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Plasma synthesis of various polymorphs of tungsten trioxide nanoparticles using gliding electric discharge in humid air:characterization and photocatalytic properties

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Plasma Science and Technology 2023年第12期25卷 72-82页

作者： Romaric L SEUTCHA Georges KAmGANG-YOUBI Elie ACAYANKA valeria VERmILE François DEVRED Eric m GAIGNEAUX Samuel LAmINSI Inorganic Chemistry Department Faculty of SciencesUniversity of Yaounde INgoa-Ekelle 812YaoundéCameroon Institute of Condensed matter and Nanosciences(ImCN) Molecular ChemistryMaterials and CatalysisUniversitéCatholique de LouvainPlace Louis Pasteur 11348 Louvain-la-NeuveBelgium

A gliding electric arc(glidarc)discharge generates a low-temperature plasma at atmospheric *** the discharge occurs in humid air as the feed gas,the chemistry of a glidarc plasma consists of in situ formation of HO°and NO°as the primary chemical *** trioxide(WO_(3))nanoparticles were successfully prepared by exposure of a liquid precursor to glidarc *** WO_(3)samples were calcined at three different temperatures(300℃,500℃and 800℃),resulting in different pure polymorphs:γ-WO_(3)(at 300℃),β-WO_(3)(at 500℃)andα-WO_(3)(at 800℃)according to x-ray diffraction *** identification of WO_(3)compounds was also confirmed by attenuated total reflection Fourier transform infrared spectroscopy *** in the calcination temperature of WO_(3)induced a decrease in its specific surface area according to Brunauer–Emmett–Teller nitrogen physisorption *** UV-visible results showed that the absorption bands of plasma-WO_(3)samples were more intense than those of WO_(3)samples obtained by a precipitation route,a classical method used for ***,this parameter can improve the photocatalytic properties of WO_(3)under visible *** photodegradation(in sunlight conditions)of gentian violet,chosen as a model pollutant,confirmed the photocatalytic properties of plasma-WO_(3)*** novel synthesis method has great potential to improve the efficiency of advanced tungsten trioxide-based functional material preparation,as well as in pollution-reducing and energy-saving tungsten extractive metallurgy.

关键词： plasma glidarc tungsten trioxide plasma-synthesis nanoparticles photocatalyst

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morphometric and kinematic sperm subpopulations in split ejaculates of normozoospermic men

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Asian Journal of Andrology 2016年第6期18卷 831-834页

作者： Pilar Santolaria Caries soler Pilar Recreo Teresa Carretero Araceli Bono Jose m Berne Jesus L Yaniz TECNOGAm research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences University of Zaragoza Huesca Spain Department of Functionat 8iotogy and Physical Anthropology University of Valencia 46100 Burjassot Valencia Spain R+D Department Proiser R+D Scientific Park University of Valencia 46980 Paterna Valencia Spain TECNOGAm research group Environmental Sciences Institute (IUCA) Departament of Human Anatomy and Histolosy Faculty of Health and Sport Sciences of Huesca PLaza Universidad 3 22002 Huesca Spain TECNOGAm research group Environmental Sciences Institute (IUCA) Hospital General San Jorge de Huesca Area de Urologia Av. Martinez de Velasco 36 22004 Huesca Spain

This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis （CASA-mot）, and of sperm morphometry by computer-assisted sperm morphometry analysis （CASA-morph） using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kinematic subpopulations （slow with low ALH [35.6% of all motile spermatozoa], with circular trajectories [32.0%], and rapid with high ALH [32.4%]）, and three morphometric subpopulations （large-round [33.9% of all spermatozoa], elongated [32.0%], and small [34.10%]）. The distribution of kinematic sperm subpopulations was different among ejaculate fractions （P 〈 0.001）, with higher percentages of spermatozoa exhibiting slow movements with low ALH in the second and third portions, and with a more homogeneous distribution of kinematic sperm subpopulations in the first portion. The distribution of morphometric sperm subpopulations was also different among ejaculate fractions （P〈 0.001）, with more elongated spermatozoa in the first, and of small spermatozoa in the third, portion. It is concluded that important variations in the distribution of kinematic and morphometric sperm subpopulations exist between ejaculate fractions, with possible functional implications.

关键词： man sperm morphometry by computer-assisted sperm morphometry analysis sperm motility by computer-assisted spermanalysis sperm subpopulations split ejaculate

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Application of two forms of silicon and their impact on the postharvest and the content of bioactive compounds in cucumber(Cucumis sativus L.)fruits

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BIOCELL 2022年第11期46卷 2497-2506页

作者： YOLANDA GONZÁLEZ-GARCÍA valeria FLORES-ROBLES GREGORIO CADENAS-PLIEGO ADALBERTO BENAVIDES-mENDOZA mARCELINO CABRERA DE LA FUENTE ALBERTO SANDOVAL-RANGEL ANTONIO JUÁREZ-mALDONADO Departamento de Botánica Universidad Autónoma Agraria Antonio NarroSaltillo25315México maestría en Ciencias en Horticultura Universidad Autónoma Agraria Antonio NarroSaltillo25315México Centro de Investigación en Química Aplicada Saltillo25294México Departamento de Horticultura Universidad Autónoma Agraria Antonio NarroSaltillo25315México

The metabolic activity of the fruits continues even after harvest,which results in the loss of bioactive compounds,a decrease in the quality of the fruits,softening and browning,among other negative *** use of certain elements such as silicon can improve postharvest quality,since it is involved in the metabolic,physiological and structural activity of plants,moreover can increase the quality of the *** addition,nanotechnology has had a positive impact on crop yield,nutritional value,fruit quality and can improve antioxidant *** these reasons,the use of beneficial elements such as silicon in the form of nanoparticles can be a viable option to improve the characteristics of the *** the present study was evaluated the application of potassium silicate(125,250 and 500 mg L^(−1))and SiO_(2) nanoparticles(125,250 and 500 mg L^(−1))during the development of the *** results showed that the application of silicon(potassium silicate and silicon nanoparticles)increased the content of total soluble solids(up to 15.6%higher than control),titratable acidity(up to 38.8%higher than control),vitamin C(up to 78.2%higher than control),phenols(up to 22%higher than control),flavonoids(up to 64.6%higher than control),and antioxidant activity in lipophilic compounds(up to 56.2%higher than control).This study suggests that the use of silicon can be a good option to increase the content of bioactive compounds in cucumber fruits when they are applied during the development of the crop.

关键词： Fruit quality Nanotechnology Silicon nanoparticles Potassium silicate Antioxidant compounds Antioxidant capacity

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Current status and potential of morphometric sperm analysis

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Asian Journal of Andrology 2016年第6期18卷 863-870页

作者： Alejandro maroto-morales Olga Garcia-Alvarez manuel Ramon Felipe martinez-Pastor m Rocio Fernandez-Santos A Josefa soler Jose Julian Garde SaBio IREC (CSIC - UCLm -JCCm) Albacete Spain Biomedical Center Medical Faculty in Pilsen Charles University in Prague Pilsen Czech Republic Regional Center of Animal Selection and Reproduction (CERSYRA) JCCm Valdepenas Spain 4Institute for Animal Health and Cattle Development University of Leon LeOn Spain Department of molecular Biology University of Leon Leon Spain Faculty of Pharmacy (UCLm) Albacete Spain

The spermatozoon is the most diverse cell type known and this diversity is considered to reflect differences in sperm function. How the diversity in sperm morphology arose during speciation and what role the different specializations play in sperm function, however, remain incompletely characterized. This work reviews the hypotheses proposed to explain sperm morphological evolution, with a focus on some aspects of sperm morphometric evaluation; the ability of morphometrics to predict sperm cryoresistance and male fertility is also discussed. For this, the evaluation of patterns of change of sperm head morphometry throughout a process, instead of the study of the morphometric characteristics of the sperm head at different stages, allows a better identification of the males with different sperm cryoconservation ability. These new approaches, together with more studies employing a greater number of individuals, are needed to obtain novel results concerning the role of sperm morphometry on sperm function. Future studies should aim at understanding the causes of sperm design diversity and the mechanisms that generate them, giving increased attention to other sperm structures besides the sperm head. The implementation of scientific and technological advances could benefit the simultaneous examination of sperm phenotype and sperm function, demonstrating that sperm morphometry could be a useful tool for sperm assessment.

关键词： computer-assisted sperm morphometric analysis mammals sperm function sperm morphometry

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Pupal emergence pattern in cactophilic Drosophila and the effect of host plants

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Insect Science 2018年第6期25卷 1108-1118页

作者： Eduardo m.Soto Julián Padrò Pablo milla Carmona Diego T.Tuero valeria P.Carreira Ignacio m.Soto Departamento de Ecologia Genética y Evoluciòn Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresIEGEBA-CONICETBuenos AiresArgentina

Drosophila buzzatii and *** are sibling cactophilie *** former breeds primarily on prickly pears (genus Opuntia)whereas the latter breeds on columnar cacti of the genera Cereus and Trichocereus,although with certain degree of niche *** examined the interspecific differences in diurnal temporal patterns of adult emergence from puparia and evaluated whether this behavior is affected by rearing in the different cactus hosts available in *** detected important host-dependent genetic variation for this trait differentially affecting the emergence schedule of these *** pattern of emergence time was directly correlated with developmental time and negatively correlated with adult wing size,suggesting that early emergences are at least indirectly correlated with increased *** discussed our results in terms of their putative effects on fitness and the genetic-metabolic pathways that would be presumably affected by host's nutritional-chemical differences.

关键词： alkaloids cactus circadian cycle life history traits phenotypic plasticity pupae

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Application of None Cement Aluminamagnesia Gunning mix with Special High Reactive Alumina in Steel Ladles

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China's Refractories 2020年第1期29卷 20-26页

作者： Jérome KIENNEmANN Paul soler Johnson LEE Alteo Gardanne 20062France

During calcining the aluminum hydroxide according to the Bayer process in a rotary kiln,the process of incomplete conversion to alpha alumina by soft calcination leads to a special intermediate alumina product containing microcrystalline of primary crystal alpha alumina particles less than 0.4μm,and other alumina phases with finer microcrystalline of less than 0.2μm *** special alumina powder has similar properties to silica fume or clay,such as high surface area and reactivity,fast wetting,low water demand and high *** can partially or completely replace silica fume and clay in dry gunning *** works towards improving the high temperature resistance of the gunning material matrix as well as the corrosion resistance and service life of the gunning *** application results show that:the ladle cementfree alumina-magnesia dry gunning mix with this special alumina sub-micro powder,compared with traditional dry gunning materials,has lower rebound loss(milar performance.A further improvement goal is to replace the initial casting linings,thus this concept will give a new revolution to the monolithic steel ladles.

关键词： none cement gunning mix special reactive alumina monolithic steel ladles

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Comparison of different statistical approaches to evaluate morphometric sperm subpopulations in men

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Asian Journal of Andrology 2016年第6期18卷 819-823页

作者： Jesus L Yaniz Sandra Vicente-Fiel Caries soler Pilar Recreo Teresa Carretero Araceli Bono Jose m Berne Pilar Santolaria TECNOGAm research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences Higher Polytechnic School University of Zaragoza Ctra. Cuarte S/N 22071 Huesca Spain Department of Functional Biology and Physical Anthropology University of Valencia 46100 Burjassot Valencia Spain R+D Department Proiser R+D Scientific Park University of Valencia 46980 Paterna Valencia Spain TECNOGAm research group Environmental Sciences Institute (IUCA) Departament of Human Anatomy and Histology Faculty of Health and Sport Sciences of Huesca Plaza Universidad 3 22002 Huesca Spain TECNOGAm research group Environmental Sciences Institute (IUCA) Hospital General San Jorge de Huesca Area de Urologla Av. Martfnez de Velasco 36 22004 Huesca Spain

This study was designed to characterize morphometric sperm subpopulations in normozoospermic men by using different statistical methods and examining their suitability to classify correctly different sperm nuclear morphologies present in human ejaculates. Ejaculates from 21 normozoospermic men were collected for the study. After semen collection and analysis, samples were prepared for morphometric determination. At least 200 spermatozoa per sample were assessed for sperm morphometry by computer-assisted sperm morphometry analysis （CASA-morph） using fluorescence. Clustering and discriminant procedures were performed to identify sperm subpopulations from the morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three morphometric subpopulations （large-round 30.4%, small-round 46.6%, and large-elongated 22.9%）. In the second analysis, using discriminant methods, the classification was made independently of size and shape. Three morphological categories according to nuclear size （small 〈10.90 μm^2, intermediate 10.91-13.07 μm^2, and large 〉13.07 μm^2） and four categories were defined on 400 canonical cells （100 × 4） from 10 men according to sperm nuclear shape （oval, pyriform, round, and elongated）. Thereafter, the resulting classification functions were used to categorize 4200 spermatozoa from 21 men. Differences in the class distribution were observed among men from both clustering and discriminant procedures. It was concluded that the combination of CASA-morph fluorescence-based technology with multivariate cluster or discriminant analyses provides new information on the description of different morphometric sperm subpopulations in normal individuals, and that important variations in the distribution of morphometric sperm subpopulations may exist between men, with possible functional implications.

关键词： computer-assisted sperm morphometry analysis man sperm subpopulations

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Subcellular Redistribution of Root Aquaporins nduced by Hydrogen Peroxide

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molecular Plant 2015年第7期8卷 1103-1114页

作者： michael m. Wudick Xiaojuan Li valeria Valentini Niko Geldner Joanne Chory Jinxing Lin Christophe maurel Doan-Trung Luu Biochimie et Physiologie moleculaire des Plantes Institut de Biologie Integrative des Plantes UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Universit~Montpellier 2 2 Place Viala F-34060 Montpellier Cedex 2 France Key Laboratory of Plant molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China Department of Plant molecular Biology Universite de Lausanne 1015 Lausanne Switzerland Plant molecular and Cellular Biology Laboratory The Salk Institute La Jolla CA 92037 USA Present address: Department of Cell Biology and molecular Genetics University of Maryland College Park MD 20742-5815 USA Present address: Instituto Gulbenkian de Ciencia 2780-156 Oeiras Portugal

Aquaporins are water channel proteins that mediate the fine-tuning of cell membrane water permeability during development or in response to environmental stresses. The present work focuses on the oxidative stress-induced redistribution of plasma membrane intrinsic protein （PIP） aquaporins from the plasma membrane （Pm） to intracellular membranes. This process was investigated in the Arabidopsis root. Su- crose density gradient centrifugation showed that exposure of roots to 0.5 mm H2O2 induces significant depletion in Pm fractions of several abundant PIP homologs after 15 rain. Analyses by single-particle tracking and fluorescence correlative spectroscopy showed that, in the Pm of epidermal cells, H2O2 treat- ment induces an increase in lateral motion and a reduction in the density of a fluorescently tagged form of the prototypal AtPIP2;1 isoform, respectively. Co-expression analyses of AtPIP2;1 with endomembrane markers revealed that H2O2 triggers AtPIP2;1 accumulation in the late endosomal compartments. Life- time analyses established that the high stability of PIPs was maintained under oxidative stress conditions, suggesting that H2O2 triggers a mechanism for intracellular sequestration of Pm aquaporins without further degradation. In addition to information on cellular regulation of aquaporins, this study provides novel and complementary insights into the dynamic remodeling of plant internal membranes during oxida- tive stress responses.

关键词： Arabidopsis aquaporin stress reactive oxygen species root

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Regionalization of epididymal duct and epithelium in rats and mice by automatic computer-aided morphometric analysis

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Asian Journal of Andrology 2005年第3期7卷 267-275,页

作者： C.soler J.J.demonserrat m.Núez R.Gutiérrez J.Núez m.Sancho T.G.Cooper Departmentof Functional Biology and Physical Anthropology University of ValènciaValència 46112Spain Departamentode Ouímica y Biología Universidad del NorteBarranquillaColombia Instituteof Reproductive medicine University of MünsterMünster D-48129Germany

Aim： To establish a rat and mouse epididymal map based on the use of the Epiquatre automatic software for histologic image analysis. methods： Epididymides from five adult rats and five adult mice were fixed in alcoholic Bouin＇s fixative and embedded in paraffin. Serial longitudinal sections through the medial aspect of the organ were cut at 10 jam and stained with hematoxylin and eosin. As determined from major connective tissue septa, nine subdivisions of the rat epididymis and seven for the mouse were determined, consisting of five sub-regions in the caput （rat and mouse）, one （mouse） or three （rat） in the corpus and one in the cauda （rat and mouse）. Using the Epiquatre software, several tubular, luminal and epithelial morphometric parameters were evaluated. Results： Statistical comparison of the quantitative parameters revealed regional differences （2-5 in the rat, 3-6 in the mouse, dependent on parameters） with caput regions 1 and 2 being largely distinguishable from the similar remaining caput and corpus, which were in turn recognizable from the cauda regions in both species. Conclusion： The use of the Epiquatre software allowed us to establish regression curves for different morphometric parameters that can permit the detection of changes in their values under different pathological or experimental conditions. （Asian J Androl 2005 Sep; 7： 267-275）

关键词： rat mouse epididymis morphometry zonation computer-aided image analysis Epiquatre Software

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Phylogeny,genetics,and the partial life cycle of Oncomegas wageneri in the Gulf of mexico

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Current Zoology 2020年第3期66卷 275-283页

作者： Andrés mARTINEZ-AQUINO Víctor m.VIDAL-mARTINEZ F.Sara CECCARELLI Oscar mENDEZ Lilia C.soler-JImENEZ m.Leopoldina AGUIRRE-mACEDO Facultad de Ciencias Universidad Autonoma de Baja CaliforniaCarretera Transpeninsular 3917Fraccionamiento PlayitasEnsenadaBaja California 22860Mexico Laboratorio de Patologia Acuatica Departamento de Recursos del MarCentro de Investigacion y de Estudios Avanzados del Instituto Politecnico NacionalUnidad MeridaCordemexCarretera Antigua a Progreso Km.6MeridaYucatan 97310Mexico Departamento de Biologia de la Conservacion CONACYT-Centro de Investigacion Cientifica y de Educacion Superior de EnsenadaCarretera Ensenada-TjuanaEnsenadaBaja California 22860Mexico Laboratorio de Hidrobiologia Facultad de Biologia-XalapaUniversidad VeracruzanaCircuito Gonzalo Aguirre Beltran S/N Zona UniversitariaXalapaVeracruz 91090Mexico

Despite the diversity and ecological importance of cestodes,there is a paucity of studies on their life stages(i.e.,complete lists of intermediate,paratenic,and definitive hosts)and genetic *** example,in the Gulf of mexico(Gom)98 species of cestodes have been reported to date;however,data on their intraspecific genetic variation and population genetic studies are *** trypanorhynch cestode,Oncomegas wageneri,is found(among other places)off the American Western Atlantic Coast,including the Gom,and has been reported as an adult from stingrays and from several teleost species in its larval form(as plerocerci).This study represents the first report of 2 previously unregistered definitive hosts for ***,namely the Atlantic sharpnose shark Rhizoprionodon terraenovae and the southern stingray Hypanus *** this work,partial sequences of the 28S(region D1-D2)ribosomal DNA were analyzed to include *** within an eutetrarhynchoid phylogenetic *** *** individuals(which included plerocerci and adults)were recovered as monophyletic and Oncomegas celatus was identified as the sister species of ***,population genetic analyses of *** from the southern Gom were carried out using DNA sequences of the mitochondrial cytochrome c oxi-dase subunit 1(COI)gene,which reflected high genetic variation and a lack of genetic structure among the 9 oceanographic sampling *** on these results,*** is panmictic in the southern *** extensive sampling along the species entire distribution is necessary to make more accurate inferences of population genetics of ***.

关键词： barcode Cyclopsetta chittendeni population genetics Hypanus americanus Oncomegas Rhizoprionodon terraeno-vae 28S rDNA(D1-D2)

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