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Analyzing the genomic and transcriptomic architecture of milk traits in murciano-Granadina goats

Journal of Animal Science and Biotechnology 2020年第3期11卷 649-666页

作者： Dailu Guan Vincenzo Landi maría Gracia Luigi-Sierra Juan Vicente Delgado Xavier Such Anna Castelló Betlem Cabrera Emilio mármol-Sánchez Javier Fernández-Alvarez JoséLuis Ruiz de la Torre Casañas Amparo martínez Jordi Jordana marcel Amills Centre for Research in Agricultural Genomics(CRAG) CSIC-IRTA-UAB-UBUniversitat Autònoma de Barcelona08193 BellaterraSpain Departamento de Genética Universidad de Córdoba14071 CórdobaSpain Departament de Ciència Animal i dels Aliments Facultat de VeterinàriaUniversitat Autònoma de Barcelona08193 BellaterraSpain Asociación Nacional de Criadores de Caprino de Raza murciano-Granadina(CAPRIGRAN) 18340 GranadaSpain Servei de Granges i Camps Experimentals Universitat Autònoma de Barcelona08193 BellaterraSpain

Background:In this study,we aimed to investigate the molecular basis of lactation as well as to identify the genetic factors that influence milk yield and composition in *** achieve these two goals,we have analyzed how the mRNA profile of the mammary gland changes in seven murciano-Granadina goats at each of three different time points,i.e.78 d(T1,early lactation),216 d(T2,late lactation)and 285 d(T3,dry period)after ***,we have performed a genome-wide association study(GWAS)for seven dairy traits recorded in the 1st lactation of 822 murciano-Granadina ***:The expression profiles of the mammary gland in the early(T1)and late(T2)lactation were quite similar(42 differentially expressed genes),while strong transcriptomic differences(more than one thousand differentially expressed genes)were observed between the lactating(T1/T2)and non-lactating(T3)mammary glands.A large number of differentially expressed genes were involved in pathways related with the biosynthesis of amino acids,cholesterol,triglycerides and steroids as well as with glycerophospholipid metabolism,adipocytokine signaling,lipid binding,regulation of ion transmembrane transport,calcium ion binding,metalloendopeptidase activity and complement and coagulation *** regard to the second goal of the study,the performance of the GWAS allowed us to detect 24 quantitative trait loci(QTLs),including three genome-wide significant associations:QTL1(chromosome 2,130.72-131.01 mb)for lactose percentage,QTL6(chromosome 6,78.90-93.48 mb)for protein percentage and QTL17(chromosome 17,11.20 mb)for both protein and dry matter ***,QTL6 shows positional coincidence with the casein genes,which encode 80%of milk ***:The abrogation of lactation involves dramatic changes in the expression of genes participating in a broad array of physiological processes such as protein,lipid and carbohydrate metabolism,calcium homeostasis,cell death and tissue remo

关键词： Casein genes Dairy traits GWAS Lactation QTLs RNA-Seq

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Chemical Exposure Hazardous for Fish Hyphessobrycon eques through the Incorrect Release of Oil in Amazon Region

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Journal of Fisheries Science 2020年第2期2卷 19-23页

作者： Natalino da Costa Sousa Arthur dos Santos da Silva Keber Santos Costa Junior Francisco Alex Lima Barros Geisy Correa de Oliveira Pedro Rodrigo Nery de Souza Claudmilson Rodrigues de mesquita Bruna de jesus Santos Alexander Damaceno Lima santos Winnícius muniz dos Santos Sá Nayara Siqueira dos Santos márcia Valéria Silva do Couto Federal University of Pará(UFPA) Campus BragançaAlameda Leandro RibeiroBragança68600-000PABrazil Federal University of Pará(UFPA) Campus de TucuruíRua Itaipu36-Vila PermanenteTucuruí68464-000PABrazil Federal University of de Sergipe(UFS) Av.Marechal Rondons/n-Jardim Rosa ElzeSão Cristóvão49100-000SEBrasil Faculdade Pio Décimo Av.Pres.Tancredo Neves 5655-Jabotiana49095-000Aracaju–SE.Brazil

Incorrect discard of oil used by fishing ship in the brazilian north region has become a common *** the possible hazard to the aquatic organisms,still missing scientific data about their ***,this study aimed to evaluate the stress and lethality caused by lubricant oil(FSAOLU)on fish Hyphessobrycon ***,it was used six different concentration diluted in water(0,22,24,26,28,30 and 32%of oil)and three replaces during 96 *** the end or during the experiment(with dying fish),it was collected blood samples(cutting the caudal fin)to determine physiological *** FSAOLU showed mean lethal dose(LD50-96h)of 27.36%,classified as toxic causing alterations in glucose values from the 26%.The greater dilution of FSAOLU(32%)increased 115%in glucose values when compared to the ***,lubricant oil when discarded in water,it present hazard to the aquatic organisms causing stress and mortality for fish being necessary adequate management to discard of this residue.

关键词： Toxicity FSA Sensibility Stress

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重症肌无力治疗性单价IgG抗体基因的构建

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中国免疫学杂志 2009年第11期25卷 1003-1005页

作者：李钟燮孟繁平孙昌元李芳芳金权鑫李红花李英信 m.Stassen m.de Baets 延边大学基础医学院免疫学与病原生物学教研部延吉133000 Department of Neurology Faculty of MedicineUniversity of Maastricht

目的:构建一株对重症肌无力(mG)具有特异性免疫治疗作用的单价IgG类抗体基因。方法:应用定点突变技术,将致病性抗乙酰胆碱受体(AChR)抗体IgG637的重(H)链第322位氨基酸进行K322A突变,获得的突变型抗体IgG637/K322A基因再进行H链互补决定... 详细信息

目的:构建一株对重症肌无力(mG)具有特异性免疫治疗作用的单价IgG类抗体基因。方法:应用定点突变技术,将致病性抗乙酰胆碱受体(AChR)抗体IgG637的重(H)链第322位氨基酸进行K322A突变,获得的突变型抗体IgG637/K322A基因再进行H链互补决定区3(CDR3)缺失突变,获得突变型抗体IgG637/K322A/CDR3ΔPLKP基因。经转化大肠杆菌XL1-Blue进行增殖后,转染哺乳类细胞CHO-k1进行表达,表达产物经ELIAS检测与补体C3的结合活性,经ria检测与特异性抗原人AChR的结合活性。突变抗体H链再经杵(T366Y)臼(Y407T)突变,以利于异源H链的配对。结果:突变型抗体IgG637/K322A丧失了与补体C3结合的能力,突变型抗体IgG637/K322A/CDR3ΔPLKP丧失了与人AChR结合的能力。测序证实已经获得了预想的杵臼突变序列。结论:已经成功的制备了无补体激活能力的单价IgG类抗AChR抗体基因。

关键词：重症肌无力单价抗体定点突变免疫球蛋白G

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Tumor heterogeneity and therapy resistance-implications for future treatments of prostate cancer

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Journal of Cancer metastasis and Treatment 2017年第1期3卷 302-314页

作者： Fiona m.Frame Amanda R.Noble Sandra Klein Hannah F.Walker Rakesh Suman Richard Kasprowicz Vin m.mann matt S.Simms Norman J.maitland Cancer Research Unit Department of BiologyUniversity of YorkHeslingtonNorth Yorkshire YO105DDUK Black Family Stem Cell Institute Icahn School of Medicine at Mount SinaiNew YorkNY 10029USA Phase Focus Limited Electric WorksSheffield Digital CampusSheffield S12BJUK Department of Urology Castle Hill Hospital(Hull and East Yorkshire Hospitals NHS Trust)Cottingham HU165JQUK Hull York medical School University of HullHull HU67RXUK.

Aim:To develop new therapies for prostate cancer,disease heterogeneity must be *** includes patient variation,multi-focal disease,cellular heterogeneity,genomic changes and epigenetic *** requires more representative models to be used in more innovative ***:This study used a panel of cell lines and primary prostate epithelial cell cultures derived from patient *** assays were used;alamar blue,colony forming assays,γH2AX and Ki67 immunofluorescence and comet *** quantitative phase imaging(QPI),a label-free imaging technique,combined with Cell Analysis Toolbox software,was implemented to carry out real-time analysis of cells and to retrieve morphological,kinetic and population ***:A combination of radiation and Vorinostat may be more effective than radiation *** prostate cancer stem-like cells are more resistant to etoposide than more differentiated *** of QPI images showed that cell lines and primary cells differ in their size,motility and proliferation rate.A QPI signature was developed in order to identify two subpopulations of cells within a heterogeneous primary ***:Use of primary prostate epithelial cultures allows assessment of therapies whilst taking into account cellular *** of rare cell populations and embracing novel techniques may ultimately lead to identifying and overcoming treatment resistance.

关键词： Prostate ptychography live-cell imaging primary cells quantitative phase imaging

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Cephalometric norms for a sample of Emirates adults

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Open Journal of Stomatology 2011年第3期1卷 75-83页

作者： Huda m. Abu-Tayyem Amna H. Alshamsi Sayed Hafez Eman mohie ElDin 不详

Introduction—European-American norms are still used in the orthodontic treatment of Emirates patients despite the different ethnic backgrounds of the Emirates. The purpose of this study was to formulate cephalometric norms for lateral cephalometric mea-surements of Emirates adults and to study gender differences. methods—Lateral cephalometric radio-graphs of 176 Emirates adults, 91 males and 85 fe-males, were selected according to the following crite-ria;an age range between 19 to 25 years, esthetically pleasing and harmonious faces with balanced and acceptable profiles and occlusions, normal skeletal and dental relationship, all permanent teeth present, and no history of orthodontic treatment or facial trauma. The mean values and standard deviations of 91 angular and 21 linear cephalometric variables were calculated using the Dolphin version 10.5 soft-ware package. The resulting norms for Emirates male and female groups were compared using a student t-test. Results—Several statistically significant gender differences were noticeable. Skeletally, Emirates males showed significantly greater (p m (°),and FH/mP (°) angles than females. In addition, Emirates males had significantly greater (p mm), Co-Gn (mm), and significantly greater (p mm) than females. Dentally, Emirates males had significantly greater (p mm), and L1-NB (mm) (p males. For soft tissue measurements there were significant gender differences (p measurements except for lower lip to E-plane and nose prominence measurements which have no significant gender difference. Emirates males revealed greater measurements than females except for soft tissue con-vexity, Z-angle, and both upper and lower lips pro-trusion which were significantly greater in Emirates females.

关键词： Emirates Norms Cephalometric Norms

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血清骨唾液酸蛋白的生物稳定性及在肝、肾功能衰竭患者中的变化

血清骨唾液酸蛋白的生物稳定性及在肝、肾功能衰竭患者中的变化

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全国老年骨质疏松专题学术研讨会

作者：李裕明 m.J.Seibel R.Ziegler 武汉同济医科大学附属和医院内分泌科德国海德堡大学医学院内科Ⅰ医院

将12个正常人分成4组,每组采血200ml并提取血清,分别观察血清骨唾液酸蛋白(bonesialoprotein,BSP)在4℃、20℃、28℃、37℃和-30℃条件下放置不同时间后血清BSP浓度的变化情况及多次解冻对血清BSP浓度影响.观察30例肾功能衰竭及22例肝... 详细信息

将12个正常人分成4组,每组采血200ml并提取血清,分别观察血清骨唾液酸蛋白(bonesialoprotein,BSP)在4℃、20℃、28℃、37℃和-30℃条件下放置不同时间后血清BSP浓度的变化情况及多次解冻对血清BSP浓度影响.观察30例肾功能衰竭及22例肝功能衰竭的血清BSP浓度变化.血清BSP测定采用ria法.结果发现,血清BSP在4℃条件下放置4h后,其免疫活性浓度明显升高,在20℃以上条件下放置2h以上,其浓度明显升高,在-30℃条件下放置4周后,血清BSP浓度明显升高,多次解冻对血清BSP影响不明显.在肾功能衰竭患者,血清BSP浓度明显升高,已与尿肌酐清除率比较呈明显负相关性(r=-0.67,P<0.001).在肝功能衰竭患者,血清BSP浓度亦明显升高,但与相应的肝功能比较无相关性改变.提示,外界温度对血清BSP浓度有明显影响.在肝、肾功能衰竭患者,血清BSP是一个有效的骨代谢参数,并可能作为一个观察骨代谢紊乱的常规指标.

关键词：骨唾液酸蛋白稳定性

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Virulence Factors in methicillin-Resistant Staphylococcus aureus Isolated from ICU Units in Brazil

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Advances in microbiology 2014年第4期4卷 207-215页

作者： Simone G.Souza Guilherme B.Campos Pollianna S.Oliveira Daniel S.Sousa Danilo C.C.Da Silva Verena m.Santos Aline T.Amorim Angelita m.O.G.Santos Jorge Timenetsky mariluze P.Cruz Regiane Yatsuda Lucas m.marques Instituto multidisciplinar em Saúde Núcleo de Tecnologia em SaúdeUniversidade Federal da BahiaVitória da ConquistaBrazil Instituto de Ciências Biomédicas Departamento de MicrobiologiaUniversidade de Sao PauloSao PauloBrazil

Species of Staphylococcus are common in hospital infection (HI). methicillin resistant S. aureus (mRSA) has also become a serious problem in Brazilian HI. The aim of this study was to characterize the pathogenicity of methicillin-resistant S. aureus (mRSA) and methicillin-sensitive Staphylococcus aureus (mSSA) isolated in public hospitals. The clinical isolates were obtained from intensive care unit. The mRSA and mSSA strains were genotyped by PCR for detection genes related to virulence factors. moreover, the strains were tested for biofilm formation and cytokine induction in macrophages. Three strains of mRSA (9.68%) expressed the Sea gene, one (3.23%) Seb, 17 (54.84%) Spa and seven (22.58%) had PVL. Two mSSA strains (2.98%) expressed the Sea gene, three (4.48%) Seb, 18 (26.87%) Spa and 11 (16.42%) showed positive results for the PVL gene. There was no expression of Sec and CflA between mRSA and mSSA strains. Among mRSA and mSSA isolates, none statistical differences were observed in biofilm production. The analysis of cytokine induction in the inflammatory response of J774 macrophages by mRSA and mSSA isolates did not show statistical difference. Understanding the mechanisms of pathogenesis of S. aureus could provide important clues for both preventing and treating infection caused by these organisms.

关键词： mRSA mSSA Virulence Factor Hospitalar Infection

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关于西阿翼龙系统位置的简单注释及与其它翼龙类关系的评论(英文)

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地球学报 2010年第S1期31卷 73-75页

作者： Bruno C.VILA NOVA Alexander W.A.KELLNER Juliana m.SAYAO Departamento de Geologia Laboratório de Geologia Sedimentar e Ambiental-Universidade Federal de Pernambuco.RecifeBrazil Paleovertebrate Sector Department of Geology and PaleontologyMuseu Nacional/UFRJRio de JaneiroBrazil Núcleo de Biologia Centro Acadêmico de VitóriaUniversidade Federal de Pernambuco.Vitória de Santo AntaoBrazil

Cearadactylus atrox is one of the first pterosaurs unearthed from the Araripe Basin (Northeast Brazil) (Leonardi and Borgomanero 1983, 1985) (Fig.1).

关键词：翼龙系统位置类关系注释评论简单

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Expression of multidrug Resistance ATP-Binding Cassette(ABC)Transporters in Canine mammary Tumors

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Advances in Breast Cancer Research 2015年第3期4卷 77-85页

作者： Breno S.Salgado Suely Nonogaki Luisa m.Soares Angela Akamatsu Cristiano R.N.da Silva Thiago P.Anacleto Rodolfo malagó Rafael m.Rocha Fátima Gartner Noeme S.Rocha Departamento de Patologia Faculdade de Medicina de BotucatuUniversidade Estadual PaulistaCampus de BotucatuBotucatuBrazil Instituto de Ciências Biomédicas Abel Salazar Universidade do Porto(Intitute of Biomedical Sciences Abel Salazar of University of Porto)OportoPortugal Fundacao Antonio Prudente Hospital A.C.CamargoSao PauloBrazil Departamento de Clínica Veterinária Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual PaulistaUNESP.BotucatuBrazil Hospital Escola de medicina Veterinária Fundacao de Ensino e Pesquisa de Itajubá—FEPIItajubáBrazil

mammary neoplasms are the most common tumors in female dogs. They are usually treated using solely surgical mastectomy—which is recognized as unsatisfactory in many cases. Given this, the benefits of chemotherapy in dogs with mammary cancer need to be further explored. Some drugs that can be used for treating canines with mammary tumors may be substrates of uptake and/or efflux transporters such as the ATP-binding cassette (ABC) transporters. Unfortunately, very little is known regarding the pathobiology of such proteins in canine tumors, including mammary cancer. Accordingly, this study was designed to characterize the expression of ABC transporters P-glycoprotein, mRP1, and mRP2 and their relation with clinicopathologic factors in order to allow a better understanding of their influence in canine mammary cancer. P-glycoprotein was expressed in tumors from 55.8% of patients, while mRP1 and mRP2 were expressed in 37.2% and 39.5% of tumors, respectively. P-glycoprotein expression showed to be related with regional lymph node spread (P = 0.0038), as well as with tumor grade (P = 0.0353) and with a shorter survival (P = 0.0245). mRP1 revealed a strong association with a higher histological grade (P mRP1 was determined as prognostic indicator independent of lymph node status using Cox proportional-hazards regression multivariate analysis (P = 0.0216). No relations between mRP2 and clinicopathologic features were observed. We have found that P-glycoprotein and mRP1 are expressed in highly aggressive canine mammary tumors and are related with poor prognosis. Our results suggest that they may play a significant role in the course of canine mammary cancer progression and be promising candidate markers for a validation study on therapy outcome.

关键词： mDR1 P-Gp mRP1 mRP2 ABCC2 mammary Neoplasms Prognosis Dog

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From Helicobacter pylori infection to gastric cancer:Current evidence on the immune response

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World Journal of Clinical Oncology 2022年第3期13卷 186-199页

作者： Vinícius Lima de Souza Gonçalves maria Luísa Cordeiro Santos marcel Silva Luz Hanna Santos marques Breno Bittencourt de Brito Filipe Antônio França da Silva Cláudio Lima Souza márcio Vasconcelos Oliveira Fabrício Freire de melo Universidade Estadual do Sudoeste da Bahia Campus Vitória da ConquistaVitória da Conquista 45083-900BahiaBrazil Universidade Federal da Bahia Instituto Multidisciplinar em SaúdeVitória da Conquista 45029-094BahiaBrazil

Gastric cancer(GC)is the result of a multifactorial process whose main components are infection by Helicobacter pylori(***),bacterial virulence factors,host immune response and environmental *** development of the neoplastic microenvironment also depends on genetic and epigenetic changes in oncogenes and tumor suppressor genes,which results in deregulation of cell signaling pathways and apoptosis *** review summarizes the main aspects of the pathogenesis of GC and the immune response involved in chronic inflammation generated by ***.

关键词： Gastric cancer Helicobacter pylori Chronic inflammation Host immune response

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