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Detection of Distorted Segregation in genotype of Pollen Calli Derived from Hybrid F_1 of Cultivated Rice (Oryza sativa L.) Using SSR Markers

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Agricultural Sciences in China 2006年第6期5卷 412-416页

作者： YAO Yan lu yong-gen LIU Xiang-dong FENG Jiu-huan ZHANG Gui-quan Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 P.R.China

S-a, S-b and S-c are three loci for F1 pollen sterility in cultivated rice （Oryza sativa L.）. Taichung 65 （T65） is all Sj/Sj at these three loci, while its F1 pollen sterile near-isogenic lines, TISL2 （S-b）, TISL4 （S-a） and TISL5 （S-c） is Sj/Sj according to their respective sterility locus. Using SSR molecular marker to detect the segregation of the allele Si and Sj in pollen calli population induced from different hybrid F1, which have different pollen sterility locus, showed that the segregation of allele Si and Sj was distorted. The distorted direction of pollen calli population in vitro was not the same as F2 population in vivo. The quantities of pollen callus carrying Sj were much more than that of carrying Siat S-a and S-c locus, the ratio of Si and Sj were 1：4.81 and 1：1.96 respectively. But the opposite tendency was observed at S-b locus, the ratio of Si and Sj being 1：0.35. At the same time, all these results were undisturbed by either culture medium or culture period.

关键词： rice (Oryza sativa L.) pollen sterility pollen callus distorted segregation SSR marker

Progress on Transferring Elite genes from Non-AA genome Wild Rice into Oryza sativa through Interspecific Hybridization

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Rice science 2008年第2期15卷 79-87页

作者： Fu Xue-lin lu yong-gen LIU Xiang-dong LI Jin-quan Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China

The progress of research on transferring elite genes from non-AA genome wild rice into Oryza sativa through interspecific hybridization are in three respects, that is, breeding monosomic alien addition lines （MAALs）, constructing introgression lines （ILs） and analyzing the heredity of the characters and mapping the related genes. There are serious reproductive barriers, mainly incrossability and hybrid sterility, in the interspecific hybridization of O. sativa with non-AA genome wild rice. These are the ＇bottleneck＇ for transferring elite genes from wild rice to O. sativa. Combining traditional crossing method with biotechnique is a reliable way to overcome the reproductive barriers and to improve the utilizing efficiency of non-AA genome wild rice.

关键词： Oryza sativa non-AA genome wild rice interspecific hybridization monosomic alien addition line introgression line reproductive barrier gene mapping

Comparative Embryological Studies on Infertility of Interspecific Hybridizations Between Oryza sativa with Different Ploidy Levels and O. officinalis

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Rice science 2009年第1期16卷 58-64页

作者： FU Xue-lin lu yong-gen LIU Xiang-dong LI Jin-quan ZHAO Xing-juan Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China

As maternal parents, diploid （L202-2x） and autotetraploid （L202-4x） of Oryza sativa cv. L2O2 were crossed with O. officinalis. Embryo development and fertilization in these two crosses were comparatively studied. There were no mature hybrid seeds obtained because all the hybridized spikelets died 30 days after pollination. The main reasons for no seed set were abnormal fertilization and development of the embryos and endosperms in the interspecific hybrids. There were doublefertilization, egg cell single-fertilization and non-fertilization in these crosses. Although 59.45% and 54.87% of hybrid embryos produced in the crosses of L202-2x/O. officinalis and L202-4x/O. officinalis, respectively, hybrid embryos ceased to develop or degenerated and plenty of free endosperm nuclei were in disaggregating state without developing cellular endosperms three days after pollination. Besides, some embryological differences in these two crosses were found, that is, the rate of double-fertilization and total rate of doubleand single-fertilization in L202-2x/O. officinalis were higher than those in L202-4x/O. officinalis. The embryo and endosperm of hybrids developed more slowly, and embryos and free endosperm nuclei were more severely degenerated in L202-4x/O. officinalis than in L202-2x/O. officinalis. Five days after pollination, a few of embryos in L202-2x/O. officinalis developed into pear-shaped ones, however, embryos in L202-4x/O. officinalis were all degenerated. Therefore, it is more difficult to obtain interspecific hybrids by wide crosses between autotetraploid of O. sativa and O. officinalis.

关键词： Oryza sativa Oryza officinalis diploid rice autotetraploid rice wild rice interspecific hybridization embryo development infertility

In-situ thermal Raman mapping and stress analysis of CNT/CF/epoxy interfaces

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新型炭材料（中英文） 2024年第4期39卷 703-714页

作者： HE Jing-zong CHEN Shi MA Zheng-kun lu yong-gen WU Qi-lin State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China

A study of the interfacial behavior and internal thermal stress distribution in fiber-reinforced composites is essential to assess their performance and ***/carbon fiber(CF)hybrid fibers were constructed using electrophoretic *** interfacial properties of CF/epoxy and CNT/CF/epoxy composites were statistically investigated and compared using in-situ thermal Raman mapping by dispersing CNTs as a Raman sensing medium(CNT_(R))in a *** associated local thermal stress changes can be simulated by capturing the G'band position distribution of CNT_(R) in the epoxy at different *** was found that the G'band shifted to lower positions with increasing temperature,reaching a maximum difference of 2.43 cm^(−1) at 100℃.The interfacial bonding between CNT/CF and the matrix and the stress distribution and changes during heat treatment(20-100℃)were investig-ated in *** work is important for studying thermal stress in fiber-reinforced composites by in-situ thermal Raman mapping technology.

关键词： Thermal Raman mapping Stress distribution Carbon fiber Carbon nanotube Interface

Fluorescence color tuning of dual-emission carbon quantum dots pro-duced from biomass and their use in Fe3+and Cu2+detection

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新型炭材料（中英文） 2024年第6期39卷 1213-1226页

作者： XUE Jia-jia GAN Mei-heng lu yong-gen WU Qi-lin State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China

Using simple and eco-friendly ethanol solvothermal treatment,dual-emission biomass carbon quantum dots(D-BCQDs)were synthesized from biomass viburnum awabuki *** excitation with 413 nm wavelength light two emission peaks appeared at 490 and 675 nm and the dots could be tuned to emit crimson,red,purplish red,purple and blue-gray fluorescence by changing the solvothermal temperature from 140℃ to 160,180,200 and 240℃,*** and FTIR characterization in-dicated that the fluorescence color was mainly determined by surface oxidation defects,elemental nitrogen and sp^(2)-C/sp^(3)-C hybrid-ized structural *** D-BCQDs could not only detect Fe^(3+)or Cu^(2+),but also quantify the concentration ratio of Fe^(3+)to Cu^(2+)in a solution containing both,demonstrating their potential applications in the simultaneous detection of Fe^(3+)and Cu^(2+)ions.

关键词： Dual-emission biomass carbon quantum dots Multicolor Solvothermal method Ion detection

Abnormal Structure of Embryo Sac in Autotetraploid Rice

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Rice science 2006年第4期13卷 257-264页

作者： Guo Hai-bin FENG Jiu-huan lu yong-gen LIU Xiang-dong Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China

The structures of mature embryo sacs in 13 genetic stock lines of autotetraploid rice （Oryza sativa L.）, including indica, japonica and javanica, were studied by using the whole-mount stain-clearing laser scanning confocal microscopy （WCLSM）. Among the 13 autotetraploid rice, the majority of ovaries possess normal polygonum-type embryo sacs, while a few ovaries were characterized by abnormal embryo sacs. The abnormalities of embryo sacs could be classified into six categories, i. e. no female germ unit, abnormal polar nuclei, embryo sac degeneration, no egg apparatus, small embryo sac and ＇double set＇ of embryo sacs. The frequency of abnormal embryo sac in japonica （26.6%） was higher than that in indica （19.34%）. In addition, the major abnormalities in each autotetraploid line varied, suggesting that the abnormalities may be related to the genotypes of the varieties.

关键词： autotetraploid rice structure anatomy embryo sac seed setting rate

聚丙烯腈凝胶纤维渗硼对炭纤维的石墨化过程的影响

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新型炭材料 2019年第1期34卷 95-104,127页

作者：陈力吕春祥蒋俊祺吕永根东华大学材料科学与工程学院碳纤维材料改性国家重点实验室上海201620 中国科学院山西煤炭化学研究所碳纤维制备技术国家工程实验室山西太原030001

对聚丙烯腈凝胶纤维进行渗硼处理所制炭纤维在1600～2400℃进行石墨化热处理,利用电感耦合等离子体光谱仪、扫描电子显微镜、能谱仪、X射线光电子能谱仪、X射线衍射仪和万能力学测试机对石墨纤维进行表征,研究了硼对其结构及性能的影响... 详细信息

对聚丙烯腈凝胶纤维进行渗硼处理所制炭纤维在1600～2400℃进行石墨化热处理,利用电感耦合等离子体光谱仪、扫描电子显微镜、能谱仪、X射线光电子能谱仪、X射线衍射仪和万能力学测试机对石墨纤维进行表征,研究了硼对其结构及性能的影响。结果表明,在石墨化温度范围内,随着温度的升高,炭纤维中的硼含量逐渐降低。硼在石墨化过程中以取代的方式进入到碳网内并在其中进行扩散,但因炭纤维中硼含量较低,其并未对微晶尺寸的生长产生影响,而明显提高了取向度,导致炭纤维的杨氏模量提高的同时,其拉升强度的降幅也显著降低。在达到相同模量的情况下,含硼炭纤维的石墨化温度可降低200℃。

关键词：聚丙烯腈凝胶纤维硼催化石墨化微晶结构力学性能

Strategies on Sample Size Determination and Qualitative and Quantitative Traits Integration to Construct Core Collection of Rice (Oryza sativa)

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Rice science 2011年第1期18卷 46-55页

作者： LI Xiao-ling lu yong-gen LI Jin-quan XU Hai-ming Muhammad Qasim SHAHID Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China Department of Agronomy College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China Now work at Hubei Provincial Key Laboratory of Natural Products Research and Development China Three Gorges University Yicang 443002 China

The development of a core collection could enhance the utilization of germplasm collections in crop improvement programs and simplify their management. Selection of an appropriate sampling strategy is an important prerequisite to construct a core collection with appropriate size in order to adequately represent the genetic spectrum and maximally capture the genetic diversity in available crop collections. The present study was initiated to construct nested core collections to determine the appropriate sample size to represent the genetic diversity of rice landrace collection based on 15 quantitative traits and 34 qualitative traits of 2 262 rice accessions. The results showed that 50-225 nested core collections, whose sampling rate was 2.2%-9.9%, were sufficient to maintain the maximum genetic diversity of the initial collections. Of these, 150 accessions （6.6%） could capture the maximal genetic diversity of the initial collection. Three data types, i.e. qualitative traits （QT1）, quantitative traits （QT2） and integrated qualitative and quantitative traits （QTT）, were compared for their efficiency in constructing core collections based on the weighted pair-group average method combined with stepwise clustering and preferred sampling on adjusted Euclidean distances. Every combining scheme constructed eight rice core collections （225, 200, 175, 150, 125, 100, 75 and 50）. The results showed that the QTT data was the best in constructing a core collection as indicated by the genetic diversity of core collections. A core collection constructed only on the information of QT1 could not represent the initial collection effectively. QTT should be used together to construct a productive core collection.

关键词： nested core collection sample size quantitative traits qualitative traits integrated qualitative and quantitative traits rice

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中间相沥青的聚合动力学及组分控制

新型炭材料 2020年第5期35卷 591-598页

作者：梁正午吕永根孙祝林罗含东华大学纤维材料改性国家重点实验室材料科学与工程学院上海201620

增加中间相沥青中吡啶可溶物含量对于提高其可纺性具有重要意义。本研究以萘系各向同性沥青为原料,使用直接热缩聚合成中间相沥青,分析了聚合产物族组成随时间的变化;结合偏光显微镜和元素分析结果,研究了聚合温度、时间和原料组成对中... 详细信息

增加中间相沥青中吡啶可溶物含量对于提高其可纺性具有重要意义。本研究以萘系各向同性沥青为原料,使用直接热缩聚合成中间相沥青,分析了聚合产物族组成随时间的变化;结合偏光显微镜和元素分析结果,研究了聚合温度、时间和原料组成对中间相沥青合成反应的影响;并用连串反应模型计算了反应动力学常数。结果表明,400℃预聚10 h后,430℃下反应20 h得到族组成分布相对均一的沥青。中间相沥青合成反应具有很明显的加速效应,即重组分的反应速度常数较轻组分大。在合成反应后期,随着沥青分子量增加,其反应速率也越来越快。因此起始组成、反应温度与时间对族组成的分布有决定性的影响。

关键词：中间相沥青热缩聚反应动力学