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Journal of Oceanology and Limnology 2024年第1期42卷 216-225页

作者： Zhongyi ZHANG Hang liu Xiaohui PAN Yanan ZONG Leili FENG lixian liu Li GUO Guanpin YANG College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Institutes of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of ChinaQingdao 266003China

Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from *** genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-strand breaks of DNA,causing insertional *** random insertional mutant library constructed using this method has become a method of forward genetics for gene ***,the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous *** microalgal species show a low transformation efficiency,making constructing random insertional mutant libraries *** this study,we established a highly efficient transformation method for constructing a random insertional mutant library of Nannochloropsis oceanica,and tentatively tried to isolate its genes to prove the feasibility of the method.A gene that may control the growth rate and cell size was *** method will facilitate the genetic studies of ***,which should also be a reference for other microalgal species.

关键词： Nannochloropsis oceanica genetic transformation random insertional mutant library zeocin pretreatment forward genetics

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设计政策引领下的英国时尚产业转型与发展

丝绸 2024年第6期61卷 79-86页

作者：刘丽娴李樾卢益婧浙江省丝绸与时尚文化研究中心杭州310018 浙江理工大学服装学院杭州311199 浙江理工大学国际时装技术学院杭州311199

回望历史,设计政策的萌芽可追溯至1851年“水晶宫博览会”的筹办,虽然整体看来在19世纪末以来英国时尚以追随巴黎为主,但是英国政府在其时尚产业历经多次危机期间积极作为,主要为“二战”后维护传统至寻求创新创意的转变;历史积淀加之... 详细信息

回望历史,设计政策的萌芽可追溯至1851年“水晶宫博览会”的筹办,虽然整体看来在19世纪末以来英国时尚以追随巴黎为主,但是英国政府在其时尚产业历经多次危机期间积极作为,主要为“二战”后维护传统至寻求创新创意的转变;历史积淀加之关注创意文化引发英国时尚转型步入创意发展阶段,并将时尚纳入“创意文化产业”范畴;全球“疫情”加速英国设计与时尚品牌呈现数字化转型趋势,并催生可持续发展背景下的新型时尚产业生态链路。文章通过比对设计政策及其引领下的英国时尚产业发展历史阶段,以期通过历史样本的再确认探讨其所映射的中国价值。

关键词：设计管理设计政策英国时尚时尚产业时尚产业转型创意文化产业

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基于大数据技术的服装智能制造创新模式

针织工业 2024年第2期 49-54页

作者：刘丽娴王贺林郑泽宇向忠浙江理工大学国际时装技术学院浙江杭州311199 浙江理工大学服装学院浙江杭州311199 杭州知衣科技有限公司浙江杭州311200 浙江理工大学机械工程学院浙江杭州311200

为进一步提高服装行业的智能化水平,在对目前服装行业面临的智能制造建设任务分析的基础上,探讨大数据技术在数字经济时代助力服装行业转型升级实现智能化过程中所发挥的关键作用。通过梳理大数据技术在服装流行趋势智能预测、数字化设... 详细信息

为进一步提高服装行业的智能化水平,在对目前服装行业面临的智能制造建设任务分析的基础上,探讨大数据技术在数字经济时代助力服装行业转型升级实现智能化过程中所发挥的关键作用。通过梳理大数据技术在服装流行趋势智能预测、数字化设计、智慧工厂生产、个性化营销4个方面的现有研究,提出由大数据技术驱动的服装智能制造创新模式,探讨从预测、设计、生产到销售整个闭环的智能化制造流程,以及基于大数据技术的服装智能制造发展趋势。

关键词：大数据技术智能制造服装行业智能化创新模式

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地表增荷比对下方盾构隧道变形和内力的影响

工业安全与环保 2024年第8期50卷 61-65页

作者：丁昊玉刘利先李霖昆明理工大学建筑工程学院云南昆明650500

在盾构隧道上方施工时很容易引发地表产生额外荷载,进而对隧道结构产生不利影响。针对此问题,以昆明地铁1号线某区间盾构隧道为工程实例,利用MIDAS GTS NX有限元软件,建立二维双线隧道模型,提出增荷比这一概念,深入研究了增荷比对下方... 详细信息

在盾构隧道上方施工时很容易引发地表产生额外荷载,进而对隧道结构产生不利影响。针对此问题,以昆明地铁1号线某区间盾构隧道为工程实例,利用MIDAS GTS NX有限元软件,建立二维双线隧道模型,提出增荷比这一概念,深入研究了增荷比对下方盾构隧道变形和内力的影响。结果表明:地表增荷作用下,拱腰处承受最大轴力,拱顶其次,拱底轴力最小;隧道正截面上下左右4点处弯矩值相较其他位置更大且趋于接近,拱顶处弯矩值最大;随着增荷比的增大,隧道拱顶、拱底、左右拱腰处的受力状态由小偏压转变为大偏压,且拱顶处偏心距最大;隧道的竖向位移及内力皆呈线性递增趋势,当增荷比为12%时,最大竖向位移已经接近沉降控制值。

关键词：盾构隧道增荷比变形内力偏心距椭圆度

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新昌调腔戏曲服饰研究

染整技术 2024年第4期46卷 75-78页

作者：王硕卢益婧刘丽娴浙江理工大学丝绸与时尚文化研究中心浙江杭州310018

新昌调腔戏曲服饰作为戏曲的重要组成部分之一,是新昌调腔的重要象征符号。基于现有文献资料的整理,研究新昌调腔的起源、曲目以及风格,分析新昌调腔服饰的形制、色彩、纹样、面料与品类。在关注中华优秀传统文化与非物质文化遗产的当下... 详细信息

新昌调腔戏曲服饰作为戏曲的重要组成部分之一,是新昌调腔的重要象征符号。基于现有文献资料的整理,研究新昌调腔的起源、曲目以及风格,分析新昌调腔服饰的形制、色彩、纹样、面料与品类。在关注中华优秀传统文化与非物质文化遗产的当下,有关新昌调腔及其服饰的研究具有历史与当代价值。

关键词：新昌调腔戏曲服饰非遗传承

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借鉴元理论的时尚研究

服装设计师 2024年第9期 94-97页

作者：胡可刘丽娴穆琛浙江理工大学艺术与设计学院

时尚本身的综合属性决定了其通过跨学科理论借鉴展开研究的必要与必然性,借鉴德国社会学家、哲学家卢曼的社会系统理论,界定时尚研究的边界。基于元理论,从两个视角展开时尚研究,一是“从一”与“从兀”,二是自我指涉与系统概念。

关键词：元理论时尚社会系统理论自我指涉

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Investigation of Optimal Megnetic Properties in NdFeB Magnets by Artificial Neural Network

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Journal of Rare Earths 2004年第z1期22卷 57-62页

作者： Lian lixian liu Ying Hu Wang Hou Tinghong Gao Shengji Tu Mingjing College of Materials Science & Engineering Sichuan University Chengdu 610065 China College of Materials Science & Engineering Sichuan University Chengdu 610065 China School of Computer Science & Engineering Sichuan University Chengdu 610065 China College of Materials Science & Engineering Sichuan University Chengdu 610065 China College of Materials Science & Engineering Sichuan University Chengdu 610065 China College of Materials Science & Engineering Sichuan University Chengdu 610065 China

In order to study the effect of alloy component on magnetic properties of NdFeB magnets, the experiment schemes are carried out by the uniform design theory, and the relationship between the component and the magnetic properties is established by artificial neural network(ANN) predicting *** element contents of alloys are optimized by the ANN ***, the influences of mono-factor or multi-factor interaction on alloy magnetic properties are respectively discussed according to the curves ploted by ANN *** result shows that the predicted and measured results are in good *** relative error is every low, the error is not more than 1.68% for remanence Br, 1.56% for maximal energy product (BH)m, and 7.73% for coercivity *** can be obviously improved and Br can be reduced by increasing Nd or Zr *** and B have advantageous effects on increasing Br and disadvantageous effects on increasing *** of alloying elements on Hcj and Br are inverse, and the interaction among the alloying elements play an important role in the magnetic properties of NdFeB *** ANN prediction model presents a new approach to investigate the nonlinear relationship between the component and the magnetic properties of NdFeB alloys.

关键词： metal materials artificial neural network uniform design NdFeB magnetic properties rare earths

医护一体化护理对冠心病患者PCI术后心功能及心血管不良事件发生率的影响

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临床医学工程 2024年第6期31卷 745-746页

作者：冷本芹李艳霞郭晓燕刘利贤新乡市中心医院(新乡医学院第四临床学院)心血管内科河南新乡457000

目的探讨医护一体化护理对冠心病患者经皮冠状动脉介入(PCI)术后心功能及主要心血管不良事件(MACE)发生率的影响。方法选取2020年6月至2022年6月于我院接受PCI治疗的86例冠心病患者,随机分为两组各43例。术后,对照组予以常规护理,观察... 详细信息

目的探讨医护一体化护理对冠心病患者经皮冠状动脉介入(PCI)术后心功能及主要心血管不良事件(MACE)发生率的影响。方法选取2020年6月至2022年6月于我院接受PCI治疗的86例冠心病患者,随机分为两组各43例。术后,对照组予以常规护理,观察组予以医护一体化护理。比较两组的心功能和MACE发生情况。结果护理后,观察组的LVESD、LVEDD均显著低于对照组,LVEF显著高于对照组(P<0.05)。观察组的MACE发生率为4.65%,显著低于对照组的20.93%(P<0.05)。结论医护一体化护理可有效恢复冠心病患者PCI术后的心功能,减少MACE的发生。

关键词：冠心病医护一体化护理心功能主要心血管不良事件

Matrix metalloproteinase 2 contributes to adult eclosion and immune response in the small hive beetle,Aethina tumida

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Insect Science 2024年第3期31卷 733-747页

作者： lixian Wu Yajing Xu Liangbin Li Dainan Cao Fang liu Hongxia Zhao Guangdong Key Laboratssory of Animal Conservation and Resource Utilization Guangdong Public Laboratory of Wild Animal Conservation and UtilizationInstitute of ZoologyGuangdong Academy of SciencesGuangzhouChina

During the pupal-adult eclosion process of holometabolous insects,the old cuticle is shed and replaced by a completely different new cuticle that requires tanning and expansion,along with extensive extracellular matrix(ECM)*** vertebrates,matrix metalloproteinases(MMPs),a class of zinc-dependent endopeptidases,play key roles in regulating the ECM that surrounds ***,little is known about these extracellular proteinases available in *** small hive beetle(SHB),Aethina tumida,is a widespread invasive parasite of honey *** this study,6 MMP homologs were identified in the SHB *** interference experiments showed that all 6 AtMmps are not required for the larval-pupal transition,only AtMmp2 was essential for pupal-adult eclosion in *** of AtMmp2 resulted in eclosion defects and wing expansion failure,as well as mortality within 3 d of adult *** analysis revealed that knockdown of AtMmp2 significantly increased expression of the Toll and Imd pathways,chitin metabolism,and cross-linking(such as the pro-phenoloxidase activating cascade pathway and the tyrosine-mediated cuticle sclerotization and pigmentation pathway).These data revealed evolutionarily conserved functions of Mmp2 in controlling adult eclosion and wing expansion,also provided a preliminary exploration of the novel function of regulating Toll and Imd pathways,as well as new insights into how MMPs regulate insect development and defense barriers.

关键词： Aethina tumida adult eclosion immune response matrix metalloproteinase RNAinterference

Associations of genome-wide structural variations with phenotypic differences in cross-bred Eurasian pigs

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Journal of Animal Science and Biotechnology 2024年第1期15卷 68-87页

作者： Wencheng Zong Jinbu Wang Runze Zhao Naiqi Niu Yanfang Su Ziping Hu Xin liu Xinhua Hou Ligang Wang lixian Wang Longchao Zhang State Key Laboratory of Animal Biotech Breeding Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China College of Animal Science Shanxi Agricultural UniversityJinzhong 030801China College of Animal Science and Technology Qingdao Agricultural UniversityQingdao 266109China

Background During approximately 10,000 years of domestication and selection,a large number of structural variations(SVs)have emerged in the genome of pig breeds,profoundly influencing their phenotypes and the ability to adapt to the local ***(≥50 bp)are widely distributed in the genome,mainly in the form of insertion(INS),mobile element insertion(MEI),deletion(DEL),duplication(DUP),inversion(INV),and translocation(TRA).While studies have investigated the SVs in pig genomes,genome-wide association studies(GWAS)-based on SVs have been rarely *** Here,we obtained a high-quality SV map containing 123,151 SVs from 15 Large White and 15 Min pigs through integrating the power of several SV tools,with 53.95%of the SVs being reported for the first *** high-quality SVs were used to recover the population genetic structure,confirming the accuracy of *** functional SV loci were then identified based on positional effects and breed ***,GWAS were performed for 36 traits by genotyping the screened potential causal loci in the F2 population according to their corresponding genomic *** identified a large number of loci involved in 8 carcass traits and 6 skeletal traits on chromosome 7,with FKBP5 containing the most significant SV locus for almost all *** addition,we found several significant loci in intramuscular fat,abdominal circumference,heart weight,and liver weight,*** We constructed a high-quality SV map using high-coverage sequencing data and then analyzed them by performing GWAS for 25 carcass traits,7 skeletal traits,and 4 meat quality traits to determine that SVs may affect body size between European and Chinese pig breeds.

关键词： Body size GWAS Pig Skeleton Structural variations