The SGN VIGS Tool： User-Friendly Software to Design Virus-Induced Gene Silencing （VIGS） Constructs for Functional Genomics

作     者：Noe Femandez-Pozo Heman G. Rosli Gregory B. Martin Lukas A. Mueller 

作者机构：Boyce Thompson Institute for Plant Research Ithaca NY 14853 USA Instituto de Investigaciones Biotecnolbgicas - Instituto Tecnológico de Chascomús (IIB-INTECH) UNSAM-CONICET Chascomús B71301WA Buenos Aires Argentina Section of Plant Pathology and Plant-Microbe Biology School of Integrative Plant Science Cornell University Ithaca NY 14853 USA 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2015年第8卷第3期

页      面：486-488页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported  in part  by National Science Foundation (GBM) 

主　　题：@@ 

摘      要：Dear Editor, Virus-induced gene silencing （VIGS） is a fast and powerful method to study gene function in plants （Burch-Smith et al., 2004）. It is based on plant defense mechanisms against viral gene replication and allows high-throughput silencing of genes of inter- est （SenthiI-Kumar and Mysore, 2014）. The molecular mechanisms involved in post-transcriptional gene silencing （PTGS） have been studied intensively, and its steps are well known. The silencing pro- cess begins with the recognition through Dicer-like ribonucleases （DCL） of double-stranded RNA （dsRNA） that is generated during viral replication. Upon recognition, the dsRNA is processed into 21-24 nucleotide fragments, termed small interfering RNA （siRNA）. Based on sequence homology, both viral siRNA and plant mRNA associate with an RNA-induced silencing complex （RISC） and are targeted for degradation （Llave, 2010）.