Dose-dependent effect of histamine on antibody generation in vivo

作     者：Tripathi T Shahid M Khan HM Khan RA Siddiqui MU 

作者机构：DePartment of BiochemistryFaculty of MedicineJawaharlal Nehru Medical College and HospitalAligarh Muslim University Department of MicrobiologyFaculty of MedicineJawaharlal Nehru Medical College and HospitalAligarh Muslim University Department of PharmacologyFaculty of MedicineJawaharlal Nehru Medical College and HospitalAligarh Muslim University Department of BiochemistryFaculty of MedicineJawaharlal Nehru Medical College and HospitalAligarh Muslim University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2010年第3卷第2期

页      面：112-116页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：University Grants Commission, New Delhi,India for providing UGC Fellowship[UGC letter DON F.19-33/2006(CU)] M.Shahid is grateful to Department of Science & Technology,Ministry of Science & Technology,Government of India for awarding"Young Scientist Project Award"(FT/SR-L-111/2006) 

主　　题：Histamine Immumomodulation T-cell dependent antibody response Humoral immune response SRBC 

摘      要：Objective:To delineate immunomodulatory role of histamine on antibody generation pr of ile in rabbit in the present dose-dependent histamine ***:The cohort comprised of three groups(Ⅲ,ⅣandⅤ),containing six rabbits each,and received subcutaneous histamine 50μg/kg×bis in die(b.i.d.),100μg/kg×*** 200μg/kg X b.i.d.,respectively for 10 days (starting from the 1st day).They were subsequently immunized on the 3rd day with intravenous injection of sheep blood cell(SRBC)(1×10 cells/mL).GroupⅡ(positive control)(n=6) received vehicle(sterile distilled water) and immunized at day 3 similarly while groupⅠ(negative control) (n=6) remained non-immunized and received only *** experimentations were performed in *** samples were collected on pre-immunization(pre-I)(day 0),as well as on days 7-,14-,21-,28- and 58- post-immunization(post-I).Immunological parameters[total immunoglobulins(Igs),IgM and IgG]were analyzed by enzyme linked immunosorbent assay (ELISA) ***:Histamine could influence a detectable antibody response to SRBC as early as day 7-post-I,which lasted until day 58- *** results were found statistically significant(P 0.05).Conclusions:Our results provide evidence that histamine has a short-term effect on antibody generation(until its presence in the body),and the antibody generation titer in vivo were affected by the concentration of histamine.