检索结果-南通市图书馆

Effect of Red <i>Panax ginseng</i>on Mitochondrial Dynamics and Bioenergetics in HaCaT Cells Exposed to Urban Pollutants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Cosmetics, Dermatological Sciences and Applications 2021年第2期11卷 84-95页

作者： Gallic Beauchef magali Favre-Mercuret Beatrice Blanc Richard Fitoussi Katell Vié Nathalie Compagnone Clarins Research laboratory Pontoise France ICDD-sas Gemenos France Bliive Consulting Auriol France

Background: Urban air pollution contributes to lung and cardiovascular system dysfunction, making it a major concern for human health. Its impact on skin integrity, associated with increased occurrence of atopic dermatitis, is now recognized, but its cellular mechanisms remain poorly understood. Objective: In the present study we aimed at establishing the impact of urban pollutant on mitochondrial dynamics and bioenergetics using the HaCaT cell model. We also sought to establish the protective effect of ECH-5195 (red Panax ginseng extract), standardized in ginsenosides, in reversing pollution-induced mitochondrial defects. Methods: Urban pollution exposure was mimicked by 1 h exposure of HaCaT cells with standardized atmospheric particulate matter containing PAHs, nitro-PAHs, PCB congeners, and chlorinated pesticides with a mean particulate diameter of 5.85 μm (SRM1648). Results: The presence of urban pollutant in the cultures increased the prevalence of hyperfission by 1.41-fold (p = 0.023) and fission by 1.35 fold (p = 0.006) in the reticular mitochondrial network. ECH-5195 reduced both pollution-induced hyperfission by 0.54-fold (p = 0.004) and fission by 0.68-fold (p = 0.0006) normalizing the mitochondrial reticular network. Pollution exposure was associated with a significant reduction of basal OCR and increased lactate production, pushing the cell to rely on glycolysis for ATP production. When ECH-5195 was used, OCR was significantly increased, and the glycolytic contribution to ATP production was reduced while both oxidative phosphorylation and mitochondrial respiration were increased demonstrating mitochondrial re-engagement in ATP production. Conclusions: Pollution exposure was disruptive for both the mitochondrial network dynamics and mitochondrial respiration. Ginsenosides in ECH-5195 efficiently protected both from pollution-induced defects.

关键词： Ginsenosides Arylhydrocarbon Receptor Particulate Matter HaCaT Cells Mitochondria Dynamics Cell Bioenergetics

Fanconis贫血病人造血干细胞DNA修复基因hHR21^(sp)表达研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国病理生理杂志 2001年第5期17卷 388-391页

作者：邓宇斌李树浓周平坤 magali T 中山医科大学病理生理学教研室广东广州510080 军事医学科学院院放射医学研究所北京100850 法国巴黎圣露易医学院法国巴黎75010

目的 :研究正常人外周血单核细胞在UV或γ辐射后hHR2 1sp基因的转录表达 ,进一步分析hHR2 1sp基因在fanconisanemia(FA)单核细胞和造血干细胞的转录表达水平及发病机制中的作用。方法 :对正常人外周血单核细胞在UV或γ辐射后不同时间提... 详细信息

目的 :研究正常人外周血单核细胞在UV或γ辐射后hHR2 1sp基因的转录表达 ,进一步分析hHR2 1sp基因在fanconisanemia(FA)单核细胞和造血干细胞的转录表达水平及发病机制中的作用。方法 :对正常人外周血单核细胞在UV或γ辐射后不同时间提取细胞总RNA ,通过RT -PCR、southern杂交、以 β -actin为内参照放射影像对hHR2 1sp基因的转录表达水平进行检测 ,进一步检测FA病人外周血单核细胞和骨髓造血细胞hHR2 1sp基因的表达水平。结果 :正常外周血单核细胞在UV或γ -辐射后不同时间hHR2 1sp基因转录表达都有所变化。正常人外周血单核细胞与对照组比较在 80J/m2 UV辐射hHR2 1sp表达于 3h开始增加 ;在 6h表达水平最高 ,与正常对照有 2倍多差异 ,而在 9h表达有所降低 ;而γ -辐射 5Gy辐射 6h增加明显 ,在 9h后有所降低与正常对照有 2倍差异。检测发现FA病人单核细胞与正常人外周血单核细胞hHR2 1sp基因表达无明显差异 ;但FA病人造血干细胞与正常组骨髓细胞相比hHR2 1sp基因表达降低显著 ,有 1倍多差异。结论 :实验结果表明hHR2 1sp在FA病人造血干细胞表达缺陷 ,提示hHR2 1sp基因表达降低可影响FA病人造血干细胞和DNA双链断裂修复功能是FA发病机制之一。

关键词：基因表达电离辐射 hHR21^sp Fanconis贫血造血干细胞 DNA

Levels and trends of current-use pesticides(CUPs)in the arctic:An updated review,2010-2018

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Emerging Contaminants 2019年第1期5卷 70-88页

作者： Jennifer E.Balmer Adam D.Morris Hayley Hung Liisa Jantunen Katrin Vorkamp Frank Riget Marlene Evans magali Houde Derek C.G.Muir Arctic Monitoring and Assessment Programme Fram CentrePO Box 6606Langnes9296TromsøNorway Department of Biology The Citadel171 Moultrie StreetCharlestonSCUSA Environment and Climate Change Canada National Wildlife Research CenterOttawaCanada Air Quality Processes Research Section Environment and Climate Change CanadaTorontoOntarioCanada Air Quality Processes Research Section Environment and Climate Change CanadaEgbertOntarioCanada Aarhus University Department of Environmental ScienceArctic Research CentreRoskildeDenmark Aarhus University Department of BioscienceRoskildeDenmark Aquatic Ecosystem Protection Research Division Environment Canada11 Innovation BlvdSaskatoonSKS7N 3H5Canada Environment and Climate Change Canada St.Lawrence CenterMontrealQuebecCanada Environment and Climate Change Canada Canada Centre for Inland WatersBurlingtonOntarioCanada

Global regulations and many regional and national controls restrict the use of substances that exhibit the potential for environmental persistence and long-range ***,many current-use pesticides(CUPs)continue to be newly discovered in remote regions,including the *** present review serves as an update,summarizing newly available information for CUPs in the Arctic environment and biota published from 2010 to *** 2010,at least seven new CUPs have been measured in Arctic media:2-methyl-4-chlorophenoxyacetic acid(MCPA),metribuzin,pendimethalin,phosalone,quizalofop-ethyl,tefluthrin and *** the large number of pesticides in current use,the number measured in the Arctic is very limited,however,modelling studies have identified additional CUPs as potential Arctic contaminants that have yet to be investigated in the *** to their recent detection,reports of CUPs in the Arctic are limited,but *** have been reported in a wide range of abiotic Arctic matrices,including air,snow,ice,freshwater and seawater,indicating their capacity for long-range atmospheric transport,however,concentrations are generally low in comparison to legacy pesticides and other persistent organic pollutants(POPs).Recent food-web studies indicate CUPs can enter Arctic terrestrial and marine food chains,however,in contrast to POPs,the highest concentrations of many CUPs were found in lower trophic-level organisms,and the lowest concentrations detected in animals at the highest trophic levels(i.e,ringed seals,polar bear,caribou,and wolves)indicating significant trophic *** detection of CUPs in the remote Arctic ecosystem reinforces the need for continued monitoring of both known and potential Arctic pollutants to prevent impacts on human and environmental health as the global arsenal of pesticides used in agriculture continuously changes.

关键词： POPs Contaminants Air Biota Long-range transport Persistence

Evolutionary Genetics of Invasive Mammal Species Introduced in Argentina

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Natural Science 2018年第7期10卷 221-231页

作者： Marta S. Lizarralde Mariana Fasanella Sebastián Poljak magali Gabrielli Julieta Sanchez Lab de Ecologia Molecular Centro Austral de Investigaciones Científicas CADIC-CONICET. Av. Houssay Ushuaia Argentina Lab. Ecologia Molecular Centro Regional de Estudios Genómicos-FCE-UNLP. La Plata Argentina Lab. Ecotono Instituto de Investigaciones en Biodiversidad y Medio Ambiente INIBIOMA-CONICET. Bariloche Argentina ICPA Universidad Nacional de Tierra del Fuego Ushuaia. Tierra del Fuego Argentina

Evolutionary genetics of invasive species has been unexplored in Argentina. Invasive alien species (IAS) have a wide geographical distribution, characteristic life cycles and great ability to adapt, establish and spread in a new environment. Recent advances in novel molecular technologies, the use of higher resolution genetic markers, and the research development on genetic variation of invasive species consolidated the importance of genetic aspects in the invasion process. Undoubtedly, the growing concern for the disturbances generated by invasive species on biodiversity and functioning of ecosystems was also determinant for the inclusion of the Invasion Biology within the broad field of Evolutionary Biology including relevant examples that address the evolutionary genetic aspects of biological invasions. Recent studies suggest that the invasion success of many species depends on their ability to respond to natural selection. Although the number of invasive species registered in Argentina far exceeds 600, little research has been done on invasive mammal species and only five of them were hitherto genetically analyzed. Presuming that invasion genetics is incorporated into the agenda of control and management organizations, it would allow integrating the ecological, genetic, and evolutionary biology aspects for knowledge of invasive species widely distributed in Argentina. The objective of this article is to highlight the importance of evaluating the genetic structure of invasive species for their management and to inform about the invasive species of mammals that were introduced in Argentina and have been or are being analyzed genetically.

关键词： Evolutionary Genetics Invasive Mammals Argentina

辐射诱导人细胞系CEM、外周血单个核细胞DNA修复基因hHR21^(sp)的表达研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中华放射医学与防护杂志 2001年第6期21卷 413-416页

作者：邓宇斌李树浓周平坤 Teyssier magali 中山医科大学病生教研室广州510089 北京放射医学研究所巴黎圣露易医学院

目的　研究辐射对人T淋巴细胞白血病细胞系 (CEM)、外周血单个核细胞的hHR2 1sp基因转录表达水平的影响及意义。方法　分别对人T淋巴细胞白血病细胞系CEM和正常人外周血单核细胞在UV或γ辐射后不同时间提取细胞总RNA ,通过RT PCR与hHR2 ... 详细信息

目的　研究辐射对人T淋巴细胞白血病细胞系 (CEM)、外周血单个核细胞的hHR2 1sp基因转录表达水平的影响及意义。方法　分别对人T淋巴细胞白血病细胞系CEM和正常人外周血单核细胞在UV或γ辐射后不同时间提取细胞总RNA ,通过RT PCR与hHR2 1sp 基因特异引物杂交 ,以 β actin为内参照放射影像密度扫描检测人T淋巴细胞白血病细胞系CEM、单核细胞DNA修复基因表达。结果　在UV 辐射、γ 辐射后早期 (3～ 6h) ,人T淋巴细胞白血病细胞系CEM和淋巴细胞hHR2 1sp基因的表达水平明显增加 ,照射后 6hhHR2 1sp基因的表达水平增加最多且UV辐射更明显 ,在晚期 (9h)降低。比较人细胞系CEM和淋巴细胞两者hHR2 1sp基因的表达水平 ,γ辐射 (3Gy)后淋巴细胞对hHR2 1sp基因表达高于人细胞系CEM ,且表达增加时间较长 ,达 9h ;而人细胞系CEM受到γ辐射后早期表达增加 ,在 6～ 9h后表达降低。结论　人T淋巴细胞白血病细胞系 (CEM)和人淋巴细胞的DNA修复基因hHR2 1sp基因在一定剂量辐射 (UV、γ辐射 )范围内其表达水平随辐射剂量增加而诱导表达水平增高 ,且对UV辐射更敏感 ;提示hHR2 1sp基因在人细胞系CEM细胞和人单核细胞在照射损伤后表达增加 ,可能是促进单核细胞损伤修复的原因之一。

关键词：电离辐射基因表达 hHR21^sp基因

Ionic Liquids Mediated Ionothermal Process for the One-Step Synthesis of High Surface Area Alumina Supported Noble Metals

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Modern Research in Catalysis 2013年第2期2卷 28-35页

作者： magali Bonne Pierrick Gaudin Yanlong Gu Francois Jerome Yannick Pouilloux Daniel Duprez Sebastien Royer Universite de Haute Alsace(UHA) CNRSEquipe Materiaux a Porosite Controlee(MPC)Institut de Science des Materiaux de Mulhouse(IS2M)MulhouseFrance Universite de Poitiers Institut de Chimie des Milieux et Materiaux de Poitiers(IC2MP)PoitiersFrance Institute of Physical Chemistry and Industrial Catalysis Department of Chemistry and Chemical EngineeringUniversity of Science and TechnologyWuhanChina

Aluminas supported noble metal nanoparticles have been synthesized by a new ionothermal process. The method used is easy to implement and allows obtaining catalysts in one step with high specific surface area. In addi... 详细信息

关键词： Mesostructuration Alumina Noble Metals Ionothermal Process EISA

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

全民太空游近在眼前?

新发现 2021年第9期 14-22页

作者： magali Rebeaud 杨冉(编译) 不详

太空探索一直让人为之着迷。而2021年则是太空旅游划时代的一年。全球两大富豪贝索斯和马斯克都宣称已做好将第一批“游客”送往太空的准备。人人游太空的时代真的来了?

关键词：太空旅游太空探索大富豪贝索斯划时代

SARS-CoV-2 spike RBD-specific IgA and IgG antibodies in breast milk after vaccination with the protein subunit vaccine Abdala

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Infectious Medicine 2022年第4期1卷 253-261页

作者： Maylin Pérez-Bernal Carlos Hernández Rafael Ibargollín Midalis Martínez Migdiala Soria magali Delgado Onel Valdivia DayamíDorta Andy Domínguez Enrique Pérez Yeosvany Cabreraa Research&Development Department Center for Genetic Engineering and BiotechnologyCircunvalante NorteOlivos IIISancti SpiritusCuba Neonatology Service General Hospital“Camilo Cienfuegos”128 Bartolome MasoSancti SpiritusCuba Production Department Center for Genetic Engineering and BiotechnologyCircunvalante NorteOlivos IIISancti SpiritusCuba

Background:COVID-19 vaccines that trigger a strong secretory antibody response in breast milk may achieve effective passive protection of vulnerable newborns and breastfed infants of immunized *** aim of this work was to investigate the presence of SARS-CoV-2 spike RBD-specific IgA and IgG antibodies in breast milk,5 and 9 weeks after vaccination with 3 doses of the protein subunit vaccine Abdala,compared to those found in breast milk from COVID-19-recovered women,collected at least 40 days after the ***:SARS-CoV-2 spike RBD-specific IgA and IgG antibodies were semi-quantified by indirect ELISA,using a homemade standard generated by pooling twenty breast milk samples with high absorbance values according to preliminary *** validity of the standard curves was proved following the European Medicines Agency *** breast milk samples from 2 unvaccinated women who had not been infected with COVID-19 were included as negative *** neutralizing antibodies was assessed by a SARS-CoV-2 surrogate virus neutralization ***:High levels of anti-RBD IgA antibodies were detected in breast milk samples 9 weeks after vaccination and anti-RBD IgG antibodies rise from the fifth to the ninth *** the post-COVID-19 time that was evaluated,the IgG-type response was notably higher compared to both post-vaccination *** antibody titers were similar in breast milk from vaccinated and COVID-19 recovered ***:This is the first report about the immune response in breast milk after the administration of a COVID-19 protein subunit vaccine,which could provide analogous protection to that conferred by SARS-CoV-2 *** implies a potential passive immunity that breastfed infants receive from their mothers vaccinated with Abdala.

关键词： Abdala vaccine IgA IgG Neutralizing antibody titers SARS-CoV-2

Clustering of prostate cancer healthcare pathways in the French National Healthcare database

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cancer Innovation 2023年第1期2卷 52-64页

作者： Roméo Baulain Jérémy Jové Dunia Sakr Marine Gross‐Goupil magali Rouyer Marius Puel Patrick Blin Cécile Droz‐Perroteau Régis Lassalle Nicolas H.Thurin École nationale de la statistique et de l'administrationéconomique Paris(ENSAE) Institut Polytechnique ParisPalaiseauFrance Univ.Bordeaux INSERM CIC‐P 1401Bordeaux PharmacoEpiBordeauxFrance Medical Oncology Hôpital Saint AndréCHU de BordeauxBordeauxFrance

Background:Healthcare pathways of patients with prostate cancer are heterogeneous and complex to apprehend using traditional descriptive *** and visualization methods can enhance their ***:Patients with prostate cancer in 2014 were identified in the French National Healthcare database(Système National des Données de Santé—SNDS)and their data were extracted with up to 5 years of history and 4 years of follow‐***‐one‐specific encounters constitutive of prostate cancer management were synthesized into four macro‐variables using a clustering *** values over patient follow‐ups constituted healthcare *** matching was applied to calculate distances between *** around medoids was then used to define consistent groups across four exclusive cohorts of incident prostate cancer patients:Hormone‐sensitive(HSPC),metastatic hormone‐sensitive(mHSPC),castration‐resistant(CRPC),and metastatic castration‐resistant(mCRPC).Index plots were used to represent pathways ***:The repartition of macro‐variables values—surveillance,local treatment,androgenic deprivation,and advanced treatment—appeared to be consistent with prostate cancer *** to five clusters of healthcare pathways were observed in each of the different cohorts,corresponding for most of them to relevant clinical patterns,although some heterogeneity *** instance,clustering allowed to distinguish patients undergoing active surveillance,or treated according to cancer progression risk in HSPC,and patients receiving treatment for potentially curative or palliative purposes in mHSPC and ***:Visualization methods combined with a clustering approach enabled the identification of clinically relevant patterns of prostate cancer *** of these care pathways is an essential element for the comprehension and the robust assessment of healthcare technology effectiveness.

关键词： prostate cancer healthcare pathway clustering machine learning SNDS