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International Journal of Ophthalmology(English edition) 2018年第5期11卷 802-806页

作者： Jordana Sandes Larissa R.S.Stival marcos pereira de avila Paulo Ferrara Guilherme Ferrara Leopoldo Magacho Luana P.N.Araujo Leonardo Torquetti Center of Reference in Ophthalmology (CEROF) Goiania 64605-020 Brazil Paulo Ferrara Eye Clinic Belo Horizonte-MG 30110-921 Brazil Fundacao Altino Ventura Recife-PE 50070-040 Brazil Center of Excellence in Ophthalmology Para de Minas35660-017 Brazil

AIM：To evaluate the clinical and tomographic outcomes after implantation of a new intrastromal corneal ring segment（ICRS） with 140-degrees of arc in eyes with corneal ***：We evaluated patients with corneal ectasia implanted with Ferrara 140° ICRS from April 2010 to February *** measures included preoperative and postoperative corrected distance visual acuity（CDVA）,keratometry simulated（K） reading,tomographic astigmatism and *** patients were evaluated using the Pentacam Scheimpflug ***：The study evaluated 58 *** mean followup was 16.81±10.8 *** CDVA（logM AR） improved from 0.5±0.20（20/60） to 0.3±0.21（20/40）（P〈0.01）.The average K reduced from 49.87±7.01 to 47.34±4.90 D（P〈0.01）.The asphericity changed from-0.60±0.86 to-0.23±0.67 D（P〈0.01）.The mean preoperative tomographic astigmatism decreased from-8.0±3.45 to-4.53±2.52 D（P〈0.01）.CONCLUSION：The new ICRS model with 140-degrees of arc effectively improve the visual acuity and reduce the high astigmatism usually found in patients with corneal ectasia.

关键词： keratoconus intrastromal corneal ring segment corneal ectasia

Implant surface features as key role on cell behavior

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BIOCELL 2022年第5期46卷 1151-1156页

作者： RAFAEL SCAF de MOLON MARTA MARIA ALVES pereira ERICA DORIGATTI de avila Federal University of Rio de Janeiro Rio de Janeiro21941-617Brazil São Paulo State University Araraquara14801-930Brazil

It has been recognized that physical and chemical properties of biomaterial surfaces mediate the quality of extracellular matrix(ECM)that may affect cell *** nature,ECM is a heterogeneous three-dimensional superstructure formed by three major components,glycosaminoglycan,glycoconjugate,and protein,that anchors cellular compartments in tissues and regulates the function and the behavior of *** in the biointerface alter the quality of ECM and morphology through cell surface receptors,which,in turn,enable it to trigger specific cell signaling and different cellular *** fact,a number of strategies have been used to improve the functionality of surfaces and direct cell behavior through precisely designed ***,we aimed to discuss,through a science-based viewpoint,the biomaterial surface features on cell behavior and analyze the impact of cell physical modification on dental implant development.

关键词： Biomaterial Cell adhesion Surface chemistry Surface topography

Long-term forest management after wildfire(Catalonia,NE Iberian Peninsula)

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Journal of Forestry Research 2020年第1期31卷 269-278页

作者： marcos Francos Xavier Ubeda Paulo pereira GRAM(Grup de Recerca Ambiental Mediterrania) Department of GeographyUniversity of BarcelonaMontalegre608001 BarcelonaSpain Environmental Management Centre Mykolas Romeris UniversityVilniusLithuania

Studies of post-fire soil status in Mediterranean ecosystems are common;however,few have examined the effects of long-term forest management after a wildfire on physicochemical soil ***,we analyzed differences in soil properties attributable to long-term postfire management and assessed the sustainability of these management practices in relation to the soil *** study area is located in Odena in the northeast region of the Iberian Peninsula consisted of the control forest(burned more than 30 years ago),low density forest(LD;burned in a wildfire in 1986 and managed in 2005)and high density forest(HD;burned in a wildfire in 1986 and no managed).For soils from each plot,we measured soil water repellency,aggregate stability,total nitrogen(TN),soil organic matter(SOM),inorganic carbon(IC),pH,electrical conductivity,extractable calcium,magnesium,sodium,potassium(K),phosphorus,aluminum(Al),manganese(Mn),iron(Fe),zinc,copper,boron,chrome,silicon and sulfur and calculated the ratios of C/N,Ca+Mg/(Na+K)^1/2,Ca/Al and Ca/*** differences were found in TN,IC,SOM,pH,K,Al,Mn,Fe and C/N ratio(pdegradation in areas affected by wildfire in the medium-and long-term by improving soil properties.

关键词： Soil chemical properties Aggregate stability Post-fire management Wildfire risk Vegetal density

Effect of pre-and post-wildfire management practices on plant recovery after a wildfire in Northeast Iberian Peninsula

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Journal of Forestry Research 2020年第5期31卷 1647-1661页

作者： marcos Francos Paulo pereira Xavier Ubeda GRAM(Grup de Recerca Ambiental Mediterrania) Department of GeographyUniversity of BarcelonaMontalegre608001 BarcelonaSpain Environmental Management Centre Mykolas Romeris UniversityVilniusLithuania

Fire and pre-or post-fire management practices shape the distribution and richness of plant ***,the effects of pre-and post-fire management on vegetation recovery were studied at different times,up to 18 months after a *** months after a 2015 wildfire,18 study plots were established(three 4-m^2 plots for each treatment),vegetation regrowth was monitored and vegetal species richness(S),evenness(IT),density(D),diversity(H0)and maximum diversity(HMax)after 2,10 and 18 *** treatments were(1)control,unaffected by 2015 wildfire;(2)no treatment(NT),burned in 2015 wildfire and not managed;(3)managed in 2005 and burned in 2015(M05B);(4)managed in 2015,2 months before wildfire(M15B);(5)cut and manual removal after the 2015 wildfire(CR);(6)cut and no trunk removal randomly deposited on topsoil after the 2015 wildfire(CL).All the treatments were carried out in a Pinus halepensis Miller *** 10 and 18 months after the wildfire,vegetation recovery was greater in NT,CR and CL plots than in M05B and M15B the *** 18 months after the wildfire,Brachypodium retusum(Pers.)*** Rosmarinus officinalis *** still dominant,especially in M15B,corroborating the belief that pre-fire treatment reduced ecosystem resilience and vegetal recovery compared to the NT and post-fire managed *** was significantly lower 10 months after wildfire in control plots,and IT was significantly higher in that inventory than previously in *** months after the wildfire,H0 was significantly lower in *** months post-wildfire,HMax was significantly lower in the control *** months after the wildfire,HMax,was significantly higher in CR,CL and M05B than in the control and M15B ***,pre-fire management was detrimental to post-fire vegetation recovery,while manual post-fire management proved beneficial.

关键词： Forest management Vegetation recovery Diversity Richness Evenness

Behavior of wood basic density according to environmental variables

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Journal of Forestry Research 2022年第2期33卷 497-505页

作者： Gabriel marcos Vieira Oliveira JoséMárcio de Mello Carlos Rogério de Mello JoséRoberto Soares Scolforo Eder pereira Miguel Thiago Campos Monteiro Brazil Institute of Agricultural and Forestry defense of Espírito Santo(IDAF) Av.Lourival Lugon Moulinnº300CentroJerônimo MonteiroES CEP:29.550-000Brazil department of Forestry Sciences Universidade Federal de Lavras-UFLACaixa Postal 3037LavrasMG CEP:37.200-000Brazil Engineering department Universidade Federal de Lavras-UFLACaixa Postal 3037LavrasMG CEP:37.200-000Brazil Forestry Engineering department Faculdade de TecnologiaUniversity of Brasilia-UnBCampus Universitário Darcy RibeiroCaixa Postal 4357Asa Norte-BrasíliaDF CEP:70910-900Brazil department of Forestry Engineering and Technology Universidade Federal do Paraná-UFPRAv.Pref.Lothário Meissner632Jardim BotânicoCuritibaPR CEP:80.210-170Brazil

The relationships between climate conditions and wood density in tropical forests are still poorly *** quantify spatial dependence of wood density in the state of Minas Gerais(MG,Brazil),map spatial distribution of density,and correlate density with climate variables,we extracted data from the Forest Inventory of Minas Gerais for 1988 trees scaled throughout the territory and measured wood density of discs removed from the *** variables were extracted from the database of the Ecological-Economic Zoning of Minas *** spatial analysis,tree densities were measured at 44 georeferenced sampling *** data were subjected to exploratory analysis,variography,cross-validation,model selection,and ordinary *** relationships between wood density and environmental variables were calculated using dispersion matrices,linear correlation,and *** density proved to be highly spatially dependent,reaching a correlation of 96%,and was highly continuous over a distance of 228 *** distribution of wood density followed a continuous gradient of 514-659 kg m^(−3),enabling corre-lation with environment *** was correlated with mean annual precipitation(−0.57),temperature(0.63),and evapotranspiration(0.83).Geostatistical methods proved useful in predicting wood density in native tropical forests with different climate *** results confirmed the sensitivity of wood density to climate change,which could affect future carbon stock in forests.

关键词： Biomass Climate variables Geostatistics Hardwood Forest inventory Minas Gerais

Inhibition of vertebrate complement system by hematophagous arthropods: inhibitory molecules, mechanisms, physiological roles, and applications

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Insect Science 2024年第5期31卷 1334-1352页

作者： Mauricio Roberto Vianna Sant'Anna Adalberto Alves pereira-Filho Antonio Ferreira Mendes-Sousa Naylene Carvalho Sales Silva Nelder Figueiredo Gontjo marcos Horacio pereira Leonardo Barbosa Koerich Grasielle Caldas D'avila Pessoa John Andersen Ricardo Nascimento Araujo department of Parasitology Institute of Biological SciencesUniversidade Federal de Minas GeraisBelo HorizonteMGBrazil Instituto Nacional de Ciencia e Tecnologia em Entomologia Molecular Rio de JaneiroRBrazil Campus Senador Helvidio Nunes de Barros Universidade Federal do PiauiPicosPlBrazil Vector Molecular Biology Section Laboratory of Malaria and Vector ResearchNational Institute of Allergy and Infectious DiseasesNational Institutes of HealthRockvilleMarylandUSA

In arthropods,hematophagy has arisen several times throughout *** specialized feeding behavior offered a highly nutritious diet obtained during blood *** the other hand,blood-sucking arthropods must overcome problems brought on by blood intake and *** blood complement acts on the bite site and is still ac-tive after ingestion,so complement activation is a potential threat to the host's skin feed-ing environment and to the arthropod gut *** evolution,blood-sucking arthropods have selected,either in their saliva or gut,anticomplement molecules that inac-tivate host blood *** review presents an overview of the complement sys-tem and discusses the arthropod's salivary and gut anticomplement molecules studied to date,exploring their mechanism of action and other aspects related to the arthropod-host-pathogen *** possible therapeutic applications of arthropod's anticomplement molecules arealsodiscussed.

关键词： alternative pathway classical pathway complement inhibitors hematophagy intestine salivary gland

Multiomic investigation of Sugarcane mosaic virus resistance in sugarcane

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The Crop Journal 2023年第6期11卷 1805-1815页

作者： Ricardo JoséGonzaga Pimenta Alexandre Hild Aono Roberto Carlos Villavicencio Burbano Marcel Fernando da Silva Ivan Antônio dos Anjos marcos Guimarães de Andrade Landell marcos Cesar Gonçalves Luciana Rossini Pinto Anete pereira de Souza Centre for Molecular Biology and Genetic Engineering University of CampinasCampinasBrazil Gustavo Galindo Velasco Campus Littoral Polytechnic Superior SchoolGuayaquilEcuador Advanced Centre for Technological Research in Sugarcane Agribusiness Agronomic Institute of CampinasRibeirão PretoBrazil Plant Protection Research Centre Biological InstituteSão PauloBrazil department of Plant Biology Institute of BiologyUniversity of CampinasCampinasBrazil

Sugarcane mosaic virus(SCMV)is the main etiological agent of sugarcane mosaic disease,which affects sugarcane and other grass *** the extensive characterization of quantitative trait loci controlling resistance to SCMV in maize,the genetic basis of this trait in sugarcane is largely ***,a genome-wide association study was performed and machine learning coupled with feature selection was used for genomic prediction of resistance to SCMV in a diverse sugarcane *** single-nucleotide polymorphisms(SNPs)explained up to 29.9%of the observed phenotypic variance and a 73-SNP set predicted resistance with high accuracy,precision,recall,and F1 scores(the harmonic mean of precision and recall).Both marker sets were validated in additional sugarcane genotypes,in which the SNPs explained up to 23.6%of the phenotypic variation and predicted resistance with a maximum accuracy of 69.1%.Synteny analyses suggested that the gene responsible for the majority of SCMV resistance in maize is absent in sugarcane,explaining why this major resistance source has not been identified in this ***,using sugarcane RNA-Seq data,markers associated with resistance to SCMV were annotated,and a gene coexpression network was constructed to identify the predicted biological processes involved in *** network allowed the identification of candidate resistance genes and confirmed the involvement of stress responses,photosynthesis,and the regulation of transcription and translation in resistance to *** results provide a practical marker-assisted breeding approach for sugarcane and identify target genes for future studies of SCMV resistance.

关键词： Saccharum SCMV GWAS Machine learning RNA-Seq

Pramipexole, a dopamine D3/D2 receptor-preferring agonist, attenuates reserpine-induced fibromyalgia-like model in mice

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Neural Regeneration Research 2022年第2期17卷 450-458页

作者： Carlos pereira Martins Rodrigo Sebben Paes Gabriela Mantovani Baldasso Eduarda Gomes Ferrarini Rahisa Scussel Rubya pereira Zaccaron Ricardo Andrez Machado-de-avila Paulo Cesar Lock Silveira Rafael Cypriano Dutra Laboratory of Autoimmunity and Immunopharmacology Department of Health SciencesCampus AraranguaUniversidade Federal de Santa CatarinaAraranguáSCBrazil Post-Graduate Program of Neuroscience Center of Biological SciencesUniversidade Federal de Santa CatarinaFlorianópolisSCBrazil Laboratory of Experimental Physiopathology Program of Postgraduate in Science of HealthUniversidade do Extremo Sul CatarinenseCriciumaSCBrazil

Fibromyalgia(FM) is a complex pathology described as persistent hyperalgesia including somatic and mood dysfunctions, depression and anxiety. Although the etiology of FM is still unknown, a significant decrease in biogenic amines is a common characteristic in its pathogenesis. Here, our main objective was to investigate the role of dopamine D3/D2 receptor during the reserpine-induced pain in mice. Our results showed that pramipexole(PPX) – a dopaminergic D3/D2 receptor agonist – inhibited mechanical allodynia and thermal sensitivity induced by reserpine. Relevantly, PPX treatment decreased immobility time and increased the number of grooming in the forced swimming test and splash test, respectively. Animals that received PPX remained longer in the open arms than the reserpine group using elevated plusmaze apparatus. The repeated PPX administration, given daily for 4 days, significantly blocked the mechanical and thermal allodynia during FM model, similarly to pregabalin, although it failed to affect the reserpine-induced thermal nociception. Reserpine administration induced significant downregulation of dopamine concentration in the central nervous system, and repeated treatment with PPX restored dopamine levels in the frontal cortex and spinal cord tissues. Moreover, PPX treatment inhibited oxidants production such as DCFH(2′,7′-dichlorodihydrofluorescein) and nitrite, also decreased oxidative damage(carbonyl), and upregulated the activity of superoxide dismutase in the spinal cord. Together, our findings demonstrated the ability of dopamine D3/D2 receptor-preferring agonist in reducing pain and mood dysfunction allied to FM in mice. All experimental protocols were approved by the Universidade Federal de Santa Catarina(UFSC) Ethics Committee(approval No. 2572210218) on May 10, 2018.

关键词： dopamine dopaminergic system fibromyalgia hyperalgesia pain pramipexole reserpine

Structure and genetic diversity of natural populations of Guadua weberbaueri in the southwestern Amazon,Brazil

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Journal of Forestry Research 2021年第2期32卷 755-763页

作者： Glória da Silva Almeida Leal Fabrício Assis Leal Hugo Teixeira Gomes Anderson marcos de Souza Sabina Cerruto Ribeiro Jonny Everson Scherwinski-pereira Centro Multidisciplinar Universidade Federal do AcreCruzeiro do SulAcreBrazil Universidade de Brasília BrasíliaDistrito FederalBrazil Centro de Ciências Biológicas e da Natureza Universidade Federal do AcreRio BrancoAcreBrazil Centro de Pesquisa e Aplicação de Bambu e Fibras Naturais Universidade de Brasília–CPAB/UnBBrasíliaDistrito FederalBrazil Embrapa Recursos Genéticos e Biotecnologia Av.W5 Norte(final)PqEBBrasíliaDistrito Federal 70770-917Brazil

The Brazilian state of Acre has an extensive natural reserve of bamboo,making it one of the largest in loco gene *** aim of this study was to characterize the structure and genetic diversity of Guadua weberbaueri *** two populations,one native(FAPB)and the other anthropized(FAPBA),using ISSR *** results show that the FAPB population exhibited higher values for all estimates of population ***,the FAPBA population also showed high heterozygosity,corroborated by estimated gene flow(Nm=3.9)between the *** study of the association between Nei’s genetic distances and the geographic distances between the populations were significantly correlated(r=0.45,p=0.01),corroborated by the dendrogram revealing two distinct groups corresponding to the collection sites,without mixing classes between populations in the same *** for the coancestry coefficient,pairs of individuals in the first distance class were positive and significant,indicating that plants that are geographically closer share common alleles with a frequency greater than by chance,which means that there is a tendency that geographically closer individuals are *** presented similar genetic structure when the geographical distance between them was up to 56 m for FAPB and up to 156 m for *** was concluded that anthropized environments exhibit less genetic diversity than native environments,inferring risks for species conservation if appropriate and planned management techniques are not adopted.

关键词： Guadua Bamboo Genetic diversity Amazon rainforest Anthropized and native populations Underutilization plants

Are models of volumetric production at the diametric distribution and total stand level mutually compatible?

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Journal of Forestry Research 2020年第5期31卷 1691-1698页

作者： marcos Felipe de Oliveira Valeriano Eder pereira Miguel Pedro Guilherme de Andrade Vasconcelos Mauro Eloi Nappo Humberto Angelo Alba Valeria Rezende Renan Augusto Miranda Matias Leonardo Job Biali Ilvan Medeiros Lustosa Junior department of Forestry Engineering University of Brasilia(UnB)Brası´liaBrazil Federal Institute of Brasılia(IFB) BrasıliaBrazil

The objective of this work was to compare estimates generated by a diametric distribution model and a total stand model against the pre-cut *** model efficiency was also *** were evaluated from 30 permanent sample plots in a Eucalyptus urophylla stand,comprising 24 sample plots used for model fitting,and six sample plots for *** volume of wood per hectare was estimated for different productive units(sites),using 7 years as the reference *** model adjustment quality was verified by adjustment and precision statistics:the correlation between observed and predicted variables,root mean square error percentage,graphical analysis of residual distribution,and a frequency histogram for classes of relative errors and *** the two-parameter Weibull probability density function adhered to the data for tree evolution in diameter classes for the reference age(7 years)in the different productivity classes,it generated imprecise estimates of the number of ***,it produced inaccurate volumetric production *** total stand model provided reliable projections of production volumes in different productivity classes for both adjustment types,showing compatibility with the pre-cut inventory according to a Tukey *** summary,the total stand model generated estimates that were compatible with the pre-cut inventory while the diametric distribution model did not.

关键词： Clutter model Diameter class Eucalyptus Prognosis