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Characterization of oral yeasts isolated from healthy individuals attended in different Colombian dental clinics

The Journal of Biomedical Research 2019年第5期33卷 333-342页

作者： Raul Eduardo Rivera Alejandra Zuluaga Karen Arango Itzjak Kadar Paola Andrea Pinillos luis fernando montes Eugenia Catalina Cepeda Ernesto Gonzalez Pedro Antonio Alfonso Andrea Alejandra Villalba luis fernando Casanova Adolfo Perez Armando Roa Martha Jhoana Arias Jorge Orlando Francisco Cuellar Lorena Pedraza Adiel Alberto Vasquez Blanca Lynne Suarez Beatriz LGomez Catalina De Bedout Luz Elena Cano Group of Investigation in Oral Health Faculty of DentistryAntonio Narino UniversityArmeniaQuindio 630001Colombia Medical and Experimental MycologyUnit Corporation for Biological Research(CIB)MedellinAntioquia 050034Colombia School of Medicine and Health Sciences Universidad del RosarioCarreraBogota 111221Colombia School of Microbiology University of AntioquiaMedellinAntioquia 050036Colombia.

The aim of this study was to identify the most frequent yeasts in the oral cavity of adult individuals without immune disorders and to associate the presence of these oral yeasts with different characteristics of each individual. Oral rinse samples were obtained from 96 healthy adults and cultured in Sabouraud dextrose agar media and CHROMagar. Yeasts were identified by sequencing the D1/D2 region of the 28 S rRNA gene. Probable association among the socio-demographic characteristics, body mass index, family and personal medical history,oral hygiene, tobacco and/or alcohol consumption habits and presence of oral fungi was analyzed. Contingency tables and logistic regression were employed to evaluate possible relationships between the presence of oral fungi and mixed colonization with these variables. 57.3% of the healthy individuals had oral yeasts and 21.8% had mixed colonization. The most prevalent yeasts were Candida albicans(52%), C. parapsilosis(17.9%), and ***(7.57%). Yeasts with most frequently mixed colonization were C. albicans and C. parapsilosis. No relationships were found among the variables analyzed. However, the presence of mixed colonization was related to the presence of dental prostheses(P<0.006), dental apparatuses(P=0.016) and O’Leary index(P=0.012). This is the first study that characterized oral yeasts in Colombian healthy individuals, determined the most prevalent oral yeasts C. albicans, C. parapsilosis and C. dublinensis and an association of mixed colonization with the use of dental prostheses and aparatology and poor hygiene.

关键词： oral yeast Candida species microbial epidemiology

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Cover Crops as Affecting Soil Chemical and Physical Properties and Development of Upland Rice and Soybean Cultivated in Rotation

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Rice science 2018年第6期25卷 340-349页

作者： Adriano Stephan NASCENTE luis fernando STONE Embrapa Arroz e Feij(CNPAF) Rodovia GO-462km 12 Zona RuralCP.17975375-000Santo Antoio de GoiasGOBrasil

Cover crops can provide changes in soil chemical and physical properties, which could allow a sustainable development of soybean and upland rice rotation in Brazilian Cerrado. The objective of this study was to determine the effects of cover crops(cultivated in the offseason) in the soybean-upland rice rotation(cultivated in the summer season) on the soil chemical and physical properties, yield components and grain yield of the cash crops. The experimental design was a randomized block design in factorial scheme 4 × 2 with six replications. Treatments were composed by four cover crops: fallow, millet(Pennisetum glaucum) + Crotalaria ochroleuca, millet + pigeon pea(Cajanus cajans), and millet + pigeon pea + Urochola ruziziensis in the offseason with one or two cycles of cover crops, with rice(Oryza sativa)or soybean(Glycine max) in the summer season. Cover crops alone provided no changes in soil chemical properties. However, the rotation cover crops/cash crops/cover crops/cash crops reduced p H, Al and H + Al and increased Ca, Mg, K and Fe contents in the soil. The cover crops millet + pigeon pea and millet + pigeon pea + U. ruziziensis improved soil physical properties in relation to fallow,especially in the 0–0.10 m soil layer. In spite of the improvement of the soil physical properties after two years of rotation with cover crops and cash crops, the soil physical quality was still below the recommended level, showing values of macroporosity, S index and soil aeration capacity lower than 0.10 m3/m3, 0.035 and 0.34, respectively. Upland rice production was higher under mixtures of cover crops than under fallow, mainly because of soil physical changes done by these mixtures of cover *** grain yield was similar under all cover crops tested, but was higher after the rotation cover crops/upland rice/cover crops than after only one cycle of cover crops.

关键词： crop rotation no-tillage system sustainable agriculture tropical agriculture rice soybean

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Slope reliability and back analysis of failure with geotechnical parameters estimated using Bayesian inference

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Journal of Rock Mechanics and Geotechnical Engineering 2019年第3期11卷 628-643页

作者： luis-fernando Contreras Edwin T.Brown The University of Queensland St Lucia Campus

A Bayesian approach is proposed for the inference of the geotechnical parameters used in slope *** methodology involves the construction of posterior probability distributions that combine prior information on the parameter values with typical data from laboratory tests and site investigations used in *** posterior distributions are often complex,multidimensional functions whose analysis requires the use of Markov chain Monte Carlo(MCMC)*** procedures are used to draw representative samples of the parameters investigated,providing information on their best estimate values,variability and *** paper describes the methodology to define the posterior distributions of the input parameters for slope design and the use of these results for evaluation of the reliability of a slope with the first order reliability method(FORM).The reliability analysis corresponds to a forward stability analysis of the slope where the factor of safety(FS)is calculated with a surrogate model from the more likely values of the input *** Bayesian model is also used to update the estimation of the input parameters based on the back analysis of slope *** this case,the condition FS?1 is treated as a data point that is compared with the model prediction of *** analysis requires a sufficient number of observations of failure to outbalance the effect of the initial input *** parameters are updated according to their uncertainty,which is determined by the amount of data supporting *** methodology is illustrated with an example of a rock slope characterised with a Hoek-Brown rock mass *** example is used to highlight the advantages of using Bayesian methods for the slope reliability analysis and to show the effects of data support on the results of the updating process from back analysis of failure.

关键词： Bayesian analysis Hoek-Brown criterion Slope reliability Back analysis of failure

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Hidden in plain sight:Morphological and phylogenetic evidence for Bouteloua arizonica,a species distinct from Bouteloua aristidoides(Poaceae,Chloridoideae)

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Plant Diversity 2021年第2期43卷 125-133页

作者： luis fernando Cuellar-Garrido Maria Elena Siqueiros-Delgado Herbario Luz Maria Villarreal de Puga Del Instituto de Botanica(IBUG) Departamento de Botanica y ZoologíaCentro Universitario de Ciencias Biologicas y AgropecuariasUniversidad de GuadalajaraCamino Ing.Ramon Padilla Sanchez 2100CP 45200ZapopanJaliscoMexico Departamento de Biologia Centro de Ciencias BioasicasUniversidad Autonoma de AguascalientesAvenida Universidad 940CP 20131AguascalientesMexico

Two varieties of Bouteloua aristidoides have been recognized,the widespread *** and the more narrowly distributed *** two varieties differ in inflorescence form even more than that seen between many other closely related species of *** therefore asked whether these taxa might be better regarded as distinct species.A total of 93 vouchers were studied by using morphometry(principal components analysis and statistical tests),leaf micromorphology,ancestral state reconstruction,and/or molecular(ITS,trnC-rpoB and trnT-L-F)phylogenetic *** from the ITS tree,all results supported elevation of *** *** to the rank of species,thus the new combination Bouteloua arizonica(***)***&Columbus *** ***.,is *** and combined chloroplast-nuclear molecular trees depicted *** as monophyletic(even in sympatric populations with ***)and reveals phylogenetic structure within *** for which the presence of new undescribed varieties of ***(different from ***)is *** differs from *** in having fewer branches per inflorescence,a bigger branch with more spikelets,and a shorter branch *** electron microscopy revealed the presence of papillae on leaves of *** as a clear *** mature plants of *** from seeds in a greenhouse revealed a strong cleistogamous nature for this species for which gene flow in sympatric populations with *** seems unlikely.A taxonomic treatment and distribution map for identification of *** is provided.

关键词： Ancestral state reconstruction Bouteloua arizonica Cleistogamy Morphology Phylogeny Sympatry

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Bayesian data analysis to quantify the uncertainty of intact rock strength

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Journal of Rock Mechanics and Geotechnical Engineering 2018年第1期10卷 11-31页

作者： luis fernando Contreras Edwin T.Brown Marc Ruest The University of Queensland St Lucia CampusBrisbaneQLD 4072Australia Golder Associates Pty.Ltd. BrisbaneAustralia

One of the main difficulties in the geotechnical design process lies in dealing with uncertainty. Uncertainty is associated with natural variation of properties, and the imprecision and unpredictability caused by insufficient information on parameters or models. Probabilistic methods are normally used to quantify uncertainty. However, the frequentist approach commonly used for this purpose has some ***, it lacks a formal framework for incorporating knowledge not represented by data. Second, it has limitations in providing a proper measure of the confidence of parameters inferred from data. The Bayesian approach offers a better framework for treating uncertainty in geotechnical design. The advantages of the Bayesian approach for uncertainty quantification are highlighted in this paper with the Bayesian regression analysis of laboratory test data to infer the intact rock strength parameters σand mused in the Hoek-Brown strength criterion. Two case examples are used to illustrate different aspects of the Bayesian methodology and to contrast the approach with a frequentist approach represented by the nonlinear least squares(NLLS) method. The paper discusses the use of a Student’s t-distribution versus a normal distribution to handle outliers, the consideration of absolute versus relative residuals, and the comparison of quality of fitting results based on standard errors and Bayes factors. Uncertainty quantification with confidence and prediction intervals of the frequentist approach is compared with that based on scatter plots and bands of fitted envelopes of the Bayesian approach. Finally, the Bayesian method is extended to consider two improvements of the fitting analysis. The first is the case in which the Hoek-Brown parameter, a, is treated as a variable to improve the fitting in the triaxial region. The second is the incorporation of the uncertainty in the estimation of the direct tensile strength from Brazilian test results within the overall evaluati

关键词： Uncertainty Intact rock strength Bayesian analysis Hoek-Brown criterion

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Twenty years of population dynamics in European beech-oak forest at their rear range margin anticipate changes in its structure and composition

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Forest Ecosystems 2024年第3期11卷 358-370页

作者： álvaro Rubio-Cuadrado Iciar Alberdi Isabel Canellas fernando montes Jesús Rodríguez-Calcerrada Rosana López Guillermo G.Gordaliza María Valbuena-Carabana Nikos Nanos Ramón Perea luis Gil Departamento de dinamica y gestion forestal Instituto de Ciencias Forestales(ICIFOR-INIA)CSICCtra.La Coruna km 7.528040MadridSpain Departamento de Sistemas y Recursos Naturales Escuela Tecnica Superior de Ingeniería de MontesForestal y del Medio NaturalUniversidad Politecnica de Madrid.Ciudad Universitaria s/n28040MadridSpain School of Forestry and Natural Environment Aristotle University of ThessalonikiUniversity Campus54124ThessalonikiGreece

There is an increasing interest in restoring degraded forests,which occupy half of the forest *** the forms of restoration,passive restoration,which involves the elimination of degrading factors and the free evolution of natural dynamics by applying minimal or no management,is gaining *** dynamics is difficult to predict due to the influence of multiple interacting factors such as climatic and edaphic conditions,composition and abundance of species,and the successional character of these ***,we study the natural dynamics of a mixed forest located in central Spain,which maintained an open forest structure,due to intensive use,until grazing and cutting were banned in the *** most frequent woody species in this forest are Fagus sylvatica,Quercus petraea,Quercus pyrenaica,Ilex aquifolium,Sorbus aucuparia,Sorbus aria and Prunus avium,with contrasting shade and drought *** species are common in temperate European deciduous forest and are found here near their southern distribution limit,except for *** order to analyze forest dynamics and composition,three inventories were carried out in 1994,2005 and *** results show that,despite the Mediterranean influence,the natural dynamics of this forest has been mainly determined by different levels of shade *** the abandonment of grazing and cutting,*** expanded rapidly due to its lower shade tolerance,whereas after canopy closure and forest densification,shade-tolerant species gained ground,particularly ***,despite its lower drought and late-frost *** the current dynamics continue,*** will overtake the rest of the species,which will be relegated to sites with shallow soils and steep ***,all the multi-centennial beech trees,which are undergoing a rapid mortality and decline process,will disappear.

关键词： Forest succession Secondary forest Woodland Mixed forest Competition Recruitment

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Diversity of filamentous fungi in leaf litter and aerial litter in semideciduous forest,Bahia,Brazil

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Journal of Forestry Research 2015年第2期26卷 479-485页

作者： Marcos Fabio Oliveira Marques Edemir Barbosa dos Santos luis fernando Pascholati Gusmaao Laborato rio de Biologia Molecular de Fungos Universidade do Estado da BahiaCampus VIIBr 407Km 127Rod.Lomanto JuoniorSenhor do BonfimBA 44230-000Brazil Laboratorio de Micologia (LAMIC) Universidade Estadual de Feira de SantanaCaixa Postal 252Feira de SantanaBA 44036-900Brazil

The fungi have wide distribution in ecosystems and can be found colonizing various substrates,where they act as efficient decomposers,participating in cycling of *** present study aimed to assess the diversity,richness and composition of filamentous fungi in leaf litter and aerial litter in a semi-deciduous forest in the Chapada Diamantina,Bahia,*** of leaf litter and aerial litter were collected monthly from October/2009 to September/2010 in eight installments in the studied *** samples were washed,placed in moist chambers and incubated.47 taxa were identified,37 in leaf litter and 35 in aerial litter,with 25 species common to both.A similarity of 69 % according to Sorensen *** similarity was considered low when the sample points were compared to the same *** fungi showed sporadic intervals.

关键词： Plant debris Species richness Tropical microfungi

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Phosphorus fractions and sorption dynamics in the sediments of two Ca-SO4 water reservoirs in the central Argentine Andes

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International Journal of Sediment Research 2017年第3期32卷 442-451页

作者： José Gabriel León fernando luis Pedrozo Pedro Félix Temporetti Instituto Argentino de Nivología Glaciología y Ciencias Ambientales (IANIGLA)CCT Mendoza Departamento General de Irrigación Gobierno de Mendoza Espaa y Barcala Instituto de Investigaciones en Biodiversidad y Medio Ambiente (INIBIOMA) CONICET-Universidad Nacional del Comahue

Phosphorus（P） fractionation and sorption behavior were studied in the sediments of two calcium-sulfate(Ca-SO4: Ca2+170 mg L-1; SO42-400 mg L-1) water reservoirs in the southern central Argentine Andes-El Carrizal Reservoir（ECR）,which stratifies in summer and El Nihuil Reservoir（ENR）,which is vertically mixed throughout the year. Sediment size classes reflected the lithology of both basins and the reservoirs relative location: ECR（downstream from a valley adjacent to a mountain environment） had higher clay proportions while the sand fraction was more important in ENR（just next to a mountain environment）.In both reservoirs, the chemical composition revealed low alteration and calcium enrichment. Total P content was relatively high(1.1-1.6 mg P kg-1dry weight) as expected from the contribution of marine formations. P fractionation was dominated by Ca-P（ECR, 69% and ENR, 63%） followed by organic-P（23%）in summer-anoxic ECR and by iron/aluminum（Fe/Al）-P（27%） in oxic ENR. Batch experiments showed that sorption behavior in ECR had a typical Langmuir isotherm plot while in ENR that model fitted only at low dissolved P values(<5 mg PL-1) and co-precipitation evidence was observed at higher P concentrations. It is proposed that lower Fe/Al-P content in anoxic（ECR） vs. oxic（ENR） sediments results from the P released from iron-bound phosphorus due to sulfate reduction-ferrous sulfide formation in an anoxic environment. This condition should enable ECR sediments to adsorb P into fresh oxidized iron,and ENR sediments to co-precipitate P with calcium due to Fe/Al-P saturation, as observed in batch experiments. This paper constitutes the first description of phosphorus content and dynamics in reservoir sediments in arid central western Argentina, where water sulfate concentrations are naturally high due to basin lithology.

关键词： Reservoir Internal load Phosphorus binding Calcareous watershed Southern Central Andes

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New gene therapy strategies for hepatic fibrosis

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World Journal of Gastroenterology 2015年第13期21卷 3813-3825页

作者： Adriana M Salazar-montes luis D Hernández-Ortega Martha S Lucano-Landeros Juan Armendariz-Borunda Department of Molecular Biology and Genomics Institute for Molecular Biology and Gene TherapyUniversity of GuadalajaraGuadalajaraJalisco 44281México

The liver is the largest internal organ of the body, which may suffer acute or chronic injury induced by many factors, leading to cirrhosis and hepatocarcinoma. Cirrhosis is the irreversible end result of fibrous scarring and hepatocellular regeneration, characterized by diffuse disorganization of the normal hepatic structure, regenerative nodules and fibrotic tissue. Cirrhosis is associated with a high co-morbidity and mortality without effective treatment, and much research has been aimed at developing new therapeutic strategies to guarantee recovery. Liver-based gene therapy has been used to downregulate specific genes, to block the expression of deleterious genes, to delivery therapeutic genes, to prevent allograft rejection and to augment liver regeneration. Viral and non-viral vectors have been used, with viral vectors proving to be more efficient. This review provides an overview of the main strategies used in liver-gene therapy represented by non-viral vectors, viral vectors, novel administration methods like hydrodynamic injection, hybrids of two viral vectors and blocking molecules, with the hope of translating findings from the laboratory to the patient′s bed-side.

关键词： Gene therapy Hepatic fibrosis Viral vectors Non-vi

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Pressure drop analysis on the positive half-cell of a cerium redox flow battery using computational fluid dynamics: mathematical and modelling aspects of porous media

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Frontiers of Chemical Science and Engineering 2021年第2期15卷 399-409,F0004页

作者： fernando F.Rivera Berenice Miranda-Alcantara German Orozco Carlos Ponce de Leon luis F.Arenas National Council of Science and Technology(CONACYT) Mexico City 03940Mexico Center of Research and Technological Development in Electrochemistry(CIDETEQ) Queretaro 76703Mexico Electrochemical Engineering Laboratory Energy Technology Research GroupFaculty of Engineering and Physical SciencesUniversity of SouthamptonSouthampton SO171BJUK

Description of electrolyte fluid dynamics in the electrode compartments by mathematical models can be a powerful tool in the development of redox flow batteries(RFBs)and other electrochemical *** order to determine their predictive capability,turbulent Reynolds-averaged Navier-Stokes(RANS)and free flow plus porous media(Brinkman)models were applied to compute local fluid velocities taking place in a rectangular channel electrochemical flow cell used as the positive half-cell of a cerium-based RFB for laboratory *** different platinized titanium electrodes were considered,a plate plus a turbulence promoter and an expanded metal *** pressure drop was validated against experimental data obtained with typical cerium *** was found that the pressure drop values were better described by the RANS approach,whereas the validity of Brinkman equations was strongly dependent on porosity and permeability values of the porous media.

关键词： CFD simulation porous media porous electrode pressure drop redox flow battery

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