterations in the DNA damage response,due to defect in the DNA checkpoint and repair systems;dysregulation of the expression of apoptotic/anti-apoptotic members of the BCL2 family;overexpression of oncogenic kinases;the presence of cancer stem cells;and the composition of the tumoral microenvironment and that of the gut ***,several mechanisms are also involved in the resistance to targeted and/or *** example,overexpression and/or hyperactivation and/or amplification of oncogenic kinases can sustain resistance to targeted therapy whereas the composition of the gut microbiota,as well as that of the tumoral niche,and defects in DNA repair systems are crucial for determining the response to *** this review we will make an overview of the main resistance mechanisms identified so far and of the new therapeutic approaches to overcome it.

关键词： Colorectal cancer resistance chemotherapy target therapy EGFR ERBB2 MET BRAF kinase inhibitors immune checkpoint inhibitors gut microbiota

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Thermal Radiation Modelling in Tunnel Fires

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Advances in Applied Mathematics and Mechanics 2011年第3期3卷 327-353页

作者： Paolo Ciambelli maria grazia Meo Paola Russo Salvatore Vaccaro Department of Industrial Engineering University of Salernovia Ponte don Melillo 84084 Fisciano(SA)Italy

Modelling based on Computational Fluid Dynamics(CFD)is by now effectively used in fire research and hazard *** on the scenario,radiative heat transfer can play a very important role in enclosure combustion events such as tunnel *** this work,the importance of radiation and the effect of the use of different approaches to account for it were ***,small-scale tunnel fire simulations were performed and the results compared with experimental data,then realistic full-scale scenarios were *** results show up the capability of CFD modelling to reproduce with good approximation tunnel *** proved to be noteworthy mainly when the scale of the fire is relatively *** the various approaches employed to simulate radiation,the use of the Discrete Transfer model gave the most accurate results,mainly when the absorption-emission characteristics of the combustion products were taken into ***,the suitability of the use of CFD in quantitative Fire Hazard Analysis is discussed.

关键词： CFD radiation modelling fire hazard analysis tunnel fires

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Prolonged exposure of colon cancer cells to the epidermal growth factor receptor inhibitor gefitinib(Iressa^(TM))and to the antiangiogenic agent ZD6474:Cytotoxic and biomolecular effects

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World Journal of Gastroenterology 2006年第32期12卷 5140-5147页

作者： Amalia Azzariti Letizia Porcelli Jian-Ming Xu grazia maria Simone Angelo Paradiso Clinical Experimental Oncology Laboratory National Cancer Institute Bari Italy Beijing 307 Hospital Cancer Center Beijing 100039 China

AIM： To analyze the biological effects of prolonged in vitro exposure of HT-29 and LoVo colon cancer cell lines to gefitinib （Iressa^TM）, an inhibitor of epidermal growth factor receptor （EGFR） activity, and ZD6474, an inhibitor of both KDR and EGFR activities. METHODS： Cells were treated with each drug for up to 2 wk using either a continuous or an intermittent （4 d of drug exposure followed by 3 d of washout each week） schedule. RESULTS： In both cell types, prolonged exposure （up to 14 d） to gefitinib or ZD6474 produced a similar inhibition of cell growth that was persistent and independent of the treatment schedule. The effects on cell growth were associated with a pronounced inhibition of p-EGFR and/ or p-KDR expression. Treatment with gefitinib or ZD6474 also inhibited the expression of EGFR downstream signal molecules, p-Erkl/2 and p-Akt, although the magnitude of these effects varied between treatments and cell lines. Furthermore, expression of the drug resistance-related protein ABCG2 was shown to significantly increase after 14 d of continuous exposure to the two drugs. CONCLUSION： We conclude that long-term exposure of colon cancer cells to gefitinib and ZD6474 does not modify their cytotoxic effects but it might have an effect on sensitivity to classical cytotoxic drugs.

关键词： Gefitinib ZD6474 Colon cancer Tyrosine ki-nase Chemo-resistance

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pFTAA: a high affinity oligothiophene probe that detects filamentous tau in vivo and in cultured neurons

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Neural Regeneration Research 2015年第11期10卷 1746-1747页

作者： Jack Brelstaff maria grazia Spillantini Aviva M.Tolkovsky Department of Clinical Neurosciences The Clifford Allbutt Building University of Cambridge

Tauopathies describe a group of neurodegenerative diseases in which the protein tau,encoded by the gene MAPT,is aberrantly misfolded,leading to tau aggregation,neural dysfunction,and cell death（Spillantini and Goedert,2013）.In Alzheimer＇s disease（AD）,tau forms the characteristic intracellular neurofibrillary tangles（NFTs）,which are thought to be the major cause of neurodegeneration（Bloom,2014）.In other tauopathies,including frontotemporal dementia with Parkinsonism linked to chromo- some 17 （FTDP-17T）, corticobasal degeneration and progressive supranuclear palsy, there are specific forms of tau aggregates and filaments without any amyloid pathology, demonstrating tau＇s po- tent disease-causing potential （Spillantini and Goedert, 2013）. Tau is a microtubule （MT） binding protein, which becomes abnormally hyperphosphorylated on several residues prior/during the process of aggregation, thereby causing loss of its MT binding activity （Mandelkow and Mandelkow, 2012）.

关键词： pFTAA a high affinity oligothiophene probe that detects filamentous tau in vivo and in cultured neurons high

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