Regionalization of epididymal duct and epithelium in rats and mice by automatic computer-aided morphometric analysis

作     者：C.Soler J.J.deMonserrat M.Núez R.Gutiérrez J.Núez M.Sancho T.G.Cooper 

作者机构：Departmentof Functional Biology and Physical AnthropologyUniversity of ValènciaValència 46112Spain Departamentode Ouímica y BiologíaUniversidad del NorteBarranquillaColombia Instituteof Reproductive MedicineUniversity of MünsterMünster D-48129Germany 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2005年第7卷第3期

页      面：267-275,页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：This work was partially funded by Dirección General de Investigación Ciencia y Técnica the Conselleria de Cultura Educació i Ciència de la Generalitat Valenciana(PS88-0116 and 683.3-130,respectively) 

主　　题：rat mouse epididymis morphometry zonation computer-aided image analysis Epiquatre Software 

摘      要：Aim： To establish a rat and mouse epididymal map based on the use of the Epiquatre automatic software for histologic image analysis. Methods： Epididymides from five adult rats and five adult mice were fixed in alcoholic Bouin＇s fixative and embedded in paraffin. Serial longitudinal sections through the medial aspect of the organ were cut at 10 jam and stained with hematoxylin and eosin. As determined from major connective tissue septa, nine subdivisions of the rat epididymis and seven for the mouse were determined, consisting of five sub-regions in the caput （rat and mouse）, one （mouse） or three （rat） in the corpus and one in the cauda （rat and mouse）. Using the Epiquatre software, several tubular, luminal and epithelial morphometric parameters were evaluated. Results： Statistical comparison of the quantitative parameters revealed regional differences （2-5 in the rat, 3-6 in the mouse, dependent on parameters） with caput regions 1 and 2 being largely distinguishable from the similar remaining caput and corpus, which were in turn recognizable from the cauda regions in both species. Conclusion： The use of the Epiquatre software allowed us to establish regression curves for different morphometric parameters that can permit the detection of changes in their values under different pathological or experimental conditions. （Asian J Androl 2005 Sep; 7： 267-275）