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Journal of Rock Mechanics and Geotechnical Engineering 2022年第6期14卷 2034-2057页

作者： liwang liu Haibo Li Xiaofeng Li State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China

The mechanical characteristics and failure behavior of rocks containing flaws or discontinuities have received wide attention in the field of rock *** external loads are applied to rock materials,stress-induced cracks would initiate and propagate from the flaws,ultimately leading to the irreversible failure of *** investigate the cracking behavior and the effect of flaw geometries on the mechanical properties of rock materials,a series of samples containing one,two and multiple flaws have been widely investigated in the *** this paper,the experimental results for pre-cracked rocks under quasistatic compression were systematically *** progressive failure process of intact rocks is briefly described to reveal the background for experiments on samples with ***,the nondestructive measurement techniques utilized in experiments,such as acoustic emission(AE),X-ray computed tomography(CT),and digital image correlation(DIC),are *** mechanical characteristics of rocks with different flaw geometries and under different loading conditions,including the geometry of pre-existing flaws,flaw filling condition and confining pressure,are ***,the cracking process is evaluated from the perspective of crack initiation,coalescence,and failure patterns.

关键词： Pre-cracked rock Crack initiation pattern Crack coalescence pattern Failure pattern

Effect of intermittent joint distribution on the mechanical and acoustic behavior of rock masses

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第4期16卷 1231-1244页

作者： Shuaiyang Fu Haibo Li liwang liu Di Wu Ben Wang State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China

The mechanical characteristics and acoustic behavior of rock masses are greatly influenced by stochastic *** this study,numerical models of rock masses incorporating intermittent joints with different numbers and dip angles were produced using the finite element method(FEM)with the intrinsic cohesive zone model(ICZM).Then,the uniaxial compressive and wave propagation simulations were *** results indicate that the joint number and dip angle can affect the mechanical and acoustic properties of the *** uniaxial compressive strength(UCS)and wave velocity of rock masses decrease monotonically as the joint number ***,the wave velocity grows monotonically as the joint dip angle *** the joint dip angle is 45°–60°,the UCS of the rock mass is lower than that of other dip *** wave velocity parallel to the joints is greater than that perpendicular to the *** the dip angle of joints remains unchanged,the UCS and wave velocity are positively *** the joint dip angle increases,the variation amplitude of the UCS regarding the wave velocity *** reveal the effect of the joint distribution on the velocity,a theoretical model was also *** to the theoretical wave velocity,the change in wave velocity of models with various joint numbers and dip angles was consistent with the simulation ***,a theoretical indicator(*** tensor)was adopted to analyze the variation of the wave velocity and UCS.

关键词： Stochastic joints Intrinsic cohesive zone model Uniaxial compressive strength(UCS) Wave propagation Fabric tensor

Crystallization and Luminescence Properties of Sm3＋-Doped SrO-Al2O3-SiO2 Glass-Ceramics

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Journal of Wuhan University of Technology(Materials Science) 2017年第5期32卷 1025-1031页

作者：李宏 liu liwang TANG Xiaozhe WANG Qian Paul W Wang 王伟 State Key Laboratory of Silicate Materials for Architectures (Wuhan University of Technology) Wuhan 430070 China Department of Physics Bradley University Peoria 10 6162.5 USA School of Automotive Engineering Wuhan University of Technology Wuhan 430070 China Hubei Province Key Laboratory of Modern Automotive Technology Wuhan 430070 China 5.Hubei Province Automotive Parts Synergy Innovation Center Wuhan 430070 China

The Sm^（3＋）-doped SrO-Al2O3-SiO2（SAS） glass-ceramics with excellent luminescence properties were prepared by batch melting and heat treatment. The crystallization behavior and luminescent properties of the glass-ceramics were investigated by DTA, XRD, SEM and luminescence spectroscopy. The results indicate that the crystal phase precipitated in this system is monocelsian（SrAl2Si2O） and with the increase of nucleation/crystallization temperature, the crystallite increases from 66 % to 79 %. The Sm（3＋）-doped SAS glass-ceramics emit green, orange and red lights centered at 565, 605, 650 and 715 nm under the excitation of 475 nm blue light which can be assigned to the 4 G5/2→6 Hj/2（j=5, 7, 9, 11） transitions ofSm^（3＋）, respectively. Besides, by increasing the crystallization temperature or the concentration ofSm^（3＋）, the emission lights of the samples located at 565, 605 and 650 nm are intensified significantly. The present results demonstrate that theSm^（3＋）-doped SAS glassceramics are promising luminescence materials for white LED devices by fine controlling and combining of these three green, orange and red lights in appropriate proportion.

关键词： Sm3+-doped SrO-Al2O3-SiO2 glass-ceramic crystallization luminescence properties

Decoding of Raman spectroscopy-encoded suspension arrays based on the detail constraint cycle domain adaptive model

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Journal of Innovative Optical Health Sciences 2022年第4期15卷 116-127页

作者： Yu Yao Kaiwen Xue liwang liu Shanshan Zhu Chengfeng Yue Yanhong Ji School of Physics and Telecommunication Engineering South China Normal University Guangzhou 510006 GuangdongP.R.China

Previous studies have already shown that Raman spectroscopy can be used in the encoding of suspension array ***,almost all existing convolutional neural network-based decoding approaches rely on supervision with ground truth,and may not be well generalized to unseen datasets,which were collected under different experimental conditions,applying with the same coded *** this study,we propose an improved model based on CyCADA,named as Detail constraint Cycle Domain Adaptive Model(DCDA).DCDA implements the clasification of unseen datasets through domain adaptation,adapts representations at the encode level with decoder-share,and enforces coding features while leveraging a feat *** improve detailed structural constraints,DCDA takes downsample connection and skips *** model improves the poor generalization of existing models and saves the cost of the labeling process for unseen target *** with other models,extensive experiments and ablation studies show the superiority of DCDA in terms of classification stability and *** model proposed by the research achieves a classification with an accuracy of 100%when applied in datasets,in which the spectrum in the source domain is far less than the target domain.

关键词： Domain adaption suspension arrays deep learning Raman spectrum generalization.

Comparative proteomic analysis provides insight into a complex regulatory network of taproot formation in radish (Raphanus sativus L.)

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Horticulture Research 2018年第1期5卷 249-262页

作者： Yang Xie Liang Xu Yan Wang Lianxue Fan Yinglong Chen Mingjia Tang Xiaobo Luo liwang liu National Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of MOACollege of HorticultureNanjing Agricultural UniversityNanjing210095PR China The UWA Institute of Agriculture UWA School of Agriculture and EnvironmentThe University of Western AustraliaPerthWA6001Australia

The fleshy taproot of radish is an important storage organ determining its yield and *** thickening is a complex developmental process in ***,the molecular mechanisms governing this process remain unclear at the proteome *** this study,a comparative proteomic analysis was performed to analyze the proteome changes at three developmental stages of taproot thickening using iTRAQ *** total,1862 differentially expressed proteins(DEPs)were identified from 6342 high-confidence proteins,among which 256 up-regulated proteins displayed overlapped accumulation in S1(pre-cortex splitting stage)vs.S2(cortex splitting stage)and S1 vs.S3(expanding stage)pairs,whereas 122 up-regulated proteins displayed overlapped accumulation in S1 vs.S3 and S2 vs.S3 *** Ontology(GO)and pathway enrichment analysis showed that these DEPs were mainly involved in several processes such as“starch and sucrose metabolism”,“plant hormone signal transduction”,and“biosynthesis of secondary metabolites”.A high concordance existed between iTRAQ and RT-qPCR at the mRNA expression ***,association analysis showed that 187,181,and 96 DEPs were matched with their corresponding differentially expressed genes(DEGs)in S1 vs.S2,S1 vs.S3,and S2 vs.S3 comparison,***,several functional proteins including cell division cycle 5-like protein(CDC5),expansin B1(EXPB1),and xyloglucan endotransglucosylase/hydrolase protein 24(XTH24)were responsible for cell division and expansion during radish taproot thickening *** results could facilitate a better understanding of the molecular mechanism underlying taproot thickening,and provide valuable information for the identification of critical genes/proteins responsible for taproot thickening in root vegetable crops.

关键词： metabolism analysis Raphanus

A genome-wide association study uncovers a critical role of the RsPAP2 gene in red-skinned Raphanus sativus L.

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Horticulture Research 2020年第1期7卷 793-805页

作者： Lianxue Fan YanWang Liang Xu Mingjia Tang Xiaoli Zhang Jiali Ying Cui Li Junhui Dong liwang liu National Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of MOARCollege of HorticultureNanjing Agricultural University210095 NanjingPR China

Radish(Raphanus sativus L.)taproot contains high concentrations of flavonoids,including anthocyanins(ATCs),in redskinned ***,little information on the genetic regulation of ATC biosynthesis in radish is available.A genome-wide association study of radish red skin color was conducted using whole-genome sequencing data derived from 179 radish *** R2R3-MYB transcription factor production of anthocyanin pigment 2(PAP2)gene was found in the region associated with a leading SNP located on chromosome *** amino acid sequence encoded by the RsPAP2 gene was different from those of the other published RsMYB genes responsible for the red skin color of *** overexpression of the RsPAP2 gene resulted in ATC accumulation in Arabidopsis and radish,which was accompanied by the upregulation of several ATC-related structural ***2 was found to bind the RsUFGT and RsTT8 promoters,as shown by a dual-luciferase reporter system and a yeast one-hybrid *** promoter activities of the RsANS,RsCHI,RsPAL,and RsUFGT genes could be strongly activated by coinfiltration with RsPAP2 and *** findings showed the effectiveness of GWAS in identifying candidate genes in radish and demonstrated that RsPAP2 could(either directly or together with its cofactor RsTT8)regulate the transcript levels of ATC-related genes to promote ATC biosynthesis,facilitating the genetic enhancement of ATC contents and other related traits in radish.

关键词： Raphanus critical finding

RsERF40 contributes to cold stress tolerance and cell expansion of taproot in radish (Raphanus sativus L.)

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Horticulture Research 2023年第3期10卷 223-234页

作者： Cui Li Baozhen Mao Kai Wang Liang Xu Lianxue Fan Yan Wang Ying Li Yinbo Ma Lun Wang liwang liu National Key Laboratory of Crop Genetics&Germplasm Enhancement and utilization Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of MOARCollege of HorticultureNanjing Agricultural UniversityNanjing 210095China College of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou 225009China

The growth and development of taproots are inhibited by cold stress in radish(Raphanus sativus L.).Ethylene-responsive element binding factors(ERF)are key participators in the cold stress response and growth regulation of ***,the function of ERF genes in cold tolerance and root development in radish remains ***,we showed that the secondary growth of radish taproots was inhibited by cold *** transcriptome analysis demonstrated that the RsERF40 gene is an important regulator of the cold stress response and root growth *** cold tolerance of transgenic Arabidopsis plants overexpressing the RsERF40 gene was significantly *** RsERF40 in the cold-sensitive radish genotype and silencing RsERF40 in the cold-tolerant radish genotype indicated that RsERF40 was beneficial for alleviating oxidative damage under cold stress in *** Arabidopsis seedlings showed an increase in the elongation and radial growth of dark-grown ***-qPCR analysis showed that the expression of the cold-related genes(CORs)RsCOR78 and RsCOR413PM1 and the cell wall strengthening-related genes RsCESA6 and RsEXPB3 was upregulated in transgenic Arabidopsis *** one-hybrid(Y1H)and dual-luciferase reporter assays(DLA)revealed that RsERF40 directly regulates RsCOR78,RsCOR413PM1,RsCESA6 and RsEXPB3 expression,illustrating that RsERF40 enhances cold tolerance and taproot growth by modulating osmotic adjustment and cell wall mechanical strength in *** this study,the RsERF40-regulon was firstly found to be a new cold response pathway independent of the CBF-COR pathway conferring cold stress tolerance with increasing radish taproot *** results provided novel insight into the molecular mechanism underlying cold stress response and would facilitate the genetic improvement of cold tolerance in radish and other root vegetable crops.

关键词： stress Raphanus roots

Melatonin-induced DNA demethylation of metal transporters and antioxidant genes alleviates lead stress in radish plants

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Horticulture Research 2021年第1期8卷 1887-1900页

作者： Mingjia Tang Liang Xu Yan Wang Junhui Dong Xiaoli Zhang Kai Wang Jiali Ying Cui Li liwang liu National Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of MOARCollege of HorticultureNanjing Agricultural UniversityNanjing 210095P.R.China

Melatonin(MT)is a tryptophan-derived natural product that plays a vital role in plant response to abiotic stresses,including heavy metals(HMs).However,it remains elusive how exogenous MT mediates lead(Pb)accumulation and detoxification at the methylation and transcriptional levels in *** this study,decreased Pb accumulation and increased antioxidant enzyme activity were detected under MT treatment in ***-base resolution maps of DNA methylation under Pb stress(Pb200)and Pb plus MT treatment(Pb_50MT)were first *** genome-wide methylation level was increased under Pb stress,while an overall loss of DNA methylation was observed under MT *** differentially methylated region(DMR)-associated genes between Pb_50MT and Pb200 were uniquely enriched in ion binding terms,including cation binding,iron ion binding,and transition metal ion ***-DMRs between Pb200 and Control exhibited a decreasing trend of methylation under Pb_50MT treatment.A few critical upregulated antioxidant genes(e.g.,RsAPX2,RsPOD52 and RsGST)exhibited decreased methylation levels under MT treatment,which enabled the radish plants to scavenge lead-induced reactive oxygen species(ROS)and decrease oxidative ***,several MT-induced HM transporter genes with low methylation(e.g.,RsABCF5,RsYSL7 and RsHMT)and transcription factors(e.g.,RsWRKY41 and RsMYB2)were involved in reducing Pb accumulation in radish *** findings could facilitate comprehensive elucidation of the molecular mechanism underlying MT-mediated Pb accumulation and detoxification in radish and other root vegetable crops.

关键词： Melatonin stress treatment

Establishing VIGS and CRISPR/Cas9 techniques to verify RsPDS function in radish

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Journal of Integrative Agriculture 2024年第5期23卷 1557-1567页

作者： Jiali Ying Yan Wang Liang Xu Tiaojiao Qin Kai Xia Peng Zhang Yinbo Ma Keyun Zhang Lun Wang Junhui Dong Lianxue Fan Yuelin Zhu liwang liu National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization/Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of Ministry of Agriculture and Rural Affairs/College of Horticulture Nanjing Agricultural UniversityNanjing210095China College of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou 225009China College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China

Virus-induced gene silencing(VIGS)and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)systems are effective technologies for rapid and accurate gene function verification in modern plant ***,the investigation of gene silencing and editing in radish remains *** this study,a bleaching phenotype was generated through the knockdown of RsPDS using tobacco rattle virus(TRV)-and turnip yellow mosaic virus(TYMV)-mediated gene silencing *** TYMV-mediated gene silencing efficiency was higher than the TRV-based VIGS system in *** expression level of RsPDS was significantly inhibited using VIGS in'NAU-067'radish *** rootless seedlings of‘NAU-067'were infected with Agrobacterium rhizogenes using the 2300GN-Ubi-RsPDS-Cas9 vector with two target *** adventitious roots were blue with GUs staining,and four of these adventitious roots were edited at target sequence 1 of the RsPDS gene as indicated by Sanger ***,albino lines were generated with ***-mediated transformation of radish *** base substitutions and three base deletions occurred at target sequence 2 in Line 1,and three base insertions and three base substitutions occurred at target sequence 1 in Line *** study shows that VIGS and CRISPR/Cas9 techniques can be employed to precisely verify the biological functions of genes in radish,which will facilitate the genetic improvement of vital horticultural traits in radish breeding programs.

关键词： Raphanus sativus L. VIGS CRISPR/Cas9 Agrobacterium rhizogenes A.tumefaciens RsPDS

全伺服式中等应变率三轴试验系统的研制及应用

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岩石力学与工程学报 2022年第2期41卷 217-227页

作者：李海波刘黎旺李晓锋刘博李俊如中国科学院武汉岩土力学研究所岩土力学与工程国家重点室验室湖北武汉430071 中国科学院大学北京100049

开展中等应变率试验将有利于理解全应变率空间材料力学特性的率效应特征。为弥补国内外中等应变率试验设备的不足,自主研制RMT401全数字伺服式中等应变率三轴试验系统,主要由3个子系统组成:高频轴向加载系统、全数字围压伺服控制系统与... 详细信息

开展中等应变率试验将有利于理解全应变率空间材料力学特性的率效应特征。为弥补国内外中等应变率试验设备的不足,自主研制RMT401全数字伺服式中等应变率三轴试验系统,主要由3个子系统组成:高频轴向加载系统、全数字围压伺服控制系统与数据采集系统。该系统具备轴向加载和围压的伺服控制功能,实现了动态试验中轴向应变率的稳定加载与围压波动的控制。另外,荷载和变形等参数的高精度量测可获取中等应变率范围(10^(-2)~10^(0)s^(-1))岩石的动态力学特性。为研究应变率和围压对岩石材料力学特性的影响,对红砂岩试样开展中等应变率动三轴试验,结果表明:中等应变率加载条件下,试样加载持时的变化范围为数十毫秒至数十秒;应变率的增加将导致加载持时呈指数递减的变化趋势,而围压的增加将导致加载持时整体增加;不同应变率条件下,材料峰值强度的应变率效应特征可通过幂函数方程描述;不同围压条件下,Mohr-Coulomb与Hoek-Brown破坏准则对材料峰值强度均具有较好的拟合效果,红砂岩的黏聚力c=22.8 MPa,内摩擦角φ=38°,材料常数m=12.1;试样破坏模式主要包括单破裂面和双破裂面2种类型,且随应变率和围压的增加逐渐从单破裂面转换为双破裂面类型;双破裂面所对应破裂角的平均值β分别为58.3°与-58.2°,与Mohr-Coulomb理论计算所得潜在破裂面的破裂角β=±64°接近。

关键词：岩石力学中等应变率动三轴试验应变率效应伺服控制围压