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Establishing VIGS and CRISPR/Cas9 techniques to verify RsPDS function in radish

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Journal of Integrative Agriculture 2024年第5期23卷 1557-1567页

作者： Jiali Ying Yan Wang Liang Xu Tiaojiao Qin Kai Xia Peng Zhang Yinbo Ma Keyun Zhang Lun Wang Junhui Dong Lianxue Fan Yuelin Zhu liwang liu National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization/Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of Ministry of Agriculture and Rural Affairs/College of Horticulture Nanjing Agricultural UniversityNanjing210095China College of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou 225009China College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China

Virus-induced gene silencing(VIGS)and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)systems are effective technologies for rapid and accurate gene function verification in modern plant ***,the investigation of gene silencing and editing in radish remains *** this study,a bleaching phenotype was generated through the knockdown of RsPDS using tobacco rattle virus(TRV)-and turnip yellow mosaic virus(TYMV)-mediated gene silencing *** TYMV-mediated gene silencing efficiency was higher than the TRV-based VIGS system in *** expression level of RsPDS was significantly inhibited using VIGS in'NAU-067'radish *** rootless seedlings of‘NAU-067'were infected with Agrobacterium rhizogenes using the 2300GN-Ubi-RsPDS-Cas9 vector with two target *** adventitious roots were blue with GUs staining,and four of these adventitious roots were edited at target sequence 1 of the RsPDS gene as indicated by Sanger ***,albino lines were generated with ***-mediated transformation of radish *** base substitutions and three base deletions occurred at target sequence 2 in Line 1,and three base insertions and three base substitutions occurred at target sequence 1 in Line *** study shows that VIGS and CRISPR/Cas9 techniques can be employed to precisely verify the biological functions of genes in radish,which will facilitate the genetic improvement of vital horticultural traits in radish breeding programs.

关键词： Raphanus sativus L. VIGS CRISPR/Cas9 Agrobacterium rhizogenes A.tumefaciens RsPDS

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基于波速场反演的爆破损伤区范围界定研究

岩石力学与工程学报 2024年第S1期43卷 3257-3266页

作者：傅帅旸李海波吴迪王犇刘黎旺李晓锋中国科学院武汉岩土力学研究所岩土力学与工程国家重点实验室湖北武汉430071 中国科学院大学北京100049 南京理工大学机械工程学院江苏南京210094

岩体爆破损伤区范围的量化界定对于爆破作用下地下工程围岩稳定性评价、基岩开挖损伤控制等具有重要意义。基于多模板快速行进法(multistencils fast marching methods,MSFM)与同步迭代重建技术(simultaneous iterative reconstructive ... 详细信息

岩体爆破损伤区范围的量化界定对于爆破作用下地下工程围岩稳定性评价、基岩开挖损伤控制等具有重要意义。基于多模板快速行进法(multistencils fast marching methods,MSFM)与同步迭代重建技术(simultaneous iterative reconstructive technique,SIRT),提出一种基于波速场反演定量确定岩体爆破损伤区范围的方法。通过对比反演所得波速场与已知波速场模型,验证所提方法的有效性。使用自主研发的OpenFDEM有限-离散元求解器进行爆破、波速测量模拟,采用自编程后处理程序处理波形数据,对爆破后模型的波速场进行重建。结合现行规范中岩体损伤表征的波速变化率判据,对比分析波速场中不同波速变化率区域与爆生裂纹区域。使用爆破损伤程度不同的区域等效半径与爆破孔半径之比,量化爆破损伤的范围。分别以波速变化率10%与15%作为岩体爆破轻微损伤以及完全损伤的阈值,爆破轻微损伤区及爆破损伤区等效半径与爆破孔半径之比分别为11.86与7.2左右。随着爆破荷载的增加,不同程度损伤区的形状逐渐变得不规则,等效半径增大。研究结果可为现场爆破损伤区范围的确定提供参考。

关键词：岩石力学爆破损伤区波速场反演多模板快速行进法同步迭代重建技术有限-离散元模拟

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A state-of-the-art review of mechanical characteristics and cracking processes of pre-cracked rocks under quasi-static compression

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Journal of Rock Mechanics and Geotechnical Engineering 2022年第6期14卷 2034-2057页

作者： liwang liu Haibo Li Xiaofeng Li State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China

The mechanical characteristics and failure behavior of rocks containing flaws or discontinuities have received wide attention in the field of rock *** external loads are applied to rock materials,stress-induced cracks would initiate and propagate from the flaws,ultimately leading to the irreversible failure of *** investigate the cracking behavior and the effect of flaw geometries on the mechanical properties of rock materials,a series of samples containing one,two and multiple flaws have been widely investigated in the *** this paper,the experimental results for pre-cracked rocks under quasistatic compression were systematically *** progressive failure process of intact rocks is briefly described to reveal the background for experiments on samples with ***,the nondestructive measurement techniques utilized in experiments,such as acoustic emission(AE),X-ray computed tomography(CT),and digital image correlation(DIC),are *** mechanical characteristics of rocks with different flaw geometries and under different loading conditions,including the geometry of pre-existing flaws,flaw filling condition and confining pressure,are ***,the cracking process is evaluated from the perspective of crack initiation,coalescence,and failure patterns.

关键词： Pre-cracked rock Crack initiation pattern Crack coalescence pattern Failure pattern

Decoding of Raman spectroscopy-encoded suspension arrays based on the detail constraint cycle domain adaptive model

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Journal of Innovative Optical Health Sciences 2022年第4期15卷 116-127页

作者： Yu Yao Kaiwen Xue liwang liu Shanshan Zhu Chengfeng Yue Yanhong Ji School of Physics and Telecommunication Engineering South China Normal University Guangzhou 510006 GuangdongP.R.China

Previous studies have already shown that Raman spectroscopy can be used in the encoding of suspension array ***,almost all existing convolutional neural network-based decoding approaches rely on supervision with ground truth,and may not be well generalized to unseen datasets,which were collected under different experimental conditions,applying with the same coded *** this study,we propose an improved model based on CyCADA,named as Detail constraint Cycle Domain Adaptive Model(DCDA).DCDA implements the clasification of unseen datasets through domain adaptation,adapts representations at the encode level with decoder-share,and enforces coding features while leveraging a feat *** improve detailed structural constraints,DCDA takes downsample connection and skips *** model improves the poor generalization of existing models and saves the cost of the labeling process for unseen target *** with other models,extensive experiments and ablation studies show the superiority of DCDA in terms of classification stability and *** model proposed by the research achieves a classification with an accuracy of 100%when applied in datasets,in which the spectrum in the source domain is far less than the target domain.

关键词： Domain adaption suspension arrays deep learning Raman spectrum generalization.

RsERF40 contributes to cold stress tolerance and cell expansion of taproot in radish (Raphanus sativus L.)

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Horticulture Research 2023年第3期10卷 223-234页

作者： Cui Li Baozhen Mao Kai Wang Liang Xu Lianxue Fan Yan Wang Ying Li Yinbo Ma Lun Wang liwang liu National Key Laboratory of Crop Genetics&Germplasm Enhancement and utilization Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of MOARCollege of HorticultureNanjing Agricultural UniversityNanjing 210095China College of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou 225009China

The growth and development of taproots are inhibited by cold stress in radish(Raphanus sativus L.).Ethylene-responsive element binding factors(ERF)are key participators in the cold stress response and growth regulation of ***,the function of ERF genes in cold tolerance and root development in radish remains ***,we showed that the secondary growth of radish taproots was inhibited by cold *** transcriptome analysis demonstrated that the RsERF40 gene is an important regulator of the cold stress response and root growth *** cold tolerance of transgenic Arabidopsis plants overexpressing the RsERF40 gene was significantly *** RsERF40 in the cold-sensitive radish genotype and silencing RsERF40 in the cold-tolerant radish genotype indicated that RsERF40 was beneficial for alleviating oxidative damage under cold stress in *** Arabidopsis seedlings showed an increase in the elongation and radial growth of dark-grown ***-qPCR analysis showed that the expression of the cold-related genes(CORs)RsCOR78 and RsCOR413PM1 and the cell wall strengthening-related genes RsCESA6 and RsEXPB3 was upregulated in transgenic Arabidopsis *** one-hybrid(Y1H)and dual-luciferase reporter assays(DLA)revealed that RsERF40 directly regulates RsCOR78,RsCOR413PM1,RsCESA6 and RsEXPB3 expression,illustrating that RsERF40 enhances cold tolerance and taproot growth by modulating osmotic adjustment and cell wall mechanical strength in *** this study,the RsERF40-regulon was firstly found to be a new cold response pathway independent of the CBF-COR pathway conferring cold stress tolerance with increasing radish taproot *** results provided novel insight into the molecular mechanism underlying cold stress response and would facilitate the genetic improvement of cold tolerance in radish and other root vegetable crops.

关键词： stress Raphanus roots

Crystallization and Luminescence Properties of Sm3＋-Doped SrO-Al2O3-SiO2 Glass-Ceramics

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Journal of Wuhan University of Technology(Materials Science) 2017年第5期32卷 1025-1031页

作者：李宏 liu liwang TANG Xiaozhe WANG Qian Paul W Wang 王伟 State Key Laboratory of Silicate Materials for Architectures (Wuhan University of Technology) Wuhan 430070 China Department of Physics Bradley University Peoria 10 6162.5 USA School of Automotive Engineering Wuhan University of Technology Wuhan 430070 China Hubei Province Key Laboratory of Modern Automotive Technology Wuhan 430070 China 5.Hubei Province Automotive Parts Synergy Innovation Center Wuhan 430070 China

The Sm^（3＋）-doped SrO-Al2O3-SiO2（SAS） glass-ceramics with excellent luminescence properties were prepared by batch melting and heat treatment. The crystallization behavior and luminescent properties of the glass-ceramics were investigated by DTA, XRD, SEM and luminescence spectroscopy. The results indicate that the crystal phase precipitated in this system is monocelsian（SrAl2Si2O） and with the increase of nucleation/crystallization temperature, the crystallite increases from 66 % to 79 %. The Sm（3＋）-doped SAS glass-ceramics emit green, orange and red lights centered at 565, 605, 650 and 715 nm under the excitation of 475 nm blue light which can be assigned to the 4 G5/2→6 Hj/2（j=5, 7, 9, 11） transitions ofSm^（3＋）, respectively. Besides, by increasing the crystallization temperature or the concentration ofSm^（3＋）, the emission lights of the samples located at 565, 605 and 650 nm are intensified significantly. The present results demonstrate that theSm^（3＋）-doped SAS glassceramics are promising luminescence materials for white LED devices by fine controlling and combining of these three green, orange and red lights in appropriate proportion.

关键词： Sm3+-doped SrO-Al2O3-SiO2 glass-ceramic crystallization luminescence properties

Anisotropy-free arrayed waveguide gratings on X-cut thin film lithium niobate platform of in-plane anisotropy

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Light(Science & Applications) 2024年第7期13卷 1416-1426页

作者： Junjie Yi Changjian Guo Ziliang Ruan Gengxin Chen Haiqiang Wei liwang Lu Shengqi Gong Xiaofu Pan Xiaowan Shen Xiaowei Guan Daoxin Dai Kangping Zhong liu liu State Key Laboratory of Extreme Photonics and Instrumentation College of Optical Science and EngineeringInternational Research Center for Advanced PhotonicsZhejiang UniversityHangzhou 310058China. Guangdong Provincial Key Laboratory of Optical Information Materials and Technology South China Academy of Advanced OptoelectronicsSouth China Normal UniversityHigher-Education Mega-CenterGuangzhou 510006China. National Center for International Research on Green Optoelectronics South China Normal UniversityGuangzhou 510006China Photonics Research Institute Department of Electrical and Electronic EngineeringThe Hong Kong Polytechnic UniversityHung HomKowloonHong Kong(SAR)China. Jiaxing Key Laboratory of Photonic Sensing&Intelligent Imaging Intelligent Optics&Photonics Research CenterJiaxing Research Institute Zhejiang UniversityJiaxing 314000China

Arrayed waveguide grating is a versatile and scalable integrated light dispersion device,which has been widely adopted in various applications,including,optical communications and optical ***,thin-film lithium niobate emerges as a promising photonic integration platform,due to its ability of shrinking largely the size of typical lithium niobate based optical *** would also enable multifunctional photonic integrated chips on a single lithium niobate ***,due to the intrinsic anisotropy of the material,to build an arrayed waveguide grating on X-cut thin-film lithium niobate has never been ***,a universal strategy to design anisotropyfree dispersive components on a uniaxial in-plane anisotropic photonic integration platform is introduced for the first *** leads to the first implementation of arrayed waveguide gratings on X-cut thin-film lithium niobate with various configurations and *** best insertion loss of 2.4 dB and crosstalk of−24.1 dB is obtained for the fabricated arrayed waveguide grating *** of such arrayed waveguide gratings as a wavelength router and in a wavelength-division multiplexed optical transmission system are also demonstrated.

关键词： waveguide arrayed lithium

A genome-wide association study uncovers a critical role of the RsPAP2 gene in red-skinned Raphanus sativus L.

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Horticulture Research 2020年第1期7卷 793-805页

作者： Lianxue Fan YanWang Liang Xu Mingjia Tang Xiaoli Zhang Jiali Ying Cui Li Junhui Dong liwang liu National Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of MOARCollege of HorticultureNanjing Agricultural University210095 NanjingPR China

Radish(Raphanus sativus L.)taproot contains high concentrations of flavonoids,including anthocyanins(ATCs),in redskinned ***,little information on the genetic regulation of ATC biosynthesis in radish is available.A genome-wide association study of radish red skin color was conducted using whole-genome sequencing data derived from 179 radish *** R2R3-MYB transcription factor production of anthocyanin pigment 2(PAP2)gene was found in the region associated with a leading SNP located on chromosome *** amino acid sequence encoded by the RsPAP2 gene was different from those of the other published RsMYB genes responsible for the red skin color of *** overexpression of the RsPAP2 gene resulted in ATC accumulation in Arabidopsis and radish,which was accompanied by the upregulation of several ATC-related structural ***2 was found to bind the RsUFGT and RsTT8 promoters,as shown by a dual-luciferase reporter system and a yeast one-hybrid *** promoter activities of the RsANS,RsCHI,RsPAL,and RsUFGT genes could be strongly activated by coinfiltration with RsPAP2 and *** findings showed the effectiveness of GWAS in identifying candidate genes in radish and demonstrated that RsPAP2 could(either directly or together with its cofactor RsTT8)regulate the transcript levels of ATC-related genes to promote ATC biosynthesis,facilitating the genetic enhancement of ATC contents and other related traits in radish.

关键词： Raphanus critical finding

全伺服式中等应变率三轴试验系统的研制及应用

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岩石力学与工程学报 2022年第2期41卷 217-227页

作者：李海波刘黎旺李晓锋刘博李俊如中国科学院武汉岩土力学研究所岩土力学与工程国家重点室验室湖北武汉430071 中国科学院大学北京100049

开展中等应变率试验将有利于理解全应变率空间材料力学特性的率效应特征。为弥补国内外中等应变率试验设备的不足,自主研制RMT401全数字伺服式中等应变率三轴试验系统,主要由3个子系统组成:高频轴向加载系统、全数字围压伺服控制系统与... 详细信息

开展中等应变率试验将有利于理解全应变率空间材料力学特性的率效应特征。为弥补国内外中等应变率试验设备的不足,自主研制RMT401全数字伺服式中等应变率三轴试验系统,主要由3个子系统组成:高频轴向加载系统、全数字围压伺服控制系统与数据采集系统。该系统具备轴向加载和围压的伺服控制功能,实现了动态试验中轴向应变率的稳定加载与围压波动的控制。另外,荷载和变形等参数的高精度量测可获取中等应变率范围(10^(-2)~10^(0)s^(-1))岩石的动态力学特性。为研究应变率和围压对岩石材料力学特性的影响,对红砂岩试样开展中等应变率动三轴试验,结果表明:中等应变率加载条件下,试样加载持时的变化范围为数十毫秒至数十秒;应变率的增加将导致加载持时呈指数递减的变化趋势,而围压的增加将导致加载持时整体增加;不同应变率条件下,材料峰值强度的应变率效应特征可通过幂函数方程描述;不同围压条件下,Mohr-Coulomb与Hoek-Brown破坏准则对材料峰值强度均具有较好的拟合效果,红砂岩的黏聚力c=22.8 MPa,内摩擦角φ=38°,材料常数m=12.1;试样破坏模式主要包括单破裂面和双破裂面2种类型,且随应变率和围压的增加逐渐从单破裂面转换为双破裂面类型;双破裂面所对应破裂角的平均值β分别为58.3°与-58.2°,与Mohr-Coulomb理论计算所得潜在破裂面的破裂角β=±64°接近。

关键词：岩石力学中等应变率动三轴试验应变率效应伺服控制围压