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Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China

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Journal of Integrative Agriculture 2018年第12期17卷 2724-2733页

作者： TIAN Zhong-ling munawar maria Eda Marie Barsalote Pablo Castillo ZHENG Jing-wu Laboratory of Plant Nematology Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University Institute for Sustainable Agriculture(IAS) Spanish National Research Council (CSIC) Campus de Excelencia Internacional Agroalimentario Key Lab of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture

The rice root-knot nematode Meloidogyne graminicola is a severe pest of rice. In China, it was first reported from Hainan Province, and later from several other provinces. In the present study, a rice root-knot nematode population found from the rice cultivation areas of Zhejiang Province, China is characterized via molecular analysis using internal transcribed spacer(ITS) and cytochrome c oxidase subunit Ⅱ(coxⅡ)-16 S rRNA genes and scanning electron microscopy(SEM) observations of males and the second-stage juveniles. Morphometric data and molecular sequence comparisons for all M. graminicola populations occurring in China are also provided. The overall morphology of M. graminicola found in Zhejiang match well with the original description, though males have a slightly longer body and stylet, and a shorter tail, while the second-stage juvenile is also slightly longer than in the original description. This is the first report of M. graminicola from Zhejiang. Phylogenetic studies based on coxⅡ suggest that all the Chinese populations belong to Type B. This study expands knowledge of the increasing distribution and phylogenetic relationships of M. graminicola that occur in China.

关键词： Meloidogyne graminicola morphology morphometric molecular rice rDNA cytochrome c oxidase subunit Ⅱ (coxⅡ) China

Diagnosis and characterization of the ribosomal DNA-ITS of potato rot nematode(Ditylenchus destructor) populations from Chinese medicinal herbs

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Journal of Integrative Agriculture 2023年第6期22卷 1763-1781页

作者： NI Chun-hui HAN Bian LIU Yong-gang maria munawar LIU Shi-ming LI Wen-hao SHI Ming-ming LI Hui-xia PENG De-liang College of Plant Protection Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu ProvinceLanzhou 730070P.R.China Institute of Plant Protection Gansu Academy of Agricultural SciencesLanzhou 730070P.R.China Department of Biological Sciences University of LethbridgeLethbridge 4401Canada State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

The potato rot nematode(Ditylenchus destructor) is a very economically important nematode in agronomic and horticultural plants worldwide. In this study, 43 populations of D. destructor were collected from different hosts across China, including 37 populations from Chinese herbal medicine plants. Obtained sequences of ITS-rDNA and D2–D3of 28S-rDNA genes of D. destructor were compared and analyzed. Nine types of significant length variations in ITS sequences were observed among all populations. The differences in ITS1 length were mainly caused by the presence of repetitive elements with substantial base substitutions. Reconstructions of ITS1 secondary structures showed that the minisatellites formed a stem structure. Ten haplotypes were observed in all populations based on mutations and variations of helix H9. Among them, 3 known haplotypes(A–C) were found in 7 populations isolated from potato,sweet potato, and Codonopsis pilosula, and 7 unique haplotypes were found in other 36 populations collected from C. pilosula and Angelica sinensis compared with 7 haplotypes(A–G) according to Subbotin' system. These unique haplotypes were different from haplotypes A–G, and we named them as haplotypes H–N. The present results showed that a total of 14 haplotypes(A–N) of ITS-rDNA have been found in D. destructor. Phylogenetic analyses of ITSrDNA and D2–D3 showed that all populations of D. destructor were clustered into two major clades: one clade only containing haplotype A from sweet potato and the other containing haplotypes B–N from other plants. For further verification, PCR-ITS-RFLP profiles were conducted on 7 new haplotypes. Collectively, our study suggests that D. destructor populations on Chinese medicinal materials are very different from those on other hosts and this work provides a paradigm for relevant researches.

关键词： Ditylenchus destructor minisatellites ITS-RFLP phylogeny RNA secondary structure

Characterization and diagnostics of stubby root nematode Trichodorus cedarus Yokoo,1964(Dorylaimida:Trichodoridae)from Zhejiang Province,China

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Characterization and diagnostics of stubby root nematode Tri...

Ultrastructure,Morphological and Molecular Characterization of New and New Recoreded Species of Hemicriconemoides from China

中国植物病理学会2017年学术年会

作者： Barsalote Eda Marie munawar maria Tian Zhongling Cai Ruihang Li Xiaolin Qu Nan 郑经武 Laboratory of Plant Nematology Institute of BiotechnologyCollege of Agriculture &BiotechnologyZhejiang University

The study was conducted to characterize morphological and molecular profiles of seven isolates of Trichodorus cedarus recovered from deciduous trees and ornamentals in Zhejiang Province,Eastern *** of species was derived from polytomous key using prime character codes:E,G,H and subgroup codes:J,K,L,M for male populations while prime character codes:D,C,L,K and subgroup codes:G,H,J for female *** is characterized by a short body(0.63.90 mm),onchiostyle 559μm and secretory-excretory pore(S-E) situated along the anterior 2/3 of pharyngeal *** females,*** is distinguished by possessing didelphic genital system,transverse slit vulva located 52%60% along the body,vaginal refractive pieces approximately 1 1.9μm size,vagina pear-shaped withtriangular to rounded triangular vaginal *** males,*** is characterized by having 3 ventromedian cervical papillae(CP) located between onchiostyle base and S-E pore,presence of 3 ventromedian precloacal supplements with posterior supplement(SP 1)at level of retracted spicule,large sperms with sausage-shaped nucleus,spicule short(38.1 45.4 pμm)appearing slightly curved or nearly straight,manubrium offset without capitular extension and shaft more or less cylindrical with continuous fine ornamentations except at *** codes for identification of species are:D1-C1-L1-K2-A12-B23-E3-F1-G2-Hl-I2-J1-M1-N1-O1-P1-Q2-R1 in females and F34-D3-P2-A21-B23-C21-E0-G1-H3-I1-J21-K32-L2-M2-N1-O5 in *** based on partial 18 S,ITS1 and D2-D3 segments of 28 S obtained a fragment length of 1 726 743 bp,1 221 225 bp and 79699 bp,*** results revealed 99% sequence identity to *** ZJ8(HM106502).Similarly,phylogenetic reconstruction showed cluster of present isolates to *** putative species from Zhejiang with 99% clade *** paper takes into account the morphological and molecular comparisons among intraspecies of ***,the most prevalent tric

关键词： Stubby root nematode Morphology rDNA China

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Ultrastructure,Morphological and Molecular Characterization ...

Biodegradability of bioplastics in different aquatic environments:A systematic review

中国植物病理学会2017年学术年会

作者： maria munawar Tian Zhongling Eda Marie Barsalote Cai Ruihang Li Xiaolin Qu Nan 郑经武 Laboratory of Plant Nematology Institute of BiotechnologyCollege of Agriculture &BiotechnologyZhejiang University

Sheathoid nematodes of the genus Hemicriconemoides are migratory root-ectoparasites of many plants including various agricultural crops and fruit *** are generally found inhabiting tropical and subtropical areas of the world and have been reported from Africa,America,Asia,Australia and southern Europe,presently genus consists of 52 valid *** this study we provide morphological and molecular characterization of one new and one new recorded species *** *** *** isolated from Metasequoia glyptostroboides and Camelliae uruku in Hangzhou,Zhejiang,*** new species is most similar to *** and considered as a cryptic *** the new species can be characterized by cuticular sheath well separated at anterior region,cuticle smooth with retrorse annuli,lip region slightly set off and roughly square in ***733μm long,excretory pore 4 annuli posterior to pharyngeal bulb,vulva posteriorly located88%91%of the total body,tail conical with bluntly pointed *** can be characterized by its exclusive lip morphology(set off,marked with constriction),cuticular sheath attached at anterior and vulval region,stylet 668μm long,excretory pore located 4 5 annuli posterior to pharyngeal bulb,vulva posteriorly located 88%0%of the body,tail elongated widely conoid,ended up in a rounded *** electron microscopic observations and molecular characterization based on COI of mtDNA,18 S,D2-D3 28 S and ITS of rDNA sequences for both species also *** date sixteen Hemicriconemoides species have been reported from China,where *** is the 17 recorded *** results suggested that the concomitant use of morphological and molecular characterization help to remove the taxonomic opaqueness and paucity of knowledge regarding species *** will help to improve its relevance for posterity and will further elucidate the deeper levels of phylogenetic relationship

关键词： Hemicriconemoides Morphology Ultrastructure rDNA China

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Journal of Environmental Sciences 2024年第8期142卷 169-181页

作者： maria Cristina Lavagnolo Valentina Poli Anna maria Zampini Valentina Grossule Department of Civil Environmental and Architectural Engineering-Laboratory of Environmental EngineeringUniversity of PadovaLungargine Rovetta 8Padova 35100Italy

Bioplastics were first introduced as environmentally friendly materials,with properties similar to those of conventional plastics.A bioplastic is defined as biodegradable if it can be decomposed into carbon dioxide under aerobic degradation,or methane and CO_(2)under anaerobic conditions,inorganic compounds,and new cellular biomass,by the action of naturally occurring *** definition however does not provide any information on the environmental conditions,timescale and extent at which decomposition processes should *** regard to the aquatic environment,recognized standards have been established to assess the ability of plastics to undergo biodegradation;however,these standards fail to provide clear targets to be met to allow labelling of a bioplastic as ***,these standards grant the user an extensive leeway in the choice of process *** these reasons,the comparison of results deriving from different studies is *** authors analysed and discussed the degree of biodegradability of a series of biodegradable bioplastics in aquatic environments(both fresh and salt water)using the results obtained in the laboratory and from on-site testing in the context of different research *** Oxygen Demand(BOD),CO_(2)evolution,surface erosion and weight loss were the main parameters used by researchers to describe the percentage of *** results showed a large variability both in weight loss and BOD,even when evaluating the same type of *** confirms the need for a reference range of values to be established with regard to parameters applied in defining the biodegradability of bioplastics.

关键词： Biodegradation Bioplastic Plastic Natural environment Plastic waste

Carbon sequestration in a bamboo plantation:a case study in a Mediterranean area

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Journal of Forestry Research 2024年第3期35卷 231-238页

作者： Tommaso Chiti Emanuele Blasi maria Vincenza Chiriaco Department for Innovation in Biological Agro-Food and Forest Systems(DIBAF)University of TusciaVia San C.De Lellis snc01100 ViterboItaly Foundation Euro-Mediterranean Center on Climate Change(CMCC) Division on Climate Change Impacts on AgricultureForests and Ecosystem Services(IAFES)Via Garbini5101100 ViterboItaly

In the Mediterranean region,despite bamboo being an alien species that can seriously alter plant and ani-mal biocoenosis,the area occupied by bamboo plantations continues to increase,especially for the purpose to seques-ter carbon(C).However,the C dynamics in the soil-plant system when bamboo is grown outside its native area are poorly *** we investigated the C mitigation potential of the fast-growing Moso bamboo(Phyllostachys edulis)introduced in Italy for climate-change *** analyzed aboveground(AGB)and belowground(as root/shoot ratio)biomass,litter and soil organic C(SOC)at O-15-and 15-30-cm depths in a 4-year-old bamboo plantation in comparison with the former annual cropland on which the bamboo was *** have an idea of the maximum C stored at an ecosystem level,a natural forest adjacent the two sites was also *** the plantation,C accumulation as AGB was stimulated,with 14.8±3.1 Mg C ha^(-1) stored in 3 years;because thinning was done to remove culms from the first year,the mean sequestration rate was 4.9 Mg C ha^(-1) a^(-1).The sequestration rates were high but comparable to other fast-growing tree species in Italy(e.g.,Pinus nigra).SOC was significantly higher in the bamboo plantation than in the cropland only at the 0-15 cm depth,but SOC stock did not *** 4 years were not enough time for a clear increase in SOC,or the high nutrient uptake by bamboos might have depleted the soil nutrients,thus inhibiting the soil organic matter formation by *** comparison,the natural forest had significantly higher C levels in all the *** C dynamics at an ecosystem level,the bamboo plantation on the former annual cropland led to substantial C removal from the atmosphere(about 12 Mg C ha^(-1) a^(-1)).However,despite the promising C sequestration rates by bamboo,its introduction should be carefully considered due to potential ecological problems caused by this species in overexploited environments such as the Med

关键词： Carbon sequestration Climate change mitigation Mediterranean area Moso bamboo Soil organic carbon

Has the open surgical approach in colorectal cancer really become uncommon?

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World Journal of Gastrointestinal Surgery 2024年第6期16卷 1485-1492页

作者： maria Cariati Giuseppe Brisinda maria Michela Chiarello Department of Surgery Azienda Sanitaria Provinciale di CrotoneCrotone 88900Italy Abdominal and Endocrine Metabolic Medical and Surgical Sciences Fondazione Policlinico Universitario A Gemelli IRCCSRome 00168Italy Department of Surgery Azienda Sanitaria Provinciale di CosenzaCosenza 87100Italy

Colorectal cancer is the third most common cancer in the *** is man-datory to treat patients with colorectal *** colorectal cancer be treated in laparoscopy?Scientific literature has validated the oncological quality of laparo-scopic approach for the treatment of patients with colorectal *** non-inferiority trials with good remote control have answered positively to this long-debated *** as 1994,first publications demonstrated technical feasibility and compliance with oncological imperatives and,as far as short-term outcomes are concerned,there is no difference in terms of mortality and post-operative morbidity between open and minimally invasive surgical approaches,but only longer operating times at the beginning of the ***,from 2007 onwards,long-term results were published that demonstrated the ab-sence of a significant difference regarding overall survival,disease-free survival,quality of life,local and distant recurrence rates between open and minimally in-vasive *** this editorial,we aim to summarize the clinical and technical aspects which,even today,make the use of open surgery relevant and necessary in the treatment of patients with colorectal cancer.

关键词： Colorectal cancer Laparoscopy Laparoscopic colorectal resection Bowel obstruction Bowel perforation Advanced colorectal cancer

History of chronic gastritis:How our perceptions have changed

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World Journal of Gastroenterology 2024年第13期30卷 1851-1858页

作者： Dmitry Bordin maria Livzan Department of Pancreatic Biliary and Upper GI Tract DiseasesA.S.Loginov Moscow Clinical Scientific CenterMoscow 111123Russia Department of Propaedeutic of Internal Diseases and Gastroenterology Russian University of MedicineMoscow 127006Russia Department of Outpatient Therapy and Family Medicine Tver State Medical UniversityTver 170100Russia Department of Internal Medicine and Gastroenterology Omsk State Medical UniversityOmsk 644099Russia

Currently,the diagnostic strategy for chronic gastritis(CG)is aimed not just at fixing the presence of gastric mucosal inflammation,but also at gastric cancer(GC)risk stratification in a particular *** classification approach with the definition of the stage of gastritis determines the need,activities and frequency of dynamic monitoring of a ***,this attitude to the patient suffering from CG was far from *** present publication is a literature review describing the key milestones in the history of CG research,from the description of the first observations of inflammation of the gastric mucosa,assessment of gastritis as a predominantly functional disease,to the advent of endoscopy of the upper digestive tract and diagnostic gastric biopsy,assessment of the role of Helicobacter pylori infection in progression of inflammatory changes to atrophy,intestinal metaplasia,dysplasia and GC.

关键词： Chronic gastritis Intestinal metaplasia Dysplasia Gastric cancer Helicobacter pylori

Kinetic scale magnetic holes in the terrestrial magnetosheath:A review

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Science China Earth Sciences 2024年第9期67卷 2739-2771页

作者： Quanqi SHI Shutao YAO maria HAMRIN Ji LIU Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment Institute of Space SciencesShandong UniversityWeihai 264209China Department of Physics UmeåUniversityUmeå90746Sweden Department of Physics University of AlbertaEdmonton T6K 2P6Canada

Magnetic holes at the ion-to-electron kinetic scale(KSMHs)are one of the extremely small intermittent structures generated in turbulent magnetized *** recent years,the explorations of KSMHs have made substantial strides,driven by the ultra-high-precision observational data gathered from the Magnetospheric Multiscale(MMS)*** review paper summarizes the up-to-date characteristics of the KSMHs observed in Earth’s turbulent magnetosheath,as well as their potential impacts on space *** review starts by introducing the fundamental properties of the KSMHs,including observational features,particle behaviors,scales,geometries,and distributions in terrestrial *** have discovered that KSMHs display a quasi-circular electron vortex-like structure attributed to electron diamagnetic *** electrons exhibit noticeable non-gyrotropy and undergo *** occurrence rate of KSMH in the Earth’s magnetosheath is significantly greater than in the solar wind and magnetotail,suggesting the turbulent magnetosheath is a primary source ***,KSMHs have also been generated in turbulence simulations and successfully reproduced by the kinetic equilibrium ***,KSMHs have demonstrated their ability to accelerate electrons by a novel non-adiabatic electron acceleration mechanism,serve as an additional avenue for energy dissipation during magnetic reconnection,and generate diverse wave phenomena,including whistler waves,electrostatic solitary waves,and electron cyclotron waves in space *** results highlight the magnetic hole’s impact such as wave-particle interaction,energy cascade/dissipation,and particle acceleration/heating in space *** end this paper by summarizing these discoveries,discussing the generation mechanism,similar structures,and observations in the Earth’s magnetotail and solar wind,and presenting a future extension perspective in this active field.

关键词： Magnetic hole Magnetic dip/cavity Electron vortex Magnetosheath Turbulence Coherent structure Mirror mode Kinetic scale Electron acceleration Whistler