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Localization of orphan receptor TR3 mRNA in early developmental follicles in rat

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Chinese Science Bulletin 2000年第12期45卷 1122-1127页

作者： Haizhen liu yixun liu Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

By in situ hybridization, the localization of orphan receptor TR3 mRNA has been observed in early developmental follicles. TR3 mRNA is first expressed in the ovarian interstitial cells on day 2 after birth, and then in granulosa cells (GC) in primary follicles on day 4. The expression level of TR3 mRNA in GC increases following the follicular development. Its higher expression can be observed in the outer layer of GC and inner layer of theca cells (TC) on day 6, where the cells present active proliferation and differentiation. The expression of TR3 is in an increasing manner until the large antral follicles on day 30. The mRNA is only expressed in the healthy, but not atretic follicles in adult rat ovaries. Injection of epidermal growth factor (EGF) has dramatically enhanced its expression in the early stage of developmental follicles. It is therefore suggested that TR3 may play a role in regulating growth and differentiation of ovarian somatic cells in the early stage, and its expression is regulated

关键词： orphan receptor TR3 in situ hybridization follicular development granulosa cells epidermal growth factor.

Functional spermatid-like cells derived from the ground-state embryonic stem cells in vitro

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Science China(Life Sciences) 2016年第4期59卷 436-437页

作者： Yan Zhang yixun liu State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences

Embryonic stem cells（ESCs）are pluripotent and can self-assemble to form cell clusters or embryoid bodies（EBs）,which can then differentiate into all cell types of the three germ layers,as among which one is the primordial germ cell（PGCs）（Daley,2007）.In vivo PGCs are the gamete founder cells（the ooctyes and sperms）,which transmit genetic information from one generation to the next generation to maintain mammalian life *** 15%of couples in China are infertile;

关键词： primordial pluripotent infertility embryonic PGCs maintain transmit founder differentiate methylation

Localization and expression of TR3 orphan receptor protein and its mRNA in rat

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Chinese Science Bulletin 1998年第2期43卷 146-149页

作者： Xiaomin Mu yixun liu Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

The cellular localization and specific expression of TR3 mRNA in rat testis were investigated by the method of immunohistochemistry and in situ hybridization. It was demonstrated that orphan receptor TR3 was expressed in a significant amount in rat testis and TR3 protein and mRNA were specifically localized in germ cells, suggesting that TR3 may have an important function in regulating rat germ cell development.

关键词： orphan receptor TR3 immunohistochemistry in situ hybridization spermatogenesis.

Expression of orphan receptor TR2 mRNA in rhesus monkey (Macaca mulatta)testis

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Chinese Science Bulletin 1999年第10期44卷 927-930页

作者： Xiaomin Mu yixun liu Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

The cellular localization and expression of TR2 mRNA in rhesus monkey testis were investigated by the methods of in situ and Northern hybridization using the digoxigenin labeled in vitro transcripted cRNA probe from a TR2 cDNA fragment template and α-32P-dCTP labeled cDNA probe from the same TR2 cDNA fragment. It is demonstrated that TR2 mRNA was localized specifically in the germ cells, and was predominantly expressed in the meiotic more advanced cells. The expression level was various in different seminiferous tubules, and there was stronger expression in the tubules where spermatogenesis was vigorously going on. The expression level of TR2 mRNA was low in the immature rhesus monkey testis and increased dramatically in the adult and varied with a seminiferous cycle. These data suggest for the first time that orphan receptor TR2 may have an important function in regulating spermatogenesis at later stages of germ cell development in rhesus monkey.

关键词： orphan receptor TR2 hybridization localization expression spermatogenesis rhesus monkey.

The molecular mechanism of embryonic stem cell pluripotency maintenance

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Chinese Science Bulletin 2005年第19期50卷 2121-2131页

作者： WANG Qingzhong liu yixun HAN Chunsheng College of Life Science Shandong Normal University Jinan 250014 China State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China

In vitro cultured embryonic stem (ES) cells are derived from the inner cell mass (ICM) of pre-implantation embryos, and are capable of giving rise to all cell and tissue types of the three germ layers upon being injected back into blastocysts. These cells are therefore said to possess pluripo-tency that can be maintained infinitely in culture under op-timal conditions. Such pluripotency maintenance is believed to be due to the symmetrical cleavage of the cells in an un-differentiated state. The pluripotency of ES cells is the basis for their various practical and potential applications. ES cells can be used as donor cells to generate knockout or transgenic animals, as in vitro models of mammalian development, and as cell resources for cell therapy in regenerative medicine. The further success in these applications, particularly in the last two, is dependent on the establishment of a culture sys-tem with components in the medium clearly defined and the subsequent procedures for controlled differentiation of the cells into specific lineages. In turn, elucidating the molecular mechanism for pluripotency maintenance of ES cells is the prerequisite. This paper summarizes the recent progresses in this area, focusing mainly on the LIF/STAT3, BMPs/Smads, canonical Wnt, TGFβ/activin/nodal, PI3K and FGF signaling pathways and the genes such as oct4, nanog that are crucial in ES cell pluripotency maintenance. The regulatory systems of pluripotency maintenance in both mouse and human ES cells are also discussed. We believe that the cross-talkings between these signaling pathways, as well as the regulatory system underlying pluripotency maintenance will be the main focus in the area of ES cell researches in the future.

关键词：胚胎干细胞内部细胞聚集无限扩展微生物层对称分裂

Follicles in pregnant rat ovary are incapable of steroidogenesis

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Chinese Science Bulletin 1999年第19期44卷 1797-1801页

作者： Yijun Chen Hongjuan Gao yixun liu Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

Antral follicles are present in the ovaries throughout gestation. These follicles are 'physically immature' and cannot ovulate under the induction of LH/hCG. The purpose of the present study is to examine whether follicles in pregnant rat ovaries are capable of steroidogen-esis. StAR is believed to be the key regulator of steroid hormone biosynthesis. Antisense

关键词： follicles in pregnant ovary steroidogenic acute regulatory protein luteinizing hormone receptor in situ hybridization.

Expression of steroidogenic acute regulatory protein and its regulation by interferon-gamma in rat corpus luteum

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Chinese Science Bulletin 2000年第23期45卷 2152-2157页

作者： Hongjuan Gao Xinlei Chen Lei Shi yixun liu Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China

The steroidogenic acute regulatory protein (StAR) is the key regulatory protein of steroidogenesis. De novo synthesis of StAR protein is required for intramitochondrial translocation of cholesterol to the cytochrome P450 side chain cleavage enzyme which is located on the matrix side of the inner mitochondrial membrane. This is the rate-limiting step of steroid biosynthesis. Using in situ hybridization and immunohistochemistry we studied StAR expression in various stages of the corpora luteal and its regulation by interferon-gamma (IFNγ) in the adult pseudopregnant rat. The results indicated that expression of StAR in the corpora luteal was correlated with progesteron production and IFNγ was capable of inhibiting its expression.

关键词： corpus luteum StAR in situ hybridization immunohistochemistry IFNγ.

Fate determination of fetal Leydig cells

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Frontiers in Biology 2011年第1期6卷 12-18页

作者： Qing WEN yixun liu Fei GAO State Key Laboratory of Reproduction Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Graduate School of the Chinese Academy of Sciences Beijing 100049China

Leydig cell(LC)is one of the most important somatic cell types in testis,which localized in the interstitium between seminiferous *** major function of Leydig cells is to produce steroid hormone,*** differentiation exhibits a biphasic pattern in rodent testes,which are divided into two different temporal mature populations,fetal Leydig cells(FLCs)and adult Leydig cells(ALCs).FLCs are transiently present in fetal testes and undergo involution or degeneration after *** are completely devoid and replaced by ALCs in adult *** to ALCs,FLCs display unique morphology,ultrastructure and *** origin of FLCs has been debated for many years,but it is still a *** factors have been reported regulating the specification,proliferation and differentiation of *** degenerate in a few weeks postnatally,however,the underlying mechanism is still *** this review,we will focus on the fate determination of FLCs,and summarize the resent progress on the morphology,ultrastructure,function,origin and involution of FLCs.

关键词： fetal Leydig cells adult Leydig cells fate determination

Granulosa cell proliferation differentiation and its role in follicular development

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Chinese Science Bulletin 2005年第23期50卷 2665-2671页

作者： LU Cuiling YANG Wei HU Zhaoyuan liu yixun State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of Sciences Beijing 100080 China

Granulosa cells (GCs) are the most important cells in the ovary that undergo serious changes morphologi- cally and physiologically during the processes of follicular proliferation, differentiation, ovulation, lutenization and atresia. Oocyte (OC) directs GC proliferation and differen- tiation, while GCs influence OC maturation. Many ovarian factors are involved in the regulation of these processes via different molecular mechanisms and signal pathways. P38 MAPK can selectively regulate steroidogenesis in GCs con- trolled by FSH; Transcript factors LRH-1 and DAX-1 play an important role in this process; FSH induces GC prolifera- tion and differentiation by stimulating PCNA and StAR ex- pression and steroidogenesis. Activated ERK1/2 signal pathway may be involved in the FSH-regulated GC prolif- eration and differentiation. Therefore,GC is an ideal model for studying cell proliferation, differentiation and interaction as well as signal transduction. This review briefly summa- rizes the latest data in the literature, including the results achieved in our laboratory.

关键词：粒细胞卵母细胞细胞增殖卵泡发育信号转换