检索结果-南通市图书馆

Epigenetic changes of pituitary tumor-derived transforming gene 1 in pancreatic cancer

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2008年第3期7卷 313-317页

作者： Zhang, Mang-Li lu, sen Zheng, Shu-sen Zhejiang Univ Sch Med Affiliated Hosp 1 Dept Hepatobiliary & Pancreat Surg Hangzhou 310003 Zhejiang Peoples R China

BACKGROUND: Pancreatic cancer is a devastating disease with abnormal genetic changes. The pituitary tumor-derived transforming gene (PTTG) is considered to be implicated in the tumorigenesis of cancers when the gene is epigenetically transformed. In this study, we investigated the relationships between aberrant expression and epigenetic changes of the PTTG1 gene in pancreatic cancer. METHODS: We chose 4 cell lines (PANC-1, Colo357, T3M-4 and PancTu I) and pancreatic ductal adenocarcinoma (PDAC) tissues. After using restriction isoschizomer endonucleases (Msp I /Hpa II) to digest the DNA sequence (5'-CCGG-3'), we performed PCR reaction to amplify the product. And RT-PCR was applied to determine the gene expression. RESULTS: The mRNA expression of the PTTG1 gene was higher in pancreatic tumor than in normal tissue. The gene was also expressed in the 4 PDAC cell lines. The methylation states of the upstream regions of the PTTG1 gene were almost identical in normal, tumor pancreatic tissues and the 4 PDAC cell lines. Some (5'-CCGG-3') areas in the upstream region of PTTG1 were methylated, while some others were unmethylated. CONCluSIONS: The oncogene PTTG1 was overexpressed in pancreatic tumor tissues and verified by RT-PCR detection. The methylation status of DNA in promoter areas was involved in the gene expression with the help of other factors in pancreatic cancer.

关键词： pancreatic neoplasms pituitary tumor-derived transforming gene epigenesis, genetic

Correlation between ECT2 gene expression and methylation change of ECT2 promoter region in pancreatic cancer

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2008年第5期7卷 533-538页

作者： Zhang, Mang-Li lu, sen Zhou, Lin Zheng, Shu-sen Zhejiang Univ Sch Med Affiliated Hosp 1 Dept Hepatobiliary & Pancreat Surg Hangzhou 310003 Zhejiang Peoples R China

BACKGROUND: Pancreatic cancer is closely related to epigenetic abnormality. The epithelial cell transforming sequence 2 gene (ECT2) plays a critical role in Rho activation during cytokinesis, and thus may play a role in the pathogenesis of pancreatic cancer. In this study, we investigated the relationships between aberrant expression and epigenetic changes of the ECT2 gene in pancreatic cancer. METHODS: Four cell lines (PANC-1, Colo357, T3M-4 and PancTu I) and pancreatic ductal adenocarcinoma (PDAC) tissues were used for mRNA detection. After restriction isoschizomer endonucleases (Msp I/Hpa II) were used to digest the DNA sequence (5'-CCGG-3'), PCR was made to amplify the product. And RT-PCR was applied to determine the expression of the gene. RESULTS: The mRNA expression of the ECT2 gene was higher in pancreatic tumor tissue than in normal tissue. The gene was also expressed in the 4 PDAC cell lines. The methylation states of the upstream regions of the ECT2 gene were almost identical in normal, tumor pancreatic tissues, and the 4 PDAC cell lines. Some of the 5'-CCGG-3' areas in the upstream region of ECT2 were methylated, while others were unmethylated. CONCluSIONS: The oncogene ECT2 is overexpressed in pancreatic tumor tissues as verified by RT-PCR detection. The methylation status of DNA in promoter areas is involved in the gene expression, along with other factors, in pancreatic cancer.

关键词： pancreatic neoplasms epithelial cell transforming sequence 2 gene epigenesis, genetic

Mineralogical characterization of airborne individual particulates in Beijing PM10

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2006年第1期18卷 90-95页

作者： lu sen-lin SHAO Long-yi WU Ming-hong JIAO Zheng Shanghai Applied Radiation Institute School of Environmental and Chemical Engineering Shanghai University Shanghai 201800 China Department of Earth Science China University of Mining and Technology Beijing 100083 China

This work mainly focuses on the mineralogical study of particulate matter（PM10） in Beijing. Samples were collected on polycarbonate filter from April, 2002 to March, 2003 in Beijing urban area. Scanning electronic microscopy coupled with energy dispersive X-ray（SEM/EDX） was used to investigate individual mineral particles in Beijing PM10. 1454 individual mineral particulates from 48 samples were analysed by SEM/EDX. The results revealed that mineral particulates were complex and heterogeneous. 38 kinds of minerals in PM10 were identified. The clay minerals, of annual average percentage of 30.1%, were the main composition among the identified minerals, and illite/smectite was the main composition in clay minerals, reaching up to 35%. Annual average percentage of quartz, calcite, compound particulates, carbonates were 13.5%, 10.9%, 11.95%, 10.31%, respectively. Annual average percentage less than 10% were gypsum, feldspar, dolomite, and so on. Fluorite, apatite, halite, barite and chloridize zinc（ZnCl2） were firstly identified in Beijing PM10. Sulfurization was found on surface of mineral particles, suggested extensive atmospheric reaction in air during summer.

关键词： inhalable particulate matter(PM10) individual analysis mineral composition

Afforestation with an age-sequence of Mongolian pine plantation promotes soil microbial residue accumulation in the Horqin Sandy Land, China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Arid Land 2024年第4期16卷 567-579页

作者： GUO Jingwen SONG Xueshu WANG Xiao DU Zhangliu lu sen State Key Laboratory of Efficient Production of Forest Resources Research Institute of ForestryChinese Academy of ForestryBeijing 100091China Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjing 210037China College of Resources and Environmental Sciences China Agricultural UniversityBeijing 100193China

Land use change affects the balance of organic carbon(C)reserves and the global C *** residues are essential constituents of stable soil organic C(SOC).However,it remains unclear how microbial residue changes over time following *** this study,16-,23-,52-,and 62-year-old Mongolian pine stands and 16-year-old cropland were studied in the Horqin Sandy Land,*** analyzed changes in SOC,amino sugar content,and microbial parameters to assess how microbial communities influence soil C transformation and *** results showed that SOC storage increased with stand age in the early stage of afforestation but remained unchanged at about 1.27-1.29 kg/m2 after 52 ***,there were consistent increases in amino sugars and microbial residues with increasing stand *** stand age increased from 16 to 62 a,soil pH decreased from 6.84 to 5.71,and the concentration of total amino sugars increased from 178.53 to 509.99 mg/kg.A significant negative correlation between soil pH and the concentration of specific and total amino sugars was observed,indicating that the effects of soil acidification promote amino sugar stabilization during *** contrast to the Mongolian pine plantation of the same age,the cropland accumulated more SOC and microbial residues because of fertilizer *** Mongolian pine plantation with different ages,there was no significant change in calculated contribution of bacterial or fungal residues to SOC,suggesting that fungi were consistently the dominant contributors to SOC with increasing *** results indicate that afforestation in the Horqin Sandy Land promotes efficient microbial growth and residue accumulation in SOC stocks and has a consistent positive impact on SOC persistence.

关键词： soil organic matter stand age biomarker amino sugars microbial residues

Future therapeutic implications of new molecular mechanism of colorectal cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2023年第16期29卷 2359-2368页

作者： sen lu Cheng-You Jia Jian-She Yang Department of Surgical Anesthesiology First Affiliated Hospital of Anhui University of Science and TechnologyHuainan 232001Anhui ProvinceChina Department of Nuclear Medicine and Oncology Research Shanghai Tenth People’s HospitalTongji University School of MedicineShanghai 200072China

High incidence(10.2%)and mortality(9.2%)rates led to the ranking of colorectal cancer(CRC)as the second most malignant tumor spectrum worldwide in *** strategies are becoming highly dependent on the molecular characteristics of *** classical theories accept two models depicting the origin of CRC:The progression of adenoma to cancer and transformation from serrated polyps to ***,the molecular mechanism of CRC development is very *** instance,CRCs originating from laterally spreading tumors(LST)do not adhere to any of these models and exhibit extremely serious progression and poor *** this article,we present another possible pathway involved in CRC development,particularly from LST,with important molecular characteristics,which would facilitate the design of a novel strategy for targeted therapy.

关键词： Colorectal cancer Laterally spreading tumors Molecular mechanism Truncated adenomatous polyposis coli mutation Golgi fragmentation Cancerous mechanism

The dying accretion and jet in a powerful radio galaxy of Hercules A

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2020年第8期20卷 193-200页

作者： Lin-Hui Wu Qing-Wen Wu Jian-Chao Feng Ru-sen lu Xu-Liang Fan School of Physics Huazhong University of Science and TechnologyWuhan 430074China School of Physics and Electronic Science Guizhou Normal UniversityGuiyang 550001China Shanghai Astronomical Observatory Chinese Academy of ScienceShanghai 200030China Max-Planck-Institut fuir Radioastronomie Auf dem Higel 69D-53121 BonnGermany School of Mathematics Physics and StatisticsShanghai University of Engineering ScienceShanghai 201620China

Hercules A(Her A)is one of a rare class of dying and transition-type objects,which has a pair of giant,powerful radio lobes and a weak radio *** reduce and analyze the radio data of Her A that were observed by the Expanded Very Large Array(EVLA)during 2010-2011 at C *** intensity distribution is very smooth along the edge of the lobe front and the intensity also sharply decreases at the edge,which supports that magnetic fields may play an important role in radio *** spectrum of the weak core is very steep and the core flux becomes weaker by about ten percent when compared to what was observed twenty years ago,which suggest that the central engine is still dying *** A deviates a lot from the relation between[O III]luminosity and low-frequency 178 MHz luminosity(LO III-L178 MHz)as defined by other FR I/II ***,when only radio core emission is considered,it roughly follows an LO III-L178 *** result supports that the black-hole accretion and large-scale jet in Her A did not evolve simultaneously,and indicates that although the large-scale jet is still powerful,the accretion and inner jet have changed into an inactive *** on the estimated Bondi accretion rate,we model the spectrum of Her A with a radiatively inefficient accretion flow and jet model.

关键词： black hole physics galaxies:jets radiation mechanisms:non-thermal galaxies:magnetic fields radio continuum:galaxies

Immunological inhibition of transplanted liver allografts by adeno-associated virus vector encoding CTLA4Ig in rats

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2008年第3期7卷 258-263页

作者： lu, sen Yu, Yue Gao, Yun Li, Guo-Qiang Wang, Xue-Hao Nanjing Med Univ Ctr Liver Transplantat Affiliated Hosp 1 Nanjing 210029 Peoples R China

BACKGROUND: Blockade interaction between CD28 and B7 with CTLA4Ig has been shown to induce experimental transplantation tolerance. In order to prolong the inhibitory effect of CTLA4Ig, a recombinant adeno-associated virus vector pSNAV expressing CTLA4Ig was constructed, and its effects on transplanted liver allografts were investigated. METHODS: The pSNAV-CTLA4Ig construct was infused into partial liver allografts of rats via the portal vein during transplantation. CTLA4Ig expression in the transplanted livers was detected with reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and immunohistochemistry. Furthermore, real-time quantitative PCR was used to measure the expression of IL-2, IFN-gamma, IL-4 and IL-10 in the allografts. RESULTS: The expression of CTLA4Ig in the partial allograft was detected successfully and pSNAV-CTLA4Ig improved the survival rate of rats after liver transplantation. Agarose gel analysis of RT-PCR products indicated the presence of CTLA4Ig in the pSNAV-CTLA4Ig treatment group. Cytokines expressed in allografts on day 7 after orthotopic liver transplantation showed that IL-2, IFN-gamma, IL-4 and IL-10 mRNA levels decreased in transplant recipients treated with pSNAV-CTLA4Ig compared with those treated with pSNAV-LacZ (1.62 +/- 0.09,1.52 +/- 0.11,1.50 +/- 0.07 and 1.43 +/- 0.07 versus 1.29 +/- 0.09, 1.32 +/- 0.07, 1.34 +/- 0.06 and 1.35 +/- 0.04, respectively). CONCluSIONS: pSNAV-CTLA4Ig effectively expressed CTLA4Ig in liver allografts. CTLA4Ig improved the pathological findings after liver transplantation. CTLA4Ig induced immune tolerance of liver transplantation, and the mechanism involved induced alteration of Th1 and Th2 cytokine transcripts. The adeno-associated virus vector encoding CTLA4Ig may be useful in the clinical study of transplantation tolerance.

关键词： cTLA4Ig adeno-associated virus liver transplantation transplantation tolerance

Synthesis,bioactivities and 3D-QSAR of novel avermectin B2a aglycon derivatives

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2020年第1期31卷 141-144页

作者： Fangfang Huang Huan Ling Jiao Li sen lu Weijie Liu Qingshan Li Fengbo Xu State Key Laboratory of Elemento-Organic Chemistry Department of ChemistryCollege of ChemistryNankai University

Fourteen avermectin B2 a aglycon derivatives were designed and synthesized after removing the oleandrose disaccharide of avermectin B2 *** structures were characterized by’H NMR,13 C NMR,*** bioassays indicated that these compounds exhibited good insecticidal activity against diamondback moth at 200 mg/L,with mortality no less than 90%.Compounds 10 b,12 a,12 c,17 demonstrated good acaricidal activity against the adult mites,larvae,and good inhibition rate of hatching to mite eggs of Tetranychus *** 5,10 b,10 c exhibited excellent fungicidal activity against fourteen fungal pathogens in vitro.3 D-QSAR analysis showed that the fungicidal activity of avermectin B2 a aglycon derivatives would be increased when a negatively charged and bulky group was introduced at 13-position,which will be instructive for the further modification of avermectin B2 aaglycon.

关键词： Avermectin B2a aglycon Biological activity Insecticide Acaricide Fungicide 3D-QSAR

MAPPING OF THE RICE (ORYZA SATIVA L.) THERMO-senSITIVE GENIC MALE STERILE GENE TMS5 WITH EST AND SSR MARKERS

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2006年第4期51卷 417-420页

作者： JIANG Dagang lu sen ZHOU Hai WU Xiaojin ZHUANG Chuxiong LIU Yaoguang Key Laboratory of Plant Functional Genomics and Biotechnology ofGuangdong Province College of Life Scienc South China Agricul-tural University Guangzhou 510642 China National Hybrid Rice Engineering and Technique Research Center Changsha 410125 China

With the cDNA suppression subtraction hybridization method, a spikelet-specific cDNA library was constructed that expressed at meiosis stage in rice. A total of 121 cDNA fragments were selected from the library and used as EST (expressed se-quence tags) markers to detect the polymorphism between Annong N, a normal fertile Indica rice line and Annong S-1, its spontaneous mutant with thermo-sensitive genic male sterility, using the RFLP (restriction fragment length polymorphism) technique. HN57, one of the EST probes, could detect poly-morphism between them. The results of segregation analysis with the F2 population developed from An-nong S-1 and Annong N indicate that HN57 co-seg- regates with the thermo-sensitive genic male-sterility controlled by tms5, the recessive gene in Annong S-1. This marker is located on the 31.2-cM region of the chromosome 2 of RGP (rice genome research pro-gram) genetic map. To further determine the location of tms5, 80 SSR (simple sequence repeat) markers around this region were developed, and 12 of them were polymorphic. And finally, the tms5 was mapped within region of 181 kb by using these new markers.

关键词：水稻热敏感基因雄性不育基因 tms5基因杂交育种