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Zoonotic transmission of hepatitis E:Implications for public health worldwide

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Asian Pacific Journal of Tropical Medicine 2009年第4期2卷 77-82页

作者： Analia paiva Victoria Rebagliati Alcides Troncoso department of Microbiology Parasitology and Infectious DiseasesSchool of MedicineUniversity of Buenos AiresArgentina

Since the year 1982,the Hepatitis E virus(HEV),enteric transmission,has been widely spread in many tropical and subtropical *** has multiple routes of transmission,such as food or water contaminated with faecal matter,person-to-person transmission or by the ingestion of raw or undercooked *** is important to consider the significance of zoonotic infections propagation,both in endemic areas as well as in developed countries,where there have been some sporadic *** article summarizes the research conducted to date,which supports the zoonotic transmission of HEV,its risks and how they are often underestimated.

关键词： Human zoonosis HEV Public health

Utilization of Acai Berry Residual Biomass for Extraction of Lignocellulosic Byproducts

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Journal of Bioresources and Bioproducts 2021年第4期6卷 323-337页

作者： Lamia Zuniga Linan Anne C.Mendonça Cidreira Cláudia Quintino da Rocha Fabrícia Farias de Menezes George J de Moraes Rocha Antônio E Macedo paiva departamento de Engenharia Química Universidade Federal do Maranhao(UFMA)Av.dos Portugueses 1966Bacanga-CEP65080-805Sao Luís-MABrazil departamento de Química Universidade Federal do Maranhao(UFMA)Av.dos Portugueses 1966Bacanga-CEP65080-805Sao Luís-MABrazil Brazilian Center for Research in Energy and Materials(CNPEM)and Brazilian Biorenewables National Laboratory(LNBR) Giuseppe Máximo Scolfaro 10.000Guará-CEP13083-100 Campinas-SPBrazil departamento de Mecânica e Materiais(DMM) Instituto Federal de EducaçãoCiência e Tecnologia do maranhão(IFMA)São Luís-MABrazil

According to the National Company of Supplying(CONAB)in 2017 alone,the national production of acai pulp reached 219855 t,equating to 180 million dollar(USD).Almost 85%of the weight of fruit is constituted by residual biomass,even though researches have highlighted important applications for this biomass,most of it is discarded as organic ***,it is relevant to envisage in-depth studies about how to use these residues,particularly regarding the environmental impact of its target *** cellulose(CNC)and lignin are organic derivatives obtained through the physical-chemical treatment of lignocellulosic *** are abundant and currently considered as biopolymers because of their structural characteristics and their diverse applications in food and the medical *** work presents the mass yields achieved and the physical-chemical characteristics of the lignocellulosic derivatives extracted from the fiber of the acai berry.A statistical design was used to define the influence of process variables as temperature,reaction time and fiber size on the yield of these byproducts.A maximum yield close to 64%of type I CNC,with 45%of crystallinity degree was achieved at the minimum condition of temperature and fiber ***,through rheological analysis,it was possible to predict the nanocrystal aspect ratios,ranging from 71 to *** extracted lignin was rich in methoxy groups,p-coumaryl alcohol and p-coumaric acid,and its structural unit’s low state of aggregation can be an indication of low molecular weight,which envisions an appropriate use for this lignin to produce commodity chemicals.

关键词： Acai berry Residual biomass Cellulose nanocrystal(CNC) Lignin rheological characterization Physical-chemical characterization

Loss of oral mucosal stem cell markers in oral submucous fibrosis and their reactivation in malignant transformation

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International Journal of Oral Science 2020年第3期12卷 199-208页

作者： Mohit Sharma Felipe paiva Fonseca Keith D.Hunter Raghu Radhakrishnan department of Oral Pathology Sudha Rustagi College of Dental Sciences and ResearchFaridabadHaryanaIndia department of Oral Surgery and Pathology School of DentistryFederal University of Minas GeraisBelo HorizonteMGBrazil Academic Unit of Oral and Maxillofacial Medicine and Pathology School of Clinical DentistryUniversity of SheffieldClaremont CrescentSheffieldUK department of Oral Pathology Manipal College of Dental SciencesManipal Academy of Higher EducationManipalKarnatakaIndia

The integrity of the basal stem cell layer is critical for epithelial *** this paper,we review the expression of oral mucosal stem cell markers(OM-SCMs)in oral submucous fibrosis(OSF),oral potentially malignant disorders(OPMDs)and oral squamous cell carcinoma(OSCC)to understand the role of basal cells in potentiating cancer stem cell behaviour in *** the loss of basal cell clonogenicity triggers epithelial atrophy in OSF,the transition of the epithelium from atrophic to hyperplastic and eventually neoplastic involves the reactivation of basal *** vacillating expression patterns of OM-SCMs confirm the role of keratins 5,14,19,CD44,β1-integrin,p63,sex-determining region Y box(SOX2),octamer-binding transcription factor 4(Oct-4),c-MYC,B-cell-specific Moloney murine leukaemia virus integration site 1(Bmi-1)and aldehyde dehydrogenase 1(ALDH1)in OSF,OPMDs and *** downregulation of OM-SCMs in the atrophic epithelium of OSF and their upregulation during malignant transformation are illustrated with relevant literature in this review.

关键词： epithelium malignant mucosal

Mitigation of Greenhouse Gas Emissions from Tropical Soils Amended with Poultry Manure and Sugar Cane Straw Biochars

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Agricultural Sciences 2017年第9期8卷 887-903页

作者： sarah Vieira Novais Mariana delgado oliveira Zenero Elizio Ferreira Frade Junior Renato paiva de Lima Carlos Eduardo Pelegrino Cerri department of Soil Science Escola Superior de Agricultura Luiz de Queiroz University of S&#227 o Paulo Piracicaba Brazil department of Environmental Sciences Federal Institute of Mato Grosso Barra do Gar&#231 a Brazil

Increases in greenhouse gases (GHG) emissions, upon changes in land use and agricultural management, lead to a search for techniques that enhance carbon residence time in soil. Pyrolysis increases the recalcitrance of organic materials and enhances their activities as physical, chemical and biological soil conditioners. Emissions of CO2, CH4 and N2O quantified from a sandy soil that was treated with three rates (12.5, 25 e 50 Mg·ha-1) of either non-pyrolysed poultry manure and sugarcane straw or biochars, pyrolysed at two contrasting temperatures (350°C and 650°C). Subsequently, the flux of the three gases was converted and compared in a standard unit (CO2eq). The added biochars, significantly reduced GHG emissions, especially CO2, relative to the non-pyrolysed materials. The greatest differences between applied rates of poultry manure, relative sugarcane straw, both to biochar and raw material, and the positive response to the increase of pyrolysis temperture, confirm the importance of raw material choice for biochar production, with recalcitrance being an important initial characteristic. Greater emissions occurred with intermediate rate of biochars (25 Mg·ha-1) amendment to the soil. These intermediate rates had higher microbial biomass, provided by an intermediate C/N ratio derived from the original soil and the biochar, promoting combined levels of labile C and oxygen availability, leading to an optimal environment for microbiota.

关键词： CO2 CH4 N2O Weathered Soil

Clinical and environmental isolates of Burkholderia pseudomallei from Brazil: Genotyping and detection of virulence gene

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Asian Pacific Journal of Tropical Medicine 2017年第10期10卷 945-951页

作者： Tereza de Jesus Pinheiro Gomes Bandeira debora de Souza Collares Maia Castelo-Branco Marcos Fabio Gadelha Rocha Rossana de Aguiar Cordeiro Crister Jose Ocadaque Manoel de Araújo Neto paiva Raimunda samia Nogueira Brilhante Jose Júlio Costa Sidrim Specialized Medical Mycology Center Postgraduate Program in Medical Microbiology Federal University of Ceará Christus School of Medicine Postgraduate Program in Veterinary Sciences State University of Ceará Federal Institute of Education Science and Technology of Ceará

Objective: To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei(***) recovered in Ceara, Brazil, and screen these isolates for the presence of type three secretion system virulence ***: Nineteen *** isolates(9 from clinical cases and 10 from soils)were *** amplified polymorphic DNA was performed with primers OPQ-2, OPQ-4 and OPQ-16 to evaluate the genetic diversity, and type three secretion system gene was detected through polymerase chain ***: Random amplified polymorphic DNA showed a genetic relatedness of approximately 50% among the tested *** isolates, which were grouped into two clades, of which the biggest ones comprised 18/19 isolates for primer OPQ-2, and 17/19 isolates for primer *** OPQ-4 grouped the isolates into three clades comprising 1/19, 3/19 and 15/19 ***, type three secretion system gene was detected in all tested ***: This is an effort to type *** strains from Ceara, which is important for better understanding this pathogen, contributing for the epidemiological surveillance of melioidosis in this endemic region.

关键词： Burkholderia pseudomallei Melioidosis RAPD TTSS gene

Risk stratification of patients with atrial fibrillation: Biomarkers and other future perspectives

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World Journal of Cardiology 2012年第6期4卷 195-200页

作者： Rui Providência luís paiva Sérgio Barra department of Cardiology Coimbra’s Hospital Centre and University 3041-801 S.Martinho do Bispo Coimbra Portugal Coimbra’s Medical School University of Coimbra 3041-801 S.Martinho do Bispo Coimbra Portugal

Risk stratification of atrial fibrillation (AF) and adequate thromboembolism prophylaxis is the cornerstone of treatment in patients with AF. Current risk stratification schemes such as the CHADS 2 and CHA 2 DS 2 -VASc scores are based on clinical risk factors and suboptimally weight the risk/benefit of anticoagulation. Recently, the potential of biomarkers (troponin and NT-proBNP) in the RE-LY biomarker sub-analysis has been demonstrated. Echocardiography is also being evaluated as a possible approach to improve risk score performance. The authors present an overview on AF risk stratification and discuss future potential developments that may be introduced into our current risk stratification schemes.

关键词： Anticoagulation Atrial fibrillation Risk stratification Stroke Thromboembolism

Experimental evidence for heritable reproductive time in 2 allochronic populations of pine processionary moth

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Insect Science 2017年第2期24卷 325-335页

作者： Manuela Branco Maria-Rosa paiva Helena Maria santos Christian Burban Carole Kerdelhue Centro de Estudos Florestais (CEF) Instituto Superior de Agronomia (ISA) Universidade de Lisboa (ULisboa) 1349-017 Lisboa Portugal CENSE Faculty of Sciences and Technology (FCT) Universidade Nova de Lisboa (UNL) 2829-516 Caparica Portugal INRA Centre de Bordeaux UMR1202 BIOGECO Cestas cedex France INRA Centre de Montpellier UMR CBGP F-34988 Montferrier-sur-Lez cedex France

Phenology allows organisms to overcome seasonally variable conditions through life-cycle adjustment. Changes in phenology can drastically modify the evolutionary tra- jectory of a population, while a shift in the reproductive time may cause allochronic differentiation. The hypothesis of heritable reproductive time was experimentally tested, by studying a unique population of the pine processionary moth Thaumetopoeapityocampa （den. ＆ Schiff.） which has a shifted phenology, and however co-occurs with the typical population following the classical life cycle. When populations of both types were reared under controlled conditions, the reproductive time was maintained asynchronous, as ob- served in the field. The shifted population was manipulated in the laboratory to reproduce later than usual, yet the offspring emerged in the next year at the expected dates thus ＂com- ing back＂ to the usual cycle. Hybrids from crosses performed between the 2 populations showed an intermediate phenology. From the emergence times of parents and offspring, a high heritability of the reproductive time （h = 0.76） was observed. The offspring ob- tained from each type of cross was genetically characterized using microsatellite markers. Bayesian clustering analysis confirmed that hybrids can he successfully identified and separated from the parental genetic classes by genotyping. Findings support the hypothesis that, for this particular population, incipient allochronic speciation is due to a heritable shift in the reproductive time that further causes assortative mating and might eventually cause ecological adaptation/maladaptation in response to environmental changes.

关键词： allochronic speciation experimental crossings heritability phenology reproductive isolation Thaumetopoeapityocampa

Prediction of saRS-CoV-2 hosts among Brazilian mammals and new coronavirus transmission chain using evolutionary bioinformatics

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Animal Diseases 2022年第1期2卷 16-26页

作者： luciano Rodrigo Lopes Giancarlo de Mattos Cardillo Natalia Carvalho de lucca Pina Antonio Carlos da Silva Junior Silvana Kertzer Kasinski Paulo Bandiera-paiva SPBrasil SPBrasil

Severe acute respiratory syndrome coronavirus(saRS-CoV)and saRS-CoV-2 are thought to transmit to humans via wild mammals,especially ***,evidence for direct bat-to-human transmission is *** of intermediate hosts is considered a reason for saRS-CoV-2 transmission to humans and emergence of *** biodiversity is found in tropical territories,such as *** the similar line,this study aimed to predict potential coronavirus hosts among Brazilian wild mammals based on angiotensin-converting enzyme 2(ACE2)sequences using evolutionary ***,maned wolf,and bush dogs were predicted as potential hosts for *** indigenous carnivores are philogenetically closer to the known saRS-CoV/saRS-CoV-2 hosts and presented low ACE2 divergence.A new coronavirus transmission chain was developed in which white-tailed deer,a susceptible saRS-CoV-2 host,have the central *** play an important role because of its low divergent ACE2 level in deer and *** discovery of these potential coronavirus hosts will be useful for epidemiological surveillance and discovery of interventions that can contribute to break the transmission chain.

关键词： saRS-CoV-2 Angiotensin-converting enzyme 2 Coronavirus Brazilian mammals White-tailed deer

Complete Achilles Tenotomy:A New Improved Experimental Surgical Technique in Rats

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Open Journal of Animal Sciences 2018年第1期8卷 1-11页

作者： Kelly Biancardini Gomes Barbato Guilherme de Almeida Joao da Costa Libardo Rodriguez Clara Raposo Hanna Dias Renata paiva Liszt Palmeira de oliveira Jorge Carvalho Clinical and Experimental Pathophysiology Postgraduate Program School of MedicineState University of Rio de JaneiroRio de JaneiroBrazil department of Surgical Specialties School of MedicineState University of Rio de JaneiroRio de JaneiroBrazil School of Medicine State University of Rio de JaneiroRio de JaneiroBrazil department of Histology and Embriology Roberto Alcantara Gomes Institute of BiologyState University of Rio de JaneiroRio de JaneiroBrazil

Rupture of the Achilles tendon is one of the most frequent tendon injuries in humans. However, there is no standard surgical technique thus far to reproduce complete rupture of this tendon in rats. Surgical procedures were performed in Wistar rats under light microscopy, with posterior longitudinal incision and preservation of the plantaris tendon. The animals were sacrificed on 2nd and 8th postoperative week and we used hematoxilin and eosin, picrosirius-red and Weigert’s resorcin-fuchsin to observe general tendon healing. The mean operative time was one minute and 24 seconds. Histological studies showed cellular proliferation and fibrillogenesis at two weeks, with decreased amounts of cellularity and elastic fibers at the 8th week, besides changes in structural organization of collagen fibers. The complete Achilles tenotomy in rats, was improved under light microscopy and sparing the plantaris tendon. This surgical technique was simple and quick, showed signs of normal healing process, could be easily reproducible, and triggered translational researches in this area.

关键词： Achilles Tendon Rupture Animal Experimentation Wound Healing Collagen Elastic Tissue