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cell Research 1997年第2期7卷 207-215页

作者： WU YA lan guo HUANG FAN JIAN ZHAO YIZHANG TIAN HUA ZHOU lan ma GANG PEI(shanghai institute of cell biology. Chinese Academy ofSciences, shanghai 200031, Chinashanghai Medical University, shanghai 200032, China) shanghai Medical University Shanghai 200032 China

Expression of opioid receptor-like receptor (ORL1)and its endogenous peptide agonist nociceptin/orphaninFo (N/OFQ) during mouse embryogenesis have been investigated. Transcripts of ORL1 and N/OFQ were detected by RT-PCR in mouse brain of day 8 embryo (E8)and the expression continued afterwards. Northern blotanalysis revealed abundant expression of ORL1 at postnatal day 1 (P1) and N/OFQ at E17 and P1 in the brain butnone was detected in other embryonic tissues. The presence of functional ORL1 in mouse embryonic brain wasalso confirmed by specific binding of [3H] N/OFQ (kd=1.3±0.5 nM and Bmax = 72±9 fmol/mg protein) as wellas by N/OFQ-stimulated G protein activation.

关键词： Opioid receptor-like receptor (ORL1) nociceptin/orphanin FQ (N/OFQ) mouse embryogenesis functional expression

Wuzi Yanzong Pill Plays A Neuroprotective Role in Parkinson’s Disease Mice via Regulating Unfolded Protein Response Mediated by Endoplasmic Reticulum Stress

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Chinese Journal of Integrative Medicine 2023年第1期29卷 19-27页

作者： LI Yan-rong FAN Hui-jie SUN Rui-rui JIA Lu YANG Li-yang ZHANG Hai-fei JIN Xiao-ming XIAO bao-guo ma Cun-gen CHAI Zhi The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine Shanxi University of Chinese MedicineJinzhongShanxi Province030619China institute of Brain Science Department Neurology of First Affiliated HospitalShanxi Datong UniversityDatongShanxi Province037009China Department of Anatomy and cell biology Department of Neurological SurgerySpinal Cord and Brain Injury Research GroupStark Neurosciences Research InstituteIndiana University School of MedicineIndianapolisIN46202USA institute of Neurology Huashan HospitalInstitutes of Brain Science and State Key Laboratory of Medical NeurobiologyFudan UniversityShanghai200025China

Objective:To investigate the protective effects and its possible mechanism of Wuzi Yanzong Pill(WYP) on Parkinson’s disease(PD) model ***:Thirty-six C57BL/6 male mice were randomly assigned to 3 groups including normal,PD,and PD+WYP groups,12 mice in each *** week of intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) was used to establish the classical PD model in ***,mice in the PD+WYP group were administrated with 16 g/kg WYP,twice daily by *** 14 days of administration,gait test,open field test and pole test were measured to evaluate the movement *** hydroxylase(TH) neurons in substantia nigra of midbrain and binding immunoglobulin heavy chain protein(GRP78) in striatum and cortex were observed by *** levels of TH,GRP78,p-PERK,p-elF2α,ATF4,p-IRE1α,XBP1,ATF6,CHOP,ASK1,p-JNK,Caspase-12,-9 and-3 in brain were detected by Western ***:Compared with the PD group,WYP treatment ameliorated gait balance ability in PD mice(Pcells(Pmay be related to the regulation of unfolded protein response-mediated signaling pathway and inhibiting the endoplasmic reticulum stress-mediated neuronal apoptosis pathway.

关键词： Wuzi Yanzong Pill Parkinson’s disease dopaminergic neurons endoplasmic reticulum stress apoptosis

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IPP5 inhibits neurite growth in primary sensory neurons by main-taining TGF-β/Smad signaling

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IPP5 inhibits neurite growth in primary sensory neurons by m...

Effect of FasL High Expression on Immune Regulative Function of Sertoli cell in Transgenic Mouse

中国神经科学学会第十届全国学术会议

作者： Qing-Jian Han Nan-Nan Gao guo-qiang ma Zhen-Ning Zhang Wen-Hui Yu Jing Pan Qiong Wang Xu Zhang lan bao State Key Laboratory of cell biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of Sciences institute of Neuroscience and State Key Laboratory of Neuroscience Shanghai Institutes for Biological SciencesChinese Academy of Sciences

Objective Neurite growth is a critical step for successful nerve regeneration after injury and it is regulated by both intrinsic molecules and extracellular *** found that inhibitor 5 of protein phosphatase 1(IPP5),a newly identified inhibitory subunit of protein phosphatase 1(PP1),was highly expressed in rat primary sensory neurons localized in the dorsal root ganglion(DRG),specifically in small primary sensory neurons,and was downregulated after sciatic nerve axotomy,and may serve as a neurite growth inhibitory molecule in primary sensory *** we explore the regulatory functions of IPP5 in DRG neurite ***(1) Knocking down IPP5 with specific shRNA and examining the neurite growth of cultured DRG neurons;(2) Mutating the PP1 binding motif or the PP1inhibiting motif at Thr to determine whether the inhibition of PP1 is responsible for the effect of IPP5 on neurite growth;(3) Blocking TGF-p signaling with TβRI inhibitor SB431542 or Smad2 shRNA to clarify the involvement of TGF-p signaling ***(1) Knocking down IPP5 promote neurite growth in cultured primary sensory neurons.(2) The interaction with and inhibition of PP1 is responsible for the effect of IPP5 on neurite growth.(3) IPP5inhibits neurite growth by regulating the TGF-p signaling *** IPP5 inhibits neurite growth in primary sensory neurons in a PP1-dependent manner and functions by maintaining TGF-p/Smad signaling pathway.

关键词： IPP5 neurite growth PP1 TGF-β/Smad signaling DRG neuron

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Journal of Reproduction and Contraception 2004年第4期15卷 209-218页

作者： Wei-yi LI Zhao-ping LIU Ye-bin XI Bo LIN Ying-hua ma Jia-hua HU Guang-jie CHEN bao-guo WANG Shi-san bao Li-he guo shanghai institute of Immunology Shanghai Second Medical University Shanghai 200025 China institute of biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Department of Veterinary Anatomy ＆ Pathology B14 University of Sydney NSW 2006 Australia

Objective To study the immune regulative function of Sertoli cell on testis local infection Methods Ureaplasma urealyticum （UU） was directly injected into bladders of FasL transgenic mice and wild-type mice, which mimicked an ascending infectious way. At week 1, 2 and 3 after injection respectively, the mice were killed to observe the pathological alterations in testis section. And at the same time cytokines was tested by immunohistochemistry. Comparison of levels of FasL, TGF-β, IL-1α and IL-6 between UU-infected and control groups of wild mice and FasL transgenic mice was made respectively. Then the capability of Sertoli cell （FasL^＋） to mediate apoptosis of Fas^＋ cells between wild control and wild UU-infected groups was analyzed. Results The pathological changes of testis in FasL transgenic mice were more seriously compared with wild counterpart and the changing mode of cytokines secreted by Sertoli cells were different between the two kinds of mice. The UU-infected Sertoli cells increased Fas^＋ Jurkat cell apoptosis. Conclusions High expression of FasL in FasL transgenic mice can influence the cytokines secretion during anti-infection, thus affecting the testis immune response to infection and immune balance. The high expression of FasL is not beneficial for body＇s anti-inflection immune response.

关键词： Sertoli cell immune regulative function FasL TGF-β IL-1α IL-6

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Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice

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Asian Journal of Andrology 2020年第6期22卷 590-601页

作者： Chen Chu Lu Yu Joelle Henry-Berger Yan-Fei Ru Ayhan Kocer Alexandre Champroux Zhi-Tong Li Miao He Sheng-Song Xie Wu-Bin ma Min-Jie Ni Zi-Mei Ni Yun-Li guo Zhao-Liang Fei lan-Tao Gou qiang Liu Samanta Sharma Yu Zhou Mo-Fang Liu Charlie Degui Chen Andrew L Eamens Brett Nixon Yu-Chuan Zhou Joel R Drevet Yong-Lian Zhang State Key Laboratory of Molecular biology Shanghai Key Laboratory of Molecular AndrologyCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China Genetics Reproduction and Development Laboratory CNRS UMR 6293-INSERM U1103-Universite Clermont AuvergneClermont-Ferrand 63001France institute of Neuroscience State Key Laboratory of NeuroscieneeShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China Key Laboratory of Agricultural Animal Genetics Breedingand Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of AgricultureHuazhong Agricultural UniversityWuhan 430070China Department of cellular and Molecular Medicine University of CaliforniaSan DiegoLa JollaCA 92093-0651USA Department of Anatomy Histology and EmbryologyShanghai Jiao long University School of MedicineShanghai Key Laboratory of Reproductive MedicineShanghai 200025China department of Cancer biology Dana-Farber Cancer InstituteBostonMA 02215USA Department of Genetics Harvard Medical SchoolBostonMA 02115USA Priority Research Centre for Reproductive Sciences School of Environmental and Life SciencesThe University of NewcastleCallaghanNSW 2308Australia.

The mammalian epididymis not only plays a fun dame ntal role in the maturati on of spermatozoa,but also provides protecti on agai nst various *** foremost among these is the threat posed by oxidative stress,which arises from an imbalance in reactive oxygen species and can elicit damage to cellular lipids,proteins,and nucleic *** mice,the risk of oxidative damage to spermatozoa is mitigated through the expression and secretion of glutathione peroxidase 5(GPX5)as a major luminal scavenger in the proximal caput epididymidal ***,the loss of GPX5^-/-mediated protection leads to impaired DNA integrity in the spermatozoa of aged Gpx57 *** explore the underlying mechanism,we have conducted transcriptomic analysis of caput epididymidal epithelial cells from aged(13 months old)Gpx5^-/-m *** analysis revealed the dysregulation of several thousand epididymal mRNA transcripts,in eluding the downregulation of a subgroup of piRNA pathway gen es,in aged Gpx5^-/-*** agreeme nt with these fin dings,we also observed the loss of piRNAs,which potentially bind to the P-element-induced wimpy testis(PlWI)-like proteins PIWIL1 and *** absence of these piRNAs was correlated with the elevated mRNA levels of their putative gene targets in the caput epididymidis of Gpx5^-/-***,the oxidative stress response genes tend to have more targeting piRNAs,and many of them were among the top increased genes upon the loss of GPX5^-/-.Taken together,our findings suggest the existence of a previously uncharacterized somatic piRNA pathway in the mammalian epididymis and its possible invoIvement in the aging and oxidative stress-mediated responses.

关键词： epididymis GPX5 oxidative stress piRNA PIWI-interacting RNA small noncoding RNA

Toripalimab plus chemotherapy as second-line treatment in previously EGFR-TKI treated patients with EGFR-mutant-advanced NSCLC:a multicenter phase-II trial

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Signal Transduction and Targeted Therapy 2021年第11期6卷 3295-3303页

作者： Tao Jiang Pingyang Wang Jie Zhang Yanqiu Zhao Jianying Zhou Yun Fan Yongqian Shu Xiaoqing Liu Helong Zhang Jianxing He Guanghui Gao Xiaoqian Mu Zhang bao Yanjun Xu Renhua guo Hong Wang Lin Deng Ningqiang ma Yalei Zhang Hui Feng Sheng Yao Jiarui Wu Luonan Chen Caicun Zhou Shengxiang Ren shanghai Pulmonary Hospital Tongji University School of MedicineShanghaiChina State Key Laboratory of cell biology Innovation Center for Cell Signaling NetworkCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghaiChina Henan Cancer Hospital ZhengzhouChina The First Affiliated Hospital of Zhejiang University HangzhouChina Zhejiang Cancer Hospital HangzhouChina Jiangsu Province Hospital NanjingChina The Fifth Medical Center of PLA General Hospital BeijingChina Tangdu Hospital Fourth Military Medical UniversityXi’anChina The First Affiliated Hospital of Guangzhou Medical University GuangzhouChina shanghai Junshi Biosciences Co.Ltd ShanghaiChina Key Laboratory of Systems Health Science of Zhejiang Province Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhouChina School of Life Science and Technology ShanghaiTech UniversityShanghaiChina

This multicenter phase-II trial aimed to investigate the efficacy,safety,and predictive biomarkers of toripalimab plus chemotherapy as second-line treatment in patients with EGFR-mutant-advanced *** who failed from first-line EGFR-TKIs and did not harbor T790M mutation were *** plus carboplatin and pemetrexed were administrated every three weeks for up to six cycles,followed by the maintenance of toripalimab and *** primary endpoint was objective-response rate(ORR).Integrated biomarker analysis of PD-L1 expression,tumor mutational burden(TMB),CD8+tumor-infiltrating lymphocyte(TIL)density,whole-exome,and transcriptome sequencing on tumor biopsies were also *** patients were enrolled with an overall ORR of 50.0%and disease-control rate(DCR)of 87.5%.The median progression free survival(PFS)and overall survival were 7.0 and 23.5 months,*** most common treatment-related adverse effects were leukopenia,neutropenia,anemia,ALT/AST elevation,and *** analysis showed that none of PD-L1 expression,TMB level,and CD8+TIL density could serve as a predictive *** analysis of whole-exome and transcriptome sequencing data revealed that patients with DSPP mutation had a decreased M2 macrophage infiltration and associated with longer PFS than those of wild *** plus chemotherapy showed a promising anti-tumor activity with acceptable safety profiles as the second-line setting in patients with EGFR-mutant *** mutation might serve as a potential biomarker for this combination.A phase-III trial to compare toripalimab versus placebo in combination with chemotherapy in this setting is ongoing(NCT03924050).

关键词： NSCLC chemotherapy treatment

Phenotypic and functional characteristic of a newly dentified CDS＋Foxp3-CDI03＋ regulatory T cells

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Journal of Molecular cell biology 2014年第1期8卷 81-92页

作者： Ya Liu Qin lan Ling Lu maogen Chen Zanxian Xia Jilin ma Julie Wang Huimin Fan Yi Shen Bernhard Ryffel David Brand Francisco Quismorio Zhongmin Liu David A. Horwitz Anping Xu Song guo Zheng Department of Nephrology Sun Yat-sen Memorial Hospital of Sun Yat-sen University Guangzhou 510120 China Division of Rheumatology and Immunology Department of Medicine University of Southern California Keck School of Medicine Los Angeles CA 90033 USA institute of Immunology Shanghai East Hospital at Tongji University Shanghai200120 China University of Orleans and CNRS UMR7355 3b rue de la Ferollerie Orleans 45071 France Veterans Affairs Medical Center Memphis TN 38104 USA

TGF-β and Foxp3 expressions are crucial for the induction and functional activity of CD4＋Foxp3＋ regulatory T （iTreg） cells. Here, we demonstrate that although TGF-β-primed CD8＋ cells display much lower Foxp3 expression, their suppressive capacity is equivalent to that of CD4＋ iTreg cells, and both Foxp3- and Foxp3＋ CD8＋ subsets have suppressive activities in vitro and in vivo. CD8＋Foxp3- iTreg cells produce little IFN-γ but almost no IL-2, and display a typical anergic phenotype. Among phenotypic markers expressed in CD8＋Foxp3- cells, we identify CD103 expression particularly crucial for the generation and function of this subset. Moreover, IL-IO and TGF-β signals rather than cytotoxicity mediate the suppressive effect of this novel Treg population. Therefore, TGF-β can induce both CD8＋Foxp3- and CD8＋Foxp3＋ iTreg subsets, which may represent the unique immunoregulatory means to treat autoimmune and inflammatory diseases.

关键词： CD8+ regulatory T cells TGF-β Foxp3 CD103

A digital microfluidic single-cell manipulation systemoptimized by extending-depth-of-field device

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Journal of Innovative Optical Health Sciences 2023年第3期16卷 43-55页

作者： Qiushu Chen Qi Meng Yuzhe Liu Xiangan Long Yawei Kong Longfang Yao Liwen Chen Chuanyong Wu Kaiqin Chu lan Mi Jiong ma Department of Optical Science and Engineering shanghai Engineering Research Center of Ultra-precision Optical manufacturing Key Laboratory of Micro and Nano Photonic Structures(Ministry of Education)School of Information Science and Technology Fudan University Shanghai 200433P.R.China Department of Precision machinery and Precision Instrumentation University of Science and Technology of China Hefei 230027 AnhuiP.R.China institute of Biomedical Engineering and Technology Academy for Engineering and Technology Fudan University Shanghai 200433P.R.China institute of Photonic Chips Centre for Articial-Intelligence Nanophotonics School of Optical-Electrical and Computer Engineering University of shanghai for Science and Technology shanghai 200093 P.R.China Ruidge Biotech Co.Ltd. No.888Huanhu West 2nd Road Lin-Gang Special AreaChina(Shanghai)Pilot Free Trade Zone Shanghai 200131P.R.China shanghai Hengxin BioTechnology Ltd.1688 North Guo Quan RdBldg A8Rm 801 Shanghai 200438P.R.China Centre for Articial-Intelligence Nanophotonics School of Optical-Electrical and Computer Engineering University of shanghai for Science and Technology shanghai 200093 P.R.China shanghai Engineering Research Center of Industrial Microorganisms The Multiscale Research Institute of Complex Systems(MRICS)School of Life SciencesFudan UniversityShanghai 200433P.R.China

Microfuidic systems have been widely utilized in high-throughput biology.analysis,but thedificulties in iquid manipulation and cell cultivation limit its *** work has developed a new digital microfluidic(DMF)system for on-demand droplet *** adopting anextending-depth-of-field(EDoF)phase modulator to the optical system,the entire depth of themicrofluidic channel can be covered in one image without any refocusing process,ensuring that 95%of the particles in the droplet are captured within three shots together with shaking *** this system,suspension droplets are generated and droplets containing only oneyeast cll can be recognized,then each single cell is cultured in the array of the *** growth in cell numbers and the green fluorescence protein(GFP)production via fluorescence imaging,the single cell with the highest production can be *** results haveproved the heterogeneity of yeast cells,and showed that the combined system can be applied forrapid single-cell sorting,cultivation,and analysis.

关键词： Single-cell analysis digital microfluidic(DMF) extending-depth-of-field system

eGPS 1.0: comprehensive software for multi-omic and evolutionary analyses

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National Science Review 2019年第5期6卷 867-869页

作者： Dalang Yu Lili Dong Fangqi Yan Hailong Mu Bixia Tang Xiao Yang Tao Zeng Qing Zhou Feng Gao Zhonghuang Wang Ziqian Hao Hongen Kang Yi Zheng Hongwei Huang Yuzhang Wei Wei Pan Yaochen Xu Junwei Zhu Shilei Zhao Ciran Wang Pengyu Wang Long Dai Mushan Li Li lan Yiwei Wang Hua Chen Yi-Xue Li Yun-Xin Fu Zhen Shao Yiming bao Fangqing Zhao Luo-Nan Chen guo-Qing Zhang Wenming Zhao Haipeng Li CAS Key Laboratory of Computational biology CAS-MPG Partner Institute for Computational Biology Shanghai Institute of Nutrition and HealthShanghai Institutes for Biological SciencesUniversity of Chinese Academy of Sciences Chinese Academy of Sciences University of Chinese Academy of Sciences BIG Data Center Beijing Institute of GenomicsChinese Academy of Sciences shanghai Center for Bioinformation Technology Key Laboratory of Systems biology Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences shanghai institute of biochemistry and cell biology Chinese Academy of Sciences CAS Laboratory of Genomic and Precision Medicine Beijing Institute of Genomics Chinese Academy of Sciences Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences Department of Biostatistics and Data Science School of Public Health University of Texas Health Science Center at Houston Computational Genomics Lab Beijing Institutes of Life Science Chinese Academy of Sciences shanghai Research Center for Brain Science and Brain-Inspired Intelligence

It has become increasingly challenging for researchers to analyze the exponentially expanding amount of multi-omic ***, we describe multi-functional software named evolutionary GenotypePhenotype Systems (eGPS) that en... 详细信息

关键词： GPS eGPS 1.0 comprehensive software for multi-omic and evolutionary analyses