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Weighing of risk factors for penetrating keratoplasty graft failure：application of Risk Score System

International Journal of Ophthalmology(English edition) 2017年第3期10卷 372-377页

作者： Abdo Karim Tourkmani Valeria Sánchez-Huerta guillermo De Wit Jaime D.Martínez David Mingo Ignacio Mahillo-Fernández Ignacio Jiménez-Alfaro Asociación Para Evitar la Ceguera en México Hospital Luis Sáinchez Bulnes Mexico City 04030 México Hospital Fundación Jiménez Diaz Madrid 28040 Spain

AIM：To analyze the relationship between the score obtained in the Risk Score System（RSS）proposed by Hicks et al with penetrating keratoplasty（PKP）graft failure at 1y postoperatively and among each factor in the RSS with the risk of PKP graft failure using univariate and multivariate ***：The retrospective cohort study had 152 PKPs from 152 patients. Eighteen cases were excluded from our study due to primary failure（10 cases）,incomplete medical notes（5 cases）and follow-up less than 1y（3cases）. We included 134 PKPs from 134 patients stratified by preoperative risk score. Spearman coefficient was calculated for the relationship between the score obtained and risk of failure at 1y. Univariate and multivariate analysis were calculated for the impact of every single risk factor included in the RSS over graft failure at 1y. RESULTS：Spearman coefficient showed statistically significant correlation between the score in the RSS and graft failure（P〈0.05）. Multivariate logistic regression analysis showed no statistically significant relationship（P〉0.05）between diagnosis and lens status with graft failure. The relationship between the other risk factors studied and graft failure was significant（P〈0.05）,although the results for previous grafts and graft failure was unreliable. None of our patients had previous blood transfusion,thus,it had no impact. CONCLUSION：After the application of multivariate analysis techniques,some risk factors do not show the expected impact over graft failure at 1y.

关键词： penetrating keratoplasty graft failure score

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Molecular diagnosis of Chagas disease:a systematic review and meta-analysis

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Infectious Diseases of Poverty 2023年第5期12卷 93-93页

作者： guillermo Pascual-Vázquez Montserrat Alonso-Sardón Beatriz Rodríguez-Alonso Javier Pardo-Lledías Angela Romero Alegría Pedro Fernández-Soto Juan luis Muñoz Bellido Antonio Muro Moncef Belhassen-García Infectious and Tropical Diseases Group (E-INTRO) Biomedical Research Institute of Salamanca (IBSAL) Tropical Diseases Research Center of the University of Salamanca (CIETUS) Faculty of Pharmacy University of Salamanca Salamanca Spain Area of Preventive Medicine Epidemiology and Public Health Department of Biomedical and Diagnostic Sciences Faculty of Medicine E-INTRO IBSAL CIETUS University of Salamanca Salamanca Spain Internal Medicine Service University Health Care Complex of Salamanca (CAUSA) E-INTRO IBSAL CIETUS Salamanca Spain Internal Medicine Department Hospital Marqués de Valdecilla University of Cantabria IDIVAL (Valdecilla Research Institute) Santander Spain Internal Medicine Service CAUSA. E-INTRO IBSAL CIETUS Salamanca Spain Infectious and Tropical Diseases Group (E-INTRO) IBSAL. CIETUS. Faculty of Pharmacy University of Salamanca Salamanca Spain Microbiology and Parasitology Service CAUSA IBSAL CIETUS CSIC Department of Biomedical and Diagnostic Sciences University of Salamanca Salamanca Spain Internal Medicine Service. Infectious Diseases Section CAUSA IBSAL CIETUS University of Salamanca Salamanca Spain

Background The complexity of the Chagas disease and its phases is impossible to have a unique test for both phases and a lot of different epidemiological ***,serology is the reference standard technique;occasionally,results are inconclusive,and a different diagnostic technique is *** guidelines recommend molecular testing.A systematic review and meta-analysis of available molecular tools/techniques for the diagnosis of Chagas disease was performed to measure their heterogeneity and efficacy in detecting Trypanosoma cruzi infection in blood *** A systematic review was conducted up to July 27,2022,including studies published in international *** and exclusion criteria were defined to select eligible *** were extracted and presented according to PRISMA 2020 *** quality was assessed using Quality Assessment of Diagnostic Accuracy Studies-2(QUADAS-2).A random-effects model was used to calculate pooled sensitivity,specificity,and diagnostic odds ratio(DOR).Forest plots and a summary of the receiving operating characteristics(SROC)curves displayed the *** was determined by I^(2)and Tau^(2)statistics and P *** plots and Deek's test were used to assess publication bias.A quantitative meta-analysis of the different outcomes in the two different clinical phases was *** We identified 858 records and selected 32 *** pertained to endemic countries and nonendemic areas with adult and paediatric *** sample sizes ranged from 17 to 708 *** were no concerns regarding the risk of bias and applicability of all included studies.A positive and nonsignificant correlation coefficient(S=0.020;P=0.992)was obtained in the set of studies that evaluated diagnostic tests in the acute phase population(ACD).A positive and significant correlation coefficient(S=0.597;P<0.000)was obtained in the case of studies performed in the chronic phase population(

关键词： Chagas disease Trypanosoma cruzi Molecular diagnosis Polymerase chain reaction Loop-mediated isothermal amplification

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Characteristics of Thyroid Hormones in Hypertensive Hispanic Population

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Journal of Life Sciences 2012年第2期6卷 155-163页

作者： Rodrigo Verdugo Graciela Wendel Liliana Trujillo guillermo Orellano Lucia Fuentes Department of Pharmacology National University of San Luis San Luis 5700 Argentina D.O.S.P.U. Cardiologic Center San Luis 5700 Argentina Orellano-Elorza Biochemical Laboratory San Luis 5700 Argentina

The aim was to characterize thyroid function in Hispanic hypertensive population. Retrospective study of 1,023 individuals was performed, in which 68.23% had hypertension （74.21% women） and 37.25% had thyroid pathology. Age, weight, blood pressure, biochemical measurements were recorded, mean age： 53.02 ± 14.80 yr, mean weight： 83.05 ±16.95 kg, thyrotropin-stimulating hormone （TSH） hypertensive patients： 5.55±1.63 μUI/mL and without hypertension： 3,72 ± 1.60 μUI/mL. TSH range concentration distributed by sex indicates： 1.50-2.49 μUI/mL men, 2.50-5.01 μUI/mL women. TSH categories vs. age were positively associated （r = ＋ 0.114, P = 0.044） and hypertension by age was also correlated （r = ＋ 0.178, P = 0.0001）. Significant positive association we found between TSH and diastolic blood pressure （r = ＋ 0.197, P = 0.008）. Systolic and diastolic blood pressure vs. age were positively increasing （r = ＋ 0.410,p = 0.0001; r = ＋0.285, P = 0.0001, respectively）. Prevalence of thyroid pathology in hypertensive subjects are hyperthyroidism 5.87%; hypothyroidism 20.34% （5.73% subclinic） and autoimmune disease 6.25%. High percentage of hypertensive population has concomitant thyroid diseases, more common in women （50-70 years）. Systematic surveillance for occult thyroid dysfunction in patients with hypertension could prevent future cardiovascular disease.

关键词： Thyroid hormones blood pressure thyroid pathologies patients Hispanic.

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Effect of Time and Fatty Acid Composition in Eggs of White Leghorn Hens Supplemented with Tuna Oil

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Food and Nutrition Sciences 2013年第9期4卷 39-44页

作者： Jesus Eduardo Morales-Barrera Mariano Jesus Gonzalez-Alcorta Rosa Maria Castillo-Dominguez Omar Francisco Prado-Rebolledo Jose luis Vazquez Xochitl Hernandez-Velasco guillermo Tellez Anita Menconi Billy Marshal Hargis Silvia Carrillo-Dominguez Departamento de Producción Agrícola y Animal Universidad Autónoma Metropolitana México City México Department of Poultry Science University of Arkansas Fayetteville USA. Depto. Nutrición Animal Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán México City México Facultad de Medicina Veterinaria y Zootecnia Universidad de Colima Colima México Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autónoma de México México City México Universidad Autónoma Chapingo Estado de México México

The study evaluated the effect of time and fatty acid (FA) composition in eggs of laying hens supplemented with tuna oil (TO). Two hundred White Leghorn hens 30-week were divided into 5 treatments with 4 replicates of 10 birds each. Treatments were assigned randomly and consisted of 0%, 0.75%, 1.50%, 2.25% and 3% TO in commercial sorghum-soybean meal diets, in which the soybean oil was partially replaced. The experiment was conducted for 90 days. At days 28, 56 and 84 of the experiment, 10 eggs per replicate were collected for chemical analysis. Feed intake, egg production rate, egg weight, and feed conversion were not influenced by dietary treatment. FA content was significantly altered (P < 0.05) by TO, showing a progressive increase in egg n-3 FA (especially docosahexaenoic [DHA] and eicosapentaenoic [EPA] acids) when TO was added. Levels of EPA and DHA were higher (P < 0.05) in the egg lipids of TO fed hens than those in the control group. This was correlated (P < 0.05) with the concentration of TO in the diet. However, no significant differences were observed either in egg weight or percent of egg lay between groups. There was a high correlation (P < 0.05) between TO inclusion level in the diet and the incorporation of EPA (r = 0.96) or DHA (r = 0.92) into the egg yolk and an overall decrease in n-6 FA. There was a high correlation (P < 0.05) between TO inclusion level and the incorporation of linoleic acid (LA, r = -0.95) or arachidonic acid (AA, r = -0.96) into the egg yolk. The highest incorporation (P < 0.05) of total n-3 FA content in eggs was obtained with 3% TO/kg. This increase was proportional to TO inclusion levels in the diets (r = 0.95). The results indicate that the n-3 FA content in eggs can be increased by dietary supplementation with TO, and that TO can serve as a reasonable alternative feed ingredient in layer diets to produce a healthier choice of egg.

关键词： Egg Enrichment Laying Hens n-3 Fatty Acids Tuna Oil

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Current trends of liver cirrhosis in Mexico: Similitudes and differences with other world regions

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World Journal of Clinical Cases 2018年第15期6卷 922-930页

作者： Nahum Méndez-Sánchez Felipe Zamarripa-Dorsey Arturo Panduro Emma Purón-González Edgar Ulises Coronado-Alejandro Carlos Alejandro Cortez-Hernández Fátima Higuera de la Tijera José luis Pérez-Hernández Eira Cerda-Reyes Heriberto Rodríguez-Hernández Vania César Cruz-Ramón Oscar Lenin Ramírez-Pérez Nancy Edith Aguilar-Olivos Olga Fabiola Rodríguez-Martínez Samantha Cabrera-Palma guillermo Cabrera-álvarez Liver Research Unit Medica Sur Clinic and Foundation Department of Gastroenterology Hospital "Juárez" of México Department of Molecular Biology in Me-dicine Civil Hospital of Guadalajara "Fray Antonio Alcalde" Department of Internal Medicine "Christus Muguerza "Super Specialty" Hospital" Monterrey Department of Gas-troenterology "University Hospital "Jose Eleuterio González" Monterrey Department of Gastroenterology "General Hospital of Mexico "Dr.Eduardo Liceaga" Department of Gastroenterology "Central Military Hospital" Faculty of Medicine and Nutrition Juárez University of the State of Durango Liver Clinic Regional General Hospital IMSS 1

AIM To investigate the main current etiologies of cirrhosis in *** We performed a cross-sectional retrospective multicenter study that included eight hospitals in different areas of Mexico. These hospitals provide health care to people of diverse social classes. The inclusion criteria were a histological, clinical, biochemical, endoscopic, or imaging diagnosis of liver cirrhosis. Data were obtained during a 5-year period(January 2012-December 2017). RESULTS A total of 1210 patients were included. The mean age was 62.5 years(SD = 12.1), and the percentages of men and women were similar(52.0% vs 48.0%). The most frequent causes of liver cirrhosis were hepatitis C virus(HCV)(36.2%), alcoholic liver disease(ALD)(31.2%), and nonalcoholic steatohepatitis(23.2%), and the least frequent were hepatitis B virus(1.1%), autoimmune disorders(7.3%), and other conditions(1.0%).CONCLUSION HCV and ALD are the most frequent causes of cirrhosis in Mexico. However, we note that non-alcoholic fatty liver disease(NAFLD) as an etiology of cirrhosis increased by 100% compared with the rate noted previously. We conclude that NAFLD will soon become one of the most frequent etiologies of liver cirrhosis in Mexico.

关键词： Alcoholic liver disease Hepatitis C virus Nonalcoholic steatohepatitis Liver cirrhosis

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Diabetic gastroenteropathy:An underdiagnosed complication

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World Journal of Diabetes 2021年第6期12卷 794-809页

作者： Marcio JoséConcepción Zavaleta Jhean Gabriel Gonzáles Yovera Diego Martín Moreno Marreros Luciana del Pilar Rafael Robles Kely Roxana Palomino Taype Karen Nohelly Soto Gálvez luis Fernando Arriola Torres Julia Cristina Coronado Arroyo luis Alberto Concepción Urteaga Division of Endocrinology Hospital Nacional Guillermo Almenara IrigoyenLima 12590Peru Division of Gastroenterology Hospital Nacional Guillermo Almenara IrigoyenLima 12590Peru Division of Medicine Universidad Nacional de TrujilloTrujillo 13011Peru Division of Endocrinology Hospital Nacional Dos de MayoLima 150101Peru Division of Endocrinology Instituto de Neurología y Endocrinología AvanzadaAndahuaylas 03700Peru Clinical Management Unit Hospital Nacional Guillermo Almenara IrigoyenLima 12590Peru Division of Neurology Instituto de Neurología y Endocrinología AvanzadaAndahuaylas 03700Peru Division of Obstetrics and Gynaecology Clínica VesalioLima 15036Peru Division of Neumology Hospital Regional Docente de TrujilloTrujillo 13011Peru

This article is an extensive review that provides an update on the pathophysiology,symptoms,diagnosis,and treatment of diabetic *** is no reported prevalence,but it has been described that patients with type 1 diabetes have a cumulative incidence at 10 years of 5.2%,and type 2 patients,1%.Also,in the group of type 1 diabetes,it has been observed that women are more likely to present this condition(5.8%vs 3.5%).Many factors are associate with its development(e.g.,hyperglycemia,vagal dysfunction,loss of expression of neural nitric oxide synthase in the myenteric plexus,alterations in the Cajal interstitial cell network,and oxidative stress).Gastrointestinal discomfort could be perceived 70% higher in diabetic patients,describing that 25%of diabetic patients experience gastrointestinal *** enteropathy could affect any portion of the gastrointestinal tract,but esophageal alterations were described in more than 60% of diabetic patients,also 60% of them present constipation,and 20%,*** emptying scintigraphy is useful to evaluate gastroparesis,therefore,gastric retention of more than 60%at 2 h has a sensitivity of 100% and specificity of 20% for diagnosis;however,other studies such as breath tests,with a sensitivity of 89% and a specificity of 80%,or the endoscopic capsule contribute to the *** is no cure;however,management must be multidisciplinary,focused on slowing the progression of diabetic gastroenteropathy,reducing symptoms,and restoring function;that includes nutritional recommendation,maintain glucose levels kept below 180 mg/dL,use of prokinetics,anti-emetics;nowadays,it has been special interest in surgical treatment,such as pyloroplasty,also gastric electrical stimulation appears to be another alternative.

关键词： Gastroenteropathy Gastroparesis Diabetes mellitus Complication Gastrointestinal disease Treatment

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Arylated analogues of cypronazole: fungicidal effect and activity on human fibroblasts. Docking analysis and molecular dynamics simulations

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Natural Products and Bioprospecting 2022年第1期12卷 107-120页

作者： Natividad Herrera Cano Sebastian A.Andujar Cristina Theoduloz Daniel A.Wunderlin Ana N.Santiago guillermo Schmeda-Hirschmann Ricardo D.Enriz Gabriela E.Feresin Instituto de Biotecnología Facultad de IngenieríaUniversidad Nacional de San JuanCONICET-CCT San JuanAv.Libertador General San Martín1109(O)5400 San JuanArgentina Facultad de Química Bioquímica y Farmacia-IMIBIO-SL(CONICET)Universidad Nacional de San LuisChacabuco 9155700 San LuisArgentina Laboratorio de Cultivo Celular Facultad de Ciencias de la SaludUniversidad de TalcaCasilla 7473460000 TalcaChile ICYTAC CONICET and Universidad Nacional de CórdobaFacultad de Ciencias QuímicasDepartamentoQuímica OrgánicaCiudad UniversitariaBv.Juan Filloy s/n5000 CórdobaArgentina INFIQC CONICET and Universidad Nacional de CórdobaFacultad de Ciencias QuímicasDepartamento Química OrgánicaCiudad UniversitariaHaya de La Torre S/N5000 CórdobaArgentina Laboratorio de Química de Productos Naturales Instituto de Química de Recursos NaturalesUniversidad de TalcaCasilla 7473460000 TalcaChile

Triadimefon(TDM)and cyproconazole(CPZ)are two triazoles widely used as *** azoles were synthe-sised starting from commercial TDM and *** compounds were evaluated against phytopathogenic filamentous fungi,including Aspergillus fumigatus(AF),***(AN),***(AU),***(AJ),***(AT),Fusarium oxyspo-rum and Botrytis cinerea isolated from grapevine in the province of San Juan,*** of the synthesised compounds(1-(Biphenyl-4-yloxy)-3,3-dimethyl-1-(1H-1,2,4-triazol-1-yl)butan-2-one,1;2-(Biphenyl-4-yl)-3-cyclopropyl-1-(1H-1,2,4-triazol-1-yl)butan-2-ol,3;3-Cyclopropyl-2-(4’-fluorobiphenyl-4-yl)-1-(1H-1,2,4-triazol1-yl)butan-2-ol,4)presented remarkable in vitro fungicidal properties,with better effects than TDM and CPZ on some of the target *** was assessed using human lung fibroblasts *** 1,with IC50 values of 389.4μM,was less toxic towards MRC-5 human lung fibroblasts than commercial TDM(248.5μM)and CPZ(267.4μM).Docking analysis and molecular dynamics simulations suggest that the compounds present the same interaction in the binding pocket of the CYP51B enzyme and with the same amino acids as *** derivatives investigated could be considered broad-spectrum but with some selectivity towards imperfect fungi.

关键词： Azoles Antifungal cytotoxicity Conformational and electronic analysis

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The domestication of Cucurbita argyrosperma as revealed by the genome of its wild relative

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Horticulture Research 2021年第1期8卷 1287-1300页

作者： Josue Barrera-Redondo guillermo Sanchez-de la Vega Jonas A.Aguirre-Liguori Gabriela Castellanos-Morales Yocelyn T.Gutierrez-Guerrero Xitlali Aguirre-Dugua Erika Aguirre-Planter Maud I.Tenaillon Rafael Lira-Saade luis E.Eguiarte Departamento de Ecologia Evolutiva Instituto de EcologiaUniversidad Nacional Autonoma de MexicoCircuito Exterior s/n Anexo al Jardin Botanico04510 Ciudad de MexicoMexico Department of Ecology and Evolutionary Biology University of CaliforniaIrvineCA 92697USA Departamento de Conservacion de la Biodiversidad El Colegio de la Frontera SurVillahermosaCarretera Villahermosa‐Reforma km 15.5 Rancheria El Guineo 2ªseccion86280 VillahermosaTabascoMexico Genetique Quantitative et Evolution-Le Moulon Universite Paris-SaclayInstitut National de Recherche pour l’Agriculturel’Alimentation et l’EnvironnementCentre National de la Recherche ScientifiqueAgroParisTechGif-sur-Yvette 91190France UBIPRO Facultad de Estudios Superiores IztacalaUniversidad Nacional Autonoma de MexicoAv.de los Barrios#1Col.Los Reyes IztacalaTlalnepantlaEdo.de Mex 54090Mexico

Despite their economic importance and well-characterized domestication syndrome,the genomic impact of domestication and the identification of variants underlying the domestication traits in Cucurbita species(pumpkins and squashes)is currently *** argyrosperma,also known as cushaw pumpkin or silver-seed gourd,is a Mexican crop consumed primarily for its seeds rather than fruit *** makes it a good model to study Cucurbita domestication,as seeds were an essential component of early Mesoamerican diet and likely the first targets of human-guided selection in pumpkins and *** obtained population-level data using tunable Genotype by Sequencing libraries for 192 individuals of the wild and domesticated subspecies of *** across *** also assembled the first high-quality wild Cucurbita *** genomic analyses revealed several structural variants and presence/absence of genes related to *** results indicate a monophyletic origin of this domesticated crop in the lowlands of *** found evidence of gene flow between the domesticated and wild subspecies,which likely alleviated the effects of the domestication *** uncovered candidate domestication genes that are involved in the regulation of growth hormones,plant defense mechanisms,seed development,and *** presence of shared selected alleles with the closely related species Cucurbita moschata suggests domestication-related introgression between both taxa.

关键词： Cucurbita likely pump

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Mapping aboveground biomass by integrating geospatial and forest inventory data through a k-nearest neighbor strategy in North Central Mexico

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Journal of Arid Land 2014年第1期6卷 80-96页

作者： Carlos A AGUIRRE-SALADO Eduardo J TREVIO-GARZA Oscar A AGUIRRE-CALDERóN Javier JIMNEZ-PREZ Marco A GONZLEZ-TAGLE José R VALDZ-LAZALDE guillermo SNCHEZ-DíAZ Reija HAAPANEN Alejandro I AGUIRRE-SALADO Liliana MIRANDA-ARAGóN Faculty of Forest Sciences Autonomous University of Nuevo Leon Faculty of Engineering Autonomous University of San Luis Potosi Forestry Program Postgraduate College Haapanen Forest Consulting Faculty of Agronomy and Veterinary Autonomous University of San Luis Potosí

As climate change negotiations progress,monitoring biomass and carbon stocks is becoming an important part of the current forest ***,national governments are interested in developing forest-monitoring strategies using geospatial *** statistical methods for mapping biomass,there is a nonparametric approach called k-nearest neighbor(kNN).We compared four variations of distance metrics of the kNN for the spatially-explicit estimation of aboveground biomass in a portion of the Mexican north border of the intertropical *** derived,climatic,and topographic predictor variables were combined with the Mexican National Forest Inventory(NFI)data to accomplish the *** of distance metrics applied into the kNN algorithm was evaluated using a cross validation leave-one-out *** results indicate that the Most Similar Neighbor(MSN)approach maximizes the correlation between predictor and response variables(r=0.9).Our results are in agreement with those reported in the *** findings confirm the predictive potential of the MSN approach for mapping forest variables at pixel level under the policy of Reducing Emission from Deforestation and Forest Degradation(REDD+).

关键词： k-nearest neighbor Mahalanobis most similar neighbor MODIS BRDF-adjusted reflectance forest inventory the policy of Reducing Emission from Deforestation and Forest Degradation

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The Bovine Tuberculosis Campaign in Mexico： Present and Perspectives

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Journal of Agricultural Science and Technology(A) 2014年第4期4卷 277-290页

作者： Jose Alfredo Gutierrez-Reyes Estela Flores-Velazquez guillermo Agustin Reyes-Escalona Joaquin BraulioDelgadillo-Alvarez Marcela Amalia Mercado-Pezzat Eli Tonatiuh Selva-Hernandez Alejandro Perera-Ortiz Jose Alberto Ordufia-Sumaran Cecilia Romero-Torres luis Antonio Jimenez-Zamudio Ethel AwildaGarcfa-Latorre2 and Citlatepetl Salinas-Lara General Directorate of Animal Health National Service of Health Food Safety and Food Quality (SENASICA) Mexico City 03310 Mexico National School of Biological Sciences National Polythecnical Institute Santo Tomas Unit Lazaro Cardenas Mexico City 11340 Mexico

Mexico is considered by different international organizations as a country where the bovine tuberculosis is an endemic problem. However, there are important and consistent improvements in eradicating the disease by the implementation of government policies and with the livestock organization support. The current situation of the program shows good results regarding decreasing the prevalence, and as a consequence, the risk of this disease for public health and animal health, as well as the improvement in animal production and international and national trade. Today, the bovine tuberculosis distribution is almost known in Mexico. In addition, a series of controls and indicators have been established, which allow maintain consistency and reliability in the data that are managed and analyzed.

关键词： Bovine tuberculosis surveillance status quarantine Mexico.

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