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The effect of high fat food on erythrocyte phospholipids, fatty acids composition and glutathione redox-system of rats with alimentary dyslipidemia

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Health 2010年第1期2卷 45-50页

作者： Natalia v. zhukova Yulia K. Karaman Natalia v. zhukova 不详

To evaluate the effects of high fat food consisted of tallow (19% of total diets) and cholesterol (2%) on modification of erythrocyte phospholipids, fatty acids composition and glutathione redox- system of male Wistar rats with alimentary dysli- pidemia. The results demonstrated that after 30 and 180 days of high-fat feed erythrocyte phos- phatidylinositol and phosphatidylcholine levels were reduced, phosphatidylserine were in-creased. Only on the 90 days of the experiment phosphatidylinositol level increased. In all grow- ups the erythrocyte 18:0 saturated fatty acids and 20:4n6, 22:4n6 polyunsaturated fatty acids (PUFA) were increased. Deficit of n3 PUFA- 20:5n3 and 22:6n3 after 90 and 180 days high fat feed promoted compensatory synthesis from 18:1n9 on 20:3n9. Erythrocyte maleic dialde-hyde increased, glutathione level decreased in all groups of rats after fed with high-fat feed. Glutathione reductase and glutathione peroxi-dase activity decreased in erythrocytes after 30 and 180 days of high-fat feed. In conclusion: high-fat diet during 30-90 days started adaptive answer in lipids of membrane and glutathione redox-system. Important mechanism of adapta-tion of a cellular membrane to high-fat diet is increase major, structuring a membrane phos-phatidylethanolamine and minor, most meta-bolic significant fractions phospholipids (phos- phatidylinositol), keeps homeostasis of 18:2n6 and 22:6n3, 20:3n9 compensatory synthesis, decrease in activity of processes lipid peroxi-dation, activation of enzymes of redox-system glutathione. But prolonging the high-fat feeding (180 days and more) formed failure compensa-tory processes (dysadaptation). It is a risk factor of developmening atherosclerosis, diabetes, steatogepatitis and other diseases.

关键词： Fatty Acids Phospholipids Adaptation Dyslipidemia GSH Glutathione Reductase Glutathione Peroxidase

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Stable oligonucleotide-functionalized gold nanosensors for environmental biocontaminant monitoring

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Journal of Environmental Sciences 2017年第12期29卷 49-59页

作者： maria v.Riquelme Weinan Leng Marcos Carzolio Amy Pruden Peter vikesland via Department of Ciuil ancl Environmental Engineering Virginia TechBlacksburgVA 24061United States Department of Statistics Virginia TechBlacksburgVA 24061United States

The global propagation of environmental biocontaminants such as antibiotic resistant pathogens and their antibiotic resistance genes（ARGs） is a public health concern that highlights the need for improved monitoring strategies. Here, we demonstrate the environmental stability and applicability of an oligonucleotide-functionalized gold nanosensor. The mec A ARG was targeted as model biocontaminant due to its presence in clinically-relevant pathogens and to its emergence as an environmental *** A-specific nanosensors were tested for antibiotic resistance gene（ARG） detection in ARG-spiked effluent from four wastewater treatment plants（WWTPs）. The mec A-specific nanosensors showed stability in environmental conditions and in high ionic strength（[MgCl_2] 〈 50 m M）, and high selectivity against mismatched targets. Spectrophotometric detection was reproducible with an LOD of 70 pM（≈ 4 × 10~7 genes/μL）, even in the presence of interferences associated with non-target genomic DNA and complex WWTP effluent. This contribution supports the environmental applicability of a new line of cost-effective, field-deployable tools needed for wide-scale biocontaminant monitoring.

关键词： Stable oligonucleotide-functionalized gold nanosensors for environmental biocontaminant monitoring LOD DNA

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Ion-conducting Membranes Based on Bacterial Cellulose Nanofibers Modified by Poly(sodium acrylate-co-2-acrylamido-2-methylpropanesulfonic acid)

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Chinese Journal of Polymer Science 2024年第3期42卷 333-343,I0007页

作者： Elizaveta v.Batishcheva Nikolay N.Smirnov Natalya v.Bobrova maria P.Sokolova Michael A.Smirnov Institute of Macromolecular Compounds Russian Academy of SciencesBolshoy pr.31Saint Petersburg 199004Russia

Green method for preparation of ion-conducting membranes(ICM) based on bacterial cellulose nanofibers(CNF) modified by a copolymer of sodium acrylate and 2-acrylamido-2-methylpropanesulfonic acid was elaborated. FTIR and NMR data confirmed grafting of polyacrylate onto cellulose surface. Formation of porous structure of the ICM was controlled by SEM and AFM. The maximal ionic conductivity of the membranes reaches 1.5 and 3.1 mS·cm^(-1)(60 ℃ and 98% relative humidity) when they are saturated with water or H_2SO_4(1 mol·L^(-1)) electrolyte,respectively. Prepared ICM was tested as a separator in a symmetrical supercapacitor with electrodes based on polyaniline hydrogel. The assembled cell demonstrate ability to operate at high current density up to 100 A·g^(-1) maintaining specific capacitance 165 F·g^(-1). Maximal specific capacitance of 289 F·g^(-1) was achieved at current density 1 A·g^(-1). Retaining of 90% of initial capacitance after 10000 of charge-discharge cycles proves high electrochemical stability of prepared ICM.

关键词： Bacterial cellulose Ion conductivity Porous membrane Supercapacitor

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Histological assessment in peripheral nerve tissue engineering

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Neural Regeneration Research 2014年第18期9卷 1657-1660页

作者： víctor Carriel Ingrid Garzón Miguel Alaminos maria Cornelissen Department of Histology (Tissue Engineering Group) Faculty of MedicineUniversity of Granadaand Instituto de Investigación Biosanitariaibs Department of Basic Medical Sciences (Histology and Tissue Engineering Group) Faculty of MedicineGhent University

The histological analysis of peripheral nerve regeneration is one of the most used methods to demonstrate the success of the regeneration through nerve conduits. Nowadays, it is possible to evaluate different parameters of nerve regeneration by using histological, histochemical, immunohistochemical and ultrastructural techniques. The histochemical methods are very sensible and are useful tools to evaluate the extracellular matrix remodeling and the myelin sheath, but they are poorly specific. In contrast, the immunohistochemical methods are highly specific and are frequently used for the identification of the regenerated axons, Sehwann cells and proteins associated to nerve regeneration or neural linage. The ultrastructural techniques offer the possibility to perform a high resolution morphological and quantitative analysis of the nerve regeneration. However, the use of a single histological method may not be enough to assess the degree of regeneration, and the combination of different histological techniques could be necessary.

关键词： peripheral nerve regeneration histology, histochemistry immunohistochemistry quantitative histology

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Flat Absorber Phosphorous Black Nickel Coatings for Space Applications

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Journal of Materials Science & Technology 2012年第2期28卷 118-124页

作者： v.maria Shalini P.Arockiasamy R.Uma Rani A.K.Sharma Thermal Systems Group ISRO Satellite CentreVimanapura PostBangalore-560 017India

A new process of flat absorber black nickel alloy coating tion from a bath containing nickel, zinc and ammonium was developed on stainless steel by electrodeposi- sulphates; thiocyanate and sodium hypophosphite for space applications. Coating process was optimized by investigating the effects of plating parameters, viz concentration of bath constituents, current density, temperature, pH and plating time on the optical properties of the black deposits. Energy dispersive X-ray spectroscopy showed the inclusion of about 6% phosphorous in the coating. The scanning electron microscopy studies revealed the amorphous nature of the coating. The corrosion resistance of the coatings was evaluated by the electrochemical impedance spectroscopy （EIS） and linear polarization （LP） techniques. The results revealed that, phosphorous addition confers better corro- sion resistance in comparison to conventional black nickel coatings. The black nickel coating obtained from hypophosphite bath provides high solar absorptance （αs） and infrared emittance （εIR） of the order of 0.93. Environmental stability to space applications was established by the humidity and thermal cycling tests.

关键词： Phosphorous black nickel alloy Electrochemical impedance spectroscopy Linear polarization Solar absorptance Infrared emittance

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Carcinosarcoma of the breast:Facing the challenge of a rare nosologic entity

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World Journal of Clinical Cases 2025年第2期13卷 14-20页

作者： Aikaterini Mastoraki maria Tsamopoulou Foivos-Konstantinos Stamatis Alexios Strimpakos Ero Mouchtouri Christiana Panagi Evgenia Mela Sotiria Mastoraki Aristotelis Kechagias Dimitrios Schizas Department of Surgery National and Kapodistrian University of AthensLaikon General HospitalAthens 11527Greece Department of Surgery National and Kapodistrian University of AthensAthens 11527Greece Department of Medical Oncology Hygeia HospitalAthens 15123Greece Department of Radiology Henry Dunant Hospital CenterAthens 11526Greece Department of Surgery Athens Metropolitan HospitalAthens 15562Greece

Carcinosarcoma(CS),also known as metaplastic breast carcinoma with mesenchymal differentiation,is one of the five distinct subtypes of metaplastic breast *** is considered as a mixed,biphasic neoplasm consisting of a carcinomatous component combined with a malignant nonepithelial element of mesenchymal origin without an intermediate transition *** cellular origin of this neoplasm remains controversial,most researchers declare that neoplastic cells derive from a cellular structure with potential biphasic *** recent research on the therapeutic strategies against CS neoplastic disorders,surgical resection appears the only potentially curative *** CS metastasize by the lymphatic route,axillary assessment with sentinel lymph node biopsy and/or axillary lymph node dissection is always ***,the tumor also presents a hematogenous metastatic pattern including pleural,pulmonary,liver,brain and less commonly bone ***,surgical removal of breast CS does not necessarily ensure patient’s long-term ***,alternative therapies,such as radio-and chemotherapy proved insufficient and 5-year survival rate is ***,there is evidence that following surgery,the combination of radio and chemotherapy is associated with a better prognosis than either treatment *** aim of this review is to evaluate the results of surgical treatment for breast CS with special reference to the extent of its histological *** features,histogenesis,morphological and immunochemical findings are discussed,while the role of current diagnostic and therapeutic management of this aggressive neoplasm is emphasized.

关键词： Carcinosarcoma Breast Diagnostic approach Therapeutic management Prognostic parameters

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Temperature and precipitation,but not geographic distance,explain genetic relatedness among populations in the perennial grass Festuca rubra

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Journal of Plant Ecology 2019年第4期12卷 730-741页

作者： mariaŠurinová věroslava Hadincová vigdis vandvik Zuzana Münzbergová Department of Botany Faculty of ScienceCharles UniversityPragueCzech Republic Institute of Botany Academy of Sciences of the Czech RepublicPrůhoniceCzech Republic Department of Biology University of BergenBergenNorway Bjerknes Centre for Climate Research BergenNorway

Aims Knowledge of genetic structure of natural populations and its determinants may provide key insights into the ability of species to adapt to novel *** many genetic studies,the effects of climate could not be disentangled from the effects of geographic *** aimed to understand the effects of temperature and moisture on genetic diversity of populations and separate these effects from the effects of geographic *** also wanted to explore the patterns of distribution of genetic diversity in the system and assess the degree of clonality within the *** also checked for possible genome size variation in the *** We studied genetic variation within and among 12 populations of the dominant grass Festuca rubra distributed across a unique regional-scale climatic grid in western Norway,Europe and explored the importance of temperature,precipitation and geo-graphic distance for the observed *** also explored the distribution of genetic diversity within and among popula-tions,identified population differentiation and estimated degree of *** analyses used microsatellites as the genetic *** analyses were supplemented by flow cytometry of all the *** Findings All the material corresponds to hexaploid cytotype,indicating that ploidy variation does not play any role in the *** results indicate that temperature and precipitation were better predictors of genetic relatedness of the populations than geographic distance,suggesting that temperature and precipitation may be important determinants of population *** addition,precipitation,alone and in interaction with temperature,strongly affected population genotypic diversity suggesting increased clonality towards the coldest and especially the coldest wettest *** the same time,individuals from the coldest and wettest climates also had the high-est individual genetic diversity,suggesting that only the most hetero-zygous

关键词： AMOvA hexaploid isolation by distance isolation by environment microsatellites Poaceae fuzzy clustering

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Evidence of Electronic Phase Separation in the Strongly Correlated Semiconductor YbB_(12)

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Chinese Physics Letters 2022年第12期39卷 65-70页

作者： A.Azarevich N.Bolotina O.Khrykina A.Bogach E.zhukova B.Gorshunov A.Melentev Z.Bedran A.Alyabyeva M.Belyanchikov v.voronov N.Yu.Shitsevalova v.B.Filipov N.Sluchanko Prokhorov General Physics Institute of the Russian Academy of Sciences Vavilov Str.38Moscow 119991Russia Shubnikov Institute of Crystallography Federal Scientific Research Centre‘Crystallography and Photonics’of Russian Academy of Sciences59 Leninskiy ProspectMoscow 119333Russia Center for Photonics and 2D Materials Moscow Institute of Physics and Technology(National Research University)141700 DolgoprudnyMoscow RegionRussia Institute for Problems of Materials Science NASUKrzhizhanovsky Str.303142 KyivUkraine

We investigate high-quality single-domain crystals of YbB_(12)using the precise x-ray diffraction technique in combination with the low-temperature polarized THz–infrared spectroscopy and accurate magnetotransport *** is shown for the first time that this archetypal strongly correlated system with a metal-insulator transition to a mysterious dielectric ground state with a metal Fermi surface[Science 362,65(2018)and ibid 362,32(2018)]is actually a heterogeneous compound in the regime of electronic phase *** in the configuration of the discovered dynamic charge stripes are proposed upon *** a result,a conclusion is drawn in favor of a crossover between different patterns of the filamentary electronic structure penetrating the semiconducting matrix of YbB_(12).We argue that the discovery of stripes in YbB_(12)is fundamental,elucidating the nature of exotic dielectric state in Kondo insulators.

关键词： insulator Kondo dielectric

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Repellent effect of synanthropic house mouse urine odor on small forest mammals

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Current Zoology 2023年第3期69卷 315-323页

作者： Igor A.Zhigarev vasiliy v.Alpatov Dmitry A.Shitikov maria v.Nekrasova Olga G.Alekseeva Elena v.Kotenkova Department of Zoology and Ecology Institute of Biology and ChemistryMoscow Pedagogical State University129164 Kibalchicha Str.6/3MoscowRussia Laboratory for Behaviour and Behavioural Ecology of Mammals A.N.Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences119071 Leninsky prospect33MoscowRussia Department of Zoology Ecology and Nature ConservationK.I.Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology109472 Skrvabin Str.23MoscowRussia

In this study,we examined the effect of synanthropic house mouse(Mus musculus)urine odor on catching probability of small mammals to live *** conducted a series of field experiments in August 2016 and 2017 in a natural forests of the northwestern Moscow Region(Russia).Small mammals were trapped at two 4-ha fields using capture-mark-recapture technique by setting 200 live traps(100 points,2 traps per point)within each *** trap in each pair was odorless(control)with bait only,whereas the other one was odor-baited with 20μL of the urine of a synanthropic house *** analysis was based on the data collected from 2 rodent species(bank vole Myodes glareolus,herb field mouse Apodemus uralensis)and 3 shrew species(common shrew Sorex araneus,Laxmann’s shrew Sorex caecutiens,and Eurasian pygmy shrew Sorex minutus).As a result,only bank voles significantly avoided odor-baited live *** generalized linear mixed models,we showed that the choice of a trap by bank voles depended on their age,whereas the probability of repeated capture to a certain live trap was related to their prior *** discuss the possible role of components of synanthropic house mouse urine in the population management of exoanthropic small mammals.

关键词： field experiment Mus musculus olfactory cues repellent response to traps small mammals

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Unveiling the Nature of Polar-ring Galaxies from Deep Imaging

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Research in Astronomy and Astrophysics 2022年第11期22卷 34-50页

作者： Aleksandr v.Mosenkov vladimir P.Reshetnikov maria N.Skryabina Zacory Shakespear Department of Physics and Astronomy N283 ESCBrigham Young UniversityProvoUT 84602USA Central(Pulkovo)Astronomical Observatory Russian Academy of SciencesPulkovskoye Chaussee 65/1196140 St.PetersburgRussia St.Petersburg State University 7/9 Universitetskaya Nab.199034 St.PetersburgRussia

General structural properties and low surface brightness tidal features hold important clues to the formation of *** this paper,we study a sample of polar-ring galaxies(PRGs)based on optical imaging from the Sloan Digital Sky Survey Stripe 82 and other deep *** investigate the deepest images of candidates for PRGs to *** carry out photometric decomposition on the host galaxies and associated polar structures that allows us to derive the structural properties of both *** are able to detect very faint tidal structures around most PRGs in our *** several galaxies,we can directly observe the formation of the polar ring due to merging,which is manifested in debris of the victim galaxy and an arc-like polar structure made up of its *** a few cases,we can discern signs of tidal *** results obtained indicate that the gravitational interaction and merging of galaxies are the most plausible mechanisms for the formation of PRGs.

关键词： data analysis techniques:photometric galaxies:structure

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