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Venous Catheterization Experimental Model in Rabbits:Histological Alterations in the Catheter Region

Surgical Science 2015年第12期6卷 532-540页

作者： Bianca Ribeiro Rodrigues Gabriela Samaha Franca maria cecilia ferro Canabarro Luciana Willy Marcus Franca Medical Science and Health Faculty Catholic University of Sao Paulo(FCMSPUC/SP)Sao PauloBrazil University City of Sao Paulo(UNICID) Sao PauloBrazil Faculdade de Ciencias Médicas e da Saúde(FCMS) Pontifícia Universidade Catolica de Sao Paulo(PUC/SP)Sao PauloBrazil

Central venous access is one of the most common surgery procedures worldwide, especially in pediatric surgery. Local and regional complications as the result of venous catheter permanence time are frequently described as: thrombosis, infection, edema and local cellulite, movement and loss of the catheter. Other severe complications such as endocardiac and hemorrhagic lesions are also described and considered the cause of catheter early removal. In the literature few studies have addressed vascular and perivascular lesions and complications as the result of central venous access to peripheral veins, given the difficulty of setting up venous catheterization experimental models to study blood vessels and perivascular tissue alterations after catheterization. In the present venous catheterization experimental model, rabbits were divided into two groups based on the time that the venous catheters were maintained in their veins. Group a composed of 7 New Zealand male rabbits was submitted to a 15-day treatment;and the 6 New Zealand male rabbits of group B were treated during 90 days. Both groups presented similar inflammatory conditions since there was no significant difference between groups. Therefore, the results may well suggest that the endothelial inflammatory reaction could have developed at an early initial short period and by maintaining the catheter, the inflammatory reactions would have decreased or disappeared. Aimed at studying these vascular and perivascular alterations in venous catheterization, the present study proposes an experimental rabbit model that allows the analysis of differences in local vascular and perivascular histological variations and compares histological differences between both venous catheterization groups each of them with different periods of treatment.

关键词： Experimental Surgery Central Venous Catheter Venous Thrombosis Phlebitis

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Antioxidant treatment for oligoasthenoteratozoospermia and varicocele:a DBPC trial to evaluate the impact of age and body mass index

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Asian Journal of Andrology 2024年第3期26卷 239-244页

作者： Gian maria Busetto Bernarde F Rodrigues Ashraf Virmani Andrea Checchia Antonella Ninivaggi Anna Ricapito Giovanni Barbieri Piero Fischetti Ugo G Falagario Pasquale Annese Nicola d’Altilia Vito Mancini Matteo ferro Felice Crocetto Angelo Porreca Carlo Bettocchi Luigi Cormio Ashok Agarwal Giuseppe Carrieri University of Foggia Foggia 71122Italy Department of Urology and Renal Transplantation Policlinico of FoggiaFoggia 71122Italy Department of Urology Human Reproduction SectionFederal University of Sao PauloSao Paulo 05403-000Brazil Alfasigma Healthscience Utrecht 3528 BGThe Netherlands Urology Unit “G.Tatarella”HospitalCerignola 71042Italy Urology Unit European Institute of Oncology(IEO)IRCCSMilan 20142Italy University of Naples Federico II Naples80126Italy Department of Oncological Urology Veneto Institute of Oncology(IOV)IRCCSPadua 35039Italy Urology Unit “L.Bonomo”HospitalAndria 70031Italy Global Andrology Forum Moreland HillsOH 44022USA

Oxidative stress is one of the main mechanisms responsible for male *** conditions such as varicocele,obesity,advanced age,and lifestyle can lead to an increase in reactive oxygen species,causing an oxidative imbalance in the *** are sensitive to reactive oxygen species and require energy to carry out their main function of fertilizingthe *** reactive oxygen species can affect sperm metabolism,leading to immobility,impaired acrosome reaction,and celldeath,thereby impairing reproductive *** double-blind randomized study evaluated the effect of supplementation withL-carnitine,acetyl-L-carnitine,vitamins,and other nutrients on semen quality in 104 infertile patients with or without varicocele,while also investigating the impact of factors such as obesity and advanced age on *** concentration significantlyincreased in the supplemented group compared to the placebo group(P=0.0186).Total sperm count also significantly increasedin the supplemented group(P=0.0117),as did sperm motility(P=0.0120).The treatment had a positive effect on patients upto 35 years of age in terms of sperm concentration(P=0.0352),while a body mass index(BMI)above 25 kg m^(−2) had a negativeeffect on sperm concentration(P=0.0110).Results were not showing a net benefit in stratifying patients in accordance withtheir BMI since sperm quality increase was not affected by this *** conclusion,antioxidant supplementation may bebeneficial for infertile patients and has a more positive effect on younger patients with a normal weight.

关键词： aging antioxidants body mass index oligoasthenoteratozoospermia varicocele

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Minimally invasive surgical therapies(MISTs)for lower urinary tract symptoms(LUTS):promise or panacea?

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Asian Journal of Andrology 2024年第2期26卷 135-143页

作者： Gian maria Busetto Andrea Checchia Marco Recchia Edoardo Tocci Ugo G Falagario Gennaro Annunziata Pasquale Annese Nicola d'Altilia Vito Mancini Matteo ferro Felice Crocetto Octavian Sabin Tataru Luca Di Gianfrancesco Angelo Porreca Francesco Del Giudice Ettore De Berardinis Carlo Bettocchi Luigi Cormio Giuseppe Carrieri University of Foggia Foggia 71122Italy Department of Urology and Renal Transplantation Policlinico of FoggiaFoggia 71122Italy Urology Unit "G.Tatarlla"HospitalCerignola 71042Italy Urology Unit European Institute of Oncology(IE0)IRCcSMilan 20141Italy University of Naples Federico Il Naples 80126Italy G.E.Palade University of Medicine PharmacyScienceand Technology of Targu MuresTargu Mures 540142Romania Department of Oncological Urology Veneto Institute of Oncology(IOv)IRccSPadua 35128Italy Department of Maternal-Infant and Urological Sciences Sapienza Rome UniversityRoma 00161Italy Urology Unit "L.Bonomo"HospitalAndria 76123Italy

The increasing importance of treatment of lower urinary tract symptoms(LUTS),while avoiding side effects and maintaining sexual function,has allowed for the development of minimally invasive surgical therapies(MiSTs).Recently,the European Association of Urology guidelines reported a paradigm shift from the management of benign prostatic hyperplasia(BPH)to the management of nonneurogenic male *** aim of the present review was to evaluate the efficacy and safety of the most commonly used MisTs:ablative techniques such as aquablation,prostatic artery embolization,water vapor energy,and transperineal prostate laser ablation,and nonablative techniques such as prostatic urethral lift and temporarily implanted nitinol device(iTIND).MiSTs are becoming a new promise,even if clinical trials with longer follow-up are still *** of them are still under investigation and,to date,only a few options have been given as a recommendation for *** cannot be considered as standard of care and are not suitable for all *** and disadvantages should be underlined,without forgetting our objective:treatment of LUTS and re-treatment avoidance.

关键词： aquablation iTIND prostatic artery embolization prostatic urethral lift transperineal prostate laser ablation water vapor energy

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This pain drives me crazy:Psychiatric symptoms in women with interstitial cystitis/bladder pain syndrome

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World Journal of Psychiatry 2024年第6期14卷 954-984页

作者： marianna Mazza Stella Margoni Giuseppe Mandracchia Guglielmo Donofrio Alessia Fischetti Georgios D Kotzalidis Giuseppe Marano Alessio Simonetti Delfina Janiri Lorenzo Moccia Ilaria Marcelli Greta Sfratta Domenico De Berardis Ottavia Ferrara Evelina Bernardi Antonio Restaino Francesco maria Lisci Antonio maria D'Onofrio Caterina Brisi Flavia Grisoni Claudia Calderoni Michele Ciliberto Andrea Brugnami Sara Rossi maria Chiara Spera Valeria De Masi Ester maria Marzo Francesca Abate Gianluca Boggio maria Benedetta Anesini cecilia Falsini Anna Quintano Alberto Torresi Miriam Militenda Giovanni Bartolucci Marco Biscosi Sara Ruggiero Luca Lo Giudice Giulia Mastroeni Elisabetta Benini Luca Di Benedetto Romina Caso Francesco Pesaresi Francesco Traccis Luca Onori Luca Chisari Leonardo Monacelli mariateresa Acanfora Eleonora Gaetani Monia Marturano Sara Barbonetti Elettra Specogna Francesca Bardi Emanuela De Chiara Gianmarco Stella Andrea Zanzarri Flavio Tavoletta Arianna Crupi Giulia Battisti Laura Monti Giovanni Camardese Daniela Chieffo Antonio Gasbarrini Giovanni Scambia Gabriele Sani Department of Neurosciences Fondazione Policlinico Universitario A Gemelli IRCCSUniversita Cattolica del Sacro CuoreRome 00168Italy Department of Psychiatry UniversitàCattolica del Sacro CuoreRome 00168Italy Department of Neurosciences Section of PsychiatryFondazione Policlinico Universitario Agostino Gemelli IRCCSUniversitàCattolica del Sacro CuoreRome 00168Italy Mental Health NHSASL 4 TeramoTeramo 64100Italy Medical and Surgical Sciences UniversitàCattolica del Sacro CuoreRome 00168Italy Division of Gynecologic Oncology UniversitàCattolica del Sacro CuoreRome 00168Italy UOS Psicologia Clinica Governo ClinicoFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy CEMAD Digestive Disease Center Fondazione Policlinico Universitario A.Gemelli-IRCCSRome 00168Italy Department of Woman and Child Health Catholic UniversityRome 00168Italy UOC Psichiatria Clinica e d’Urgenza Dipartimento di Scienze Dell’InvecchiamentoNeurologicheOrtopediche e Della Testa-colloFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy

BACKGROUND Interstitial cystitis/bladder pain syndrome(IC/BPS)is an at least 6-mo noninfectious bladder inflammation of unknown origin characterized by chronic suprapubic,abdominal,and/or pelvic *** the term cystitis suggests an inflammatory or infectious origin,no definite cause has been *** occurs in both sexes,but women are twice as much *** To systematically review evidence of psychiatric/psychological changes in persons with IC/*** Hypothesizing that particular psychological characteristics could underpin IC/BPS,we investigated in three databases the presence of psychiatric symptoms and/or disorders and/or psychological characteristics in patients with IC/BPS using the following strategy:("interstitial cystitis"OR"bladder pain syndrome")AND("mood disorder"OR depressive OR antidepressant OR depression OR depressed OR hyperthymic OR mania OR manic OR rapid cyclasterisk OR dysthymiasterisk OR dysphoriasterisk).RESULTS On September 27,2023,the PubMed search produced 223 articles,CINAHL 62,and the combined PsycLIT/PsycARTICLES/PsycINFO/Psychology and Behavioral Sciences Collection search *** on *** produced 14 studies,of which none had available *** were peer-reviewed articles reporting psychiatric/psychological symptoms in patients with IC/BPS,i.e.63 articles spanning from 2000 to October *** studies identified depression and anxiety problems in the IC/BPS population,along with sleep problems and the tendency to *** Psychotherapies targeting catastrophizing and life stress emotional awareness and expression reduced perceived pain in women with IC/*** concepts should be considered when implementing treatments aimed at reducing IC/BPS-related pain.

关键词： Interstitial cystitis/bladder pain syndrome Psychiatric symptoms Psychological symptoms Catastrophizing Anxiety Depression

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Reduced lysosomal acid lipase activity: A new marker of liver disease severity across the clinical continuum of non-alcoholic fatty liver disease?

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World Journal of Gastroenterology 2019年第30期25卷 4172-4180页

作者： Francesco Baratta Daniele Pastori Domenico ferro Giovanna Carluccio Giulia Tozzi Francesco Angelico Francesco Violi maria Del Ben Department of Internal Medicine and Medical Specialties Sapienza-University of RomeRome 00155Italy Hepatogastroenterology and Nutrition Unit-Pediatric Department Bambino GesùChildren’s HospitalRome 00156Italy Department of Public Health and Infectious Disease Sapienza-University of RomeRome 00161Italy

Lysosomal acid lipase(LAL)plays a key role in intracellular lipid *** LAL activity promotes increased multi-organ lysosomal cholesterol ester storage,as observed in two recessive autosomal genetic diseases,Wolman disease and Cholesterol ester storage *** liver steatosis and accelerated liver fibrosis are common features in patients with genetic LAL *** contrast,few reliable data are available on the modulation of LAL activity in vivo and on the epigenetic and metabolic factors capable of regulating its activity in subjects without homozygous mutations of the Lipase A *** the last few years,a less severe and non-genetic reduction of LAL activity was reported in children and adults with non-alcoholic fatty liver disease(NAFLD),suggesting a possible role of LAL reduction in the pathogenesis and progression of the *** with NAFLD show a significant,progressive reduction of LAL activity from simple steatosis to non-alcoholic steatohepatitis and cryptogenic *** cirrhosis of different etiologies,those with cryptogenic cirrhosis show the most significant reductions of LAL *** findings suggest that the modulation of LAL activity may become a possible new therapeutic target for patients with more advanced forms of ***,the measurement of LAL activity may represent a possible new marker of disease severity in this clinical setting.

关键词： Non-alcoholic fatty liver disease Non-alcoholic steatohepatitis Lysosomal acid lipase Cirrhosis Wolman disease Cholesterol ester storage disease

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Hypertension in kidney transplant recipients

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World Journal of Transplantation 2022年第8期12卷 211-222页

作者： maria-Eleni Alexandrou Charles J ferro Ioannis Boletis Aikaterini Papagianni Pantelis Sarafidis Department of Nephrology Hippokration HospitalAristotle University of ThessalonikiThessaloniki 54642Greece Department of Renal Medicine University Hospitals Birmingham NHS Foundation TrustBirmingham B152WBUnited Kingdom Department of Nephrology Laiko General HospitalNational and Kapodistrian UniversityAthens 11527Greece

Kidney transplantation is considered the treatment of choice for end-stage kidney disease ***,the residual cardiovascular risk remains significantly higher in kidney transplant recipients(KTRs)than in the general *** is highly prevalent in KTRs and represents a major modifiable risk factor associated with adverse cardiovascular outcomes and reduced patient and graft *** definition of hypertension and recognition of special phenotypes and abnormal diurnal blood pressure(BP)patterns is crucial for adequate BP *** by office BP is commonly encountered in these patients,and a high proportion of masked and uncontrolled hypertension,as well as of white-coat hypertension,has been revealed in these patients with the use of ambulatory BP *** pathophysiology of hypertension in KTRs is multifactorial,involving traditional risk factors,factors related to chronic kidney disease and factors related to the transplantation *** the absence of evidence from large-scale randomized controlled trials in this population,BP targets for hypertension management in KTR have been extrapolated from chronic kidney disease *** most recent Kidney Disease Improving Global Outcomes 2021 guidelines recommend lowering BP to less than 130/80 mmHg using standardized BP office *** calcium channel blockers and angiotensin-converting enzyme inhibitors/angiotensin-II receptor blockers have been established as the preferred first-line agents,on the basis of emphasis placed on their favorable outcomes on graft *** aim of this review is to provide previous and recent evidence on prevalence,accurate diagnosis,pathophysiology and treatment of hypertension in KTRs.

关键词： Hypertension Kidney transplantation Epidemiology Diagnosis Physiopathology Therapy

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A Footprint of Plant Desiccation Tolerance. Does It Exist？

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Molecular Plant 2018年第8期11卷 1003-1005页

作者： Henk W.M. Hilhorst maria-cecilia D. Costa Jill M. Farrant Laboratory of Plant Physiology Wageningen University and Research Droevendaalsesteeg 1 Wageningen 6708 PB the Netherlands Department of Molecular and Cell Biology University of Cape Town Private Bag Rondebosch Cape Town 7701 South Africa

Desiccation tolerance （DT）, the ability to survive loss of more than 90% of cellular water （≤0.1 g H2O g^-1 dry mass） for prolonged periods, is common in seeds （90% of angiosperm species） but rare in vegetative tissues （0.04% of land plants; reviewed in Gaff and Oliver, 2013）. Vegetative DT is more widespread in non-tracheophytes, being present in 25 species of algae, 158 bryophytes species, and all lichens, but is rare in tracheophytes, being displayed in 64 pteridophyte and 135 angiosperm species （Gaff and Oliver, 2013）.

关键词： RESURRECTION EVOLUTION GENOME

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A systematic review and meta-analysis of clinical trials implementing aromatase inhibitors to treat male infertility

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Asian Journal of Andrology 2020年第4期22卷 360-367页

作者： Francesco Del Giudice Gian maria Busetto Ettore De Berardinis Isabella Sperduti Matteo ferro Martina Maggi Martin S Gross Alessandro Sciarra Michael L Eisenberg Department of Urology Sapienza Rome UniversityPoliclinico Umberto IRome 00161Italy Biostatistical Unit IRCCSRegina Elena National Cancer InstituteRome 00128Italy Division of Urology European Institute of OncologyMilan 20141Italy Section of Urology Dartmouth-Hitchcock Medical CenterLebanonNH 03766USA Department of Urology Stanford UniversitySchool of MedicineStanfordCA 94305USA

Aromatase activity has commonly been associated with male infertility characterized by testicular dysfunction with low serum testosterone and/or testosterone to estradiol *** this subset of patients,and particularly in those with hypogonadism,elevated levels of circulating estradiol may establish a negative feedback on the hypothalamic–pituitary–testicular axis by suppressing follicle-stimulating hormone(FSH)and luteinizing hormone(LH)production and impaired *** manipulation via different agents such as selective estrogen modulators or aromatase inhibitors to increase endogenous testosterone production and improve spermatogenesis in the setting of infertility is an off-label option for *** carried out a systematic review and meta-analysis of the literature of the past 30 years in order to evaluate the benefits of the use of aromatase inhibitors in the medical management of infertile/hypoandrogenic ***,eight original articles were included and critically *** steroidal(Testolactone)or nonsteroidal(Anastrozole and Letrozole)aromatase inhibitors were found to statistically improve all the evaluated hormonal and seminal outcomes with a safe tolerability *** the evidence is promising,future prospective randomized placebo-controlled multicenter trials are necessary to better define the efficacy of these medications.

关键词： aromatase inhibitor hypogonadism male infertility meta-analysis systematic review

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IFN-γ upregulates membranous and soluble PD-L1 in mesothelioma cells: potential implications for the clinical response to PD-1/PD-L1 blockade

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Cellular & Molecular Immunology 2020年第4期17卷 410-411页

作者： maria Pia Pistillo Roberta Carosio Barbara Banelli Anna Morabito Luca Mastracci Paola ferro Serena Varesano Roberta Venè Alessandro Poggi Silvio Roncella Tumor Epigenetics Unit IRCCS Ospedale Policlinico San Martino16132 GenoaItaly Department of Surgical Sciences and Integrated Diagnostics(DISC) University of Genoa16132 GenoaItaly Anatomic Pathology IRCCS Ospedale Policlinico San Martino16132 GenoaItaly Histopathology and Cytopathology Division Azienda Sanitaria Locale 519121 La SpeziaItaly Molecular Oncology and Angiogenesis Unit IRCCS Ospedale Policlinico San Martino16132 GenoaItaly AIL Associazione italiana contro le leucemielinfomi e mieloma19121 La SpeziaItaly

Programmed death-ligand-1(PD-L1)plays a crucial role in the suppression of the antitumor immune response upon interaction with programmed cell death protein-1(PD-1)on cytotoxic T ***-L1 is constitutively expressed on antigen-presenting cells or tumor ***,PD-L1 has been detected by immunohistochemistry in a variety of tumors,including malignant pleural mesothelioma(MPM),in which it has emerged as a predictive biomarker for PD-1/PD-L1 immune checkpoint blockers(ICBs).1,2 PD-L1 expression on tumor cells can be upregulated by several factors including the interferon gamma(IFN-γ)cytokine produced by tumor-infiltrating lymphocytes(TILs).3 In MPM cells,it has been reported that IFN-γupregulates PD-L1 mRNA expression due to the activation of the interferon regulatory factor 1(IRF1)transcription factor,4 but no data have been shown regarding cell surface PD-L1 that is functionally relevant for its contact with PD-1-positive cells.

关键词： interferon death pleural

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5-ASA colonic mucosal concentrations resulting from different pharmaceutical formulations in ulcerative colitis

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World Journal of Gastroenterology 2013年第34期19卷 5665-5670页

作者： Renata D’Incà Martina Paccagnella Romilda Cardin Surajit Pathak Vincenzo Baldo maria cecilia Giron Giacomo Carlo Sturniolo Department of Surgical Oncological and Gastroenterological SciencesUniversity of Padua Department of Environmental Medicine and Public Health Institute of HygieneUniversity of Padua Department of Pharmacology and Anaesthesiology University of Florence

AIM:To compare the mucosal concentrations of 5-aminosalicylic acid(5-ASA)resulting from different pharmaceutical formulations and analyse the influence of inflammation on the mucosal ***:The study included 130 inflammatory bowel disease(IBD)patients receiving 5-ASA as pH-dependent-release formulations(73 patients),time-dependent-release formulations(11 patients),or pro-drugs(18patients).In addition,28 patients were receiving topical treatment(2-4 g/d)with pH-dependent-release *** biopsies were obtained from the sigmoid region during the *** 5-ASA concentrations(ng/mg)were measured in tissue homogenatesusing high-pressure liquid chromatography with electrochemical *** t test and Mann-Whitney test,when appropriate,were used for statistical ***:Patients receiving pH-dependent-release formulations showed significantly higher mucosal concentrations of 5-ASA(51.75±5.72 ng/mg)compared with patients receiving pro-drugs(33.35±5.78 ng/mg,P=0.01)or time-dependent-release formulations(38.24±5.53 ng/mg,P=0.04).Patients with endoscopic remission had significantly higher mucosal concentrations of5-ASA than patients with active disease(60.14±7.95ng/mg vs 35.66±5.68 ng/mg,P=0.02).Similar results were obtained when we compared patients with the histological appearance of remission and patients with active histological inflammation(67.53±9.22 ng/mg vs 35.53±5.63 ng/mg,P<0.001).Significantly higher mucosal concentrations of 5-ASA were detected in patients treated with both oral and topical treatments in combination compared with patients who received oral treatment with pH-dependent-release formulations alone(72.33±11.23 ng/mg vs 51.75±5.72 ng/mg,P=0.03).CONCLUSION:IBD patients showed significant variability in mucosal 5-ASA concentrations depending on the type of formulation,and the highest mean concentration was achieved using pH-dependent-release formulations.

关键词： 5-aminosalicylic acid Inflammatory bowel diseases Mucosal concentration

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