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Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system(RAS)

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Journal of Oceanology and Limnology 2019年第1期37卷 350-360页

作者： WANG Yanfeng CHI Liang LIU Qinghua XIAO Yongshuang ma daoyuan XIAO Zhizhong XU Shihong LI Jun CAS Key Laboratory of Experimental marine Biology Institute of Oceanology Chinese Academy of Sciences Laboratory for marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Center for Ocean Mega-Science Chinese Academy of Sciences College of Veterinary Medicine Qingdao Agricultural University

Atlantic salmon reared in recirculating aquaculture system(RAS) may lead to inappropriately high stocking density, because ?sh live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the in?uence of stocking density on growth and some stress related physiological factors were investigated to evaluate the ef fects of stocking density. The ?sh were reared for 220 days at ?ve densities(A: 24 kg/m^3; B: 21 kg/m^3; C: 15 kg/m^3; D: 9 kg/m^3 and E: 6 kg/m^3). The results show that 30 kg/m^3 might be the maximum density which RAS can af ford in China. The stocking densities under 30 kg/m^3 have no ef fect on mortality of Atlantic salmon reared in RAS. However, the speci?c growth rate(SGR), ?nal weight and weight gain in the high density group were signi?cantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate(FCR) had a negative correlation with density. Plasma hormone T_3 and GH showed signi?cant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone(T 3), GH(growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT(glutamic oxalacetic transaminase) and GPT(glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no ef fects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise effcient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.

关键词： stocking density growth physiological parameters Atlantic salmon RAS

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A potential germ cell-specific marker in Japanese flounder,Paralichthys olivaceus：identification and characterization of lymphocyte antigen 75(Ly75/CD205)

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Journal of Oceanology and Limnology 2018年第4期36卷 1342-1348页

作者： YANG Yang LIU Qinghua ma daoyuan SONG Zongcheng LI Jun Key Laboratory of Experimental marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Laboratory for marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China University of Chinese Academy of Sciences Beijing 100049 China Weihai Shenghang Aquatic Product Science and Technology Co. Ltd. Weihai 264200 China

Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog( ly75) was cloned and its expression pattern in gonads was analyzed. The full-length c DNA of ly75 was 7 346 bp, with an open reading frame(ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 k Da. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.

关键词： Ly75/CD205 Japanese flounder Paralichthys olivaceus gonads germ cell-specific marker

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The ontogeny of acidic digestive tract in larval turbot Scophthalmus maximus based on H+/K+-ATPase and pepsinogen

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Journal of Oceanology and Limnology 2023年第3期41卷 1100-1108页

作者： Yanfeng WANG Shulan LIU Teng GUO Shihong XU Qinghua LIU Zhizhong XIAO daoyuan ma Jun LI Liang CHI Key Laboratory of Experimental marine Biology Centre for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Qingdao Agricultural University Qingdao 266109China Laboratory for marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Southern marine Science and Engineering Guangdong Laboratory Guangzhou 511458China Conson Oriental(Yantai)Recirculating Aquaculture Technology Co. Ltd.Yantai 264000China

Acidic digestion is an important digestive process of marine *** fish stomach,two enzymes are involved in the secretion of hydrochloric acid(HCl)and proteomic digestion:H^(+)/K^(+)-ATPase and ***,the starting of digestive function in fish is still *** reveal the details of acidic digestion of turbot Scophthalmus maximus in early development,a 40 day of turbot larvae culture was *** H^(+)/K^(+)-ATPase gene from the turbot ***(smH^(+)/K^(+)-ATPase)was identified and *** on our previous discription on pepsinogen of turbot ***,we combined pepsinogen and H^(+)/K^(+)-ATPase and analyzed the mechanism of acidic digestion in *** show that the spatial and temporal expression profiles of H^(+)/K^(+)-ATPase agreed with pepsinogen A and C in turbot,indicating a synergetic action between H^(+)/K^(+)-ATPase and pepsinogen during the acidic digestion *** addition,the turbot juveniles showed a faster growth after the expressions of H^(+)/K^(+)-ATPase gene and pepsinogen gene,demonstrating that pepsin had a higher digestive efficiency,for which a compound diet should be provided to the fish from Day 22 *** study provided a reference for biology research and aquaculture of turbot and other marine fishes.

关键词： turbot Scophthalmus maximus acidic digestion H+/K+-ATPase pepsinogen

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白光LED用Mn^(4+)激活氟氧化物红色荧光粉研究进展

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发光学报 2023年第11期44卷 1904-1922页

作者：马道远刘云正夏李斌江西理工大学能源与机械工程学院江西南昌330013

近年来,白光LED因其节能、环保、长寿命等优点已成为市场主流照明。高性能红色荧光粉是改善白光LED显色性的重要材料。Mn^(4+)激活氟氧化物红色荧光粉兼具了氟化物的良好发光性能和氧化物的高稳定性,当前已成为了一个研究热点。本文综... 详细信息

近年来,白光LED因其节能、环保、长寿命等优点已成为市场主流照明。高性能红色荧光粉是改善白光LED显色性的重要材料。Mn^(4+)激活氟氧化物红色荧光粉兼具了氟化物的良好发光性能和氧化物的高稳定性,当前已成为了一个研究热点。本文综述了多种以Mn^(4+)为激活剂的氟氧化物红色荧光粉,从晶体场理论以及热猝灭机理的角度出发,分类详述了各荧光粉晶体结构对发光性能的影响关系,以期为改善Mn^(4+)激活氟氧化物荧光粉发光性能提供理论指导。最后总结了Mn^(4+)激活氟氧化物红色荧光粉的优缺点和研究中存在的问题,并对未来的发展趋势进行了展望。

关键词：发光材料 Mn^(4+) 氟氧化物红色荧光粉白光LED

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基于Eu^(2+)和Mn^(2+)激活的背光源显示用窄带绿光发射荧光粉

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硅酸盐学报 2023年第12期51卷 3240-3250页

作者：刘云正马道远夏李斌江西理工大学能源与机械工程学院

在背光源液晶显示(LCD)领域,窄带绿光发射荧光粉对于扩宽显示色域、提高发光强度、优化显色性能等具有重要的作用。近年来,针对LCD用窄带绿光发射荧光粉有着广泛的研究,也取得良好的进展,然而对此类荧光粉的系统性总结却鲜有报道。本文... 详细信息

在背光源液晶显示(LCD)领域,窄带绿光发射荧光粉对于扩宽显示色域、提高发光强度、优化显色性能等具有重要的作用。近年来,针对LCD用窄带绿光发射荧光粉有着广泛的研究,也取得良好的进展,然而对此类荧光粉的系统性总结却鲜有报道。本文综述了近年来绿色窄带发射荧光粉的研究进展,通过对不同的激活离子进行了分类,重点介绍了以Eu^(2+)、Mn^(2+)做激活离子为代表的荧光粉,归纳分析了荧光粉结构和发光性能的关系,并阐述了各自的优缺点,同时对其未来发展前景进行了展望。

关键词：背光源显示液晶显示窄带发射绿色荧光粉

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Aging hallmarks of the primate ovary revealed by spatiotemporal transcriptomics

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Protein & Cell 2024年第5期15卷 364-384页

作者： Huifen Lu Ying Jing Chen Zhang Shuai ma Weiqi Zhang daoyuan Huang Bin Zhang Yuesheng Zuo Yingying Qin Guang-Hui Liu Yang Yu Jing Qu Si Wang Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China Institute for Stem cell and Regeneration CASBeijing 100101China China National Center for Bioinformation Beijing 100101China The Fifth People’s Hospital of Chongqing Chongqing 400062China Sino-Danish College University of Chinese Academy of SciencesBeijing 101408China Sino-Danish Center for Education and Research Beijing 101408China Department of Obstetrics and Gynecology Center for Reproductive MedicinePeking UniversityThird HospitalBeijing 100191China Clinical Stem Cell Research Center Peking UniversityThird HospitalBeijing 100191China Center for Reproductive Medicine Cheeloo College of MedicineShandong UniversityJinan 250012China Key Laboratory of Reproductive Endocrinology of Ministry of Education National Research Center for Assisted Reproductive Technology and Reproductive GeneticsShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthJinan 250012China Key Laboratory of Organ Regeneration and Reconstruction Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Biomarker Consortium Beijing 100101China

The ovary is indispensable for female reproduction,and its age-dependent functional decline is the primary cause of ***,the molecular basis of ovarian aging in higher vertebrates remains poorly ***,we apply spatiotemporal transcriptomics to benchmark architecture organization as well as cellular and molecular determinants in young primate ovaries and compare these to aged primate *** a global view,somatic cells within the non-follicle region undergo more pronounced transcriptional fluctuation relative to those in the follicle region,likely constituting a hostile microenvironment that facilitates ovarian ***,we uncovered that inflammation,the senescent-associated secretory phenotype,senescence,and fibrosis are the likely primary contributors to ovarian aging(PCOA).Of note,we identified spatial co-localization between a PCOA-featured spot and an unappreciated MT2(Metallothionein 2)highly expressing spot(MT2^(high))characterized by high levels of inflammation,potentially serving as an aging hotspot in the primate ***,with advanced age,a subpopulation of MT2^(high)accumulates,likely disseminating and amplifying the senescent signal *** study establishes the first primate spatiotemporal transcriptomic atlas,advancing our understanding of mechanistic determinants underpinning primate ovarian aging and unraveling potential biomarkers and therapeutic targets for aging and age-associated human ovarian disorders.

关键词： spatial transcriptome primate ovary aging senescence inflammation

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CRL2^(APPBP2)-mediated TSPYL2 degradation counteracts human mesenchymal stem cell senescence

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Science China(Life Sciences) 2024年第3期67卷 460-474页

作者： daoyuan Huang Qian Zhao Kuan Yang Jinghui Lei Ying Jing Hongyu Li Chen Zhang Shuai ma Shuhui Sun Yusheng Cai Guibin Wang Jing Qu Weiqi Zhang Si Wang Guang-Hui Liu Advanced Innovation Centerfor Human Brain Protection National Clinical Research Centerfor Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Translational MedicineCenter International Centerfor Aging and CancerBejing Municipal GeriatricMedical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Beijing Institute for Stem Cell and Regenerative Medicine Beijing100101China CAS Key Laboratory of Genomic and Precision Medicine Bejing Institute of Genomics and China National Center for BioinformationChinese Academy of SciencesBeijing 100101China Institute for Stem Cell and Regeneration CASBeijing 100101China The Fifth People's Hospital of Chongqing Chongqing 400062China Sino-Danish Cllege University of Chinese Academy of SciencesBeijing101408China National Laboratory of Biomacromolecules CAS Center for Excelence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing100101China State Key Laboratory of Proteomics Beijing Proteome Research CenterNational Center for Protein Sciences(Beijing)Beijing Institute of LifeomicsBeijing 102206China

Cullin-RING E3 ubiquitin ligases(CRLs),the largest family of multi-subunit E3 ubiquitin ligases in eukaryotic cells,represent core cellular machinery for executing protein degradation and maintaining ***,we asked what roles Cullin proteins play in human mesenchymal stem cell(hMSC)homeostasis and *** this end,we conducted a comparative aging phenotype analysis by individually knocking down Cullin members in three senescence models:replicative senescent hMSCs,Hutchinson-Gilford Progeria Syndrome hMSCs,and Werner syndrome *** all family members,we found that CUL2 deficiency rendered hMSCs the most susceptible to *** investigate CUL2-specific underlying mechanisms,we then applied CRISPR/Cas9-mediated gene editing technology to generate CUL2-deficient human embryonic stem cells(hESCs).When we differentiated these into h MSCs,we found that CUL2 deletion markedly accelerates hMSC ***,we identified that CUL2 targets and promotes ubiquitin proteasome-mediated degradation of TSPYL2(a known negative regulator of proliferation)through the substrate receptor protein APPBP2,which in turn downregulates one of the canonical aging marker-P21^(waf1/cip1),and thereby delays *** work provides important insights into how CRL2^(APPBP2)-mediated TSPYL2 degradation counteracts hMSC senescence,providing a molecular basis for directing intervention strategies against aging and aging-related diseases.

关键词： Cullins stem cell senescence aging proteostasis ubiquitination APPBP2 TSPYL2

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园林用耐候钢的开发

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上海金属 2020年第3期42卷 1-6,11页

作者：王庆芬马到原长春建筑学院建筑与规划学院吉林长春130607 吉林大学材料科学与工程学院吉林长春130012

研制了两种不同成分的园林用耐大气腐蚀钢,其编号为A和B。A钢含0.13%C(质量分数,下同)、0.51%Cr和0.14%Ni,B钢含0.20%C、0.79%Cr和0.60%Ni。A钢的显微组织为条状、块状、针状铁素体和珠光体,B钢的显微组织为珠光体和贝氏体;B钢的强度高... 详细信息

研制了两种不同成分的园林用耐大气腐蚀钢,其编号为A和B。A钢含0.13%C(质量分数,下同)、0.51%Cr和0.14%Ni,B钢含0.20%C、0.79%Cr和0.60%Ni。A钢的显微组织为条状、块状、针状铁素体和珠光体,B钢的显微组织为珠光体和贝氏体;B钢的强度高于A钢,但断后伸长率和0℃冲击吸收能量均低于A钢。开路电位、极化曲线和电化学阻抗谱测试结果表明:铬、镍含量较高的B钢耐腐蚀性能优于A钢,前者的平均腐蚀速率为0.0679 mm/a,后者为0.0870 mm/a,二者的腐蚀产物都主要由α-FeOOH、β-FeOOH、γ-FeOOH和Fe3O4组成,但B钢中Fe3O4更稳定、致密,其保护基体金属的作用更大。

关键词：耐候钢铬镍显微组织力学性能耐蚀性能

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Perioperative toripalimab plus neoadjuvant chemotherapy might improve outcomes in resectable esophageal cancer:an interim analysis of a phase Ⅲ randomized clinical trial

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Cancer Communications 2024年第10期44卷 1214-1227页

作者： Yan Zheng Guanghui Liang Dongfeng Yuan Xianben Liu Yufeng Ba Zimin Qin Sining Shen Zhenxuan Li Haibo Sun Baoxing Liu Quanli Gao Peng Li Zongfei Wang Shilei Liu Jianping Zhu Haoran Wang Haibo ma Zhenzhen Liu Fei Zhao Jun Zhang He Zhang daoyuan Wu Jinrong Qu Jie ma Peng Zhang Wenjie ma Ming Yan Yongkui Yu Qing Li Jiangong Zhang Wenqun Xing Department of Thoracic Surgery The Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouHenanP.R.China Department of Immunotherapy The Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouHenanP.R.China Department of Endoscopy The Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouHenanP.R.China Department of Pathology The Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouHenanP.R.China Department of Radiology The Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouHenanP.R.China Department of Biobank The Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouHenanP.R.China Department of Strategic Development The Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouHenanP.R.China Department of Statistics LinkDoc Technology Co.LtdBeijingP.R.China Department of Cancer Epidemiology The Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouP.R.China

Background:In the era of immunotherapy,neoadjuvant immunochemotherapy(NAIC)for the treatment of locally advanced esophageal squamous cell carcinoma(ESCC)is used clinically but lacks of high-level clinical *** study aimed to compare the safety and long-term efficacy of NAIC followed byminimally invasive esophagectomy(MIE)with those of neoadjuvant chemotherapy(NAC)followed by ***:A prospective,single-center,open-label,randomized phase Ⅲ clinical trial was conducted at Henan Cancer Hospital,Zhengzhou,*** were randomly assigned to receive either neoadjuvant toripalimab(240mg)plus paclitaxel(175 mg/m^(2))+cisplatin(75 mg/m^(2))(toripalimab group)or paclitaxel+cisplatin alone(chemotherapy group)every 3 weeks for 2 *** surgery,the toripalimab group received toripalimab(240 mg every 3 weeks for up to 6 months).The primary endpoint was event-free survival(EFS).The pathological complete response(pCR)and overall survival(OS)were key secondary *** events(AEs)and quality of life were also ***:Between may 15,2020 and August 13,2021,252 ESCC patients ranging fromT1N1-3M0 to T2-3N0-3M0were enrolled for interim analysis,with 127 in the toripalimab group and 125 in the chemotherapy *** 1-year EFS rate was 77.9%in the toripalimab group compared to 64.3%in the chemotherapy group(hazard ratio[HR]=0.62;95%confidence interval[CI]=0.39 to 1.00;P=0.05).The 1-year OS rates were 94.1%and 83.0%in the toripalimab and chemotherapy groups,respectively(HR=0.48;95%CI=0.24 to 0.97;P=0.037).The patients in the toripalimab group had a higher pCR rate(18.6%vs.4.6%;P=0.001).The rates of postoperative Clavien-Dindo grade Ⅲb or higher morbidity were 9.8%in the toripalimab group and 6.8%in the chemotherapy group,with no significant difference observed(P=0.460).The rates of grade 3 or 4 treatment-related AEs did not differ between the two groups(12.5%versus 12.4%).Conclusions:The interim results of this ongoing trial showed that in resec

关键词： esophageal squamous cell carcinoma minimally invasive esophagectomy neoadjuvant chemotherapy neoadjuvant immunochemotherapy survival

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轧制预变形和奥氏体化温度对贝氏体钢显微组织与拉伸性能的影响

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机械工程材料 2023年第7期47卷 62-66,71页

作者：赵秋红王庆芬马到原长春建筑学院管理学院长春130607 吉林大学材料科学与工程学院长春130012

对Fe-0.9C-1.4Si-1.6Mn-0.8Cr-0.2Mo-0.25Al钢锭分别进行冷轧与热轧变形处理,再进行850~1 100℃奥氏体化处理和300℃等温处理,研究了轧制预变形和奥氏体化温度对显微组织和拉伸性能的影响。结果表明:经950℃奥氏体化后,冷轧预变形钢主... 详细信息

对Fe-0.9C-1.4Si-1.6Mn-0.8Cr-0.2Mo-0.25Al钢锭分别进行冷轧与热轧变形处理,再进行850~1 100℃奥氏体化处理和300℃等温处理,研究了轧制预变形和奥氏体化温度对显微组织和拉伸性能的影响。结果表明:经950℃奥氏体化后,冷轧预变形钢主要由贝氏体铁素体和残余奥氏体组成,热轧预变形钢则由贝氏体和残余奥氏体组成,相比于未预变形钢,残余奥氏体含量减少;冷轧变形可同时提高钢的抗拉强度和断后伸长率,热轧变形则会降低断后伸长率。当奥氏体化温度在850~1 050℃时,冷轧预变形钢的残余奥氏体含量变化不大,当奥氏体化温度为1 100℃时,残余奥氏体含量减少并出现片状魏氏体铁素体;随着奥氏体化温度升高,冷轧预变形钢的抗拉强度和断后伸长率先略微增大后减小,当奥氏体化温度为950℃时达到最大。

关键词：贝氏体钢预变形冷轧热轧显微组织拉伸性能

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