Tumor heterogeneity and therapy resistance-implications for future treatments of prostate cancer
Tumor heterogeneity and therapy resistance -implications for future treatments of prostate cancer作者机构：Cancer Research UnitDepartment of BiologyUniversity of YorkHeslingtonNorth Yorkshire YO105DDUK Black Family Stem Cell InstituteIcahn School of Medicine at Mount SinaiNew YorkNY 10029USA Phase Focus LimitedElectric WorksSheffield Digital CampusSheffield S12BJUK Department of UrologyCastle Hill Hospital(Hull and East Yorkshire Hospitals NHS Trust)Cottingham HU165JQUK Hull York Medical SchoolUniversity of HullHull HU67RXUK.
出 版 物：《Journal of Cancer Metastasis and Treatment》 (癌症转移与治疗（英文版）)
年 卷 期：2017年第3卷第1期
页 面：302-314页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：funded by a PCUK Innovation Award-RIA15-ST2-022.SK was supported by a White Rose Fund studentship
主 题：Prostate ptychography live-cell imaging primary cells quantitative phase imaging
摘 要：Aim:To develop new therapies for prostate cancer,disease heterogeneity must be *** includes patient variation,multi-focal disease,cellular heterogeneity,genomic changes and epigenetic *** requires more representative models to be used in more innovative ***:This study used a panel of cell lines and primary prostate epithelial cell cultures derived from patient *** assays were used;alamar blue,colony forming assays,γH2AX and Ki67 immunofluorescence and comet *** quantitative phase imaging(QPI),a label-free imaging technique,combined with Cell Analysis Toolbox software,was implemented to carry out real-time analysis of cells and to retrieve morphological,kinetic and population ***:A combination of radiation and Vorinostat may be more effective than radiation *** prostate cancer stem-like cells are more resistant to etoposide than more differentiated *** of QPI images showed that cell lines and primary cells differ in their size,motility and proliferation rate.A QPI signature was developed in order to identify two subpopulations of cells within a heterogeneous primary ***:Use of primary prostate epithelial cultures allows assessment of therapies whilst taking into account cellular *** of rare cell populations and embracing novel techniques may ultimately lead to identifying and overcoming treatment resistance.
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