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Dose-dependent effect of histamine on antibody generation in vivo

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Asian Pacific Journal of Tropical medicine 2010年第2期3卷 112-116页

作者： Tripathi T Shahid m Khan hm Khan RA Siddiqui mU DePartment of Biochemistry Faculty of MedicineJawaharlal Nehru Medical College and HospitalAligarh Muslim University Department of microbiology Faculty of MedicineJawaharlal Nehru Medical College and HospitalAligarh Muslim University Department of Pharmacology Faculty of MedicineJawaharlal Nehru Medical College and HospitalAligarh Muslim University Department of Biochemistry Faculty of MedicineJawaharlal Nehru Medical College and HospitalAligarh Muslim University

Objective:To delineate immunomodulatory role of histamine on antibody generation pr of ile in rabbit in the present dose-dependent histamine ***:The cohort comprised of three groups(Ⅲ,ⅣandⅤ),containing six rabbits each,and received subcutaneous histamine 50μg/kg×bis in die(b.i.d.),100μg/kg×*** 200μg/kg X b.i.d.,respectively for 10 days (starting from the 1st day).They were subsequently immunized on the 3rd day with intravenous injection of sheep blood cell(SRBC)(1×10 cells/mL).GroupⅡ(positive control)(n=6) received vehicle(sterile distilled water) and immunized at day 3 similarly while groupⅠ(negative control) (n=6) remained non-immunized and received only *** experimentations were performed in *** samples were collected on pre-immunization(pre-I)(day 0),as well as on days 7-,14-,21-,28- and 58- post-immunization(post-I).Immunological parameters[total immunoglobulins(Igs),Igm and IgG]were analyzed by enzyme linked immunosorbent assay (ELISA) ***:Histamine could influence a detectable antibody response to SRBC as early as day 7-post-I,which lasted until day 58- *** results were found statistically significant(Pmine has a short-term effect on antibody generation(until its presence in the body),and the antibody generation titer in vivo were affected by the concentration of histamine.

关键词： Histamine Immumomodulation T-cell dependent antibody response Humoral immune response SRBC

COX-2 polymorphisms-765G→C and-1195A→G and colorectal cancer risk

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World Journal of Gastroenterology 2009年第36期15卷 4561-4565页

作者： Juliёt H Hoff Rene hm te morsche Hennie mJ Roelofs Elise mJ van der Logt Fokko m Nagengast Wilbert hm Peters Department of Gastroenterology Radboud University Nijmegen Medical Center6500 HB NijmegenThe Netherlands

AIm： TO determine the possible modulating effect of the COX-2 polymorphisms, -765G→C and -1195A→G, on the risk of colorectal cancer （CRC） in a Dutch population. mETHODS： This case-control study includes 326 patients with CRC and 369 age- and gender-matched controls. Genotypes of the COX-2 polymorphisms -7dEG→C and -1195A→G were determined by polymerase chain reaction-based restriction fragment length polymorphism. COX-2 genotypes and haplotypes were analyzed and odds ratios with 95% confidence intervals were estimated by logistic regression. RESULTS： The -765GG genotype was associated with an increased risk of developing CRC （OR, 1.45; 95% CI, 1.03-2.04）. No significant difference was observed in the genotype distribution of the -1195A→G polymorphism between patients and controls. The GG/AC haplotype was present significantly less often in patients than in controls （OR 0.44; 95% CI, 0.22-0.85）. When the AC, AG and GG haplotypes were investigated separately, the AC haplotype showed a tendency to be less frequent in patients than in controls （OR（AG/AC） 0.78; 95% CI, 0.57-1.06）. CONCLUSION： The -765GG genotype is associated with an increased risk of developing CRC and the G6/ AC haplotype seems to protect against CRC. These findings suggest a modulating role for the COX-2 polymorphisms -765G→C and -1195A→G in the development of CRC in a Dutch population.

关键词： Colorectal carcinoma Cyclooxygenase-2 Genetic polymorphism

A Whitecap Coverage model Based on the Surface Slope For Criterion of Wave Breaking

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China Ocean Engineering 1998年第1期13卷 73-85页

作者： Xu, DL Yu, DY Lu, hm Ocean Univ Qindao Phys Oceanog Lab Qingdao 266003 Peoples R China Ocean Univ Qindao Sch Engn Qingdao 266003 Peoples R China

Using the limit surface slope as a criterion of wave breaking, a simple model for estimating the spatial fraction of breaking surface of sea at an instant, which is regarded as the whitecap coverge in this paper, is analytically derived from the probability density of surface slope based on Gaussian statistics. The resulting fraction is found depending on the fourth moment of wave spectum, m(4), as well as the critical threshold of surface slope. By expressing the fourth moment in terms of the Neumann spectrum, a formula linking the fraction and wind speed for fully developed sea states is obtianed. Another formula relating the fraction to both wind speed and fetch (or duration) is achieved by expressing m, in terms of the Krylov spectrum and applying the empirical relationships used in the SmB ocean wave predicting technique. A comparison between these results and the field data of whitecap coverage collected by monahan and O'muircheartuigh shows an encouraging agreement.

关键词： whitecap coverage criterion of wave breaking wind speed wind fetch wind duration fully developed sea state fetch-limited sea state

DFT investigation of half-metallic ferromagnetic rare earth based spinels mgHo_(2)Z_(4)(Z=S,Se)

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Journal of Rare Earths 2024年第1期42卷 121-128,I0005页

作者： maiza Zanib mumtaz manzoor N.A.Noor m.Waqas Iqbal mazia Asghar H.H.Hegazy A.Laref Department of Physics Riphah International UniversityLahore CampusPakistan Research Centre for Advanced materials Science King Khalid UniversityP.O.Box 9004AbhaSaudi Arabia Department of Physics Faculty of ScienceKing Khalid UniversityP.O.Box 9004AbhaSaudi Arabia Department of Physics and Astronomy College of ScienceKing Saud UniversityRiyadh11451Saudi Arabia

Half-metallic ferromagnetism,mechanical as well as thermoelectric properties for rare earth-based spinels mgHo_(2)Z_(4)(Z=S,Se)were investigated using density functional theory(DFT).Structural optimization was done with Perdew-Burke-Ehrenzorf(PBE)sol-generalized gradient approximation(GGA)to calculate the lattice constant of both spinels comparable to experimental *** addition,Born stability criteria and negative formation energy show that our studied spinels are also structurally and dynamically stable in the cubic *** ferromagnetic(Fm)state stability,we also calculated the energy differences among Fm,antiferromagnetic(AFm),and non-magnetic(Nm)***,Curie temperatures of ferromagnetic phases were also *** used Trans-Blaha improved BeckeJohnson(TB-mBJ)potential functional for electronics as well as magnetic characteristics,which lead to the consistent explanation of half-metallic ferromagnetism,representing the whole band-occupancy in material with exact detail of density of states(DOS).The stable Fm state was examined in spinels due to the exchange splitting of Ho cation consisting of p-d hybridizations compatible with the result achieved for electronics band structure and ***,spin magnetic moment was explained in terms of anion,cation,and sharing charge on studied *** addition,the calculated thermoelectric properties clearly show that operation range of these systems may be utilized by future experimental works for identifying the potential applications of these systems.

关键词： Ho-based spinels DFT simulations mechanical parameters Electronic properties hm ferromagnetism Figure of merit

Evaluation of diameter dis-tribution of inside cavities of open CNTs by analyses of nitrogen cryo-adsorption isotherm

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Chinese Science Bulletin 2001年第15期46卷 1317-1320页

作者： Yang, QH Li, F Hou, PX Liu, C Liu, m Cheng, hm Chinese Acad Sci Inst Met Res Shenyang 110015 Peoples R China

Precise evaluation of innerdiameter distribution of open carbon nanotubes (CNTs) is the basis of systematic investigation of physico-chemical processes occurring in nano-sized quasi-1-dimensional carbons. Due to the porosity characteristics and adsorption properties, this study evaluated the innerdiameter and its distribution of carbon nanotubes by analyses of nitrogen cryo-adsorption isotherms, and proved that the gas adsorption method is an effective method to characterize the inner cavity structure in comparison with that of electron microscopy observations and Raman measurements. The advantages of this method are as follows: Firstly, statistical information for innerdiameter distribution of open nanotubes can be obtained; Secondly, the method based on the adsorption process in inner cavities is of importance for investigation of other physico-chemical pro- cesses inside the cavities of carbon nanotubes. And finally, if combining with other characterization methods, complete structural information

关键词： carbon nanotubes (CNTs) innerdiameter distribution gas adsorption method adsorption isotherm.

Periodic surface structures fabricated by one excimer laser pulse through a silica phase mask grating

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Chinese Science Bulletin 1997年第21期42卷 1787-1792页

作者： Luo, GP Wu, hm Wang, m Zhu, SN Lu, YQ Liu, ZG Wei, Y ming, NB Nanjing Univ Natl Lab Solid State Microstruct Nanjing 210093 Peoples R China

RECENTLY, excimer laser devices, which have higher-energy density output in pulsed ultravio-let (UV) light (193 or 248 nm), have been employed to generate some thin film struc-tures. The principle of this technique, which is also called pulsed laser deposition (PLD)technique, is that the exposed material could be ablated out of the sample by the focusing laserbeam, and redeposit on a substrate to form thin-film structures under certain conditions. Onthe other hand, researchers have obtained high-quality Bragg gratings in photosensitive

关键词： excimer laser pulse phase mask grating periodic surface structures.

Identification of serum proteins discriminating colorectal cancer patients and healthy controls using surface-enhanced laser desorption ionisation-time of flight mass spectrometry

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World Journal of Gastroenterology 2006年第10期12卷 1536-1544页

作者： Judith YmN Engwegen Helgi H Helgason Annemieke Cats Nathan Harris Johannes mG Bonfrer Jan hm Schellens Jos H Beijnen Department of Pharmacy and Pharmacology The Netherlands Cancer Institute/Slotervaart Hospital AmsterdamThe Netherlands Department of medical Oncology The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital AmsterdamThe Netherlands Department of Gastroenterology and Hepatology The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital AmsterdamThe Netherlands Ciphergen Biosystems Inc. Freemont CaliforniaUnited States Department of Clinical Chemistry The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital AmsterdamThe Netherlands Utrecht University Faculty of Pharmaceutical Sciences Department of Biomedical analysisUtrechtThe Netherlands

AIm： To detect the new serum biomarkers for colorectal cancer （CRC） by serum protein profiling with surfaceenhanced laser desorption ionisation - time of flight mass spectrometry （SELDI-TOF mS）. mETHODS： Two independent serum sample sets were analysed separately with the ProteinChip technology （set A： 40 CRC ＋ 49 healthy controls; set B： 37 CRC ＋ 31 healthy controls）, using chips with a weak cation exchange moiety and buffer pH 5. Discriminative power of differentially expressed proteins was assessed with a classification tree algorithm. Sensitivities and specificities of the generated classification trees were obtained by blindly applying data from set A to the generated trees from set B and vice versa. CRC serum protein profiles were also compared with those from breast, ovarian, prostate, and non-small cell lung cancer. RESULTS： mass-to-charge ratios （m/z） 3.1×10^3, 3.3× 10^3, 4.5×10^3, 6.6×10^3 and 28×10^3 were used as classitiers in the best-performing classification trees. Tree sensitivities and specificities were between 65% and 90%.Host of these discriminative m/z values were also different in the other tumour types investigated. m/z 3.3× 10^3, main classifier in most trees, was a doubly charged form of the 6.6× 10^3-Da protein. The latter was identified as apolipoprotein C-I. m/z 3.1×10^3 was identified as an N-terminal fragment of albumin, and m/z 28× 10^3 as apolipoprotein A-I. CONCLUSION： SELDI-TOF mS followed by classification tree pattern analysis is a suitable technique for finding new serum markers for CRC. Biomarkers can be identified and reproducibly detected in independent sample sets with high sensitivities and specificities. Although not specific for CRC, these biomarkers have a potential role in disease and treatment monitoring.

关键词： Proteomics Colorectal cancer Biomarker Sensitivity Specificity

香烟提取物诱导牛冠状动脉内皮细胞损伤的机制

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环境与健康杂志 2008年第9期25卷 776-778,F0003页

作者：战威 SHEETS Sm WONG L mARTINS-GREEN m CASIANO CA FLETCHER hm 天津医科大学公共卫生学院天津300070 美国罗马林达大学医学院微生物学与生物化学系美国加州大学里弗塞德分校细胞生物学与神经生物学系

目的通过观察香烟提取物对牛冠状动脉内皮细胞(BCAEC)的损伤作用,为研究吸烟与心血管疾病之间的关系提供依据。方法以吸烟者体内通常的尼古丁浓度为基准,分别用尼古丁、香烟主流烟雾提取物(mSW)和侧流烟雾提取物(SSW)对 BCAEC 进行染毒... 详细信息

目的通过观察香烟提取物对牛冠状动脉内皮细胞(BCAEC)的损伤作用,为研究吸烟与心血管疾病之间的关系提供依据。方法以吸烟者体内通常的尼古丁浓度为基准,分别用尼古丁、香烟主流烟雾提取物(mSW)和侧流烟雾提取物(SSW)对 BCAEC 进行染毒(尼古丁染毒终浓度分别为1×10^(-5)、0.8×10^(-5)、0.9×10^(-5)mol/L),用显微数码成像系统记录细胞形态变化,检测凋亡细胞与坏死细胞的发生比例,测定 caspase 酶活力。结果经尼古丁、mSW 处理的 BCAEC 形态上发生凋亡样变化,处理24 h 后凋亡细胞发生率为5.89%和11.94%。而 SSW 处理后的 BCAEC 呈坏死样变化.处理24 h 后62.84%的细胞发生坏死。尼古丁和 mSW 可诱导 caspase-3活力增高。结论香烟提取物可诱导牛冠状动脉内皮细胞损伤,mSW 可引起细胞凋亡,SSW 导致细胞坏死,caspase-3的激活可能是香烟提取物诱导牛冠状动脉内皮细胞凋亡的机制。

关键词：烟草烟污染香烟提取物尼古丁牛冠状动脉内皮细胞细胞损伤细胞凋亡细胞坏死

NOD鼠胸腺及外周淋巴细胞亚群变化与年龄关系

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中华微生物学和免疫学杂志 1994年第4期14卷 272-276页

作者：张志利 Geor.,hm 山西医学院第一附属医院

ＮＯＤ鼠可自发地患糖尿病，该鼠的发病与自身免疫性淋巴细胞对胰岛的浸润，从而引起胰岛β细胞的不断破坏有关。这种破坏可能是由Ｔ淋巴细胞所引起的。雌性ＮＯＤ鼠胸腺及外周淋巴细胞随年龄的增加胸腺细胞数量减少而脾细胞数量增加。... 详细信息

ＮＯＤ鼠可自发地患糖尿病，该鼠的发病与自身免疫性淋巴细胞对胰岛的浸润，从而引起胰岛β细胞的不断破坏有关。这种破坏可能是由Ｔ淋巴细胞所引起的。雌性ＮＯＤ鼠胸腺及外周淋巴细胞随年龄的增加胸腺细胞数量减少而脾细胞数量增加。用ＣＤ４／ＣＤ８双抗双标检测显示，雌性ＮＯＤ糖尿病鼠胸腺双阴性与单阳性细胞的比例增加而双阳性细胞的比例减少。外周ＣＤ４比ＣＤ８阳性细胞的比值在胰岛炎期增高，在糖尿病期下降。

关键词： NOD小鼠自身免疫淋巴细胞亚群