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Effect of L-carnitine supplementation during in vitro maturation and in vitro culture on oocyte quality and embryonic development rate of bovines

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Asian pacific Journal of Reproduction 2019年第6期8卷 289-296页

作者： Diego F.Carrillo-González Nélida Rodríguez-Osorio Charles R.Long Neil A.vásquez-Araque Juan G.maldonado-Estrada One Health and veterinary Innovative Research and Development Group School of Veterinary MedicineUniversity of Antioquia050034 MedellinColombia Faculty of Agricultural Sciences School of ZootechnicsUniversidad de SucreColombia Genomics and Bioinformatics Lab Department of Biological SciencesUniversity of the Republic of UruguaySalto CampusUruguay Department of veterinary Physiology and Pharmacology College of Veterinary MedicineTexas A&M UniversityCollege StationTexas 77843-4466USA Grupo de investigación Biotecnología Animal Facultad de CienciasUniversidad Nacional de ColombiaSede MedellínMedellínColombia

Objective:To assess the effect of L-carnitine supplementation during in vitro oocyte maturation and in vitro culture process of bovine ***:L-carnitine(3.8 mm)was added to maturation medium and the effect was assessed in the quality(Experiment 1)and in the cleavage and 4-cells stage(Experiment 2).Besides,the effect of L-carnitine addition on maturation medium(3.8 mm)and culture medium(1.5 mm)on embryo rate production was *** Experiment 1,bovine oocytes from abattoir were randomly separated into two groups(the control group and L-carnitine group)forin vitro *** oocytes were examined for cumulus cells expansion as an indicator of maturation,and the content of the mitochondrial activity,the presence of lipid droplets,the reduced glutathione,and the reactive oxygen species were measured by using specific *** Experiment 2,oocytes were matured as performed in Experiment 1,afterward fertilized and cultured until day 3,and cleavage rate and 4-cells stage rate were *** Experiment 3,in vitro maturation and fertilization were done as performed in Experiment 2,but at day 3 of culture,each group of embryos was separated into two new groups,and L-carnitine(1.5 mm)was added in culture media until day *** cleavage and embryo development rate were determined on the basis with the oocytes put on *** rate was calculated from cleaved ***:The cumulus expansion rate at gradeⅢand mitochondrial activity were significantly higher in the L-carnitine group in comparison with the control group(P0.05).In addition,cleavage and the proportion of embryo development and hatching rate were similar for all groups(P>0.05).Conclusions:L-carnitine as a supplement in culture media improves the cumulus expansion and increases the mitochondrial activity during in vitro maturation process but has no apparent effect on the cleavage and development of bovine *** investigations of L-carnitine addition on in vit

关键词： Antioxidants Culture supplement Embryo development Lipid droplets maturation mitochondria

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CdS nanocrystals as fluorescent probe for detection of dolasetron mesylate in aqueous solution:Application to biomedical analysis

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Journal of Pharmaceutical Analysis 2016年第6期6卷 410-416页

作者： Samadhan P.Pawar Laxman S.Walekar Uttam R.Kondekar Dattatray B.Gunjal Anil H.Gore Prashant v.Anbhule Shivajirao R.Patil Govind B.Kolekar Fluorescence Spectroscopy Research Laboratory Department of ChemistryShivaji University

A simple and straightforward method for the determination of dolasetron mesylate(Dm) in aqueous solution was developed based on the fluorescence quenching of 3-mercaptopropionic acid(mPA) capped Cd S quantum dots(QDs).The structure,morphology,and optical properties of synthesized QDs were characterized by using Uv-vis absorption spectroscopy,fluorescence spectroscopy,transmission electron microscopy(TEm) and dynamic light scattering(DLS) *** the optimum conditions,the mPA-Cd S QDs fluorescence probe offered good sensitivity and selectivity for detecting *** probe provided a highly specific selectivity and a linear detection of Dm in the range of 2–40 μg/m L with detection limit(LOD) 1.512 μg/m *** common excipients did not interfere in the proposed *** fluorescence quenching mechanism of Cd S QDs is also *** developed sensor was applied to the quantification of Dm in urine and human serum sample with satisfactory results.

关键词： Dolasetron mesylate CdS quantum dots Fluorescence quenching Nonradiative recombination

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纯方位跟踪的观察器最佳机动

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情报指挥控制系统与仿真技术 1999年第3期21卷 7-22页

作者： J.m.帕萨鲁克斯 D.v.卡佩尔军仪

在纯方位跟踪中,为确保可观察性和获得精确目标定位,观察器机动极为关键。本文用最佳控制理论来确定匀速观察器的航向,使从费希尔信息矩阵(FIm)导出的精度准则为最小。部分通过解析方法,部分通过数值迭代过程建立和解析出最佳机动的必... 详细信息

在纯方位跟踪中,为确保可观察性和获得精确目标定位,观察器机动极为关键。本文用最佳控制理论来确定匀速观察器的航向,使从费希尔信息矩阵(FIm)导出的精度准则为最小。部分通过解析方法,部分通过数值迭代过程建立和解析出最佳机动的必要条件(欧拉方程)。介绍并讨论了最佳观察器机动的几个实例。

关键词：纯方位跟踪观察器机动目标定位最佳机动

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mRI对妊娠晚期双胎输血综合征子宫内手术后脑损伤的应用价值：一项回顾性队列研究

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国际医学放射学杂志 2021年第4期44卷 495-496页

作者： m.Aertsen C.v.T.De Ten Berghe S.Deneckere I.Couck L.De Catte L.Lewi 李鹏飞

摘要目的由于产前脑损伤的风险增加,对所有接受子宫干预的双胎输血综合征(TTTS)病人提供妊娠晚期 mRI 扫描;但这种策略的效用尚未得到证明,因此需通过 mRI 扫描

关键词：双胎输血综合征妊娠晚期回顾性队列研究子宫内 mRI

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Electron Acceleration via Trapping inside Ion mirror-mode Structures within A Large-scale magnetic Flux Rope

Electron Acceleration via Trapping inside Ion Mirror-mode St...

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2023年中国地球科学联合学术年会

作者： Z.H.Zhong H.Zhang m.Zhou D.B.Graham R.X.Tang X.H.Deng Yu.v.Khotyaintsev

Fermi acceleration is believed as a crucial process for the acceleration of energetic electrons within flux ropes (FRs) during magnetic reconnection. However, in finite-length FRs with a large core field, the finite contracting and the escaping of electrons along the axis can significantly limit the efficiency of Fermi acceleration.

关键词： Electron Acceleration via Trapping inside Ion mirror-mode Structures within A Large-scale magnetic Flux Rope

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Na+ - Ca2+ Exchanger Gene Products. NCX1, NCX2 and NCX3, as Putative Targets for Neuroprotection in Brain Ischemia

Na+ - Ca2+ Exchanger Gene Products. NCX1, NCX2 and NCX3, as ...

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第十五届国际药理学大会

作者： Annunziato L Pignataro G Sirabella R Saggese m Cuomo O Gala R Sec-ondo A viggiano D Boscia F molinaro P valsecchi v Tortiglione A Di Renzo GF Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly

＜正＞ NCX is a neuronal plasmamembrane antiporter, which, by coupling Ca2 + and Na+ fluxes, plays a relevant role in brain ischemia. In the CNS there are 3 different NCX gene products NCX1, NCX2, and NCX3. NCX transcript and protein expression is differently regulated after ischemia in the focal region and in the peri-infarct area. The pharmacological inhibition of NCX activity is detrimental in the development of ischemic damage. NCX knocking out through antisense strategy showed that NCX1 and NCX3 play a major role in NCX neuroprotective action. Accordingly. NCX3 transfected BHK cells are less vulnerable to chemical hypoxia. Interestingly, the expression of NCX1 and NCX3 is up-regulated by NGF in PC12 cells. This modulation occurs via two pathways activated by tyro-sine kinase receptors. Ras-Raf-mek-mAPK pathway and PI-3K/AKT pathway. In fact, the mek inhibitor PD 98059 or the PI-3K inhibitor LY 294002 prevented this up-regulation. whereas AKT-1 PC12 cell positive mutants showed an increase in NCX1 and NCX3 expression. These data demonstrate that NCX products display a differential expression in the development of ischemic damage and can be selectively upregulated through NGF-mediated trasductional pathways

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Patterns of Gustatory Recovery in Patients Affected by the COvID-19 Outbreak

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virologica Sinica 2020年第6期35卷 833-837页

作者： Carlos m.Chiesa-Estomba Jerome R.Lechien maria R.Barillari Sven Saussez COvID-19 Task Force of the Young-Otolaryngologists of the International Federations of Oto-rhino-laryngological Societies(YO-IFOS) ParisFrance Department of Otorhinolaryngology-Head and Neck Surgery OsakidetzaHospital Universitario DonostiaSan Sebastian 20014Spain Biodonostia Health Research Institute San Sebastian 20014Spain Department of Human Anatomy and Experimental Oncology Faculty of MedicineUMONS Research Institute for Health Sciences and TechnologyUniversity of Mons(UMons)7000 MonsBelgium Department of Otolaryngology-Head and Neck Surgery Foch HospitalSchool of MedicineUFR Simone VeilUniversite´Versailles Saint-Quentin-en-Yvelines(Paris Saclay University)Paris 92150France Department of Otorhinolaryngology and Head and Neck Surgery CHU de BruxellesCHU Saint-PierreSchool of MedicineUniversite´Libre de BruxellesBrussels 1000Belgium Department of mental and Physical Health and Preventive medicine "L.Vanvitelli"University80138 NaplesItaly Department of Head and Neck Surgery EpiCURA HospitalHornu 7331Belgium

Dear Editor,Coronavirus disease 2019(COvID-19)is a viral infection caused by severe acute respiratory syndrome coronavirus 2(SARS-Co v-2).From march 2020,several studies indicate that many subjects affected by mild-to-moderate COvID-19 presented olfactory/gustatory dysfunction(OD/GD)that appeared strongly correlated between them but not with the other symptoms suggestive of upper airway infection(Lechien et al.2020a,b;Hopkins et al.2020;Paderno et al.2020).

关键词： Hopkins Pattern

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mX导弹喷管用先进材料评论

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固体火箭技术 1978年第2期 101-110页

作者： W、v、Kotlensky ,T、G、Flock ,D、m、Rogers ,H、O、Davis ,R、A、Ellis ,刘宝芬

目前正在进行研制石墨材料,准备用于mX 第一级和第二级固体火箭喷管的入口部,喉部,后喉部,出口锥和可延伸出口锥。这些材料必须经受住新型洲际弹道导弹（ICBm）喷管系统的苛刻的结构、化学侵蚀,粒子冲刷和高温环境。该系统的燃烧室压力... 详细信息

目前正在进行研制石墨材料,准备用于mX 第一级和第二级固体火箭喷管的入口部,喉部,后喉部,出口锥和可延伸出口锥。这些材料必须经受住新型洲际弹道导弹（ICBm）喷管系统的苛刻的结构、化学侵蚀,粒子冲刷和高温环境。该系统的燃烧室压力可达127公斤/厘米2,火焰温度为3700°K 至3922°K。叙述了第一级和第二级全尺寸试验喷管的现状。还提出了在喷管设计、石墨材料研制以及预期性能改进方面进一步工作的方向。

关键词：碳/碳复合材料出口锥第一级第二级热解石墨炭/炭复合材料碳纤维增强复合材料可延伸喷管全尺寸发动机酚醛复合材料三向编织烧蚀石墨材料导弹系统 mX

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validation of glaucoma-like features in the rat episcleral vein cauterization model

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Chinese medical Journal 2014年第2期127卷 359-364页

作者： Bai Yujing Zhu Yingting Chen Qin Xu Jing marinko v. Sarunic Uri H. Saragovi Zhuo Yehong Key Laboratory of vision Loss and Restoration Ministry of Education Department of Ophthalmology Peking University People's Hospital Beijing 100044 China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou Guangdong 510060 China Department of Ophthalmology First'Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China Engineering Science Simon Fraser University Burnaby BC Canada V5A 1S6 Department of Pharmacology and Therapeutics Department of Oncology and the Cancer Center Bloomfield Centre for Research in Aging Lady Davis Institute-Jewish General Hospital McGill University Montreal Quebec Canada H3T 1E2

Background Glaucoma,an irreversible optic nerve neuropathy,always results in *** study aimed to evaluate glaucoma-like features in the rat episcleral vein cauterization （EvC） model by multiple in vivo and in vitro *** Wistar rat was used in this *** elevated intraocular pressure （IOP） was induced by cauterization of three episcleral *** was monitored with Tono-Pen XL ***-dependent changes to the neuronal retinal layers were quantified by Fourier domain-optical coherence *** function of retina was evaluated by electroretinogram （ERG）.Survival of retinal ganglion cells （RGCs） was quantified by retrograde *** study was performed with retinal sections stained with hematoxylin-eosin,glial fibrillary acidic protein,and neuronal nuclear *** and aqueous humor protein were extracted and cytotoxic protein tumor necrosis factor alpha （TNF-α） and alpha-2 macroglobulin （α2m） were measured with Western *** EvC is a relatively facile intervention,with low failure rates （＜5％）.After surgical intervention,chronic mild lOP elevation （about 1.6-fold over normal,P ＜0.05） was induced for at least 6 weeks without requiring a second *** lOP causes chronic and progressive loss of RGCs （averaging about 4％ per week）,progressive thinning of neuronal retinal layers （3-5 μm per week）,and reduction of a-and b-wave in *** method can also induce glial cell activation and alterations of inflammation proteins,such as TNF-α and α*** EvC method can establish a robust,reliable,economic and highly reproducible glaucomatous animal model.

关键词： glaucoma experimental animal model retinal neurons rat

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国际机器理论与机构学联合会委员会A“名词术语标准”——机器理论与机构学名词术语

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机械设计与研究 1985年第6期 79-126页

作者： G.BOGELSACK ,F.J.GIERSE ,v.ORAvSKY ,J.m.PRENTIS ,A.ROSSI

国际机器理论与机构学联合会名词术语标准委员会编写的机器理论与机构学名词术语,已在国际机械学界有一定影响,并陆续出版了几个不同语种的版本。该标准对我国机械学界具有参考价值。为了有利于国内外的学术交流,本刊特将此全文刊载于... 详细信息

国际机器理论与机构学联合会名词术语标准委员会编写的机器理论与机构学名词术语,已在国际机械学界有一定影响,并陆续出版了几个不同语种的版本。该标准对我国机械学界具有参考价值。为了有利于国内外的学术交流,本刊特将此全文刊载于本期。1986年拟发行单行本,如需要的单位或个人可来函,与上海交通大学出版社发行科联系。

关键词：名词术语标准机构学运动副元素构件变化率机械元件运动倒置刚体运动等于零广义坐标齿轮机器委员会

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