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Molecular Plant 2015年第3期8卷 486-488页

作者： Noe Femandez-Pozo Heman G. Rosli Gregory B. Martin lukas A. mueller Boyce Thompson Institute for Plant Research Ithaca NY 14853 USA Instituto de Investigaciones Biotecnolbgicas - Instituto Tecnológico de Chascomús (IIB-INTECH) UNSAM-CONICET Chascomús B71301WA Buenos Aires Argentina Section of Plant Pathology and Plant-Microbe Biology School of Integrative Plant Science Cornell University Ithaca NY 14853 USA

Dear Editor,Virus-induced gene silencing （VIGS） is a fast and powerful method to study gene function in plants （Burch-Smith et al., 2004）. It is based on plant defense mechanisms against viral gene replication and allows high-throughput silencing of genes of inter- est （SenthiI-Kumar and Mysore, 2014）. The molecular mechanisms involved in post-transcriptional gene silencing （PTGS） have been studied intensively, and its steps are well known. The silencing pro- cess begins with the recognition through Dicer-like ribonucleases （DCL） of double-stranded RNA （dsRNA） that is generated during viral replication. Upon recognition, the dsRNA is processed into 21-24 nucleotide fragments, termed small interfering RNA （siRNA）. Based on sequence homology, both viral siRNA and plant mRNA associate with an RNA-induced silencing complex （RISC） and are targeted for degradation （Llave, 2010）.

关键词： TOBACCO RATTLE VIRUS NICOTIANA-BENTHAMIANA PLANTS

Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin

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Horticulture Research 2016年第1期3卷 105-116页

作者： Lindsay E Wyatt Susan R Strickler lukas a mueller Michael Mazourek School of Integrative Plant Sciences Section of Plant Breeding and GeneticsCornell UniversityIthacaNY 14850USA Boyce Thompson Institute for Plant Research IthacaNY 14853USA Johnny’s Selected Seeds 255 Benton AvenueWinslowMaine 04901USA

Both the fruit mesocarp and the seeds of winter squash can be used for consumption,although the focus of breeding efforts varies by *** bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content,percent dry matter,and percent soluble solids,while these traits are essentially ignored in oilseed *** compare fruit development in these two types of squash,we sequenced the fruit transcriptome of two cultivars bred for different purposes:an acorn squash,‘Sweet REBA’,and an oilseed pumpkin,‘Lady Godiva’.Putative metabolic pathways were developed for carotenoid,starch,and sucrose synthesis in winter squash fruit and squash homologs were identified for each of the structural genes in the *** expression,especially of known rate-limiting and branch point genes,corresponded with metabolite accumulation both across development and between the two ***,developmental regulation of metabolite genes is an important factor in winter squash fruit quality.

关键词： cultivar squash breeding

An acorn squash (Cucurbita pepo ssp. ovifera) fruit and seed transcriptome as a resource for the study of fruit traits in Cucurbita

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Horticulture Research 2015年第1期2卷 310-316页

作者： Lindsay E Wyatt Susan R Strickler lukas a mueller Michael Mazourek Department of Plant Breeding and Genetics Cornell UniversityIthacaNYUSA Boyce Thompson Institute for Plant Research Tower RoadIthacaNYUSA

Acorn squash(Cucurbita pepo)is an iconic fall vegetable in the United States,known for its unique fruit shape and also prized for its culinary *** is known about the metabolism that underlies the development of fruit quality attributes such as color,sweetness,texture and nutritional qualities in acorn squash,or any other winter squash grown *** provide insight into winter squash fruit and seed development and add to the genomic resources in the Cucurbita genus,RNA sequencing was used to generate an acorn squash fruit and seed transcriptome from the cultivar Sweet REBA at critical points throughout fruit development.141838600 high-quality paired-end Illumina reads were assembled into 55949 unigenes.85%of unigenes with predicted open reading frames had homology with previously identified genes and over 62%could be functionally *** with the watermelon and cucumber genomes provided confirmation that the unigenes are full-length and comprehensive,covering an average of 90%of the coding sequence of their homologs and 72%of the cucumber and watermelon *** candidate genes associated with carotenoid and carbohydrate metabolism were identified toward a resource for winter squash fruit quality trait *** transcriptome represents a major advance in *** genomics,providing significant new sequence information and revealing the repertoire of genes expressed throughout winter squash fruit and seed *** studies on the genetic basis of fruit quality and future breeding efforts will be enhanced by tools and insights developed from this resource.

关键词： squash breeding cultivar

Exploiting the diversity of tomato:the development of a phenotypically and genetically detailed germplasm collection

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Horticulture Research 2020年第1期7卷 1941-1954页

作者： Estefanía Mata-Nicolás Javier Montero-Pau Esther Gimeno-Paez Víctor Garcia-Carpintero Peio Ziarsolo Naama Menda lukas A.mueller JoséBlanca Joaquín Cañizares Esther van der Knaap María JoséDíez Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana.COMAV.Universitat Politècnica de València ValenciaSpain Department of Biochemistry and Molecular Biology Universitat de ValènciaValenciaSpain Boyce Thompson Institute IthacaNYUSA Institute of Plant Breeding Genetics and GenomicsUniversity of GeorgiaGeorgiaGAUSA Department of Horticulture University of GeorgiaGeorgiaGAUSA

A collection of 163 accessions,including Solanum pimpinellifolium,Solanum lycopersicum *** and Solanum lycopersicum ***,was selected to represent the genetic and morphological variability of tomato at its centers of origin and domestication:Andean regions of Peru and Ecuador and *** collection is enriched with *** *** from the Amazonian region that has not been analyzed previously nor used *** collection has been morphologically characterized showing diversity for fruit,flower and vegetative *** genomes were sequenced in the Varitome project and are publicly available(***/projects/varitome).The identified SNPs have been annotated with respect to their impact and a total number of 37,974 out of 19,364,146 SNPs have been described as high impact by the SnpEeff *** has shown associations for different traits,demonstrating the potential of this collection for this kind of *** have not only identified known QTLs and genes,but also new regions associated with traits such as fruit color,number of flowers per inflorescence or inflorescence *** speed up and facilitate the use of this information,F2 populations were constructed by crossing the whole collection with three different *** F2 collection is useful for testing SNPs identified by GWAs,selection sweeps or any other candidate *** data is available on Solanaceae Genomics Network and the accession and F2 seeds are freely available at COMAV and at TGRC *** these resources together make this collection a good candidate for genetic studies.

关键词： germplasm collection traits

西瓜基因组框架图谱绘制与20份代表性资源重测序研究

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中国瓜菜 2019年第8期32卷 193-195页

作者：郭绍贵(编译) 许勇(编译) 张建国孙宏贺 Jerome Salse William JLucas 张海英 Yi Zheng 毛林勇任毅王志文闵久梦郭晓森 Florent Murat Byung-Kook Ham 张召亮高山黄明云徐益民钟思林 Aureliano Bombarely lukas a mueller 赵泓何洪巨张岩张忠华黄三文谭涛庞尔丽林魁胡群匡汉辉倪培相王博刘景安孔秋生侯文菊邹小花江姣宫国义 Kathrin Klee Heiko Schoof 黄莹胡雪松董珊珊梁德全王娟吴奎夏阳赵翔郑泽群邢淼梁新明黄邦清吕添王军一尹烨伊鸿平李瑞强吴明珠 Amnon Levil 张兴平 James J Giovannoni 王俊李云伏费章君北京市农林科学院蔬菜研究中心国家蔬菜工程技术研究中心·北京市农林科学院·农业部华北地区园艺作物生物学与种质创制重点实验室康奈尔大学博伊斯汤普逊研究所农业部基因组学重点实验室·深圳华大基因研究院北京农学院植物科学技术学院法国农业科学研究院联合研究中心谷类遗传学多样性和生态生理研究所加州大学生物科学学院植物生物学系(戴维斯) 中国农业科学院蔬菜花卉研究所北京师范大学生命科学学院华中农业大学园林学院波恩大学植物科学与资源保护研究所作物生物信息研究室新疆农业科学院北京诺禾致源生物信息科技有限公司美国农业部农业科学研究院蔬菜研究室美国农业部农业与健康罗伯特霍利研究中心哥本哈根大学生物系复旦大学理论生命科学研究中心

目的与意义:西瓜是一种重要的葫芦科作物,占全世界蔬菜生产面积的7%.经过长期人工选育,西瓜遗传多样性变的十分狭窄,已成为西瓜品种改良的瓶颈.本研究以东亚类型高品质西瓜品种‘97103’为材料进行西瓜全基因组序列分析,并开展20份代表... 详细信息

目的与意义:西瓜是一种重要的葫芦科作物,占全世界蔬菜生产面积的7%.经过长期人工选育,西瓜遗传多样性变的十分狭窄,已成为西瓜品种改良的瓶颈.本研究以东亚类型高品质西瓜品种‘97103’为材料进行西瓜全基因组序列分析,并开展20份代表性西瓜资源的基因组重测序分析,以明确西瓜基因组结构和进化历程,阐明果实品质和维管束信号传导等重要生物学过程的分子机制.

关键词：全基因组测序分析西瓜资源图谱框架品种改良果实品质