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Haplotype phasing after joint estimation of recombination and linkage disequilibrium in breeding populations

Journal of Animal Science and Biotechnology 2014年第1期5卷 38-52页

作者： luis gomez-Raya Amanda M Hulse David Thain Wendy M Rauw Departamento de Mejora Genética Animal Instituto Nacional deInvestigación y Tecnología Agraria y Alimentaria (INIA) Interdisciplinary Program in Genetics Texas A&M University College Station Department of Animal Biotechnology University of Nevada

A novel method for haplotype phasing in families after joint estimation of recombination fraction and linkage disequilibrium is developed. Results from Monte Carlo computer simulations show that the newly developed E.M. algorithm is accurate if true recombination fraction is 0 even for single families of relatively small sizes. Estimates of recombination fraction and linkage disequilibrium were 0.00 （SD 0.00） and 0.19 （SD 0.03） for simulated recombination fraction and linkage disequilibrium of 0.00 and 0.20, respectively. A genome fragmentation phasing strategy was developed and used for phasing haplotypes in a sire and 36 progeny using the 50 k Illumina BeadChip by： a） estimation of the recombination fraction and LD in consecutive SNPs using family information, b） linkage analyses between fragments, c） phasing of haplotypes in parents and progeny and in following generations. Homozygous SNPs in progeny allowed determination of paternal fragment inheritance, and deduction of SNP sequence information of haplotypes from dams. The strategy also allowed detection of genotyping errors. A total of 613 recombination events were detected after linkage analysis was carried out between fragments. Hot and cold spots were identified at the individual （sire level）. SNPs for which the sire and calf were heterozygotes became informative （over 90%） after the phasing of haplotypes. Average of regions of identity between half-sibs when comparing its maternal inherited haplotypes （with at least 20 SNP） in common was 0.11 with a maximum of 0.29 and a minimum of 0.05. A Monte-Carlo simulation of BTA1 with the same linkage disequilibrium structure and genetic linkage as the cattle family yielded a 99.98 and 99.94% of correct phases for informative SNPs in sire and calves, respectively.

关键词： Breeding Haplotype phasing Linkage disequilibrium SNP

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A Regulatory Module Controlling CA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis

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Molecular Plant 2019年第1期12卷 71-85页

作者： Rocio Sanchez-Montesino Laura Bouza-Morcillo Julietta Marquez Melania Ghita Salva Duran-Nebreda luis gomez Michael J.Holdsworth George Bassel luis Onate-Sanchez Centro de Biotecnologia y Genomica de Plantas Universidad Politecnica de Madrid-lnstituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria Campus de MontegancedoPozuelo de AlarconMaddd 28223Spain School of Biosciences University of NottinghamLoughborough LE12 5RDUK School of Biosciences University of BirminghamBirmingham B15 23-rUK Departamento de Sistemas y Recursos Naturales E.T.S.I.MontesForestal y del Medio NaturalCampus de Moncloa28040 MadridSpain

A key component of seed germination is the interplay of mechanical forces governing embryo growth and the surrounding restraining endosperm *** cell separation is therefore thought to play a critical role in the control of this developmental *** we demonstrate that in Arabidopsis thaliana seeds,endosperm cell expansion is a key component of *** cells expand to accommodate embryo growth prior to *** show that this is an actively regulated process supported by spatiotemporal control of the cell expansion gene EXPANSIN 2 (EXPA2).The NAC transcription factors NAC25 and NACIL were identified as upstream regulators of EXPA2 expression,gibbereliinmediated endosperm expansion,and seed *** DELLA protein RGL2 repressed activation of the EXPA2 promoter by NAC25/*** together,our findings uncover a key role of the GA/ DELLA-NAC25/NACIL-EXPA2 network in regulating endosperm cell expansion to control the seed-toseedling transition.

关键词： cell expansion cell-wall remodeling enzymes endosperm expansin NAC transcription factors seed germination

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Effect of synthesis variables of plasma synthesized polymers on growth of HepG2 cells

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BIOCELL 2017年第2期41卷 41-43页

作者： ELIZABETH PEREZ-TEJADA JUAN MORALES-CORONA luis ERNESTO gomez-QUIROZ MARIA CONCEPCIONGUTIERREZ-RUIZ ROBERTO OLAYO Departamento de Química.Universidad Autónoma Metropolitana Unidad IztapalapaApdo.Postal 55-534IztapalapaCDMXCP 09340 Departamento de Ciencias de la Salud.Universidad Autónoma Metropolitana Unidad IztapalapaApdo.Postal 55-534IztapalapaCDMXCP 09340 Departamento de Física.Universidad Autónoma Metropolitana Unidad IztapalapaApdo.Postal 55-534IztapalapaMéxicoD.F.CP 09340 CECyT No.8 Instituto Politécnico NacionalAve.de las Granjas 618Jardín AzpeitiaAzcapotzalcoCDMXCP 02530

Low pressure plasma polymer films were synthesized using pyrrole and allylamine monomers and adding iodine was used(or not)for the reaction in both *** were polymerized on glass substrates under the same reaction *** of allylamine was also studied at different operating *** thin polymer films were used as culture surfaces for HepG2 cells,a cell line derived from a human *** proliferation,differentiation and two-dimensional propagation until obtaining monolayer of the cells was studied on the different synthetized films and correlations were established between the conditions of synthesis,the physicochemical characteristics obtained and the performance as substrates for the cellular growth.

关键词： Hepatocytes Pyrrole Allylamine Plasma Polymerization

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Statins and endocrine resistance in breast cancer

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Cancer Drug Resistance 2021年第2期4卷 356-364页

作者： Tara Hyder Juan luis gomez Marti Azadeh Nasrazadani Adam M.Brufsky University of Pittsburgh Physicians PittsburghPA 15213USA Department of Pathology University of Pittsburgh School of MedicinePittsburghPA 15213USA UPMC Hillman Cancer Center Magee Women's HospitalPittsburghPA 15213USA.

Most breast cancers are hormone-receptor positive(HR+).However,more women eventually die from HR+breast cancer than from either HER2+or triple negative breast *** therapies continue to be the mainstay of *** 40%of these cases,recurrences in early-stage disease and progression in the metastatic setting are largely a function of the development of endocrine resistance.A multitude of mediators and pathways have been associated with endocrine resistance in breast cancer including the mevalonate pathway,which is integral to cholesterol *** mevalonate pathway and the downstream activation of associated cytoplasmic pathways including PI3K-AKT-mTOR and RAS-MEK-ERK have been known to affect cancer cell proliferation,cell survival,cell invasion,and *** are important mechanisms leading to the inevitable development of endocrine resistance in HR+breast *** are a class of drugs that inhibits HMG-CoA reductase,an enzyme in the mevalonate pathway that plays a central role in cholesterol *** vitro and in vitro studies suggest that the role of statins in blocking the mevalonate pathway effectively disrupts downstream pathways involved in estrogen receptor expression and cellular processes such as cell survival,proliferation,stress,cell cycle,inhibition of apoptosis,and *** these key mechanisms heralds a role for statins in the prevention of endocrine resistance.

关键词： Statin HMGCR endocrine resistance autophagy PI3K mTOR

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Improvement of the Human Resource Management of Service Enterprises in Maturity Phrase-Implementing the Balance Scorecard Tool

Improvement of the Human Resource Management of Service Ente...

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作者：沙路（luis Chavez gomez）海南大学

学位级别：硕士

公司的生命周期是可以划分阶段的,在每个公司的生命周期中,每个阶段都没有一个持续时间或显式点的到来,它涉及公司的目标和价值观的定义及其行为者。在成熟阶段,公司应该担心的激励和保留员工,试图延长公司寿命以及降低离职率。这将在... 详细信息

公司的生命周期是可以划分阶段的,在每个公司的生命周期中,每个阶段都没有一个持续时间或显式点的到来,它涉及公司的目标和价值观的定义及其行为者。在成熟阶段,公司应该担心的激励和保留员工,试图延长公司寿命以及降低离职率。这将在本文的假说的基础。具体而言,一方面,人力资本微分方程将表示所有者期望在长期稳定和组织的成功。另一方面,公司还应该有助于降低成本,以及提高收入,并实现深入分析,而且可以通过设立研究创新商业活动的方式的跨学科小组的内部流程进行改进。本研究打算分析和测量公司在成熟阶段,通过使用平衡计分卡,研究影响人力资本服务公司的人力资源管理实践,以确定公司本身的运行效率。研究普兰和诺顿最初提出的相互影响的子系统。通过面向人力资源领域的研究,使用平衡计分卡进行分析。结果显示一家公司中员工成为很重要的资产。我们使用一种方法,使我们能够改善人力资源管理,确定人力资源领域有子系统帮助公司确定量化指标和定性的补充报告。平衡计分卡被证明是一个非常有用的来评价组织绩效的工具,使我们能够整合人力资源,有更广泛的角度来看待人力资源管理实践对员工绩效子系统的影响。

关键词：平衡记分卡员工绩效效率人力资源管理

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Optimization of Micromechanical Cleavage Technique of Natural Graphite by Chemical Treatment

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Graphene 2014年第1期3卷 1-5页

作者： luis Torres luis gomez Armas Antonio Carlos Seabra Centro de Ciências Naturais e Humanas Universidade Federal do ABC Sao Paulo Brazil Grupo de óptica Micro e Nanofabricao de Dispositivos (GOMNDI) Universidade Federal do Pampa Rio Grande do Sul Brazil Laboratório de Sistemas Integráveis do Departamento de Engenharia de Sistemas Eletronicos da Escola Politécnica da USP (LSI-PSI/EPUSP) Sao Paulo Brazil

In this work,we report a method to improve the efficiency of the micromechanical cleavage technique to obtain few-layers graphene samples, from natural graphite flakes, which were previously submitted to two chemical treatment times with H2SO4(17 and 25 hours). After the chemical treatment times, Raman spectroscopy reveals a hydrogenation of the few-layer graphene samples, which were obtained from the treated graphite flakes. To analyze the hydrogenation of the samples, the G and 2D bands of the Raman spectra of the treated and un-treated samples were analyzed and compared, as well as the I(2D)/I(G) ratio, revealing a p-doping on the treated samples when compared with the untreated samples. Our studies could be of great importance to obtain larger and greater amount of few-layer graphene samples.

关键词： Graphene Micromechanical Cleavage Raman Spectroscopy Chemical Treatment

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Evaluation of activity of triclabendazole against Taenia solium metacestode in naturally infected pigs

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Asian Pacific Journal of Tropical Medicine 2016年第1期9卷 20-23页

作者： Ana Vargas-Calla luis A.gomez-Puerta Juan Calcina Omar Gonzales-Viera Cesar Gavidia Maria T.Lopez-Urbina Hector H.Garcia Armando E.Gonzalez School of Veterinary Medicine Universidad Nacional Mayor de San Marcos Cysticercosis Elimination Program and Center for Global Heath Tumbes Universidad Peruana Cayetano Heredia Department of Microbiology School of SciencesUniversidad Peruana Cayetano Heredia

Objective:To assess the efficacy of triclabendazole(TCBZ) in porcine ***:Eighteen naturally infected cysticercotic pigs were divided into 3 groups of 6 individuals *** first group was treated orally with TCBZ at a single dose of 30 mg/kg of body weight,the second group was treated orally with oxfendazole at a single dose of 30 mg/kg of body weight and the third group received a placebo(control group).All animals were kept under the same management *** pigs were euthanized 17 wk post-treatment and the number of surviving cysts in muscles was assessed and compared between ***:All pigs treated with oxfendazole had only degenerated cysts in their *** contrast,TCBZ had very little effect against the parasitic *** from pigs in the TCBZ group looked apparently normal after ***,histological evaluation showed a mild to moderate degree of ***:TCBZ is not an efficacious drug against Taenia solium cysticercosis in swine using a single dose.

关键词： Taenia solium Cysticercosis Triclabendazole Oxfendazole Treatment

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Oxfendazole as successful treatment of Taenia hydatigena metacestodes in naturally infected pigs

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Asian Pacific Journal of Tropical Biomedicine 2015年第11期5卷 971-973页

作者： luis Antonio gomez-Puerta Armando Emiliano Gonzalez Cesar Gavidia Viterbo Ayvar Hector Hugo Garcia Maria Teresa Lopez-Urbina for the Cysticercosis Working Group in Peru School of Veterinary Medicine National University of San Marcos Cysticercosis Elimination Program and Center for Global Health Tumbes Cayetano Heredia University Department of Microbiology School of SciencesCayetano Heredia University

The efficacy of oxfendazole(OFZ) on Taenia hydatigena metacestodes, also called Cysticercus tenuicollis(C. tenuicollis), was studied in 648 raising pigs. This study was performed in Tumbes Department in Peru, an endemic area for cysticercosis. Pigs were randomized in two groups; untreated group(n = 142) did not receive any treatment and treated group(n = 506) received OFZ treatment at a single dose of 30 mg/kg body *** months after treatment, the pigs were necropsied. The prevalence of infection by C. tenuicollis among the pigs was 27.5%(39/142) and 2.0%(10/506) in untreated and treated groups, respectively. Untreated group was infested only with viable cysts, whereas treated group had no viable cysts. All the cysts found in treated group presented degeneration, with a thick membrane, and they contained milky fluid and fibrous tissue. A single dose of OFZ was effective against C. tenuicollis, thus providing an alternative drug for controlling this parasite in pigs.

关键词： Taenia hydatigena Cysticercus tenuicollis Oxfendaz

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Culture Mixed-Sex and Monosex of Tilapia in Ponds in Mexico City

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Agricultural Sciences 2015年第2期6卷 187-194页

作者： Jose luis gomez-Márquez Bertha Pena-Mendoza Maria del Carmen Alejo-Plata Jose luis Guzmán-Santiago Laboratorio de Limnología Facultad de Estudios Superiores ZaragozaUniversidad Nacional Autónoma de MéxicoMéxicoD.F.México Campus Puerto Angel Universidad del MarCiudad UniversitariaPuertoángelMéxico

In this study, the growth of Oreochromis niloticus both mixed sex and males sexually reversed were analyzed in concrete ponds in the FES Zaragoza, UNAM, under the environmental conditions of the Mexico City. The experiment was carried from May to November 2013, in two concrete ponds of 50 m2. Similar feeding regime and stocking density of fish were maintained for the culture systems. Different growth parameters like body weight, length, daily weight gain (DWG), specific growth rate (SGR), feed conversion rate (FCR), Fulton’s condition index (K) and survival were analyzed during six months culture period. It was found that culture with monosex males tilapias yielded the highest weight, length, DWG and SGR compared to mixed sex populations tilapias. Water quality was monitored along the experiment. Based on the results, there were statistically significant differences in size (W = 20942.5, p 5 mg/L), warm water (>20°C), productive (3) and slightly basic (pH > 8). Acceptable growth of fish and a yield of 3 ton/ha/6 months were obtained in both two ponds;therefore, the culture of tilapia under conditions of Mexico City was recommended.

关键词： Tilapia Culture Growth Survival Mexico City

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Fretting Fatigue Evaluation Produced by A New Vertical Machine

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材料科学与工程（中英文版） 2010年第2期4卷 26-38页

作者： GuiUermo Urriolagoitia-Sosa Guillermo Urriolagoitia-Calderon luis Hector Hemaindez-gomez Beatriz Romero-Angeles Secci6n de Estudios de Posgrado e lnvestigacion Escuela Superior de lngenieria Mecanica y Electrica lnstituto Politecnico Nacional Unidad Profesional Adolfo Lopez Mateos 5th Building 2nd Floor Lindavista CP 07738 Gustavo A. Madero Mexico D F. Mexico.

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