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Asterohyptis stellulata: Phytochemistry and wound healing activity

Food Bioscience 2022年第6期50卷 2067-2076页

作者： Nallely Álvarez-Santos Edgar Antonio Estrella-Parra José del Carmen Benítez-Flores Rocío Serrano-Parrales Tomás Ernesto Villamar-Duque martha Angelica Santiago-Santiago mar.a del Rosario González-Valle José Guillermo Avila-Acevedo Ana mar.a García-Bores Phytochemistry Laboratory UBIPROSuperior Studies Faculty (FES)-IztacalaNational Autonomous University of Mexico (UNAM)Tlalnepantla de BazMexico State54090Mexico Postgraduate Biological Sciences Postgraduate Studies UnitNational Autonomous University of Mexico (UNAM)CoyoacanMexico City04510Mexico Histology Laboratory 1 UMFSuperior Studies Faculty (FES)-IztacalaNational Autonomous University of Mexico (UNAM)Tlalnepantla de BazMexico State54090Mexico Natural Products Bioactivity Laboratory UBIPROSuperior Studies Faculty (FES)-IztacalaNational Autonomous University of Mexico (UNAM)Tlalnepantla de BazMexico State54090Mexico Bioterium Superior Studies Faculty (FES)-IztacalaNational Autonomous University of Mexico (UNAM)Tlalnepantla de BazMexico State54090Mexico Plant Physiology Laboratory UBIPROFES-IztacalaUNAMTlalnepantla de BazMexico State54090Mexico

Wound healing is a fast and efficient process. However, several factors can inhibit healing, causing scars and chronic wounds. This affects patients in their physical integrity, economic and emotional stability. Many plants that are used in wound healing treatment have antioxidant/anti-inflammatory activity through ROS regulation and antibacterial activity. Asterohyptis stellulata is used for wound treatment. Nonetheless, this activity has not been evaluated and its secondary metabolites are unknown. Therefore, the objective of this study was to analyze its phytochemical composition and evaluate the healing activity of A. stellulata. For the identification of secondary metabolites, the following were carried out: phytochemical screening, quantification of total phenols, and HPLC/MS analysis. The antibacterial and antioxidant activities were also determined. Next, to evaluate the healing activity of topical application of A. stellulata, the extract was mixed with the Beeler base to form an emulsion and an irritability test was performed. The emulsion was evaluated on incisional wounds of CD1 et/et mice, with tests of tensile strength, total re-epithelialization time, wound closure speed, and histological analysis. The phytochemical analysis showed that A. stellulata has phenolic compounds, two quercetin derivatives and a glycoside rosmarinic acid as main compounds. The extract exhibited medium antibacterial and antioxidant activities. A. stellulata emulsion was non-irritating, promoted closure speed of wound (1.479 ± 0.043 mm/day), increased tensile strength (980 ± 89.44 g), and histological analysis showed it promoted mice skin regeneration, compared to the controls (Recoveron® and Beeler base). This study confirms the traditional use of the A. stellulata.

关键词： Asterohyptis stellulata Phytochemistry Skin regeneration Wound healing

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Dixy： A Connection Between the “Typographic Appearance” and the Brain of Children

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Journal of Literature and Art Studies 2014年第1期4卷 55-61页

作者： mar.a Fernanda del Real García Universidad Autónoma de Barcelona Bellaterra Spain

This study presents a comparison between three different fonts in order to know the possible influence they may have when used in educational materials for children with readers difficulties resulting from dyslexia. Two of the three selected fonts （Arial and MeMimas） are frequently used in textbooks in Spain. The third type of letter （Dixy） was specially developed for typographic research purposes, following graphic features that according to literature and previous studies on reading patterns favor the dyslexic, and could improve reading skills in these individuals. To examine the quality of reading （speed and accuracy） between fonts, a small--but significant--sample was used. Ten children in Madrid, from eight to 10 years old were examined while reading words and pseudowords with three different fonts （Arial, MeMimas, and Dixy）. The result of the study shows the influence of the shapes of the letters in the legibility of texts with familiar and unfamiliar words （pseudowords） in children with dyslexia. The study showed that using the font Dixy, despite not being known by the children, reading is more accurate than using fonts known to them, such as Arial and MeMimas. As to the reading speed, the results indicate that, although the Dixy is a font never seen before by the children, reading speed is similar to a known font for them, as is the Arial, and greater than a hand writing font such as MeMimas.

关键词： legibility letter font dyslexia reading children readers difficulties

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Involvement of parathyroid hormone-related peptide in the aggressive phenotype of colorectal cancer cells

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World Journal of Gastroenterology 2021年第41期27卷 7025-7040页

作者： mar.a Belén Novoa Díaz Pedro Matías Carriere mar.a Julia martín Natalia Calvo Claudia Gentili Departamento de Biología Bioquímica y FarmaciaUniversidad Nacional del Sur(UNS)-INBIOSUR(CONICET-UNS)Bahía Blanca 8000Buenos AiresArgentina Departamento de Química Universidad Nacional del Sur(UNS)-INQUISUR(CONICET-UNS)Bahía Blanca 8000Buenos AiresArgentina

Colorectal cancer(CRC)remains one of the leading causes of mortality from malignant diseases *** general terms,CRC presents high heterogeneity due to the influence of different genetic and environmental factors;also,the neoplastic cells are strongly influenced by the extracellular matrix and several surrounding cells,known together as the tumor microenvironment(TME).Bidirectional communication takes place between the tumor and the TME through the release of autocrine and paracrine *** hormone-related peptide(PTHrP)is a cytokine secreted by a wide variety of tissues and is able to regulate several cellular functions both in physiological as well as in pathological *** exerts its effects as a paracrine/autocrine factor,although its mode of action is mainly *** has been shown that this peptide is expressed by several tumors and that the tumor secretion of PTHrP is responsible for the malignant humoral *** years ago,when our research group started studying PTHrP effects in the experimental models derived from intestinal tumors,the literature available at the time addressing the effects of PTHrP on colorectal tumors was limited,and no articles had been published regarding to the paracrine action of PTHrP in CRC *** on this and on our previous findings regarding the role of PTH in CRC cells,our purpose in recent years has been to explore the role of PTHrP in *** analyzed the behavior of CRC cells treated with exogenous PTHrP,focalizing in the study of the following events:Survival,cell cycle progression and proliferation,migration,chemoresistance,tumor-associated angiogenesis,epithelial to mesenchymal transition program and other events also associated with invasion,such us the induction of cancer stem cells *** work summarizes the major findings obtained by our investigation group using in vitro and in vivo CRC models that evidence the participation of PTHrP in the acquisition of an aggressiv

关键词： Parathyroid hormone-related protein Colorectal cancer Tumor biomarkers Neoplastic processes Drug resistance Tumor microenvironment

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Refractive outcomes after V4c Toric collamer lens implantation over 1y of follow-up

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International Journal of Ophthalmology(English edition) 2024年第7期17卷 1322-1330页

作者： Humberto Carreras-Díaz Josefina Reñones de Abajo mar.a del Rosario Carreras-Díaz Amalia Lorente-Velázquez Vithas Eurocanarias Las Palmas de Gran Canaria 35004Spain University Hospital of Girona Doctor Josep Trueta Gerona 17007Spain Departament of Optometry and Vision Faculty of Optics and OptometryComplutense University of MadridMadrid 28040Spain

AIM:To evaluate refractive outcomes and corneal astigmatism changes after Toric implantable collamer lens with a central port(V4c T-ICL)implantation over 1y of ***:A retrospective study was performed including 50 eyes of 50 patients that underwent V4c T-ICL ***(UDVA)and corrected(CDVA)distance visual acuities,refraction,refractive and corneal astigmatism changes and corneal coupling correction were evaluated preoperatively,1 and 12mo *** analysis was used for astigmatism *** of adjustment(CAdj)was calculated for corneal coupling ***:The mean UDVA achieved was 0.03 logmar.at 1mo and remained unchanged throughout the whole follow-up(P=0.193).At the last visit,84%of the eyes achieved a CDVA of 0.00 logmar or *** spherical equivalent refraction(SEQ),96%of eyes were ranges of±1.00 D and 84%of them within±0.50 ***,94%of eyes had a remaining refractive cylinder within±1.00 D and 78%of them within±0.50 ***,SEQ and refractive cylinder,remain stable over the postoperative follow-up(P=1.000 and P=0.660,respectively).In terms of surgically induced astigmatism(SIA),no statistically significant differences were found over the follow-up(P=0.102)and under correction was found with a correction index lower than the unit at each visit.A keratometric astigmatism induced of 0.59±0.53(vector mean:0.26×73º)D was reached at the last *** significant changes in terms of corneal astigmatism orientation were reported over post-surgery visits(P=0.129 and P=0.097 at 1 and 12mo respectively).No clinical significance was found for CAdj on with-the-rule *** postoperative complications resulting from the surgery were ***:Refractive outcomes suggest that the V4c T-ICL implantation for correction of myopic astigmatism was satisfactory in terms of effectiveness,safety,and stability during 1y of *** astigmatism induced by the incision around 0.5 D is achieved

关键词： V4c Toric collamer lens corneal astigmatism surgically induced astigmatism corneal coupling

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Insights into the structural biology of G-protein coupled receptors impacts drug design for central nervous system neurodegenerative processes

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Neural Regeneration Research 2013年第24期8卷 2290-2302页

作者： Farfán-García Eunice Dalet Trujillo-Ferrara José Guadalupe Castillo-Hernández mar.a del Carmen Guerra-Araiza Christian Humberto Soriano-Ursúa marvin Antonio Escuela Superior de Medicina Instituto Politécnico Nacional. Plan de San Luis y Díaz Mirón s/n Col. Casco de Santo Tomas

In the last few years, there have been important new insights into the structural biology of G-protein coupled receptors. It is now known that allosteric binding sites are involved in the affinity and selec- tivity of ligands for G-protein coupled receptors, and that signaling by these receptors involves both G-protein dependent and independent pathways. The present review outlines the physiological and pharmacological implications of this perspective for the design of new drugs to treat disorders of the central nervous system. Specifically, new possibilities are explored in relation to allosteric and or- thosteric binding sites on dopamine receptors for the treatment of Parkinson＇s disease, and on muscarinic receptors for Alzheimer＇s disease. Future research can seek to identify ligands that can bind to more than one site on the same receptor, or simultaneously bind to two receptors and form a dimer. For example, the design of bivalent drugs that can reach homo/hetero-dimers of D2 dopa- mine receptor holds promise as a relevant therapeutic strategy for Parkinson＇s disease. Regarding the treatment of Alzheimer＇s disease, the design of dualsteric ligands for mono-oligomeric mus- carinic receptors could increase therapeutic effectiveness by generating potent compounds that could activate more than one signaling pathway.

关键词： neural regeneration G-Protein coupled receptors structural biology drug design neurodegenera-tive disorders , oligomedzation biased signaling Parkinson's disease Alzheimer's disease dopa-mine receptors muscarinic receptors grants-supported paper neuroregeneration

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Experimental bio-artificial liver:Importance of the architectural design on ammonia detoxification performance

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World Journal of Hepatology 2018年第10期10卷 719-730页

作者： mar.a Dolores Pizarro mar.a Eugenia Mamprin Lucas Damián Daurelio Joaquín Valentín Rodriguez mar.a Gabriela Mediavilla Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Rosario S2002 LRKArgentina Laboratorio de Investigaciones en Fisiología y Biología Molecular Vegetal(LIFiBVe) Facultad de Ciencias AgrariasUniversidad Nacional del LitoralEsperanza 3080Argentina Facultad de Ciencias Bioquímicas y Farmacéuticas Universidad Nacional de RosarioRosario S2002 LRKArgentina Centro Binacional de Criobiología Clínica y Aplicada(CAIC) Universidad Nacional de RosarioRosario S2011 BXNArgentina Instituto de Biología Molecular y Celular de Rosario(IBR CONICET-UNR)Consejo Nacional de Investigaciones Científicas y Tecnológicasy Universidad Nacional de RosarioRosario S2002 LRKArgentina

AIM To determine the influence of the construction design over the biological component's performance in an experimental bio-artificial liver(BAL) *** Two BAL models for liver microorgans(LMOs) were constructed. First, we constructed a cylindrical BAL and tested it without the biological component to establish its correct functioning. Samples of blood and biological compartment(BC) fluid were taken after 0, 60, and 120 min of perfusion. Osmolality, hematocrit, ammonia and glucose concentrations, lactate dehydrogenase(LDH) release(as a LMO viability parameter), and oxygen consumption and ammonia metabolizing capacity(as LMO functionality parameters) were determined. CPSI and OTC gene expression and function were measured. The second BAL, a "flat bottom" model, was constructed using a 25 cm2 culture flask while maintaining all other components between the models. The BC of both BALs had the same capacity(approximately 50 cm3) and both were manipulated with the same perfusion system. The performances of the two BALs were compared to show the influence of *** The cylindrical BAL showed a good exchange of fluids and metabolites between blood and the BC, reflected by the matching of osmolalities, and glucose and ammonia concentration ratios after 120 min of perfusion. No hemoconcentration was detected, the hematocrit levels remained stable during the whole study, and the minimal percentage of hemolysis(0.65% ± 0.10%) observed was due to the action of the peristaltic pump. When LMOs were used as biological component of this BAL they showed similar values to the ones obtained in a Normothermic Reoxygenation System(NRS) for almost all the parameters assayed. After 120 min, the results obtained were: LDH release(%): 14.7 ± 3.1 in the BAL and 15.5 ± 3.2 in the NRS(n = 6); oxygen consumption(μmol/min?g wet tissue): 1.16 ± 0.21 in the BAL and 0.84 ± 0.15 in the NRS(n = 6); relative expression of Cps1 and Otc: 0.63 ± 0.12 and 0.67 ± 0.20, respectively,

关键词： Bio-artificial liver Ammonia detoxification Device design Ornithine Transcarbamylase Rat liver microorgans Carbamyl Phosphate SynthetaseⅠ

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Land cover changes and fragmentation in mountain neotropical ecosystems of Oaxaca, Mexico under community forest management

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Journal of Forestry Research 2019年第1期30卷 143-155页

作者： Rafael M~a Navarro Cerrillo Dennis J.Esteves Vieira Susana Ochoa-Gaona Bernardus H.J.de Jong M~a del mar delgado Serrano Depto. Ingeniería Forestal Laboratorio de SelviculturaDendrocronología y Cambio Climático.Dendrodat Lab-ERSAF Universidad de Córdoba Depto. de Engenharia Florestal Faculdade de Engenharia Florestal Laboratório de Sensoriamento Remoto e Geotecnologia Universidade Federal de Mato Grosso Departamento de Ciencias de la Sustentabilidad El Colegio de la Frontera Sur-Unidad Campeche Depto. Economía Sociología y Política Agrarias Grupo de Evaluación y Restauración de Sistemas Agrícolas y Forestales-ERSAF Universidad de Córdoba

Changes in land cover have a direct impact on forest ecosystem goods and services. In this study, changes in land cover in Sierra de Juarez–Oaxaca ecosystems were estimated using a consistent processing of Landsat images and OBIA methodology. Additionally, landscape analyses using FRAGSTAT were conducted. In 2014, Sierra de Juarez–Oaxaca was covered by approximately 84% of forests, mainly pine-oak and cloud forests. After extensive deforestation until 2001, this trend was reversed and the forest cover surface area in 2014 was slightly higher than in 1979. The comparison of the landscape structure of the forested and agricultural lands suggests an increase in habitat heterogeneity. However, interspersion and juxtaposition indices, showing the patch shape by patch area and perimeter, were similar throughout the study period(1979–2014). Social and economic drivers can explain this situation: namely, community organization, forest enterprises, payment for ecosystem services programs, and changes of agricultural activity. Communities in the Sierra of Oaxaca have reforested degraded lands, created community forest enterprises, and preserved the forest under conservation schemes like those proposed by the Mexican payment for ecosystem services programs. However, their sustainable management faces internal challenges and has become highly dependent on political and institutional decisions beyond their control.

关键词： Tropical and temperate forests Social forestry Land uses segmentation OBIA FRAGSTAT

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Potential of Bamboo Species Guadua trinii and Guadua angustifolia for Nanocellulose Production

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Journal of Renewable Materials 2024年第9期12卷 1541-1555页

作者： Agatha E.R.Prado Gárate Fernando E.Felissia mar.a Cristina Area Teresa Suirezs mar.a Evangelina Vallejos Círculo de Investigación del Bambú Universidad Nacional Agraria La Molina(UNALM)Lima15026Perú Instituto de Materiales de Misiones(IMAM) Universidad Nacional de Misiones(UNaM)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Facultad de Ciencias ExactasQuímicas y Naturales(FCEQYN)Programa de Celulosa y Papel(PROCyP)Posadas3300Argentina Instituto de Materiales de Misiones(IMAM) Universidad Nacional de Misiones(UNaM)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Facultad de Ciencias Forestales(FCF)Eldorado3380Argentina

Non-traditional lignocellulosic materials are a significant resource for producing high-value products,including *** work studied the nanocellulose obtention from chemical pulps of the two fast-growing bamboo species,Guadua trinii,and Guadua *** pulps were produced by soda-anthraquinone(S)pulping from both autohydrolysis-pretreated(H)and unpretreated bamboo ***-pretreated(SHP)and unpretreated soda-anthraquinone(AQ)(SP)pulps were characterized by yield,Kappa number,alpha,beta,and gamma cellulose,degree of polymerization,water retention value,and *** nanocellulose was produced by a sequential chemical oxidation treatment(2,2,6,6-tetramethylpiperidine-1-oxyl,TEMPO reagent)and mechanical *** was characterized by carboxylic group content and *** results revealed that autohydrolysis pretreatment resulted in a higher hemicelluloses extraction in *** contrast,the pulping yield of unpretreated samples was higher for ***,and the soda-AQ pulps from this species exhibited better delignification than the autohydrolysis-pretreated *** crystallinity index values of the obtained pulps were high(>80%),and the alpha-cellulose contents were *** viscosities of the aqueous nanocellulose suspensions were higher for the nanocellulose solutions obtained from the unpretreated soda-AQ ***,nanocellulose from *** unpretreated soda-AQ pulps had a higher rate of carboxylic *** results of this work are significant in assessing the potential of both bamboo species as a source of high-value products within the biorefinery framework because the viscosities of the aqueous nanocellulose suspensions depend on the size and shape of *** has significant importance for industrial unit operations and potential applications.

关键词： Natural fiber Guadua angustifolia Guadua trinii soda-anthraquinone pulp nanocellulose

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From liver to hormones:The endocrine consequences of cirrhosis

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World Journal of Gastroenterology 2024年第9期30卷 1073-1095页

作者： Juan Eduardo Quiroz-Aldave Elman Rolando Gamar.a-Osorio mar.a del Carmen Durand-Vásquez Luciana del Pilar Rafael-Robles Jhean Gabriel Gonzáles-Yovera mar.a Alejandra Quispe-Flores Luis Alberto Concepción-Urteaga Alejandro Román-González José Paz-Ibarra marcio José Concepción-Zavaleta Non-communicable Diseases Endocrinology Research LineHospital de Apoyo ChepénChepén 13871Peru Department of Endocrinology Hospital Víctor Lazarte EchegarayTrujillo 13006Peru Department of Endocrinology Clínica Javier PradoLima 15046Peru Department of Gastroenterology Hospital Nacional Guillermo Almenara IrigoyénLima 15018Peru Department of Endocrinology Hospital de Emergencias GrauLima 15001Peru Internal Medicine Hospital Regional Docente de TrujilloTrujillo 13011Peru Department of Endocrinology Hospital Universitario de San Vicente FundaciónMedellin 050010Colombia Internal Medicine Universidad de AntioquiaMedellín 050010Colombia School of Medicine Universidad Nacional Mayor de San MarcosLima 15081Peru Department of Endocrinology Hospital Nacional Edgardo Rebagliati MartinsLima 15072Peru Carrera de Medicina Humana Universidad Científica del SurLima 15067Peru

Hepatocrinology explores the intricate relationship between liver function and the endocrine *** liver diseases such as liver cirrhosis can cause endocrine disorders due to toxin accumulation and protein synthesis *** its importance,assessing endocrine issues in cirrhotic patients is frequently *** article provides a comprehensive review of the epidemiology,pathophysiology,diagnosis,and treatment of endocrine disturbances in liver *** review was conducted using the PubMed/Medline,EMBASE,and Scielo databases,encompassing 172 *** cirrhosis is associated with endocrine disturbances,including diabetes,hypoglycemia,sarcopenia,thyroid dysfunction,hypogonadotropic hypogonadism,bone disease,adrenal insufficiency,growth hormone dysfunction,and secondary *** optimal tools for diagnosing diabetes and detecting hypoglycemia are the oral glucose tolerance test and continuous glucose monitoring system,*** can be assessed through imaging and functional tests,while other endocrine disorders are evaluated using hormonal assays and imaging *** options include metformin,glucagon-like peptide-1 analogs,sodium-glucose co-transporter-2 inhibitors,and insulin,which are effective and safe for diabetes *** standards are followed for managing hypoglycemia,and hormone replacement therapy is often necessary for other endocrine *** transplantation can address some of these problems.

关键词： Diabetes mellitus Hypoglycemia Thyroid diseases Hypogonadism Metabolic bone diseases Liver cirrhosis

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Efficient up-conversion photoluminescence in all-inorganic lead halide perovskite nanocrystals

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Nano Research 2020年第7期13卷 1962-1969页

作者： Andres granados del Aguila TThu Ha Do Jun Xing Wen Jie Jee Jacob B.Khurgin Qihua Xiong Division of Physics and Applied Physics School of Physical and Mathematical SciencesNanyang Technological UniversitySingapore 637371Singapore Key Laboratory of Eco-Chemical Engineering Ministry of EducationCollege of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdao 266042China Department of Electrical and Computer Engineering Johns Hopkins UniversityBaltimoreMaryland 21218USA State Key Laboratory of Low-Dimensional Quantum Physics Department of PhysicsTsinghua UniversityBeijing 100084China

Up-conversion photoluminescence(UCPL)refers to the elementary process where low-energy photons are converted into high-energy ones via consecutive interactions inside a *** additional energy is provided by intermnal thermal energy in the form of lttice vibrations(phonons),the process is called phonon-assisted ***,we report the exceptionally large phonon-assisted energy gain of up to^8kgT(kg is Boltzmann constant,T is temperature)on all-inorganic lead halide perovskite semiconductor colloidal nanocrystals that goes beyond the maximum capabilty of only harvesting optical phonon *** systematic optical study in combination with a statistical probability model,we explained the nontrivial phonon-assisted UCPL process in perovskites nanocrystals,where in addition to the strong electron-phonon(light-matter)coupling,other nonlinear processes such as phonon-phonon(matter-matter)interaction also effectively boost the up-conversion efficiency.

关键词： up-conversion photoluminescence phonons lead halide perovskite colloidal nanocrystals

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