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Journal of Fisheries Science 2020年第1期2卷 17-21页

作者： Natalino da Costa Sousa José Araújo da silva Emilly monteiro Lopes Arthur Felipe Lima dos Santos Francisco Alex Lima Barros Carlos Alberto martins Cordeiro Peterson Emmanuel Guimarães Paixão Estela dos Santos medeiros João Carlos Nunes de Souza márcia valéria silva do couto Federal University of Pará(UFPA) Campus BragançaAlameda Leandro RibeiroBragança68600-000PABrazil Tiradentes University(UNIT)-Av.murilo Dantas 300-FarolândiaCEP 49032-490AracajuSergipeBrazil Faculdade Pio Décimo Av.Pres.Tancredo Neves 5655-Jabotiana49095-000Aracaju-SE.Brazil

This study evaluated the effect of enriched artemia nauplii with commercial probiotic for angelfish larvae determining growth perfomance,survival and modulation of the intestinal ***,it experiment occurred in completely randomized design with five treatments(T1-0.0,T2-1.5,T3-3.0,T4-4.5 and T5-6.0g of commercial probiotic)and four replaces during 20 *** larvaculture,post larvae passed by biometric procedures to determine productive performance and then microbiological *** reduction of total heterotrophic bacteria while increased lactic acid bacteria in the intestinal tract from the post larvae for treatments T3,T4 and *** commercial probiotic also increased the survival and performance as final weight,weight gain and specific growth *** these reasons,the use of 3g of commercial probiotic promotes greater performance and intestinal modulation for angelfish larvae.

关键词： Ornamental fish Probiotic Larvaculture

Chemical Exposure Hazardous for Fish Hyphessobrycon eques through the Incorrect Release of Oil in Amazon Region

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Journal of Fisheries Science 2020年第2期2卷 19-23页

作者： Natalino da Costa Sousa Arthur dos Santos da silva Keber Santos Costa Junior Francisco Alex Lima Barros Geisy Correa de Oliveira Pedro Rodrigo Nery de Souza Claudmilson Rodrigues de mesquita Bruna de jesus Santos Alexander Damaceno Lima santos Winnícius muniz dos Santos Sá Nayara Siqueira dos Santos márcia valéria silva do couto Federal University of Pará(UFPA) Campus BragançaAlameda Leandro RibeiroBragança68600-000PABrazil Federal University of Pará(UFPA) Campus de TucuruíRua Itaipu36-Vila PermanenteTucuruí68464-000PABrazil Federal University of de Sergipe(UFS) Av.Marechal Rondons/n-Jardim Rosa ElzeSão Cristóvão49100-000SEBrasil Faculdade Pio Décimo Av.Pres.Tancredo Neves 5655-Jabotiana49095-000Aracaju–SE.Brazil

Incorrect discard of oil used by fishing ship in the brazilian north region has become a common *** the possible hazard to the aquatic organisms,still missing scientific data about their ***,this study aimed to evaluate the stress and lethality caused by lubricant oil(FSAOLU)on fish Hyphessobrycon ***,it was used six different concentration diluted in water(0,22,24,26,28,30 and 32%of oil)and three replaces during 96 *** the end or during the experiment(with dying fish),it was collected blood samples(cutting the caudal fin)to determine physiological *** FSAOLU showed mean lethal dose(LD50-96h)of 27.36%,classified as toxic causing alterations in glucose values from the 26%.The greater dilution of FSAOLU(32%)increased 115%in glucose values when compared to the ***,lubricant oil when discarded in water,it present hazard to the aquatic organisms causing stress and mortality for fish being necessary adequate management to discard of this residue.

关键词： Toxicity FSA Sensibility Stress

modeling of Fluidized Bed Gasification:Assessment of Zero-dimensional and CFD Approaches

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Journal of Thermal Science 2015年第4期24卷 378-385页

作者： Nuno couto valter silva Eliseu monteiro P.S.D.Brito Abel Rouboa University of Tras-os-montes and Alto douro INEGI/Faculty of Engineering University of Porto C3i-Interdisciplinary Center for Research and Innovation Polytechnic Institute of PortalegreLugar da AbadessaApartado 1487301-901PortalegrePortugal mEAm Department University of Pennsylvania

In modeling fluidized bed gasification experiments,equilibrium and CFD models are valuable *** existence of multi-dimensional effects inside the reactor vessel due to the kinetics of the process and the fluid dynamics phenomena could result in deviation from the zero-dimensional *** models integrating kinetics and hydrodynamics are being developed by using a computer fluid dynamics(CFD)*** objective of this investigation is to assess and compare the adequacy of zero-dimensional and CFD approaches in modeling fluidized bed gasification regarding a semi-industrial scale(numerical results are validated under experimental runs).Results show that the zero-dimensional model based on the approach of dual stage equilibrium performs reasonably well in adequately predicting the product gas composition at different operating conditions and for different feedstocks,although with quantitative ***,the discrepancy depends on the oxygen content of the oxidation agent and on the steam-to biomass ratio decreasing when these parameters *** models provide deeper information being able to estimate the syngas composition or other operating parameter at any point of space and *** of some quantitative discrepancy,the zero-dimensional modeling approach is deemed satisfactory from the viewpoint of the determining design conditions simulation.

关键词： Gasification CFD model Dual stage equilibrium model

Influence of physical-anatomical wood variables on charcoal physical-mechanical properties

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Journal of Forestry Research 2023年第2期34卷 531-538页

作者： Allan motta couto Thiago Campos monteiro Paulo Fernando Trugilho JoséTarcísio Lima JoséReinaldo moreira da silva Alfredo Napoli Diego Pierre de Almeida Department of Forestry Science State University of Mato Grosso do Sul–UEMSAv.Graziela Maciel BarrosoKm 12 Zona RuralAquidauanaMS 79200000Brazil Department of Forestry Engineering and Technology Federal University of Paraná–UFPRA V.Pref.Lothário Meissner 632Jardim BotânicoCuritibaPR 80210170Brazil Department of Forestry Science Federal UniversityLavras–UFLALavrasMG 37200000Brazil Département de Performance Des Systèmes de Production Et de Transformation Tropicaux Centre de Coopération Internationale em Recherche Agronomique pour le Développement–CIRADMontpellierFrance

This research assessed the relationship between wood physical-anatomical variables and charcoal physical-mechanical *** trees were harvested and mechanically processed into standard pieces for laboratory *** following variables were determined:wood density,fiber length and width,fiber lumen diameter and wall thickness,fiber wall fraction,and vessel *** charcoal was produced in a laboratory and gravimetric yield,relative density,and mechanical properties ***’s correlation coefficients,multivariate statistical analysis of canonical correlation,and principal component analysis were used to evaluate the relationship between wood and charcoal *** width was not significantly correlated with charcoal *** bulk density,hardness,and strength were positively correlated with wood density,fiber length,wall thickness,and wall *** properties were negatively correlated with fiber lumen diameter and vessel *** results will help identify the best wood with adequate physical-mechanical properties for industrial charcoal proces sing.

关键词： Biomass Density Energy Fiber Vessel

Cloning and characterization of gene-resistant analogs(RGAs)involved in rust(Puccinia psidii) resistance in Eucalyptus grandis

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Journal of Forestry Research 2015年第3期26卷 711-718页

作者： marcelo Luiz Laia Acelino couto Alfenas Sergio Hermínio Brommonschenkel Shinitiro Oda Eduardo José de melo Inaê mariêde Araú jo silva Janaína Fernandes Gonalves Ariadne marques Departamento de Engenharia Florestal Universidade Federal dos Vales do Jequitinhonha e Mucuri DiamantinaMinas Gerais 39100-000 Brazil Departamento de Fitopatologia Universidade Federal de Viosa Viosa Minas Gerais 36570-900 Brazil Suzano Bahia Sul Celulose S/A Suzano So Paulo Brazil

Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. most of the disease-resistant genes encode pro- teins with conserved leucine rich repeat and nucleotide binding site domains. In this study, we cloned and char- acterized gene-resistant analogs （RGAs） from Eucalyptus grandis using degenerate PCR, with primers specifically targeting these two domains. The amplified fragments were cloned into the pGEm-T vector and transformed into Escherichia coli. Among the 90 clones obtained, 13 were sequenced and compared with each other and with previ- ously identified gene-resistant diseases. A BLASTX search in GenBank revealed high similarities among the con- served domains of these cloned genes with RGA genes. Some clones, however, showed no significant similarity with DNA sequences in GenBank. Southern blotting ana- lysis identified several polymorphic RFLP loci between distinct genotypes. However, none of them co-segregated with the Puccinia psidii Winter resistance gene 1 （Ppr1） in a population study.

关键词： Cloning Conserved domains RGAs Rust Eucalyptus

Cerebral ischemia and neuroregeneration

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Neural Regeneration Research 2018年第3期13卷 373-385页

作者： Reggie H.C.Lee michelle H.H.Lee Celeste Y.C.Wu Alexandre couto e silva Harlee E.Possoit Tsung-Han Hsieh Alireza minagar Hung Wen Lin Department of Neurology Louisiana State University Health Science Center Center for Brain Health Louisiana State University Health Science Center Institute of Cellular and System medicine National Health Research Institutes Department of Cellular Biology and Anatomy Louisiana State University Health Science Center Cardiovascular and metabolomics Research Center Hualien Tzu Chi Hospital

Cerebral ischemia is one of the leading causes of morbidity and mortality worldwide. Although stroke （a form of cerebral ischemia）-related costs are expected to reach 240.67 billion dollars by 2030, options for treatment against cerebral ischemia/stroke are limited. All therapies except anti-thrombolytics （i.e., tissue plasminogen activator） and hypothermia have failed to reduce neuronal injury, neurological deficits, and mortality rates following cerebral ischemia, which suggests that development of novel therapies again st stroke/cerebral ischemia are urgently needed. Here, we discuss the possible mechanism（s） underlying cerebral ischemia-induced brain injury, as well as current and future novel therapies （i.e., growth factors, nicotinamide adenine dinucleotide, melatonin, resveratrol, protein kinase C isozymes, pifithrin, hypothermia, fatty acids, sympathoplegic drugs, and stem cells） as it relates to cerebral ischemia.

关键词： cerebral ischemia melatonin resveratrol protein kinase C pifithrin-α fatty acids sympathetic nervous system neuromodulation therapy traditional Chinese therapies stem cel

The“microwave oven”practice in Brazil

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Forensic Sciences Research 2022年第4期7卷 628-632页

作者： Yara Vieira Lemos Luciene menrique Corradi melina Calmon silva Lorena de Oliveira couto Giovanna Hooper Bittencourt Alexander Santos Dionísio Adriana Zatti Lima márcio Alberto Cardoso Eugénia Cunha Forensic Anthropology Laboratory Medico-Legal Institute AndréRoquette in Belo HorizonteMinas GeraisBrazil medical Sciences Faculty of minas Gerais Minas GeraisBrazil Brazilian Association of Forensic Anthropology(ABRAF) BrasíliaBrazil.

This article presents a forensic case of an unusual illegal cremation of a person using a process colloquially known as the“microwave oven”practice in *** microwave process involves two actions:placing the victim in a tire stack and then setting the structure on fire using flammable substances to accelerate the *** practice,identified as“necklacing”,has also been reported in other countries such as South *** report presents a case of microwave oven cremation of a body found in a rural area of minas Gerais,*** forensic work helped determine the biological profile and identity of the victim using radiological *** the microwave oven cremation practice is rare,it can impose challenges for ***,fully understanding this practice can be helpful to the academic and forensic communities.

关键词： Forensic sciences carbonization cremation homicide human identification

Roadmap Proposal for Implementing Building Information modelling (BIm) in Portugal

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Open Journal of Civil Engineering 2016年第3期6卷 475-481页

作者： maria João Falcão silva Filipa Salvado Paula couto Álvaro vale e Azevedo Buildings Department LNEC Lisboa Portugal

Building Information modeling (BIm) is a process involving generation and management of digital representations of physical and functional characteristics of places, which can be exchanged or networked to support decision-making in architecture, engineering and construction (AEC) sector. BIm is a relatively new technology in an industry typically slow to adopt changes, especially in Portugal. The present paper aims to frame the international implementation of BIm in order to propose a roadmap to spread the use of BIm methodologies in Portugal in the next decade.

关键词： BIm Roadmap International Implementation Strategies and Actions

Standardization of BIm Objects: Development of a Proposal for Portugal

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Open Journal of Civil Engineering 2016年第3期6卷 469-474页

作者： Filipa Salvado maria João Falcão silva Paula couto Álvaro vale e Azevedo Buildings Department LNEC Lisbon Portugal

The management of information systems contributes to the economic development of the construction sector by organizing and structuring technical and economic information. It is proposed how BIm (Building Information modelling) and a Portuguese information system-ProNIC (abbreviation for Protocol for the Standardization of Construction Technical Information) may be interconnected. The scope of this information transmission in construction sector is a step to solve several problems that have been identified in conception of BIm models.

关键词： BIm Objects Standardization Technical Information ProNIC

N-acetylcysteine modulates angiogenesis and vasodilation in stomach such as DNA damage in blood of portal hypertensive rats

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World Journal of Gastroenterology 2015年第43期21卷 12351-12360页

作者： Francielli Licks Renata minuzzo Hartmann Camila marques Elizangela Schemitt Josieli Raskopf Colares mariana do couto Soares Juliana Reys Camila Fisher Juliana da silva Norma Possa marroni Universidade Federal do Rio Grande do Sul Instituto de Tecnologia do Paraná Universidade Luterana do Brasil

AIm: To evaluate the antioxidant effect of N-acetylcysteine(NAC) on the stomach of rats with portal ***: Twenty-four male Wistar rats weighing ± 250 g were divided into four experimental groups(n =6 each): Sham-operated(SO),SO + NAC,partial portal vein ligation(PPVL),and PPVL + NAC. Treatment with NAC in a dose of 10 mg/kg(i.p.) diluted in 0.6 m L of saline solution was administered daily for 7 d starting 8 d after the surgery. Animals from the PPVL and SO group received saline solution(0.6 m L) for the same period of time as the PPVL + NAC and SO + NAC group. On the 15 th day the animals were anesthetized and we evaluated portal pressure by cannulating mesenteric artery. After,we removed the stomach for further analysis. We performed immunohistochemical analysis for endothelial nitric oxide synthase(e NOS),vascular endothelial growth factor(VEGF),and nitrotirosine(NTT) proteins in stomach. We also evaluated e NOS and VEGF by Western blot analysis and assessed DNA damage in blood samples by the comet ***: The portal hypertension group exhibited increases in portal pressure when compared to SO group(29.8 ± 1.8 vs 12.0 ± 0.3 mm Hg)(P me was observed when we compared the e NOS(56.8 ± 3.7 vs 13.46 ± 2.8 pixels)(P mmunohistochemistry of the PPVL animals with the SO group. The expression of e NOS(0.39 ± 0.03 vs 0.25 ± 0.03 a.μ)(P valuated by Western blot analysis,and we observed an increase of both proteins on PPVL animals. We also evaluated the DNA damage by comet assay,and observed an increase on damage index and damage frequency on those animals. NAC decreased portal pressure values in PPVL + NAC animals(16.46 ± 2 vs 29.8 ± 1.8 mm Hg)(P mpared to PPVL. The expression of e NOS(14.60 ± 4.1 vs 56.8 ± 3.7 pixels)(P < 0.001

关键词： N-Acetylcysteine Portal hypertension Gastropathy Oxidative stress Antioxidant