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TCF7L2 rs7903146 polymorphism is associated with gastric cancer: a case-control study in the Venezuelan population

World Journal of Gastroenterology 2016年第28期22卷 6520-6526页

作者： Keila Torres luis labrador Elvis Valderrama Miguel Angel Chiurillo Laboratorio de Genetica Molecular 'Dr. Jorge Yunis-Turbay' Decanato de Ciencias de la Salud Universidad Centroccidental Lisandro Alvarado Barquisimeto 3001 Venezuela Postgrado en Ciencias Biologicas Departamento de Biologia Celular Universidad Simon Bolivar Caracas 1081-A Venezuela Departamento de Anatomia Patologia Hospital Antonio Maria Pineda-UCLA Barquisimeto 3001 Venezuela Departamento de Patologia Clinica Universidade Estadual de Campinas Campinas 13083 Sao Paulo Brazil

AIM: To explore the association between TCF7L2 rs12255372 and rs7903146 single nucleotide polymorphisms (SNPs) and gastric cancer risk in Venezuelan ***: We performed a case-control study including 122 paraffin-embedded archived intestinal-type gastric cancer samples and 129 biopsies obtained by superior endoscopy from chronic gastritis patients. Gastric cancer samples were classified according the degree of carcinoma differentiation. Genomic DNA was extracted from tissues, and the two SNPs of TCF7L2 gene (rs12255372 and rs7903146) were genotyped by polymerase chain reaction-restriction fragment length polymorphism reactions. Multiple regression analysis with adjustments for age and gender were performed and best-fitting models of inheritance were determined. Statistic powers were post-hoc ***: After adjusting for age and sex the TCF7L2 rs7903146 TT genotype was associated with gastric cancer risk under the recessive genetic model (OR = 3.11, 95%CI: 1.22-7.92, P = 0.017). We further investigated the distribution of rs12255372 and rs7903146 genotypes according gastric cancer stratified by degree of differentiation, and we observed that carriers of rs7903146 T allele (CT + TT vs CC) had a significantly increased risk of moderate/well differentiated gastric cancer (dominant model, OR = 2.55, 95%CI: 1.35-4.80, P = 0.004), whereas the rs7903146 TT genotype was associated with poorly differentiated gastric cancer in the recessive model (OR = 3.65, 95%CI: 1.25-10.62, P = 0.018). We did not find association between rs12255372 SNP and the susceptibility of developing gastric ***: TCF7L2 rs7903146 polymorphism is associated with gastric cancer risk in the Venezuelan population, and could be related to determine the degree of differentiation of tumor cells.

关键词： Gastric cancer Wnt/β -catenin pathway TCF7L2 Single nucleotide polymorphism Genetic susceptibility

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Neuroprotective effects of G9a inhibition through modulation of peroxisome-proliferator activator receptor gamma-dependent pathways by miR-128

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Neural Regeneration Research 2024年第11期19卷 2532-2542页

作者： Aina Bellver-Sanchis Pedro AAvila-López Iva Tic David Valle-García Marta Ribalta-Vilella luis labrador Deb Ranjan Banerjee Ana Guerrero Gemma Casadesus Coralie Poulard Mercè Pallàs Christian Grinán-Ferré Department of Pharmacology and Therapeutic Chemistry Institut de Neurociències-Universitat de BarcelonaBarcelonaSpain Department of Biochemistry and Molecular Genetics Feinberg School of MedicineNorthwestern UniversityChicagoILUSA Institute of Biotechnology National Autonomous University of MexicoCuernavacaMexico Department of Pharmacology and Therapeutics Health Science Center-University of FloridaGainesvilleFLUSA Department of Chemistry National Institute of Technology DurgapurM G AvenueDurgapurWest BengalIndia Cancer Research Cancer Lyon Universitéde LyonLyonFrance Inserm U1052 Centre de Recherche en Cancérologie de LyonLyonFrance CNRS UMR5286 Centre de Recherche en Cancérlogie de LyonLyonFrance Centro de Investigación en Red Enfermedades Neurodegenerativas(CIBERNED)Instituto de Salud Carlos IIIMadridSpain Neuroimmunology Department National Institute of Neurology and Neurosurgery“Manuel Velasco Suárez”Mexico CityMexico

Dysregulation of G9a,a histone-lysine N-methyltransferase,has been observed in Alzheimer’s disease and has been correlated with increased levels of chronic inflammation and oxidative ***,microRNAs are involved in many biological processes and diseases playing a key role in pathogenesis,especially in multifactorial diseases such as Alzheimer’s ***,our aim has been to provide partial insights into the interconnection between G9a,microRNAs,oxidative stress,and *** better understand the biology of G9a,we compared the global microRNA expression between senescence-accelerated mouse-prone 8(SAMP8)control mice and SAMP8 treated with G9a inhibitor *** found a downregulation of miR-128 after a G9a inhibition treatment,which interestingly binds to the 3′untranslated region(3′-UTR)of peroxisome-proliferator activator receptor γ(PPARG)***,Pparg gene expression levels were higher in the SAMP8 group treated with G9a inhibitor than in the SAMP8 control *** also observed modulation of oxidative stress responses might be mainly driven Pparg after G9a *** confirm these antioxidant effects,we treated primary neuron cell cultures with hydrogen peroxide as an oxidative *** this setting,treatment with G9a inhibitor increases both cell survival and antioxidant ***,up-regulation of PPARγby G9a inhibitor could also increase the expression of genes involved in DNA damage responses and *** addition,we also described that the PPARγ/AMPK axis partially explains the regulation of autophagy markers ***,PPARγ/GADD45αpotentially contributes to enhancing synaptic plasticity and neurogenesis after G9a ***,we propose that pharmacological inhibition of G9a leads to a neuroprotective effect that could be due,at least in part,by the modulation of PPARγ-dependent pathways by miR-128.

关键词： aging cognitive decline epigenetics G9a inhibition microRNAs miR-128 peroxisome-proliferator activator receptorγ(PPARγ) PPARG SAMP8

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Diagnosis of Mycobacterium tuberculosis using molecular biology technology

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Asian Pacific Journal of Tropical Biomedicine 2011年第2期1卷 89-93页

作者： Juan Garberi Jorge labrador Federico Garberi Juan Ezequiel Garberi Julian Peneipil Miguel Garberi luis Scigliano Alcides Troncoso Laboratory of Molecular Biology and Pathology School of MedicineBuenos Aires University Orange Solutions School of MedicineBuenos Aires University Department of Infectious Diseases School of MedicineBuenos Aires University

Objective:To present an integrated molecular biology dedicated system for tuberculosis ***:One hundred and five sputum specimens from patients strongly suspected by clinical parameters of tuberculosis were studied by Ziehl-Neelsen staining,by cultivation on solid medium and by a balanced hemincsted fluorometric PCR system(Orange C3TB) that could preserve worker safety and produce a rather pure material free of potential inhibitors. DNA amplification was performed in a low cost tuberculosis *** double stranded DNA was flurometrically *** whole reaction was conducted in one single tube which would not be opened after adding the processed sample in order to minimize the risk of cross contamination with ***:The assay was able to delect 30 bacillus per sample mL with 99.8%interassay variation *** was positive in 23(21.9%) tested samples(21 of them were smear negative).In our study it showed a preliminary sensitivity of 94.5%for sputum and an overall specificity of 98.7%.Conclusions:Total run time of the test is 4 h with 2.5 real working *** PCR positive samples are also positive by microbiological culture and clinical *** show that it could be a very useful tool to increase detection efficiency of tuberculosis disease in low bacilus load ***,its low cost and friendly using make it feasible to run in poor regions.

关键词： Tuberculosis Sputum Molecular diagnosis Low cost Real-time PCR Mycobacterium tuberculosis Molecular biology technology Sputum sample Microbiological culture

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Diagnostic-therapeutic management of bile duct cancer

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World Journal of Clinical Cases 2019年第14期7卷 1732-1752页

作者： José María Huguet Miriam Lobo José Mir labrador Carlos Boix Cecilia Albert luis Ferrer-Barceló Ana B Durá Patricia Suárez Isabel Iranzo Mireia Gil-Raga Celia Baez de Burgos Javier Sempere Digestive Disease Department General University Hospital of ValenciaValencia 46014Spain Medical Oncology Department General University Hospital of ValenciaValencia 46014Spain Unidad Hepática Avanzada Servicio de Cirugía General y DigestivaGeneral University Hospital of ValenciaValencia 46014Spain Medical Oncology Department Hospital de RequenaRequena 46340Spain

Biliary tract cancer, or cholangiocarcinoma, comprises a heterogeneous group of malignant tumors that can emerge at any part of the biliary tree. This group is the second most common type of primary liver cancer. Diagnosis is usually based on symptoms, which may be heterogeneous, and nonspecific biomarkers in serum and biopsy specimens, as well as on imaging techniques. Endoscopy-based diagnosis is essential, since it enables biopsy specimens to be taken. In addition, it can help with locoregional staging of distal tumors. Endoscopic retrograde cholangiopancreatography is a key technique for the evaluation and treatment of malignant biliary tumors. Correct staging of cholangiocarcinoma is essential in order to be able to determine the degree of resectability and assess the results of treatment. The tumor is staged based on the TNM classification of the American Joint Committee on Cancer. The approach will depend on the classification of the tumor. Thus, some patients with early-stage disease could benefit from surgery;complete surgical resection is the cornerstone of cure. However, only a minority of patients are diagnosed in the early stages and are suitable candidates for resection. In the subset of patients diagnosed with locally advanced or metastatic disease, chemotherapy has been used to improve outcome and to delay tumor progression. The approach to biliary tract tumors should be multidisciplinary, involving experienced endoscopists, oncologists, radiologists, and surgeons.

关键词： Bile duct cancer Cholangiocarcinoma Management Diagnosis Incidence Multidisciplinary treatment

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